A rapid and eco-friendly method for determination of the main components of gamma-oryzanol in equestrian dietary and nutritional supplements by liquid chromatography-Tandem mass spectrometry.
Abstract: Gamma-oryzanol (GO) has gained special attention in the equine sports industry in recent years due to its touted properties, including the fact that it may cause anabolic effects on muscle growth and reduce fatigue. Many manufactures offer supplements containing GO as a naturally occurring anabolic substance; however, some producers do not declare its presence in product compositions. Taking into consideration the touted properties of GO, its ambiguous effectiveness and the open character of the Prohibited Substances List established by the Fédération Equestre Internationale, there is an urgent need to elaborate procedures for the estimation of horse exposure to GO during supplementation, as well as during routine analysis of supplements. This work describes the development and validation of the method for determination of the four main GO components, i.e., cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, campesteryl ferulate and β-sitosteryl ferulate, in equestrian supplements based on LC-MS/MS after a simple ultrasound-assisted extraction (Eco-Scale score value of 76). The analytical performance achieved satisfactory results in terms of linearity (R > 0.9910), sensitivity (LODs ranged from 0.4 to 1.9 ng/mL), intra- and interday accuracy (from 90.4-115.8%), precision (CV < 9.6%) and recovery (from 87.6-108.6%) for all of the investigated compounds. The method was successfully applied to the analysis of thirty equestrian supplements.
Copyright © 2019 Elsevier B.V. All rights reserved.
Publication Date: 2019-04-28 PubMed ID: 31085396DOI: 10.1016/j.jpba.2019.04.029Google Scholar: Lookup
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- Journal Article
Summary
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This research is about a quick and environmentally-friendly method to identify the primary components of gamma-oryzanol in horse dietary supplements, using liquid chromatography-Tandem mass spectrometry.
Research Purpose and Background
- The purpose of this research is to develop and test a new method for determining the presence of gamma-oryzanol (GO) in horse dietary supplements. GO has recently become popular in the equine sports industry due to claims that it may promote muscle growth and reduce fatigue.
- However, not all producers declare the presence of GO in their product components. The researchers argue that, considering the possible effects of GO, and considering the broad nature of the prohibited substances list set by the Fédération Equestre Internationale, it is important to establish methods to measure horse exposure to GO during supplementation, and for routine analysis of supplements.
Methodology
- The researchers set out to develop and verify a method to identify the four main components of GO—cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, campesteryl ferulate, and β-sitosteryl ferulate—in horse supplements using LC-MS/MS after a simple ultrasound-assisted extraction (Eco-Scale score value of 76).
Results and Findings
- The method created in the study achieved satisfactory results in terms of linearity (R > 0.9910), sensitivity (LODs ranged from 0.4 to 1.9 ng/mL), intra- and interday accuracy (from 90.4-115.8%), precision (CV < 9.6%) and recovery (from 87.6-108.6%) for all the compounds investigated.
- The method was then successfully applied to the analysis of thirty different horse dietary supplements.
Conclusion
- This research offers a fast, eco-friendly method for the detection of gamma-oryzanol in horse supplements, which can be a useful tool for industries supporting equestrian sports, regulatory bodies, as well as horse owners who want to ensure the safety and efficacy of the supplements they provide to their horses.
Cite This Article
APA
Waraksa E, Kowalski K, Kłodzińska E, Rola R, Ciekot J, Filipiak W, Bieńkowski T, Namieśnik J.
(2019).
A rapid and eco-friendly method for determination of the main components of gamma-oryzanol in equestrian dietary and nutritional supplements by liquid chromatography-Tandem mass spectrometry.
J Pharm Biomed Anal, 172, 339-348.
https://doi.org/10.1016/j.jpba.2019.04.029 Publication
Researcher Affiliations
- Gdańsk University of Technology, Faculty of Chemistry, Department of Analytical Chemistry, G. Narutowicza 11/12 Street, 80-233, Gdańsk, Poland; Institute of Sport - National Research Institute, Department of Analytical Chemistry and Instrumental Analysis, Trylogii 2/16 Street, 01-982, Warsaw, Poland. Electronic address: emilia.waraksa@insp.waw.pl.
- Masdiag, Żeromskiego 33 Street, 01-832, Warsaw, Poland.
- Institute of Sport - National Research Institute, Department of Analytical Chemistry and Instrumental Analysis, Trylogii 2/16 Street, 01-982, Warsaw, Poland.
- Masdiag, Żeromskiego 33 Street, 01-832, Warsaw, Poland.
- L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Science, Weigla 12 Street, 53-114, Wrocław, Poland.
- Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Jurasza 2 Street, 85-089, Bydgoszcz, Poland.
- Masdiag, Żeromskiego 33 Street, 01-832, Warsaw, Poland.
- Gdańsk University of Technology, Faculty of Chemistry, Department of Analytical Chemistry, G. Narutowicza 11/12 Street, 80-233, Gdańsk, Poland.
MeSH Terms
- Anabolic Agents / chemistry
- Animals
- Chromatography, High Pressure Liquid / methods
- Coumaric Acids / chemistry
- Dietary Supplements / analysis
- Horses
- Phenylpropionates / chemistry
- Reproducibility of Results
- Tandem Mass Spectrometry / methods
- Testosterone Congeners / chemistry
Citations
This article has been cited 1 times.- Li H, Yang H, Ma C, Zhang W, Xu Q, Chen M, Ma Y. [Determination of three oryzanols in rice by mixed-mode solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry].. Se Pu 2022 Aug;40(8):746-752.
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