A rapid diagnostic assay for eastern equine encephalomyelitis viral RNA.
Abstract: Eastern equine encephalomyelitis virus (EEEV) has been a low-frequency, but serious human and veterinary health problem. Increased frequency of this mosquito-borne virus is anticipated as wetlands are maintained and re-established. Control of EEEV has depended on mosquito abatement in response to increasing frequency of EEEV in the environment. A coupled reverse transcription/polymerase chain reaction assay was designed to rapidly, sensitively, and specifically detect EEEV RNA. The assay successfully detected the viral RNA in a single-blind study of a set of field samples composed of either pooled mosquitoes or bird tissue. These results suggest that it would be practical to use this assay for deciding when and where to implement mosquito abatement.
Publication Date: 1993-12-01 PubMed ID: 7904131DOI: 10.4269/ajtmh.1993.49.772Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Research Support
- U.S. Gov't
- P.H.S.
- Animal Health
- Diagnosis
- Diagnostic Technique
- Disease control
- Disease Diagnosis
- Disease Prevention
- Disease Surveillance
- Disease Treatment
- Encephalomyelitis
- Epidemiology
- Equine Health
- Field Study
- Infectious Disease
- Mosquito-borne Diseases
- Polymerase Chain Reaction
- Public Health
- RNA
- Veterinary Medicine
- Veterinary Research
- Virus
- Zoonotic Diseases
Summary
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The research presents a diagnostic test developed to quickly and accurately detect the presence of the RNA of the Eastern equine encephalomyelitis virus (EEEV) in field samples obtained from mosquitoes or bird tissues. This assay’s effectiveness could guide decisions regarding the timing and location of mosquito control measures to curb the spread of EEEV.
Background on EEEV and Need for Assay
- Eastern equine encephalomyelitis virus (EEEV) poses a significant threat to both human and animal health. Although it has been a low-frequency virus, it is serious when it does occur.
- The frequency of this virus is expected to increase as wetlands are maintained and re-established, thereby creating favorable conditions for its mosquito vectors.
- Prevailing control measures for EEEV have relied on mosquito abatement, which is triggered by the observed increase in the virus’s presence in the environment.
- Therefore, there is a need for an efficient and accurate method of detecting EEEV in the environment to inform when and where mosquito abatement should be implemented.
Development and Design of the Diagnostic Assay
- The researchers created an assay that utilizes a coupled reverse transcription/polymerase chain reaction system. This design allows for the rapid, sensitive, and specific detection of EEEV RNA.
- The assay is aimed at enabling a swift identification of the presence of the virus in the environment, which is critical for the timely implementation of control measures.
Evaluation of the Assay’s Effectiveness
- The researchers evaluated this assay in a single-blind study using field samples composed of pooled mosquitoes or bird tissue.
- Results from the study suggested that the assay can successfully detect the viral RNA, making it a potential practical tool for guiding decisions regarding mosquito abatement.
- The usage of this assay could ensure the effective application of prevention strategies, by precisely identifying when and in what locations mosquito reduction efforts should be intensified.
Cite This Article
APA
Vodkin MH, McLaughlin GL, Day JF, Shope RE, Novak RJ.
(1993).
A rapid diagnostic assay for eastern equine encephalomyelitis viral RNA.
Am J Trop Med Hyg, 49(6), 772-776.
https://doi.org/10.4269/ajtmh.1993.49.772 Publication
Researcher Affiliations
- Department of Veterinary Pathobiology, Purdue University, West Lafayette, Indiana.
MeSH Terms
- Animals
- Base Sequence
- Birds
- Culicidae
- DNA Primers / chemistry
- Encephalitis Virus, Eastern Equine / genetics
- Encephalitis Virus, Eastern Equine / isolation & purification
- Female
- Mice
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA, Viral / analysis
- RNA, Viral / isolation & purification
- Restriction Mapping
- Single-Blind Method
- Time Factors
Grant Funding
- AI-10984 / NIAID NIH HHS
- R01-EY082905 / NEI NIH HHS
Citations
This article has been cited 3 times.- de Morais Bronzoni RV, Baleotti FG, Ribeiro Nogueira RM, Nunes M, Moraes Figueiredo LT. Duplex reverse transcription-PCR followed by nested PCR assays for detection and identification of Brazilian alphaviruses and flaviviruses. J Clin Microbiol 2005 Feb;43(2):696-702.
- Linssen B, Kinney RM, Aguilar P, Russell KL, Watts DM, Kaaden OR, Pfeffer M. Development of reverse transcription-PCR assays specific for detection of equine encephalitis viruses. J Clin Microbiol 2000 Apr;38(4):1527-35.
- Pfeffer M, Wiedmann M, Batt CA. Applications of DNA amplification techniques in veterinary diagnostics. Vet Res Commun 1995;19(5):375-407.
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