A review of in vivo and in vitro studies of the mare endometrium.
Abstract: The inner layer of the uterus, the endometrium, is responsible and necessary for many reproductive functions. Normal reproductive cyclicity, maternal recognition of pregnancy, maternal interaction with the embryo, and interaction of the reproductive tract with pathogens are dependent on the endometrium. Although most studies have been conducted in vivo using live animals, recent advances in in vitro approaches could facilitate future research in a laboratory setting with minimal effect on animals. Many reproductive studies have been performed in vivo and in vitro in equids, but new in vitro methods to study the endometrium of mares remain unexplored. In this review, there is a description of the normal anatomy and physiology of the mare endometrium in vivo, in vitro endometrial cell culture techniques that have been previously described for the mare, and opportunities for future reproductive research using in vitro methods.
Copyright © 2020 Elsevier B.V. All rights reserved.
Publication Date: 2020-09-16 PubMed ID: 32987223DOI: 10.1016/j.anireprosci.2020.106605Google Scholar: Lookup
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- Journal Article
- Review
Summary
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This research article reviews existing and potential laboratory methods for studying the endometrium of mares, an important part of equine reproductive system.
Objective of the Research
- The main goal of the research is to provide an exploration into the studies conducted about the mare endometrium, both in vivo (on live animals) and in vitro (in a controlled, external environment like a petri dish).
Relevance of the Endometrium
- The endometrium, the inner layer of the uterus, plays a critical role in various reproductive functions. It is important for normal reproductive cycles, recognizing pregnancy, interacting with the embryo, and dealing with pathogens in the reproductive tract.
Research Approaches
- This research acknowledges the work that has been conducted on living animals (in vivo), but also highlights the importance and potential of in vitro research. In vitro methods allow scientists to conduct studies in a laboratory environment, minimizing the impact on animals.
- In addition, the paper recognizes that while a significant amount of research has been conducted in both settings, there are still areas unexplored when it comes to in vitro studies of the mare’s endometrium.
Structure of the paper
- Within the study, there’s a description of the standard anatomy and physiology of the mare endometrium as observed in an in vivo setting.
- The paper also details in vitro endometrial cell culture techniques that have been previously employed on mares, and prospects for future reproductive investigations using in vitro methods.
Implications for Future Research
- The methods and techniques discussed in this paper provide a roadmap for future research into the mare’s endometrium, particularly when it comes to utilizing new in vitro methods.
Cite This Article
APA
Thompson RE, Premanandan C, Pukazhenthi BS, Whitlock BK.
(2020).
A review of in vivo and in vitro studies of the mare endometrium.
Anim Reprod Sci, 222, 106605.
https://doi.org/10.1016/j.anireprosci.2020.106605 Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, University of Tennessee, Knoxville, TN, USA; Center for Species Survival, Smithsonian Conservation Biology Institute, Front Royal, VA, USA.
- Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA.
- Center for Species Survival, Smithsonian Conservation Biology Institute, Front Royal, VA, USA. Electronic address: pukazhenthib@si.edu.
- Department of Large Animal Clinical Sciences, University of Tennessee, Knoxville, TN, USA. Electronic address: bwhitloc@utk.edu.
MeSH Terms
- Animals
- Cell Culture Techniques / veterinary
- Endometrium / anatomy & histology
- Endometrium / cytology
- Endometrium / physiology
- Estrous Cycle / physiology
- Female
- Horses / anatomy & histology
- Horses / physiology
- Pregnancy
Citations
This article has been cited 3 times.- Fonseca PAS, Suárez-Vega A, Pelayo R, Marina H, Alonso-García M, Gutiérrez-Gil B, Arranz JJ. Intergenerational impact of dietary protein restriction in dairy ewes on epigenetic marks in the perirenal fat of their suckling lambs.. Sci Rep 2023 Mar 16;13(1):4351.
- D'Agostino A, Di Palma T, Cecchini Gualandi S, Boni R. Fluorescence Spectroscopy for the Diagnosis of Endometritis in the Mare.. Animals (Basel) 2022 Apr 29;12(9).
- Diniz WJS, Crouse MS, Cushman RA, McLean KJ, Caton JS, Dahlen CR, Reynolds LP, Ward AK. Cerebrum, liver, and muscle regulatory networks uncover maternal nutrition effects in developmental programming of beef cattle during early pregnancy.. Sci Rep 2021 Feb 2;11(1):2771.
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