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Home / Equine Research Bank / Vet Res Commun / A single-chain fragment variable recombinant antibody agains...
Veterinary research communications2005; 29(6); 463-476; doi: 10.1007/s11259-005-1432-z

A single-chain fragment variable recombinant antibody against F5 fimbria of enterotoxigenic Escherichia coli inhibits agglutination of horse red blood cells induced by F5 protein.

Abstract: Bovine colibacillosis caused by enterotoxigenic Escherichia coli (ETEC) is a worldwide problem. Adhesion of ETEC to intestinal cell receptors mediated by the surface protein F5 fimbriae is the initial step in the establishment of colibacillosis. Prevention of ETEC F5(+) adhesion to enterocytes protects newborn calves against collibacillosis. On the enterocytes, the F5 fimbriae bind to a ganglioside that is also found on horse red blood cells. Thus, the presence of F5 fimbriae induces haemagglutination, which is useful as an indicator in a functional assay system. In this study, recombinant anti-F5 scFv antibody fragment produced in E. coli HB2151 reacted with F5 fimbriae in ELISA and Western immunoblot, and prevented haemagglutination induced by the binding of the F5 fimbriae to its natural host receptors on horse red blood cells. Given the ease with which recombinant antibodies can be mass-produced, the presently described scFv may hold promise as a prophylactic agent for colibacillosis.
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Publication Date: 2005-10-11 PubMed ID: 16215837DOI: 10.1007/s11259-005-1432-zGoogle Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the beneficial use of an anti-F5 antibody fragment called single-chain fragment variable (scFv) in preventing the binding of F5 fimbriae, a part of enterotoxigenic Escherichia coli (ETEC), to host cells. The study suggests this method could potentially serve as a preventative treatment for a condition called colibacillosis.

Research Context

  • Bovine colibacillosis is a wide-ranging issue caused by ETEC. The initial stage of colibacillosis starts with the adherence of ETEC’s F5 fimbriae to intestinal cell receptors. Blocking this adhesion prevents the disease in newborn calves.
  • The F5 fimbriae attach to a ganglioside (a type of molecule) found in horse red blood cells, causing haemagglutination (clumping of red blood cells). Consequently, F5 fimbriae presence helps perform functional assays or experimental procedures.

The Experiment

  • The researchers developed a recombinant anti-F5 scFv antibody fragment in E. coli HB2151. They tested it for its interaction with F5 fimbriae using two common diagnostic techniques: Enzyme-Linked Immunosorbent Assay (ELISA) and Western immunoblot.
  • During the experiment, the scFv antibody successfully prevented haemagglutination resulting from the binding of F5 fimbriae to horse red blood cells.

Implications of the Study

  • The study suggests that the anti-F5 scFv antibody fragment holds potential as a prophylactic agent, meaning it might be used to prevent colibacillosis. It operates by hindering the adhesion of F5 fimbriae to its host receptors, stopping the disease at its starting point.
  • Considering that recombinant antibodies can be reproduced in large quantities, this scFv antibody presents significant promise for disease prevention, forming an accessible and scalable solution for tackling colibacillosis.

Cite This Article

APA
Bhaskaran S, Jay CM, Berghman LR, Wagner GG, Waghela SD. (2005). A single-chain fragment variable recombinant antibody against F5 fimbria of enterotoxigenic Escherichia coli inhibits agglutination of horse red blood cells induced by F5 protein. Vet Res Commun, 29(6), 463-476. https://doi.org/10.1007/s11259-005-1432-z

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 29
Issue: 6
Pages: 463-476

Researcher Affiliations

Bhaskaran, S
  • Department of Veterinary Pathobiology, Texas Veterinary Medical Center, Texas A&M University, College Station, TX 77843, USA.
Jay, C M
    Berghman, L R
      Wagner, G G
        Waghela, S D

          MeSH Terms

          • Amino Acid Sequence
          • Animals
          • Antibodies, Monoclonal / pharmacology
          • Base Sequence
          • Cell Line
          • Erythrocytes / physiology
          • Escherichia coli Proteins / antagonists & inhibitors
          • Escherichia coli Proteins / physiology
          • Fimbriae Proteins / antagonists & inhibitors
          • Fimbriae Proteins / physiology
          • Hemagglutination / physiology
          • Horses
          • Molecular Sequence Data
          • Recombinant Proteins

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          Citations

          This article has been cited 3 times.
          1. Sahagun-Ruiz A, Velazquez LV, Bhaskaran S, Jay CM, Morales-Salinas E, Rathore K, Wagner GG, Waghela SD. Reduction of enterotoxin induced fluid accumulation in ileal loops of neonatal calves with anti-F5 fimbriae recombinant antibody. Vet Res Commun 2015 Dec;39(4):229-36.
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