A study of the ultrastructure and staining characteristics of the ‘dental star’ of equine incisors.

Abstract: The objective of this study was to examine the diameter, extent, orientation and contents of dentinal tubules in order to validate the hypothesis of pigment penetration into the dental star of equine incisival occlusal surfaces. The time of appearance and the configuration of the dental star on the incisival occlusal surface are macroscopically visible features that, along with other more reliable parameters, are used for the determination of horses' age. Although dental stars are an integral part of the equine incisor occlusive surface, the exact nature and microstructure of the dental star are poorly documented. Therefore, equine incisor dentine was examined macroscopically and by scanning electron microscopy to elucidate numerical density, diameter and 3-dimensional organisation of the dentinal tubules in the dental star. The dental star is surrounded by primary dentine and consists of a central core of tertiary dentine, an intermediate ring of pale secondary dentine and a peripheral rim of darker, yellowish-brown secondary dentine. The central core of tertiary dentine contains relatively few dentinal tubules (<8000/mm2) that have small diameters (mean +/- s.d. 1.67 +/- 034 microm) and are arranged in an irregular pattern. The surrounding pale ring of secondary dentine comprises manifestly more and wider tubules that lie almost parallel to the occlusal surface. The dark peripheral rim of the dental star contains high numbers of tubules (28,000-58,000/mm2) that have wide luminal diameters (mean +/- s.d. 3.09 +/- 0.31 microm) and open perpendicular to the occlusal surface. In contrast, the primary dentine surrounding the dental star is made up by a lower number of dentinal tubules (<25,000/mm2). The tubules of primary dentine, which are initially mean +/- s.d. 5.15 +/- 0.80 microm wide, are narrowed by circumferential deposits of peritubular dentine and are obliquely exposed at the occlusal surface. From these observations, it was concluded that the regional differences in numerical density, diameter and spatial orientation of the dentinal tubules may influence the penetration of food pigments into the equine occlusal surface and result in the particular staining of the dental star.