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A Subset of Equine Gastric Squamous Cell Carcinomas Is Associated With Equus Caballus Papillomavirus-2 Infection.
Abstract: Squamous cell carcinoma (SCC) is the most common neoplasm of the equine stomach. However, the mechanisms underlying malignant transformation are unknown. As papillomavirus-2 (EcPV-2) is a likely cause of some genital SCCs, we hypothesized that EcPV-2 is associated with a subset of equine gastric SCCs. To this aim, we performed polymerase chain reaction (PCR) and in situ hybridization (ISH) for EcPV-2 E6/ E7 oncogenes on 11 gastric SCCs and on gastric samples from 15 control horses with no SCC. PCR for EcPV-2 was positive in 7/11 (64%) gastric SCCs; non-SCC gastric samples were all negative. Intense hybridization signals for EcPV-2 E6/E7 nucleic acid were detected by ISH within tumor cells in 5/11 (45%) gastric SCCs, including distant metastases. No hybridization signals were detected within any of the non-SCC gastric cases. This study provides support for a potential association between EcPV-2 infection and a subset of equine gastric SCC.
Publication Date: 2020-03-17 PubMed ID: 32180540PubMed Central: PMC7335262DOI: 10.1177/0300985820908797Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- N.I.H.
- Extramural
Summary
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This research article demonstrates a likely association between Equus Caballus Papillomavirus-2 (EcPV-2) infection and a subset of gastric Squamous Cell Carcinomas (SCCs) in horses. Specifically, EcPV-2 was found in 64% of gastric SCC cases examined in the study but was absent in all control samples from non-SCC horses.
Background
- Squamous Cell Carcinoma (SCC) is a common tumor found in the stomach of horses. However, the process through which it turns malignant remains undefined.
- It has been hypothesized that EcPV-2, similar to its role in some genital SCCs, might be involved in the occurrence of a subset of equine gastric SCCs.
Objective and Methodology
- The researchers conducted polymerase chain reaction (PCR) and in situ hybridization (ISH) tests on 11 gastric SCCs and 15 control gastric samples from horses without SCC. These tests were conducted to check for the presence of EcPV-2 E6/E7 oncogenes which are known to cause cancer.
Results
- EcPV-2 was found in 7 out of the 11 (64%) gastric SCCs tested through PCR.
- In contrast, the virus was not detected in any of the non-SCC gastric samples.
- ISH tests also revealed EcPV-2 E6/E7 nucleic acid in 5 out of the 11 (45%) gastric SCCs, including any distant metastases — tumors that have spread from the primary site to other parts of the body.
- No signs of the virus were found in non-SCC gastric cases through ISH tests.
Conclusion
- The study provides evidence that supports the theory of a potential connection between EcPV-2 infection and a subset of equine gastric SCCs, implying that the virus could play an important role in the development and spread of this type of cancer in horses.
Cite This Article
APA
Alloway E, Linder K, May S, Rose T, DeLay J, Bender S, Tucker A, Luff J.
(2020).
A Subset of Equine Gastric Squamous Cell Carcinomas Is Associated With Equus Caballus Papillomavirus-2 Infection.
Vet Pathol, 57(3), 427-431.
https://doi.org/10.1177/0300985820908797 Publication
Researcher Affiliations
- Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
- Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
- Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
- Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
- Animal Health Laboratory, University of Guelph, Guelph, Ontario, Canada.
- Department of Pathobiology, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA, USA.
- Rollins Animal Disease Diagnostic Laboratory, Raleigh, NC, USA.
- Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
MeSH Terms
- Animals
- Carcinoma, Squamous Cell / genetics
- Carcinoma, Squamous Cell / pathology
- Carcinoma, Squamous Cell / veterinary
- Carcinoma, Squamous Cell / virology
- DNA, Viral / genetics
- Horse Diseases / virology
- Horses
- In Situ Hybridization / veterinary
- Oncogenes / genetics
- Papillomaviridae / genetics
- Papillomaviridae / isolation & purification
- Papillomavirus Infections / pathology
- Papillomavirus Infections / veterinary
- Polymerase Chain Reaction / veterinary
- Stomach / pathology
- Stomach Neoplasms / veterinary
Grant Funding
- K01 OD023219 / NIH HHS
References
This article includes 10 references
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