A technique for magnetic resonance imaging of equine cadaver specimens.
Abstract: We tested an adaptation of a technique for performing magnetic resonance (MR) imaging of human cadaver limbs in the horse. The forelimbs from a normal horse were collected, frozen, and sealed with a paraffin-polymer combination prior to imaging with either a high- or midfield magnetic resonance scanner. Each forelimb was defrosted, scanned, and refrozen on two separate occasions. A five-point scale was used to evaluate the quality of each set of sagittal and transverse, T1-weighted images of each digit. There was no difference in image quality between first and second scans of either specimen (p > 0.05). We conclude that this technique allows investigators to bank tissue specimens for future magnetic resonance imaging without significant loss of image quality.
Publication Date: 1999-02-19 PubMed ID: 10023989DOI: 10.1111/j.1740-8261.1999.tb01832.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The researchers successfully adapted a technique for magnetic resonance imaging from human cadaver limbs to equine limbs. They demonstrated that they could freeze and repeatedly defrost horse limbs without significantly affecting the image quality of their scans.
Research Objectives
- The primary motive behind this research was to test if a technique traditionally used for the magnetic resonance (MR) imaging of human cadaver limbs could be applied effectively to equine cadaver specimens.
Experimental Method
- The forelimbs from a normal horse were collected for the experiment.
- These limbs were then frozen and sealed with a combination of paraffin and polymer to prepare them for imaging.
- The researchers then used a high- or midfield magnetic resonance scanner to image the specimens.
- Furthermore, each limb was defrosted, scanned, and refrozen on two separate occasions to test the consistency of the results.
Scoring and Evaluation
- The researchers used a five-point scale to assess the image quality of both sagittal and transverse, T1-weighted images of each digit in the horse’s forelimb.
- The scores of the first and second scans were then compared to measure the variation in image quality.
Research Findings and Conclusion
- The researchers found no significant difference in the image quality between the first and second scans of either specimen, which suggests that the image quality was maintained after the specimens were defrosted and refrozen.
- This led to the conclusion that this technique offers a viable method for banking tissue specimens for the future use of magnetic resonance imaging without significant degradation in image quality.
Cite This Article
APA
Widmer WR, Buckwalter KA, Hill MA, Fessler JF, Ivancevich S.
(1999).
A technique for magnetic resonance imaging of equine cadaver specimens.
Vet Radiol Ultrasound, 40(1), 10-14.
https://doi.org/10.1111/j.1740-8261.1999.tb01832.x Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, W Lafayette, IN 47907-1532, USA.
MeSH Terms
- Animals
- Cadaver
- Forelimb / anatomy & histology
- Horses / anatomy & histology
- In Vitro Techniques
- Magnetic Resonance Imaging / methods
- Magnetic Resonance Imaging / veterinary
- Male
Citations
This article has been cited 6 times.- Johnston GCA, Ahern BJ, Woldeyohannes SM, Young AC. Does the Low-Field MRI Appearance of Intraosseous STIR Hyperintensity in Equine Cadaver Limbs Change when Subjected to a Freeze-Thaw Process?. Animals (Basel) 2021 Feb 11;11(2).
- Emam H, Aref M, Abdelbaset-Ismail A, Abdelaal A, Gouda S, Gomaa M. Description of normal head structures of the one-humped camel (Camelus dromedarius) by magnetic resonance imaging, computed tomography, and cross-sectional anatomy. Vet World 2020 Aug;13(8):1581-1587.
- Röttiger C, Hellige M, Ohnesorge B, Bienert-Zeit A. Magnetic resonance imaging and computed tomography of equine cheek teeth and adjacent structures: comparative study of image quality in horses in vivo, post-mortem and frozen-thawed. Acta Vet Scand 2019 Dec 10;61(1):62.
- van der Made AD, Maas M, Beenen LF, Oostra RJ, Kerkhoffs GM. Postmortem imaging exposed: an aid in MR imaging of musculoskeletal structures. Skeletal Radiol 2013 Apr;42(4):467-72.
- Arencibia A, Blanco D, González N, Rivero MA. Computed tomography and magnetic resonance imaging features of the temporomandibular joint in two normal camels. Anat Res Int 2012;2012:242065.
- Schmidt S, Mühler M, Schmeling A, Reisinger W, Schulz R. Magnetic resonance imaging of the clavicular ossification. Int J Legal Med 2007 Jul;121(4):321-4.
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