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Accuracy and validation of a point-of-care blood glucose monitoring system for use in horses.
Abstract: Abnormal blood glucose (BG) levels often seen in critically ill horses are significantly associated with adverse patient outcomes and increased mortality. Rapid and accurate BG monitoring is now considered an essential component of evidence-based equine practice and can provide critical information quickly for treatment. Although several point-of-care (POC) BG monitoring hand-held devices are commercially available for veterinary use, none contains a unique algorithm validated for use in horses. The AlphaTrak 3 (AT3) BG monitoring system is a first-of-its-kind device with an equine-specific algorithm that allows stall-side clinical decision making, and frequent monitoring at minimal cost. As such, AT3 is potentially a preferred alternative to more costly and time-consuming standard diagnostic reference laboratory methods. The objective of this study was to determine the accuracy of the AT3 device in measuring BG levels in equine whole blood samples in comparison to results obtained by the Beckman Coulter AU480 reference analyzer per ISO15197:2013 specifications. Accuracy of the AT3 equine algorithm were initially verified by testing equine blood samples with artificially adjusted blood glucose levels followed by its validation in a field study. Testing with artificially adjusted equine samples ( = 129) showed that 98.9% of glucose measurements ranging from 29 to 479 mg/dL fell within ISO accuracy threshold of ±15 mg/dL or ±15% of the average reference value. In addition, 100% of the AT3 measurements fell in consensus error grid (CEG) zone A, which indicates that test outcomes have a minimal likelihood of adverse clinical impact. In a follow-up field study involving 96 horses, 98.4% of AT3 measurements met the ISO accuracy threshold and 99.2% of AT3 measurements fell in CEG zone A. These results demonstrate that the AT3 glucometer has a high degree of accuracy in horses and is a dependable, convenient, and cost-effective device for accurately monitoring equine BG levels in farm or clinical settings.
Copyright © 2024 Velineni, Schiltz, Chang, Peng and Cowles.
Publication Date: 2024-10-10 PubMed ID: 39450406PubMed Central: PMC11500461DOI: 10.3389/fvets.2024.1436714Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research investigates the accuracy of the AlphaTrak 3 blood glucose monitoring system designed specifically for horses and compares the results obtained with those from a reference analyzer. The study demonstrates that this portable device is highly accurate in horses and can be used conveniently in both clinical and farm settings.
Research Objective and Methodology
- The aim of this study was to ascertain the precision of the AlphaTrak 3 (AT3) blood glucose monitoring system, which is particularized for equine use.
- An important component of the research was to correlate the results from AT3 with those from the Beckman Coulter AU480 reference analyzer, according to the ISO15197:2013 specifications.
- The methodology employed in the investigation entailed an initial verification of AT3’s equine algorithm by testing equine blood samples with artificially adjusted glucose levels.
- Following this initial verification, a field study was carried out to provide further validation of the AT3’s accuracy.
Results from the Research
- In testing the artificially adjusted equine samples (129 samples in total), 98.9% of glucose measurements fell within the ISO accuracy threshold. This entails a deviation of ±15 mg/dL or ±15% of the average reference value.
- Every measurement achieved using AT3 fell within Zone A of the consensus error grid (CEG). This zone signifies that the probability of test outcomes leading to adverse clinical impact is minimal.
- In the subsequent field study involving 96 horses, AT3 measurements once again met the ISO accuracy threshold with an accuracy level of 98.4%, and 99.2% of the measurements fell within Zone A of the CEG.
- Essentially, these results indicate a strong level of consistency and reliability in the performance of the AT3 device for measuring blood glucose levels in horses.
Significance and Implications of the Research
- From the study’s outcomes, it is evident the AT3 glucometer provides high accuracy in monitoring blood glucose levels in horses. The high level of consistency and dependable results suggest that it’s a reliable device.
- Because of its portability and affordability compared to standard diagnostic methods, it can be effectively used in both clinical and farm settings, which essentially makes the blood glucose monitoring process for horses much more accessible and convenient.
- This tool could potentially revolutionize the monitoring of blood glucose levels in critically ill horses. Rapid and accurate results could enable quicker, evidence-based clinical decisions leading to improved patient outcomes.
Cite This Article
APA
Velineni S, Schiltz P, Chang KH, Peng YM, Cowles B.
(2024).
Accuracy and validation of a point-of-care blood glucose monitoring system for use in horses.
Front Vet Sci, 11, 1436714.
https://doi.org/10.3389/fvets.2024.1436714 Publication
Researcher Affiliations
- Veterinary Medicine Research and Development, Zoetis, Kalamazoo, MI, United States.
- Associate Professor and Director of Equine Studies, William Woods University, Fulton, MO, United States.
- Tyson Bioresearch Inc., Zhubei City, Taiwan.
- Tyson Bioresearch Inc., Zhubei City, Taiwan.
- Equine Technical Services, Zoetis, Parsippany, NJ, United States.
Conflict of Interest Statement
K-HC and Y-MP were employed by Tyson Bioresearch Inc. The authors declare that this study received funding from Zoetis. The funder had the following involvement in the study: design, analysis, interpretation of data, the writing of this article, or the decision to submit it for publication. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Citations
This article has been cited 1 times.- Mlyneková E, Zaťko S, Halo M, Imrich I, Halo M Jr. The Effect of Seasonal Changes in Non-Structural Carbohydrates in Pasture on the Metabolic Profile of Horses with Laminitis. Animals (Basel) 2026 Jan 15;16(2).
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