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Pathogens (Basel, Switzerland)2021; 10(8); 983; doi: 10.3390/pathogens10080983

Active Circulation of Madariaga Virus, a Member of the Eastern Equine Encephalitis Virus Complex, in Northeast Brazil.

Abstract: Madariaga virus (MADV) is a member of the eastern equine encephalitis virus (EEEV) complex that circulates in Central and South America. It is a zoonotic, mosquito-borne pathogen, belonging to the family . Disturbances in the natural transmission cycle of this virus result in outbreaks in equines and humans, leading to high case fatality in the former and acute febrile illness or neurological disease in the latter. Although a considerable amount of knowledge exists on the eco-epidemiology of North American EEEV strains, little is known about MADV. In Brazil, the most recent isolations of MADV occurred in 2009 in the States of Paraíba and Ceará, northeast Brazil. Because of that, health authorities have recommended vaccination of animals in these regions. However, in 2019 an equine encephalitis outbreak was reported in a municipality in Ceará. Here, we present the isolation of MADV from two horses that died in this outbreak. The full-length genome of these viruses was sequenced, and phylogenetic analyses performed. Pathological findings from postmortem examination are also discussed. We conclude that MADV is actively circulating in northeast Brazil despite vaccination programs, and call attention to this arbovirus that likely represents an emerging pathogen in Latin America.
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Publication Date: 2021-08-03 PubMed ID: 34451447PubMed Central: PMC8400464DOI: 10.3390/pathogens10080983Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper explores the active existence and circulation of the Madariaga virus (MADV) in Northeast Brazil. This virus, within the Eastern Equine Encephalitis Virus (EEEV) complex, is known for outbreak episodes in equines and humans, causing severe cases of neurological diseases or acute illnesses. The study emphasizes the need to address this virus despite vaccination efforts, as it has potential risk as an emergent pathogen in Latin America.

Research Context

  • The Madariaga virus (MADV) is a part of the eastern equine encephalitis virus (EEEV) complex and is known to circulate in Central and South America.
  • This virus spreads through mosquitoes and is classified as a zoonotic pathogen, implying it can transmit from animals to humans.
  • Outbreaks and disturbances in the natural transmission cycle of MADV can have fatal consequences, causing severe sickness or neurological diseases in humans and high case fatalities in equines.
  • While there is substantial knowledge on the eco-epidemiology of the North American EEEV strains, information on MADV is relatively scarce.
  • In Brazil, MADV was most recently isolated in 2009 in the states of Paraíba and Ceará, leading health authorities to recommend animal vaccinations in these regions. However, despite these measures, an equine encephalitis outbreak was reported in Ceará in 2019.

Objectives and Findings

  • The research aimed to study the current rate of MADV circulation in Northeast Brazil. For this, the MADV was isolated from two horses that died during the 2019 outbreak in Ceará.
  • The full-length genome of these viruses was sequenced, and a phylogenetic analysis was conducted. This process allowed the researchers to observe the virus’s genetic makeup and understand its evolutionary relationship with other viruses.
  • The findings from postmortem examinations were also discussed to understand the pathological impacts of the virus.

Conclusion and Implications

  • The study concludes that the MADV is actively circulating in Northeast Brazil, despite the implementation of vaccination programs, indicating that the current measures are not adequately controlling the virus.
  • The research raises concerns about this arbovirus which likely poses as an emerging threat in Latin America.
  • Further, the study emphasizes the significance of the MADV, urging increased attention, and more robust preventive measures and research.

Cite This Article

APA
Gil LHVG, Magalhaes T, Santos BSAS, Oliveira LV, Oliveira-Filho EF, Cunha JLR, Fraiha ALS, Rocha BMM, Longo BC, Ecco R, Faria GC, Furtini R, Drumond SRM, Maranhão RPA, Lobato ZIP, Guedes MIMC, Teixeira RBC, Costa EA. (2021). Active Circulation of Madariaga Virus, a Member of the Eastern Equine Encephalitis Virus Complex, in Northeast Brazil. Pathogens, 10(8), 983. https://doi.org/10.3390/pathogens10080983

Publication

Pathogens (Basel, Switzerland)
ISSN: 2076-0817
NlmUniqueID: 101596317
Country: Switzerland
Language: English
Volume: 10
Issue: 8
PII: 983

Researcher Affiliations

Gil, Laura H V G
  • Departamento de Virologia e Terapia Experimental, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-420, Brazil.
Magalhaes, Tereza
  • Departamento de Virologia e Terapia Experimental, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-420, Brazil.
  • Center for Vector-Borne Infectious Diseases, Microbiology, Immunology and Pathology Department, Colorado State University, Fort Collins, CO 80523-1685, USA.
Santos, Beatriz S A S
  • Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Oliveira, Livia V
  • Departamento de Virologia e Terapia Experimental, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-420, Brazil.
Oliveira-Filho, Edmilson F
  • Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.
Cunha, João L R
  • Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Fraiha, Ana L S
  • Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Rocha, Brenda M M
  • Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Longo, Barbara C
  • Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Ecco, Roselene
  • Setor de Patologia, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Faria, Guilherme C
  • Laboratório de Saúde Animal, Instituto Mineiro de Agropecuária, Belo Horizonte 30110-005, Brazil.
Furtini, Ronaldo
  • Laboratório de Saúde Animal, Instituto Mineiro de Agropecuária, Belo Horizonte 30110-005, Brazil.
Drumond, Safira R M
  • Laboratório de Saúde Animal, Instituto Mineiro de Agropecuária, Belo Horizonte 30110-005, Brazil.
Maranhão, Renata P A
  • Clínica de Equinos, Hospital Veterinário, Universidade Federal de Minas Gerais, Belo Horizonte 31310-250, Brazil.
Lobato, Zélia I P
  • Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Guedes, Maria Isabel M C
  • Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Teixeira, Raffaella B C
  • Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa 36571-000, Brazil.
Costa, Erica A
  • Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.

Grant Funding

  • 423115/20162 / Conselho Nacional de Desenvolvimento Cientu00edfico e Tecnolu00f3gico

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

References

This article includes 59 references
  1. Zacks MA, Paessler S. Encephalitic alphaviruses.. Vet Microbiol 2010 Jan 27;140(3-4):281-6.
    doi: 10.1016/j.vetmic.2009.08.023pmc: PMC2814892pubmed: 19775836google scholar: lookup
  2. Carrera JP, Forrester N, Wang E, Vittor AY, Haddow AD, López-Vergès S, Abadía I, Castaño E, Sosa N, Báez C, Estripeaut D, Díaz Y, Beltrán D, Cisneros J, Cedeño HG, Travassos da Rosa AP, Hernandez H, Martínez-Torres AO, Tesh RB, Weaver SC. Eastern equine encephalitis in Latin America.. N Engl J Med 2013 Aug 22;369(8):732-44.
    doi: 10.1056/NEJMoa1212628pmc: PMC3839813pubmed: 23964935google scholar: lookup
  3. Morens DM, Folkers GK, Fauci AS. Eastern Equine Encephalitis Virus - Another Emergent Arbovirus in the United States.. N Engl J Med 2019 Nov 21;381(21):1989-1992.
    doi: 10.1056/NEJMp1914328pubmed: 31747726google scholar: lookup
  4. CASALS J. ANTIGENIC VARIANTS OF EASTERN EQUINE ENCEPHALITIS VIRUS.. J Exp Med 1964 Apr 1;119(4):547-65.
    doi: 10.1084/jem.119.4.547pmc: PMC2137852pubmed: 14151098google scholar: lookup
  5. Brault AC, Powers AM, Chavez CL, Lopez RN, Cachón MF, Gutierrez LF, Kang W, Tesh RB, Shope RE, Weaver SC. Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central, and South America.. Am J Trop Med Hyg 1999 Oct;61(4):579-86.
    doi: 10.4269/ajtmh.1999.61.579pubmed: 10548292google scholar: lookup
  6. Molaei G, Thomas MC, Muller T, Medlock J, Shepard JJ, Armstrong PM, Andreadis TG. Dynamics of Vector-Host Interactions in Avian Communities in Four Eastern Equine Encephalitis Virus Foci in the Northeastern U.S.. PLoS Negl Trop Dis 2016 Jan;10(1):e0004347.
    doi: 10.1371/journal.pntd.0004347pmc: PMC4713425pubmed: 26751704google scholar: lookup
  7. Armstrong PM, Andreadis TG. Eastern equine encephalitis virus in mosquitoes and their role as bridge vectors.. Emerg Infect Dis 2010 Dec;16(12):1869-74.
    doi: 10.3201/eid1612.100640pmc: PMC3294553pubmed: 21122215google scholar: lookup
  8. Dietz WH Jr, Galindo P, Johnson KM. Eastern equine encephalomyelitis in Panama: the epidemiology of the 1973 epizootic.. Am J Trop Med Hyg 1980 Jan;29(1):133-40.
    doi: 10.4269/ajtmh.1980.29.133pubmed: 7352621google scholar: lookup
  9. Sabattini MS, Daffner JF, Monath TP, Bianchi TI, Cropp CB, Mitchell CJ, Aviles G. Localized eastern equine encephalitis in Santiago del Estero Province, Argentina, without human infection.. Medicina (B Aires) 1991;51(1):3-8.
    pubmed: 1921689
  10. Vasconcelos PF, Da Rosa JF, Da Rosa AP, Dégallier N, Pinheiro Fde P, Sá Filho GC. [Epidemiology of encephalitis caused by arbovirus in the Brazilian Amazonia].. Rev Inst Med Trop Sao Paulo 1991 Nov-Dec;33(6):465-76.
    doi: 10.1590/S0036-46651991000600007pubmed: 1844977google scholar: lookup
  11. Medina G, Gleiser CA, Mackenzie RB. [Outbreak of equine encephalomyelitis in the Republic of Panama].. Bol Oficina Sanit Panam 1965 May;58(5):390-4.
    pubmed: 4221180
  12. Arrigo NC, Adams AP, Weaver SC. Evolutionary patterns of eastern equine encephalitis virus in North versus South America suggest ecological differences and taxonomic revision.. J Virol 2010 Jan;84(2):1014-25.
    doi: 10.1128/JVI.01586-09pmc: PMC2798374pubmed: 19889755google scholar: lookup
  13. Alice FJ. Encefalomielite eqüina na Bahia: Estudo de três amostras isoladas.. Rev. Brasil. Biol. 1951;11:125–144.
  14. Alice FJ. Infecção humana pelo vírus “leste” da encefalite equina.. Bol. Inst. Biol. Bahia. 1956;3:3–9.
  15. Causey OR, Shope RE, Sutmoller P, Laemmert H. Epizootic eastern equine encephalitis in the Bragança region of Pará, Brazil.. Rev. Serv. Esp. Saúde Pública. 1962;12:39–45.
  16. de Souza Lopes O, de Abreu Sacchetta L. Epidemiological studies on eastern equine encephalitis virus in São Paulo, Brazil.. Rev Inst Med Trop Sao Paulo 1974 Sep-Oct;16(5):253-8.
    pubmed: 4477404
  17. Aguiar DM, Cavalcante GT, Lara MCCSH, Villalobos EMC, Cunha EMS, Okuda LH, Stefano E, Nassar AFC, Souza GO, Vasconcellos SA. Prevalência de anticorpos contra agentes virais e bacterianos em eqüídeos do Município de Monte Negro, Rondônia, Amazônia Ocidental Brasileira.. Braz. J. Vet. Res. Anim. Sci. 2008;45:269–276.
    doi: 10.11606/issn.1678-4456.bjvras.2008.26685google scholar: lookup
  18. Carneiro V, Cunha R. Estudos sobre a encefalomielite infecciosa dos equídeos no Brasil.. Arch. Inst. Biol. 1943;14:157–194.
  19. Cunha R. Estudos sôbre uma amostra de vírus da encefalomielite equina isolada de material proveniente de Recife.. Bol. Soc. Bras. Med. Vet. 1945;14:201.
  20. Campos KF, Oliveira CHS, Reis AB, Yamasaki EM, Brito MF, Andrade SJT, Duarte MD, Barbosa JD. Surto de encefalomielite equina Leste na Ilha de Marajó, Pará.. Pesq. Vet. Bras. 2013;33:443–448.
    doi: 10.1590/S0100-736X2013000400005google scholar: lookup
  21. de Souza Costa MC, Siqueira Maia LM, Costa de Souza V, Gonzaga AM, Correa de Azevedo V, Ramos Martins L, Chavez Pavoni JH, Gomes Naveca F, Dezengrini Slhessarenko R. Arbovirus investigation in patients from Mato Grosso during Zika and Chikungunya virus introdution in Brazil, 2015-2016.. Acta Trop 2019 Feb;190:395-402.
    doi: 10.1016/j.actatropica.2018.12.019pubmed: 30552880google scholar: lookup
  22. de Novaes Oliveira R, Iamamoto K, Silva ML, Achkar SM, Castilho JG, Ono ED, Lobo RS, Brandão PE, Carnieli P Jr, Carrieri ML, Kotait I, Macedo CI. Eastern equine encephalitis cases among horses in Brazil between 2005 and 2009.. Arch Virol 2014 Oct;159(10):2615-20.
    doi: 10.1007/s00705-014-2121-4pubmed: 24862186google scholar: lookup
  23. Silva MLCR, Auguste AJ, Terzian ACB, Vedovello D, Riet-Correa F, Macário VMK, Mourão MPG, Ullmann LS, Araújo JP Jr, Weaver SC, Nogueira ML. Isolation and Characterization of Madariaga Virus from a Horse in Paraíba State, Brazil.. Transbound Emerg Dis 2017 Jun;64(3):990-993.
    doi: 10.1111/tbed.12441pmc: PMC4879107pubmed: 26608567google scholar: lookup
  24. Lennette EH, Fox JP. Anticorpos neutralizantes para a amostra leste do vírus de encefalomielite equina em equídeos no Brasil.. Mem. Inst. Oswaldo Cruz. 1943;38:85–92.
    doi: 10.1590/S0074-02761943000100009google scholar: lookup
  25. Cunha EMS, Villalobos EMC, Nassar AFC, Lara MCCSH, Peres NF, Palazzo JPC, Silva A, De Stefano E, Pino FA. Prevalência de anticorpos contra agentes virais em equídeos no sul do estado de São Paulo.. Arq. Inst. Biol. São Paulo. 2009;76:165–171.
    doi: 10.1590/1808-1657v76p1652009google scholar: lookup
  26. Iversson LB, Silva RA, da Rosa AP, Barros VL. Circulation of eastern equine encephalitis, western equine encephalitis, Ilhéus, Maguari and Tacaiuma viruses in equines of the Brazilian Pantanal, South America.. Rev Inst Med Trop Sao Paulo 1993 Jul-Aug;35(4):355-9.
    doi: 10.1590/S0036-46651993000400009pubmed: 8115796google scholar: lookup
  27. Pauvolid-Corrêa A, Tavares FN, Costa EV, Burlandy FM, Murta M, Pellegrin AO, Nogueira MF, Silva EE. Serologic evidence of the recent circulation of Saint Louis encephalitis virus and high prevalence of equine encephalitis viruses in horses in the Nhecolândia sub-region in South Pantanal, Central-West Brazil.. Mem Inst Oswaldo Cruz 2010 Sep;105(6):829-33.
    doi: 10.1590/S0074-02762010000600017pubmed: 20945001google scholar: lookup
  28. Fernández Z, Richartz R, Travassos da Rosa A, Soccol VT. [Identification of the encephalitis equine virus, Parana, Brazil].. Rev Saude Publica 2000 Jun;34(3):232-5.
    doi: 10.1590/S0034-89102000000300004pubmed: 10920444google scholar: lookup
  29. Silva ML, Galiza GJ, Dantas AF, Oliveira RN, Iamamoto K, Achkar SM, Riet-Correa F. Outbreaks of Eastern equine encephalitis in northeastern Brazil.. J Vet Diagn Invest 2011 May;23(3):570-5.
    doi: 10.1177/1040638711403414pubmed: 21908293google scholar: lookup
  30. Kmetiuk LB, Custódio de Souza Hunold Lara MDC, Monteforte Cassaro Villalobos E, de Barros Filho IR, Martins CM, Bach RVW, Pistori Machado F, Silva Pereira M, Cavalcante Lipinski L, Dos Santos AP, Biondo AW. Serosurvey of Eastern, Western, and Venezuelan Equine Encephalitis Viruses in Wild Boars (Sus scrofa), Hunting Dogs, and Hunters of Brazil.. Vector Borne Zoonotic Dis 2020 Nov;20(11):868-871.
    doi: 10.1089/vbz.2019.2596pubmed: 32644885google scholar: lookup
  31. Catenacci LS, Ferreira M, Martins LC, De Vleeschouwer KM, Cassano CR, Oliveira LC, Canale G, Deem SL, Tello JS, Parker P, Vasconcelos PFC, Travassos da Rosa ES. Surveillance of Arboviruses in Primates and Sloths in the Atlantic Forest, Bahia, Brazil.. Ecohealth 2018 Dec;15(4):777-791.
    doi: 10.1007/s10393-018-1361-2pubmed: 30117001google scholar: lookup
  32. Bruno-Lobo M, Bruno-Lobo G, Travassos J. Estudos sôbre Arborvírus. II – Presença de anticorpos para certos vírus dos grupos A e B em soros de pessoas residentes no Rio de Janeiro.. An. Microbiol. 1961;9:155–181.
  33. Romano-Lieber NS, Iversson LB. [Serological survey on arbovirus infection in residents of an ecological reserve].. Rev Saude Publica 2000 Jun;34(3):236-42.
    doi: 10.1590/S0034-89102000000300005pubmed: 10920445google scholar: lookup
  34. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.. Mol Biol Evol 2016 Jul;33(7):1870-4.
    doi: 10.1093/molbev/msw054pmc: PMC8210823pubmed: 27004904google scholar: lookup
  35. Cunha R. Verificação de anticorpos para o vírus “Este” da encefalomielite equina em sôro de cavalos no nordeste brasileiro.. Rev. Bras. Biol. 1943;3:425–430.
  36. Blohm GM, Lednicky JA, White SK, Mavian CN, Márquez MC, González-García KP, Salemi M, Morris JG Jr, Paniz-Mondolfi AE. Madariaga Virus: Identification of a Lineage III Strain in a Venezuelan Child With Acute Undifferentiated Febrile Illness, in the Setting of a Possible Equine Epizootic.. Clin Infect Dis 2018 Aug 1;67(4):619-621.
    doi: 10.1093/cid/ciy224pmc: PMC6070041pubmed: 29718127google scholar: lookup
  37. Lednicky JA, White SK, Mavian CN, El Badry MA, Telisma T, Salemi M, OKech BA, Beau De Rochars VM, Morris JG Jr. Emergence of Madariaga virus as a cause of acute febrile illness in children, Haiti, 2015-2016.. PLoS Negl Trop Dis 2019 Jan;13(1):e0006972.
    doi: 10.1371/journal.pntd.0006972pmc: PMC6328082pubmed: 30629592google scholar: lookup
  38. Sousa SKH, Sonne L, Sant’Ana FJF, Reis JL. Encefalomielite equina do leste no Distrito Federal e entorno.. Acta Sci. Vet. 2015;43:1–7.
  39. U.S. Department of Agriculture, Animal and Plant Health Inspection Service Equine Encephalitis (EEE/WEE/VEE) [(accessed on 15 May 2021)]; Available online: https://www.aphis.usda.gov/aphis/ourfocus/animalhealth/animal-disease-information/equine/eee-wee-vee/equine-encephalitis.
  40. Del Piero F, Wilkins PA, Dubovi EJ, Biolatti B, Cantile C. Clinical, pathologic, immunohistochemical, and virologic findings of eastern equine encephalomyelitis in two horses.. Vet Pathol 2001 Jul;38(4):451-6.
    doi: 10.1354/vp.38-4-451pubmed: 11467481google scholar: lookup
  41. Pimentel LA, Oliveira DM, Galiza GJN, Rego RO, Dantas AFM, Riet-Correa F. Doenças do sistema nervoso central de equídeos no semi-árido.. Pesq. Vet. Bras. 2009;29:589–597.
    doi: 10.1590/S0100-736X2009000700015google scholar: lookup
  42. Vandevelde M, Higgins R, Oevermann A. Veterinary Neuropathology: Essentials of Theory and Practice.. Wiley-Blackwell; Hoboken, NJ, USA: 2012.
  43. Cantile C., Youssef S. In: Nervous system. 6th ed. Maxie M.G., editor. Volume 1. Academic Press; New York, NY, USA: 2016. p. 796. Jubb, Kennedy and Palmer’s Pathology of Domestic Animals.
  44. Franklin RP, Kinde H, Jay MT, Kramer LD, Green EG, Chiles RE, Ostlund E, Husted S, Smith J, Parker MD. Eastern equine encephalomyelitis virus infection in a horse from California.. Emerg Infect Dis 2002 Mar;8(3):283-8.
    doi: 10.3201/eid0803.010199pmc: PMC2732474pubmed: 11927026google scholar: lookup
  45. Vittor AY, Armien B, Gonzalez P, Carrera JP, Dominguez C, Valderrama A, Glass GE, Beltran D, Cisneros J, Wang E, Castillo A, Moreno B, Weaver SC. Epidemiology of Emergent Madariaga Encephalitis in a Region with Endemic Venezuelan Equine Encephalitis: Initial Host Studies and Human Cross-Sectional Study in Darien, Panama.. PLoS Negl Trop Dis 2016 Apr;10(4):e0004554.
    doi: 10.1371/journal.pntd.0004554pmc: PMC4839771pubmed: 27101567google scholar: lookup
  46. Forattini OP, Gomes Ade C, Natal D, Kakitani I, Marucci D. [Food preferences of Culicidae mosquitoes in the Ribeira valley, São Paulo, Brazil].. Rev Saude Publica 1987 Jun;21(3):171-87.
    doi: 10.1590/S0034-89101987000300002pubmed: 3445099google scholar: lookup
  47. Forattini OP, Gomes Ade C, Kakitani I, Marucci D. [Domiciliation of Culex mosquitoes (Melanoconion), in man-made deeply modified environment].. Rev Saude Publica 1991 Aug;25(4):257-66.
    doi: 10.1590/S0034-89101991000400004pubmed: 1820613google scholar: lookup
  48. Luna LG. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology.. 3rd ed. McGraw-Hill; New York, NY, USA: 1968.
  49. Pinheiro GG, Rocha MN, de Oliveira MA, Moreira LA, Andrade Filho JD. Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017⁻2018 in Minas Gerais State, Brazil.. Insects 2019 May 10;10(5).
    doi: 10.3390/insects10050136pmc: PMC6572267pubmed: 31083286google scholar: lookup
  50. McAuley AJ, Beasley DW. Propagation and Titration of West Nile Virus on Vero Cells.. Methods Mol Biol 2016;1435:19-27.
    pubmed: 27188547doi: 10.1007/978-1-4939-3670-0_3google scholar: lookup
  51. Sánchez-Seco MP, Rosario D, Domingo C, Hernández L, Valdés K, Guzmán MG, Tenorio A. Generic RT-nested-PCR for detection of flaviviruses using degenerated primers and internal control followed by sequencing for specific identification.. J Virol Methods 2005 Jun;126(1-2):101-9.
    doi: 10.1016/j.jviromet.2005.01.025pubmed: 15847925google scholar: lookup
  52. Pfeffer M, Proebster B, Kinney RM, Kaaden OR. Genus-specific detection of alphaviruses by a semi-nested reverse transcription-polymerase chain reaction.. Am J Trop Med Hyg 1997 Dec;57(6):709-18.
    doi: 10.4269/ajtmh.1997.57.709pubmed: 9430533google scholar: lookup
  53. Bronzoni RV, Moreli ML, Cruz AC, Figueiredo LT. Multiplex nested PCR for Brazilian Alphavirus diagnosis.. Trans R Soc Trop Med Hyg 2004 Aug;98(8):456-61.
    doi: 10.1016/j.trstmh.2003.09.002pubmed: 15186933google scholar: lookup
  54. ThermoFisher Protocol—Phenol/Chloroform extraction. [(accessed on 13 November 2020)]; Available online: https://www.thermofisher.com/br/en/home/references/protocols/nucleic-acid-purification-and-analysis/dna-extraction-protocols/phenol-chloroform-extraction.html.
  55. VanDevanter DR, Warrener P, Bennett L, Schultz ER, Coulter S, Garber RL, Rose TM. Detection and analysis of diverse herpesviral species by consensus primer PCR.. J Clin Microbiol 1996 Jul;34(7):1666-71.
    doi: 10.1128/jcm.34.7.1666-1671.1996pmc: PMC229091pubmed: 8784566google scholar: lookup
  56. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability.. Mol Biol Evol 2013 Apr;30(4):772-80.
    doi: 10.1093/molbev/mst010pmc: PMC3603318pubmed: 23329690google scholar: lookup
  57. Minh BQ, Schmidt HA, Chernomor O, Schrempf D, Woodhams MD, von Haeseler A, Lanfear R. IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era.. Mol Biol Evol 2020 May 1;37(5):1530-1534.
    doi: 10.1093/molbev/msaa015pmc: PMC7182206pubmed: 32011700google scholar: lookup
  58. Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS. ModelFinder: fast model selection for accurate phylogenetic estimates.. Nat Methods 2017 Jun;14(6):587-589.
    doi: 10.1038/nmeth.4285pmc: PMC5453245pubmed: 28481363google scholar: lookup
  59. Letunic I, Bork P. Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation.. Nucleic Acids Res 2021 Jul 2;49(W1):W293-W296.
    doi: 10.1093/nar/gkab301pmc: PMC8265157pubmed: 33885785google scholar: lookup

Citations

This article has been cited 4 times.
  1. Kafai NM, Janova H, Cain MD, Alippe Y, Muraro S, Sariol A, Elam-Noll M, Klein RS, Diamond MS. Entry receptor LDLRAD3 is required for Venezuelan equine encephalitis virus peripheral infection and neurotropism leading to pathogenesis in mice.. Cell Rep 2023 Aug 29;42(8):112946.
    doi: 10.1016/j.celrep.2023.112946pubmed: 37556325google scholar: lookup
  2. Wanzeller ALM, da Silva FS, Hernández LHA, Barros LJL, Freitas MNO, Santos MM, Gonçalves EJ, Pantoja JAS, Lima CS, Lima MF, Costa LRO, das Chagas LL, Silva IF, da Cunha TCADS, do Nascimento BLS, Vasconcelos HB, da Rosa EST, Rodrigues SG, Azevedo RDSDS, Martins LC, Casseb LMN, Chiang JO, Nunes Neto JP, Cruz ACR, Carvalho VL, Vasconcelos PFDC, da Silva EVP. Isolation of Flaviviruses and Alphaviruses with Encephalitogenic Potential Diagnosed by Evandro Chagas Institute (Pará, Brazil) in the Period of 1954-2022: Six Decades of Discoveries.. Viruses 2023 Apr 10;15(4).
    doi: 10.3390/v15040935pubmed: 37112917google scholar: lookup
  3. Bonilla-Aldana DK, Bonilla Carvajal CD, Moreno-Ramos E, Barboza JJ, Rodriguez-Morales AJ. Mapping Eastern (EEE) and Venezuelan Equine Encephalitides (VEE) among Equines Using Geographical Information Systems, Colombia, 2008-2019.. Viruses 2023 Mar 8;15(3).
    doi: 10.3390/v15030707pubmed: 36992416google scholar: lookup
  4. Solís MB, Albuja AC, Villamar MF. "Parenthesis sign" in Eastern equine encephalitis.. Arq Neuropsiquiatr 2022 Sep;80(9):975-976.
    doi: 10.1055/s-0042-1755216pubmed: 36261125google scholar: lookup
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