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The Veterinary clinics of North America. Equine practice2006; 22(3); 663-676; doi: 10.1016/j.cveq.2006.07.002

Advanced methods for handling and preparation of stallion semen.

Abstract: Clinical reproduction in the horse more closely parallels human clinical reproduction than in other domestic farm animals. Horse breeders rarely include fertility as a selection criterion when making mating decisions; in most breeds, there is no licensing or approval of stallions. This has led to a significant number of stallions in the breeding pool that possess desirable performance traits but are subfertile for a variety of reasons, some of them genetically transmitted between generations. Therefore, semen characteristics can vary greatly among stallions within the breeding population. A champion stallion is not gelded or culled for poor semen quality or the inability of his spermatozoa to withstand semen preservation techniques. Rather, equine theriogenologists go to great lengths to maximize reproductive performance using any and all means available. Therefore, advanced methods for processing and selecting stallion semen provide the clinician with valuable tools for handling poor-quality semen or for obtaining spermatozoa for assisted reproduction procedures.
Publication Date: 2006-11-30 PubMed ID: 17129794DOI: 10.1016/j.cveq.2006.07.002Google Scholar: Lookup
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Summary

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This research article discusses the different advanced methods for handling and preparing stallion semen to maximize reproductive performance. The study highlights the importance of these methods as a solution to the inherent issues of poor semen quality in stallions, especially for those with sought-after performance traits.

Background of the Study

  • The authors begin the paper by drawing a comparison between horse reproduction and human clinical reproduction, providing a contextual background that helps us understand the methods used in horse breeding.
  • They mention how horse breeders usually do not consider fertility as a key attribute for mating decisions, focusing more on performance traits. This practice leads to a large number of subfertile stallions within the breeding population, which affects the overall quality of stallion semen.
  • The main challenge here is that a high-performing stallion, even if it produces poor quality semen, is not usually discarded or neutered due to the value of its performance traits.

Dealing with Semen Quality Issue

  • The paper asserts that in such scenarios, where stallions are not neutered despite their poor-quality semen, equine theriogenologists (veterinary professionals specializing in animal reproduction) use advanced methods to increase reproductive efficiency.
  • These methods can significantly help in handling poor-quality semen and enable the extraction of spermatozoa for assisted reproduction procedures. These procedures can be vitally important in ensuring successful horse breeding despite the inherent subfertility issues.

Conclusion

  • This study is significant as it provides insight into advanced techniques for handling and preparing stallion semen to maximize reproductive performance.
  • It underscores the need for alternative approaches to tackle the challenges posed by selective stallion breeding practices, opening up potential avenues for further research in this area.

Cite This Article

APA
Loomis PR. (2006). Advanced methods for handling and preparation of stallion semen. Vet Clin North Am Equine Pract, 22(3), 663-676. https://doi.org/10.1016/j.cveq.2006.07.002

Publication

ISSN: 1558-4224
NlmUniqueID: 8511904
Country: United States
Language: English
Volume: 22
Issue: 3
Pages: 663-676

Researcher Affiliations

Loomis, Paul R
  • Select Breeders Service, 1088 Nesbitt Road, Colora, MD 21917, USA. paulloomis@selectbreeders.com

MeSH Terms

  • Animals
  • Breeding / methods
  • Cell Adhesion
  • Centrifugation, Density Gradient / veterinary
  • Cryopreservation / veterinary
  • Horses / physiology
  • Male
  • Reproductive Techniques, Assisted / veterinary
  • Semen / physiology
  • Semen Preservation / methods
  • Semen Preservation / veterinary
  • Spermatozoa / physiology

Citations

This article has been cited 18 times.
  1. Gutiérrez-Cepeda L, Crespo F, Blazquez JC, Serres C. Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation. Animals (Basel) 2023 Jan 22;13(3).
    doi: 10.3390/ani13030382pubmed: 36766271google scholar: lookup
  2. Ramirez-Perez H, Guerrero-Netro HM, Torres-Rodríguez P, Díaz-Durán M, Boeta-Acosta AM, Diaw M. A combination of taurine and caffeine maintains sperm quality in equine semen during chilled storage. J Adv Vet Anim Res 2021 Dec;8(4):635-641.
    doi: 10.5455/javar.2021.h555pubmed: 35106304google scholar: lookup
  3. Yánez-Ortiz I, Catalán J, Delgado-Bermúdez A, Carluccio A, Miró J, Yeste M. Addition of Reduced Glutathione (GSH) to Freezing Medium Reduces Intracellular ROS Levels in Donkey Sperm. Vet Sci 2021 Dec 2;8(12).
    doi: 10.3390/vetsci8120302pubmed: 34941829google scholar: lookup
  4. Catalán J, Yánez-Ortiz I, Gacem S, Papas M, Bonet S, Rodríguez-Gil JE, Yeste M, Miró J. The Effects of Red Light on Mammalian Sperm Rely upon the Color of the Straw and the Medium Used. Animals (Basel) 2021 Jan 8;11(1).
    doi: 10.3390/ani11010122pubmed: 33429933google scholar: lookup
  5. Catalán J, Papas M, Trujillo-Rojas L, Blanco-Prieto O, Bonilla-Correal S, Rodríguez-Gil JE, Miró J, Yeste M. Red LED Light Acts on the Mitochondrial Electron Chain of Donkey Sperm and Its Effects Depend on the Time of Exposure to Light. Front Cell Dev Biol 2020;8:588621.
    doi: 10.3389/fcell.2020.588621pubmed: 33365309google scholar: lookup
  6. Ponthier J, Rigaux G, Parrilla-Hernandez S, Egyptien S, Gatez C, Carrasco Leroy C, Deleuze S. Case Report: Suppression of Harem Stallion Behavior and Fertility Following Anti-Gonadotropin-Releasing Hormone Vaccination of a Captive Wild Przewalski's Horse (Equus ferus przewalskii). Front Vet Sci 2020;7:569185.
    doi: 10.3389/fvets.2020.569185pubmed: 33330690google scholar: lookup
  7. Catalán J, Papas M, Gacem S, Mateo-Otero Y, Rodríguez-Gil JE, Miró J, Yeste M. Red-Light Irradiation of Horse Spermatozoa Increases Mitochondrial Activity and Motility through Changes in the Motile Sperm Subpopulation Structure. Biology (Basel) 2020 Aug 29;9(9).
    doi: 10.3390/biology9090254pubmed: 32872467google scholar: lookup
  8. Buss T, Aurich J, Aurich C. Evaluation of a portable device for assessment of motility in stallion semen. Reprod Domest Anim 2019 Mar;54(3):514-519.
    doi: 10.1111/rda.13390pubmed: 30592335google scholar: lookup
  9. Gil L, Galindo-Cardiel I, Malo C, González N, Alvarez C. Effect of Cholesterol and Equex-STM Addition to an Egg Yolk Extender on Pure Spanish Stallion Cryopreserved Sperm. ISRN Vet Sci 2013;2013:280143.
    doi: 10.1155/2013/280143pubmed: 24416597google scholar: lookup
  10. Gutiérrez-Cepeda L, Fernández A, Crespo F, Ramírez MÁ, Gosálvez J, Serres C. The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA. Acta Vet Scand 2012 Dec 5;54(1):72.
    doi: 10.1186/1751-0147-54-72pubmed: 23217215google scholar: lookup
  11. Akhtar MF, Swelum AA, Wang C. Effect of Monochromatic Red, Blue, and White Light on Reproductive Hormones of Male Donkeys During the Non-Breeding Season. Animals (Basel) 2026 Feb 4;16(3).
    doi: 10.3390/ani16030490pubmed: 41681471google scholar: lookup
  12. Ullah A, Chen W, Shi L, Wang M, Geng M, Na J, Akhtar MF, Khan MZ, Wang C. Challenges and Enhancing Strategies of Equine Semen Preservation: Nutritional and Genetic Perspectives. Vet Sci 2025 Aug 25;12(9).
    doi: 10.3390/vetsci12090807pubmed: 41012733google scholar: lookup
  13. Brito LFC, Linardi RL, Rosales LAS, Balamurugan NS, Hernández-Avilés C, Ramírez-Agámez L. Evaluation of a Chemically Defined, Long-Term Extender for Liquid Storage of Stallion Semen. Reprod Domest Anim 2025 Sep;60(9):e70126.
    doi: 10.1111/rda.70126pubmed: 41002042google scholar: lookup
  14. Zabala SM, Serres C, Montero N, Crespo F, Lorenzo PL, Pérez-Aguilera V, Oliet A, Hijón V, Moreno S, González-Zorn B, Gutiérrez-Cepeda L. Innovative Approaches to Avoid Antibiotic Use in Equine Semen Cryopreservation: Advancing Sustainable Reproductive Technologies. Animals (Basel) 2025 May 9;15(10).
    doi: 10.3390/ani15101368pubmed: 40427246google scholar: lookup
  15. Bisiau C, Moffett P, Graham J, McCue P. Comparison of Nanoparticles and Single-Layer Centrifugation for Separation of Dead from Live Stallion Spermatozoa. Vet Sci 2024 Jul 10;11(7).
    doi: 10.3390/vetsci11070307pubmed: 39057991google scholar: lookup
  16. Lenický M, Sidor E, Dianová L, Tirpák F, Štefunková N, Dżugan M, Halo M Jr, Halo M, Slanina T, Urban I, Bažány D, Greń A, Roychoudhury S, Schneir ER, Massányi P. The effect of bee drone brood on the motility and viability of stallion spermatozoa-an in vitro study. In Vitro Cell Dev Biol Anim 2024 Jun;60(6):596-608.
    doi: 10.1007/s11626-024-00918-ypubmed: 38772999google scholar: lookup
  17. Al-Kass Z, Morrell JM. Freezing Stallion Semen-What Do We Need to Focus on for the Future?. Vet Sci 2024 Feb 2;11(2).
    doi: 10.3390/vetsci11020065pubmed: 38393083google scholar: lookup
  18. Zabala SM, Serres C, Montero N, Crespo F, Lorenzo PL, Pérez-Aguilera V, Galán C, Domínguez-Gimbernat M, Oliet A, Moreno S, González-Zorn B, Gutiérrez-Cepeda L. Strategies to Reduce the Use of Antibiotics in Fresh and Chilled Equine Semen. Animals (Basel) 2024 Jan 5;14(2).
    doi: 10.3390/ani14020179pubmed: 38254348google scholar: lookup