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Home / Equine Research Bank / Equine Vet J / Airway smooth muscle remodelling in mild and moderate equine...
Equine veterinary journal2021; 54(5); 865-874; doi: 10.1111/evj.13514

Airway smooth muscle remodelling in mild and moderate equine asthma.

Abstract: Airway smooth muscle remodelling in severe equine asthma includes both thickening of airway smooth muscle, resulting from hyperplasia and hypertrophy, and changes in contractility. However, airway smooth muscle changes have not been studied in milder forms of the disease. Objective: To investigate bronchial smooth muscle remodelling in horses with mild and moderate asthma (MEA). Methods: Retrospective case-control study. Methods: The endobronchial biopsies from 18 horses with MEA referred to the Equine Hospital of the Université de Montréal and from seven healthy age-matched control horses were studied. The diagnosis was based on clinical signs and bronchoalveolar lavage fluid cytology. Airway smooth muscle cell proliferation was measured by quantifying the expression of the proliferating cell nuclear antigen (PCNA) using immunohistochemistry and histomorphometry. The expression of the (+)insert smooth muscle myosin heavy chain (SMMHC) isoform, an hypercontractile protein, was assessed by RT-qPCR. Results: Expression of the (+)insert SMMHC isoform in airway smooth muscle was approximately 1.5 times greater in horses with MEA compared with controls (P = .02, mean difference 0.01). Although there were no differences between groups in the proliferation of airway smooth muscle cells (P = .4) or myocyte density (P = .3, mean difference -0.6), the percentage of proliferating myocytes was correlated to pulmonary neutrophilia in horses with neutrophilic inflammation (P = .01, r = .80) and to the expression of the (+)insert SMMHC isoform in asthmatic horses (P = .03, r = .66). Conclusions: Small cohorts of horses were studied, and conclusions are limited to the central airways. Conclusions: These results confirm the presence of bronchial smooth muscle remodelling in mild forms of equine asthma and pave the way for the development of biomarkers to measure asthma progression and response to therapy.
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Publication Date: 2021-10-19 PubMed ID: 34529300DOI: 10.1111/evj.13514Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the condition of airway smooth muscle in horses with milder forms of asthma, revealing notable changes in muscle structure and contractility that are similar to those seen in severe equine asthma. The findings could provide potential biomarkers to gauge the progression of asthma and the effectiveness of treatment.

Objectives and Methodology

  • The aim of this study was to examine alterations in bronchial smooth muscle in horses suffering from mild and moderate cases of asthma. This was done through a retrospective case-control study.
  • The case study was conducted involving 18 horses with Mild and Moderate Equine Asthma (MEA) referred to the Equine Hospital of Université de Montréal, and seven healthy horses of the same age were taken as a control group.
  • The diagnosis was decided based on the symptoms and bronchoalveolar lavage fluid cytology.
  • To analyze airway muscle cell proliferation, the research team quantified the expression of the proliferating cell nuclear antigen (PCNA) through immunohistochemistry and histomorphometry.
  • The expression of the hypercontractile protein called (+)insert smooth muscle myosin heavy chain (SMMHC) isoform was evaluated using RT-qPCR.

Results

  • According to the results of this study, airway smooth muscle expression of the (+)insert SMMHC isoform was found to be approximately 1.5 times higher in MEA horses in comparison to the control group.
  • No significant difference was noticed between the groups in terms of airway smooth muscle cell proliferation or myocyte density.
  • However, in horses with neutrophilic inflammation, there was a correlation established between the number of proliferating myocytes and pulmonary neutrophilia.
  • Moreover, an association was also found between the expression of the (+)insert SMMHC isoform and the number of proliferating myocytes in asthmatic horses.

Conclusions

  • The study was only conducted on a small group of horses, and the conclusions drawn were tailored mainly to central airways.
  • Nevertheless, the study was successful in confirming the occurrence of bronchial smooth muscle remodeling in horses with milder forms of asthma.
  • The findings could influence the development of biomarkers to assess the progression of asthma and the response of these horses to therapy.

Cite This Article

APA
Dupuis-Dowd F, Lavoie JP. (2021). Airway smooth muscle remodelling in mild and moderate equine asthma. Equine Vet J, 54(5), 865-874. https://doi.org/10.1111/evj.13514

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 54
Issue: 5
Pages: 865-874

Researcher Affiliations

Dupuis-Dowd, Florence
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
Lavoie, Jean-Pierre
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, Québec, Canada.

MeSH Terms

  • Airway Remodeling
  • Animals
  • Asthma / drug therapy
  • Asthma / veterinary
  • Case-Control Studies
  • Horse Diseases / pathology
  • Horses
  • Hyperplasia / metabolism
  • Hyperplasia / pathology
  • Hyperplasia / veterinary
  • Muscle, Smooth / metabolism
  • Muscle, Smooth / pathology
  • Protein Isoforms / metabolism
  • Protein Isoforms / therapeutic use
  • Retrospective Studies

Grant Funding

  • Universitu00e9 de Montru00e9al
  • Zoetis Canada
  • Fonds du Centenaire of the Universitu00e9 de Montru00e9al
  • PJT-148807 / CIHR
  • PJT-148807 / CIHR

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Citations

This article has been cited 3 times.
  1. Lo Feudo CM, Ferrucci F, Bizzotto D, Dellacà R, Lavoie JP, Stucchi L. Differences in pulmonary function measured by oscillometry between horses with mild-moderate equine asthma and healthy controls. Equine Vet J 2025 May;57(3):619-628.
    doi: 10.1111/evj.14206pubmed: 39134475google scholar: lookup
  2. Höglund N, Rossi H, Javela HM, Oikari S, Nieminen P, Mustonen AM, Airas N, Kärjä V, Mykkänen A. The amount of hyaluronic acid and airway remodelling increase with the severity of inflammation in neutrophilic equine asthma. BMC Vet Res 2024 Jun 25;20(1):273.
    doi: 10.1186/s12917-024-04136-2pubmed: 38918797google scholar: lookup
  3. van den Brom-Spierenburg AJ, Mureșan AN, Westermann CM. Antimicrobial Prescription Behavior in Equine Asthma Cases: An International Survey. Animals (Basel) 2024 Jan 30;14(3).
    doi: 10.3390/ani14030457pubmed: 38338101google scholar: lookup
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