AK-2011 strain for the development of a vaccine against equine rhinopneumonitis.
Abstract: Equine rhinopneumonitis is an acute, highly contagious disease found virtually worldwide. The purpose of the studies presented in this paper is to develop a technology for the manufacture of a cell-derived equine rhinopneumonitis vaccine, as well as to assess the safety and immunogenicity of the newly developed vaccine in laboratory animals model. The object of the studies was the AK-2011 strain isolated from the horses suffering from rhinopneumonitis during an outbreak of abortions. The viability of the AK-2011 strain was assessed using a continuous line of calf trachea cells, a continuous line of calf kidney cells, a continuous line of sheep kidney cells, a continuous line of bovine kidney cells, a continuous line of green monkey kidney cells, a continuous line of Syrian hamster kidney cells, a primary trypsinized culture of horse kidney cells grown in tubes and flasks and the AK-2011 laboratory strain of equine rhinopneumonitis virus with biological activity of 6.0 lg TCID . Sequencing and polymerase chain reaction analysis were performed. The virus isolated from the ORF68 gene in Kazakhstan appeared to be the most similar to the T-953 and 2222-03 strains isolated in the USA and Australia, respectively, in terms of phylogenetics. As to primary infections, cytopathic effects (CPEs) induced by the AK-2011 virus stain (dilution 10 ) in calf trachea and horse kidney cell cultures were stable from the first to tenth passages, with biological activity of 5.75-6.00 lg TCID . CPEs caused by the virus were apparent on days 2-3, further developed intensively and extended to 60-80% of the cell monolayer on days 5-7. The vaccine results can be used to immunize horses on farms against rhinopneumonia, and horses should be immunized twice with an interval of 2-3 months.
© 2022 Wiley-VCH GmbH.
Publication Date: 2022-04-05 PubMed ID: 35315978DOI: 10.1111/tbed.14531Google Scholar: Lookup
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- Journal Article
- Biomechanics
- Equine Coronavirus
- Equine Diseases
- Equine Health
- Equine Herpesvirus
- Equine Infectious Anemia
- Equine Medicine
- Equine Metabolic Syndrome
- Equine model
- Equine Protozoal Myeloencephalitis
- Equine Research
- Equine Rhinitis Virus
- Equine Science
- Equine Studies
- Equine Viral Arteritis
- Equine Wounds
- Equine-Assisted Therapies
- Influenza
- Vaccine development
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article investigates the development of a vaccine for equine rhinopneumonitis, a highly contagious disease in horses, using the AK-2011 strain and assesses its safety and immunogenicity in lab animal models.
Research Objectives and Methodology
- The researchers aim to develop and assess a cell-derived vaccine against equine rhinopneumonitis using the AK-2011 strain.
- The AK-2011 strain was extracted from horses during a rhinopneumonitis outbreak.
- The viability of AK-2011 was assessed through various cell lines including cells from calves, sheep, green monkeys, hamsters, and horses.
- Sequencing and polymerase chain reaction analysis were executed to understand the viral structure and compare it with other strains.
Findings
- The researchers found that the virus is most similar to the T-953 and 2222-03 strains found in the USA and Australia, respectively, when looking at the ORF68 gene.
- Cytopathic effects (CPEs), which are indicative of viral infection, induced by the AK-2011 strain were observed to be stable from the first to tenth passages. These were apparent on days 2-3 and further developed intensively, extending to 60-80% of the cell monolayer on days 5-7.
Implications
- The research results demonstrate possible use for the AK-2011 strain in developing a vaccine against equine rhinopneumonitis.
- A two-step immunization process, with an interval of 2-3 months between doses, using the developed vaccine might help in protecting horses against rhinopneumonia.
Cite This Article
APA
Abisheva A, Abishov A, Khairullaeva K, Shynybayev K, Kalissynov B, Maikhin K, Kydyrmanov A, Karamendin K, Valdovska A, Syrym N.
(2022).
AK-2011 strain for the development of a vaccine against equine rhinopneumonitis.
Transbound Emerg Dis, 69(5), e1972-e1981.
https://doi.org/10.1111/tbed.14531 Publication
Researcher Affiliations
- Laboratory of Cell Cultures and Culture Media, LLP «SPE DiaVak-ABN», Almaty, Kazakhstan.
- Virology laboratory, LLP «SPE DiaVak-ABN», Almaty, Kazakhstan.
- Laboratory of Cell Cultures and Culture Media, LLP «SPE DiaVak-ABN», Almaty, Kazakhstan.
- Virology laboratory, LLP «SPE DiaVak-ABN», Almaty, Kazakhstan.
- Virology laboratory, LLP «SPE DiaVak-ABN», Almaty, Kazakhstan.
- National Reference Veterinary Center Almaty Branch, Almaty, Kazakhstan.
- Laboratory of Viral Ecology, LLP «SPC of Microbiology and Virology», Almaty, Kazakhstan.
- Laboratory of Viral Ecology, LLP «SPC of Microbiology and Virology», Almaty, Kazakhstan.
- Faculty of Veterinary Medicine, Latvia University of Agriculture, Jelgava, Latvia.
- Laboratory of Microbiology, Research Institute for Biological Safety Problems, Gvardeiskiy, Kazakhstan.
MeSH Terms
- Animals
- Australia
- Cattle
- Cattle Diseases
- Chlorocebus aethiops
- Cytopathogenic Effect, Viral
- Female
- Herpesviridae Infections / veterinary
- Horse Diseases / epidemiology
- Horse Diseases / prevention & control
- Horses
- Pregnancy
- Sheep
- Sheep Diseases
- Vaccines
- Viruses
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Citations
This article has been cited 1 times.- Karamendin K, Abishov A, Kydyrmanov A, Akhmetzhanova M. Genome Sequence of the Attenuated Equid Alphaherpesvirus 1 Strain AK-2011, Isolated in Kazakhstan.. Microbiol Resour Announc 2023 Mar 16;12(3):e0132822.
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