alpha-Lipoic acid supplementation enhances heat shock protein production and decreases post exercise lactic acid concentrations in exercised standardbred trotters.
Abstract: Heat shock protein (HSP) expression is an adaptive mechanism against the disruption of cell homeostasis during exercise. Several antioxidant supplementation strategies have been used to enhance tissue protection. In this study, we examined the effects of a redox modulator, alpha-lipoic acid (LA) on HSP responses in six standardbred trotters following intense aerobic exercise. DL-LA supplementation (25 mg kg(-1) d(-1)) for five weeks increased the resting levels of HSP90 (1.02+/-0.155 in control and 1.26+/-0.090 after supplementation in arbitrary units) and the recovery levels of inducible HSP70 (0.89+/-0.056 in control and 1.05+/-0.089 after supplementation in arbitrary units) in skeletal muscle. Furthermore, LA increased skeletal muscle citrate synthase activity at rest and lowered the blood lactate concentration during exercise without any changes in the heart rate. LA had no effect on concentrations of HSP60, HSP25 or GRP75 in skeletal muscle. LA decreased the exercise-induced increases in plasma aspartate aminotransferase and creatine kinase concentrations during recovery. Our results suggest that LA supplementation may enhance tissue protection and increase oxidative capacity of the muscle in horse.
Publication Date: 2009-05-08 PubMed ID: 19427659DOI: 10.1016/j.rvsc.2009.04.009Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study examines the effects of alpha-lipoic acid supplementation on heat shock protein expression and lactic acid levels in standardbred trotters after strenuous exercise. It concludes that this supplementation can increase muscle protection and oxidative capacity in standardbred trotters.
Research Overview
- The authors of this study investigated the effects of alpha-lipoic acid (LA), a redox modulator, on the expression patterns of heat shock proteins (HSPs) in standardbred trotters.
- LA supplementation was administered at a dosage of 25 milligrams per kilogram per day for five weeks, and its impacts on both resting levels of HSP and recovery levels post exercise were measured.
- The researchers also examined LA’s effects on other physiological elements of standardbred trotters such as muscle citrate synthase activity, blood lactate concentration during exercise, heart rate, and levels of other proteins.
Key Findings
- In the standardbred trotters that received LA supplementation, an increase in the resting levels of HSP90 and the recovery levels of inducible HSP70 in their skeletal muscles were observed.
- LA supplementation also resulted in increased activity of skeletal muscle citrate synthase at rest and lower blood lactate concentration during exercise. However, there were no significant changes in the heart rate.
- LA had no significant impact on the concentrations of HSP60, HSP25, or GRP75 in the skeletal muscles of the standardbred trotters.
- Interestingly, the researchers found that LA decreased the exercise-induced increases in both plasma aspartate aminotransferase and creatine kinase concentrations during the recovery period.
Conclusion
- Based on these findings, the authors of this study concluded that alpha-lipoic acid supplementation can enhance the tissue protection and increase the oxidative capacity of standardbred trotters’ muscles.
- This research reveals a promising avenue in exercising horse care and suggests that alpha-lipoic acid supplementation could be a valuable tool in managing horse exercise and recovery.
Cite This Article
APA
Kinnunen S, Hyyppä S, Oksala N, Laaksonen DE, Hannila ML, Sen CK, Atalay M.
(2009).
alpha-Lipoic acid supplementation enhances heat shock protein production and decreases post exercise lactic acid concentrations in exercised standardbred trotters.
Res Vet Sci, 87(3), 462-467.
https://doi.org/10.1016/j.rvsc.2009.04.009 Publication
Researcher Affiliations
- Institute of Biomedicine, Physiology, University of Kuopio, P.O. Box 1627, 70211 Kuopio, Finland.
MeSH Terms
- Animal Feed
- Animals
- Diet / veterinary
- Dietary Supplements
- Female
- Heat-Shock Proteins / metabolism
- Horses / physiology
- Lactic Acid / blood
- Male
- Physical Conditioning, Animal / adverse effects
- Thioctic Acid / pharmacology
Citations
This article has been cited 7 times.- Li M, Ning B, Wang T. The mechanism and prevention of mitochondrial injury after exercise. J Physiol Biochem 2021 May;77(2):215-225.
- Mason SA, Trewin AJ, Parker L, Wadley GD. Antioxidant supplements and endurance exercise: Current evidence and mechanistic insights. Redox Biol 2020 Aug;35:101471.
- Hosnedlova B, Kepinska M, Skalickova S, Fernandez C, Ruttkay-Nedecky B, Peng Q, Baron M, Melcova M, Opatrilova R, Zidkova J, Bjørklund G, Sochor J, Kizek R. Nano-selenium and its nanomedicine applications: a critical review. Int J Nanomedicine 2018;13:2107-2128.
- Varasteh S, Fink-Gremmels J, Garssen J, Braber S. α-Lipoic acid prevents the intestinal epithelial monolayer damage under heat stress conditions: model experiments in Caco-2 cells. Eur J Nutr 2018 Jun;57(4):1577-1589.
- Moura CS, Lollo PC, Morato PN, Risso EM, Amaya-Farfan J. Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise. Food Nutr Res 2017;61(1):1290740.
- Moura CS, Lollo PC, Morato PN, Nisishima LH, Carneiro EM, Amaya-Farfan J. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 2014;9(1):e83437.
- Rhoads RP, Baumgard LH, Suagee JK, Sanders SR. Nutritional interventions to alleviate the negative consequences of heat stress. Adv Nutr 2013 May 1;4(3):267-76.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
