FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
PloS one2014; 9(10); e111324; doi: 10.1371/journal.pone.0111324

Amniotic membrane-derived mesenchymal cells and their conditioned media: potential candidates for uterine regenerative therapy in the horse.

Abstract: Amniotic membrane-derived mesenchymal cells (AMCs) are considered suitable candidates for a variety of cell-based applications. In view of cell therapy application in uterine pathologies, we studied AMCs in comparison to cells isolated from the endometrium of mares at diestrus (EDCs) being the endometrium during diestrus and early pregnancy similar from a hormonal standpoint. In particular, we demonstrated that amnion tissue fragments (AM) shares the same transcriptional profile with endometrial tissue fragments (ED), expressing genes involved in early pregnancy (AbdB-like Hoxa genes), pre-implantation conceptus development (Erα, PR, PGRMC1 and mPR) and their regulators (Wnt7a, Wnt4a). Soon after the isolation, only AMCs express Wnt4a and Wnt7a. Interestingly, the expression levels of prostaglandin-endoperoxide synthase 2 (PTGS2) were found greater in AM and AMCs than their endometrial counterparts thus confirming the role of AMCs as mediators of inflammation. The expression of nuclear progesterone receptor (PR), membrane-bound intracellular progesterone receptor component 1 (PGRMC1) and membrane-bound intracellular progesterone receptor (mPR), known to lead to improved endometrial receptivity, was maintained in AMCs over 5 passages in vitro when the media was supplemented with progesterone. To further explore the potential of AMCs in endometrial regeneration, their capacity to support resident cell proliferation was assessed by co-culturing them with EDCs in a transwell system or culturing in the presence of AMC-conditioned medium (AMC-CM). A significant increase in EDC proliferation rate exhibited the crucial role of soluble factors as mediators of stem cells action. The present investigation revealed that AMCs, as well as their derived conditioned media, have the potential to improve endometrial cell replenishment when low proliferation is associated to pregnancy failure. These findings make AMCs suitable candidates for the treatment of endometrosis in mares.
Get Full Text
Publication Date: 2014-10-31 PubMed ID: 25360561PubMed Central: PMC4216086DOI: 10.1371/journal.pone.0111324Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the potential of amniotic membrane-derived mesenchymal cells (AMCs) and their conditioned media as effective candidates for uterine regenerative therapy, particularly in horses with uterine pathologies, by comparing them with cells isolated from the endometrium of mares.

Overview of the Research

  • The research primarily focuses on the use of amniotic membrane-derived mesenchymal cells (AMCs) for cell-based applications, specifically for uterine regenerative therapy in mares. The study compares these cells with cells isolated from the endometrium of mares in diestrus termed endometrial diestrus cells (EDCs).
  • The researchers focused on how similar the amnion fragments (AM) and endometrial fragments (ED) are on a transcriptional level, examining genes involved in early pregnancy, pre-implantation conceptus development, and their regulators.
  • AMCs and AM displayed elevated expression levels of prostaglandin-endoperoxide synthase 2 (PTGS2), an inflammation mediator, when compared to their endometrial counterparts. Other receptors crucial to improved endometrial receptivity exhibited sustained expression in AMCs during multiple in vitro passages, so long as the media was supplemented with progesterone.

Key Findings

  • The study’s results showed that soon after isolation, only AMCs expressed Wnt4a and Wnt7a, highlighting their unique transcriptional profile.
  • AMCs and their derived conditioned media demonstrated the potential to enhance endometrial cell replenishment, which is crucial when low proliferation correlates to pregnancy failure. This was proven through co-culturing AMCs with EDCs in a transwell system or in the presence of AMC-conditioned medium (AMC-CM), both conditions resulting in a significant increase in EDC proliferation rate due to the action of soluble factors.
  • These findings suggest that the AMCs could be effective in the treatment of endometrosis, a uterine pathology that hampers successful conception in mares.

Significance of the Research

  • This research offers groundbreaking insight into the potential application of AMCs in the treatment of uterine pathologies in horses. The findings have significant implications, particularly in the veterinary sector, where current therapy options for uterine conditions are limited.
  • The research also suggests that the use of AMC-conditioned media could offer an innovative approach to increase endometrial cell proliferation, thereby potentially enhancing pregnancy success rates in mares.

Cite This Article

APA
Corradetti B, Correani A, Romaldini A, Marini MG, Bizzaro D, Perrini C, Cremonesi F, Lange-Consiglio A. (2014). Amniotic membrane-derived mesenchymal cells and their conditioned media: potential candidates for uterine regenerative therapy in the horse. PLoS One, 9(10), e111324. https://doi.org/10.1371/journal.pone.0111324

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 9
Issue: 10
Pages: e111324
PII: e111324

Researcher Affiliations

Corradetti, Bruna
  • Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy.
Correani, Alessio
  • Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy.
Romaldini, Alessio
  • Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy.
Marini, Maria Giovanna
  • Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy.
Bizzaro, Davide
  • Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy.
Perrini, Claudia
  • Large Animal Hospital, Reproduction Unit, Università degli Studi di Milano, Lodi, Italy.
Cremonesi, Fausto
  • Large Animal Hospital, Reproduction Unit, Università degli Studi di Milano, Lodi, Italy; Department of Veterinary Science for Animal Health, Production and Food Safety, Università degli Studi di Milano, Milan, Italy.
Lange-Consiglio, Anna
  • Large Animal Hospital, Reproduction Unit, Università degli Studi di Milano, Lodi, Italy.

MeSH Terms

  • Amnion / cytology
  • Animals
  • Cell Proliferation
  • Culture Media, Conditioned
  • Endometrium / cytology
  • Female
  • Horses
  • Mesenchymal Stem Cells / cytology
  • Pregnancy
  • Regeneration
  • Uterus / cytology
  • Uterus / physiology

Conflict of Interest Statement

The authors have declared that no competing interests exist.

References

This article includes 54 references
  1. Merkt H, Günzel AR. A survey of early pregnancy losses in West German thoroughbred mares.. Equine Vet J 1979 Oct;11(4):256-8.
    pubmed: 540635doi: 10.1111/j.2042-3306.1979.tb01359.xgoogle scholar: lookup
  2. Ginther OJ, Bergfelt DR, Leith GS, Scraba ST. Embryonic loss in mares: Incidence and ultrasonic morphology.. Theriogenology 1985 Jul;24(1):73-86.
    pubmed: 16726060doi: 10.1016/0093-691x(85)90213-4google scholar: lookup
  3. LeBlanc MM. Advances in the diagnosis and treatment of chronic infectious and post-mating-induced endometritis in the mare.. Reprod Domest Anim 2010 Jun;45 Suppl 2:21-7.
    pubmed: 20591061doi: 10.1111/j.1439-0531.2010.01634.xgoogle scholar: lookup
  4. Lange-Consiglio A, Corradetti B, Bizzaro D, Magatti M, Ressel L, Tassan S, Parolini O, Cremonesi F. Characterization and potential applications of progenitor-like cells isolated from horse amniotic membrane.. J Tissue Eng Regen Med 2012 Aug;6(8):622-35.
    pubmed: 21948689doi: 10.1002/term.465google scholar: lookup
  5. Lange-Consiglio A, Corradetti B, Meucci A, Perego R, Bizzaro D, Cremonesi F. Characteristics of equine mesenchymal stem cells derived from amnion and bone marrow: in vitro proliferative and multilineage potential assessment.. Equine Vet J 2013 Nov;45(6):737-44.
    pubmed: 23527626doi: 10.1111/evj.12052google scholar: lookup
  6. Lange-Consiglio A, Tassan S, Corradetti B, Meucci A, Perego R, Bizzaro D, Cremonesi F. Investigating the efficacy of amnion-derived compared with bone marrow-derived mesenchymal stromal cells in equine tendon and ligament injuries.. Cytotherapy 2013 Aug;15(8):1011-20.
    pubmed: 23602577doi: 10.1016/j.jcyt.2013.03.002google scholar: lookup
  7. Lange-Consiglio A, Rossi D, Tassan S, Perego R, Cremonesi F, Parolini O. Conditioned medium from horse amniotic membrane-derived multipotent progenitor cells: immunomodulatory activity in vitro and first clinical application in tendon and ligament injuries in vivo.. Stem Cells Dev 2013 Nov 15;22(22):3015-24.
    pubmed: 23795963doi: 10.1089/scd.2013.0214google scholar: lookup
  8. Dorniak P, Spencer TE. Biological roles of progesterone, prostaglandins, and interferon-tau in endometrial function and conceptus elongation in ruminants.. Anim Reprod 10: 239–251.
  9. Kajihara T, Brosens JJ, Ishihara O. The role of FOXO1 in the decidual transformation of the endometrium and early pregnancy.. Med Mol Morphol 2013 Jun;46(2):61-8.
    pubmed: 23381604doi: 10.1007/s00795-013-0018-zgoogle scholar: lookup
  10. Ponsuksili S, Murani E, Schwerin M, Schellander K, Tesfaye D, Wimmers K. Gene expression and DNA-methylation of bovine pretransfer endometrium depending on its receptivity after in vitro-produced embryo transfer.. PLoS One 2012;7(8):e42402.
    pmc: PMC3428322pubmed: 22952593doi: 10.1371/journal.pone.0042402google scholar: lookup
  11. Feroze-Zaidi F, Fusi L, Takano M, Higham J, Salker MS, Goto T, Edassery S, Klingel K, Boini KM, Palmada M, Kamps R, Groothuis PG, Lam EW, Smith SK, Lang F, Sharkey AM, Brosens JJ. Role and regulation of the serum- and glucocorticoid-regulated kinase 1 in fertile and infertile human endometrium.. Endocrinology 2007 Oct;148(10):5020-9.
    pubmed: 17640988doi: 10.1210/en.2007-0659google scholar: lookup
  12. Bigsby RM, Cunha GR. Estrogen stimulation of deoxyribonucleic acid synthesis in uterine epithelial cells which lack estrogen receptors.. Endocrinology 1986 Jul;119(1):390-6.
    pubmed: 3720669doi: 10.1210/endo-119-1-390google scholar: lookup
  13. Kurita T, Young P, Brody JR, Lydon JP, O'Malley BW, Cunha GR. Stromal progesterone receptors mediate the inhibitory effects of progesterone on estrogen-induced uterine epithelial cell deoxyribonucleic acid synthesis.. Endocrinology 1998 Nov;139(11):4708-13.
    pubmed: 9794483doi: 10.1210/endo.139.11.6317google scholar: lookup
  14. Hwang NS, Zhang C, Hwang YS, Varghese S. Mesenchymal stem cell differentiation and roles in regenerative medicine.. Wiley Interdiscip Rev Syst Biol Med 2009 Jul-Aug;1(1):97-106.
    pubmed: 20835984doi: 10.1002/wsbm.26google scholar: lookup
  15. Daels PF, Hughes JP. The normal estrous cycle.. In: Equine reproduction. A.O McKinnon and J.L. Voss Eds, 6th edition, Wiley-Blackwell Publishing, USA 121–132.
  16. Donofrio G, Franceschi V, Capocefalo A, Cavirani S, Sheldon IM. Bovine endometrial stromal cells display osteogenic properties.. Reprod Biol Endocrinol 2008 Dec 16;6:65.
    pmc: PMC2657796pubmed: 19087287doi: 10.1186/1477-7827-6-65google scholar: lookup
  17. Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue.. Stem Cells 2006 May;24(5):1294-301.
    pubmed: 16410387doi: 10.1634/stemcells.2005-0342google scholar: lookup
  18. Koch TG, Heerkens T, Thomsen PD, Betts DH. Isolation of mesenchymal stem cells from equine umbilical cord blood.. BMC Biotechnol 2007 May 30;7:26.
    pmc: PMC1904213pubmed: 17537254doi: 10.1186/1472-6750-7-26google scholar: lookup
  19. Guest DJ, Ousey JC, Smith MR. Defining the expression of marker genes in equine mesenchymal stromal cells.. Stem Cells Cloning 2008;1:1-9.
    pmc: PMC3781685pubmed: 24198500doi: 10.2147/sccaa.s3824google scholar: lookup
  20. Reed SA, Johnson SE. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.. J Cell Physiol 2008 May;215(2):329-36.
    pubmed: 17929245doi: 10.1002/jcp.21312google scholar: lookup
  21. Bartholomew S, Owens SD, Ferraro GL, Carrade DD, Lara DJ, Librach FA, Borjesson DL, Galuppo LD. Collection of equine cord blood and placental tissues in 40 thoroughbred mares.. Equine Vet J 2009 Nov;41(8):724-8.
    pubmed: 20095217doi: 10.2746/042516409x429446google scholar: lookup
  22. Hoynowski SM, Fry MM, Gardner BM, Leming MT, Tucker JR, Black L, Sand T, Mitchell KE. Characterization and differentiation of equine umbilical cord-derived matrix cells.. Biochem Biophys Res Commun 2007 Oct 19;362(2):347-53.
    pubmed: 17719011doi: 10.1016/j.bbrc.2007.07.182google scholar: lookup
  23. Cremonesi F, Violini S, Lange Consiglio A, Ramelli P, Ranzenigo G, Mariani P. Isolation, in vitro culture and characterization of foal umbilical cord stem cells at birth.. Vet Res Commun 2008 Sep;32 Suppl 1:S139-42.
    pubmed: 18688745doi: 10.1007/s11259-008-9116-0google scholar: lookup
  24. Passeri S, Nocchi F, Lamanna R, Lapi S, Miragliotta V, Giannessi E, Abramo F, Stornelli MR, Matarazzo M, Plenteda D, Urciuoli P, Scatena F, Coli A. Isolation and expansion of equine umbilical cord-derived matrix cells (EUCMCs).. Cell Biol Int 2009 Jan;33(1):100-5.
    pubmed: 18996215doi: 10.1016/j.cellbi.2008.10.012google scholar: lookup
  25. Iacono E, Cunto M, Zambelli D, Ricci F, Tazzari PL, Merlo B. Could fetal fluid and membranes be an alternative source for mesenchymal stem cells (MSCs) in the feline species? A preliminary study.. Vet Res Commun 2012 Jun;36(2):107-18.
    pubmed: 22327440doi: 10.1007/s11259-012-9520-3google scholar: lookup
  26. Corradetti B, Lange-Consiglio A, Barucca M, Cremonesi F, Bizzaro D. Size-sieved subpopulations of mesenchymal stem cells from intervascular and perivascular equine umbilical cord matrix.. Cell Prolif 2011 Aug;44(4):330-42.
    pmc: PMC6496711pubmed: 21645152doi: 10.1111/j.1365-2184.2011.00759.xgoogle scholar: lookup
  27. Kögler G, Sensken S, Airey JA, Trapp T, Müschen M, Feldhahn N, Liedtke S, Sorg RV, Fischer J, Rosenbaum C, Greschat S, Knipper A, Bender J, Degistirici O, Gao J, Caplan AI, Colletti EJ, Almeida-Porada G, Müller HW, Zanjani E, Wernet P. A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential.. J Exp Med 2004 Jul 19;200(2):123-35.
    pmc: PMC2212008pubmed: 15263023doi: 10.1084/jem.20040440google scholar: lookup
  28. Atli MO, Guzeloglu A, Dinc DA. Expression of wingless type (WNT) genes and their antagonists at mRNA levels in equine endometrium during the estrous cycle and early pregnancy.. Anim Reprod Sci 2011 May;125(1-4):94-102.
    pubmed: 21550190doi: 10.1016/j.anireprosci.2011.04.001google scholar: lookup
  29. Chen Q, Zhang Y, Lu J, Wang Q, Wang S, Cao Y, Wang H, Duan E. Embryo-uterine cross-talk during implantation: the role of Wnt signaling.. Mol Hum Reprod 2009 Apr;15(4):215-21.
    pubmed: 19223336doi: 10.1093/molehr/gap009google scholar: lookup
  30. Miller C, Pavlova A, Sassoon DA. Differential expression patterns of Wnt genes in the murine female reproductive tract during development and the estrous cycle.. Mech Dev 1998 Aug;76(1-2):91-9.
    pubmed: 9767131doi: 10.1016/s0925-4773(98)00112-9google scholar: lookup
  31. Hayashi K, Erikson DW, Tilford SA, Bany BM, Maclean JA 2nd, Rucker EB 3rd, Johnson GA, Spencer TE. Wnt genes in the mouse uterus: potential regulation of implantation.. Biol Reprod 2009 May;80(5):989-1000.
    pmc: PMC2804842pubmed: 19164167doi: 10.1095/biolreprod.108.075416google scholar: lookup
  32. Nusse R (accessed 18.12.09), http://www.stanford.edu/~rnusse/wntwindow.
  33. Mohamed OA, Jonnaert M, Labelle-Dumais C, Kuroda K, Clarke HJ, Dufort D. Uterine Wnt/beta-catenin signaling is required for implantation.. Proc Natl Acad Sci U S A 2005 Jun 14;102(24):8579-84.
    pmc: PMC1150820pubmed: 15930138doi: 10.1073/pnas.0500612102google scholar: lookup
  34. Hayashi K, Burghardt RC, Bazer FW, Spencer TE. WNTs in the ovine uterus: potential regulation of periimplantation ovine conceptus development.. Endocrinology 2007 Jul;148(7):3496-506.
    pubmed: 17431004doi: 10.1210/en.2007-0283google scholar: lookup
  35. Ma L, Benson GV, Lim H, Dey SK, Maas RL. Abdominal B (AbdB) Hoxa genes: regulation in adult uterus by estrogen and progesterone and repression in müllerian duct by the synthetic estrogen diethylstilbestrol (DES).. Dev Biol 1998 May 15;197(2):141-54.
    pubmed: 9630742doi: 10.1006/dbio.1998.8907google scholar: lookup
  36. Lim H, Ma L, Ma WG, Maas RL, Dey SK. Hoxa-10 regulates uterine stromal cell responsiveness to progesterone during implantation and decidualization in the mouse.. Mol Endocrinol 1999 Jun;13(6):1005-17.
    pubmed: 10379898doi: 10.1210/mend.13.6.0284google scholar: lookup
  37. Benson GV, Lim H, Paria BC, Satokata I, Dey SK, Maas RL. Mechanisms of reduced fertility in Hoxa-10 mutant mice: uterine homeosis and loss of maternal Hoxa-10 expression.. Development 1996 Sep;122(9):2687-96.
    pubmed: 8787743doi: 10.1242/dev.122.9.2687google scholar: lookup
  38. Tulac S, Nayak NR, Kao LC, Van Waes M, Huang J, Lobo S, Germeyer A, Lessey BA, Taylor RN, Suchanek E, Giudice LC. Identification, characterization, and regulation of the canonical Wnt signaling pathway in human endometrium.. J Clin Endocrinol Metab 2003 Aug;88(8):3860-6.
    pubmed: 12915680doi: 10.1210/jc.2003-030494google scholar: lookup
  39. Kim S, Choi Y, Bazer FW, Spencer TE. Identification of genes in the ovine endometrium regulated by interferon tau independent of signal transducer and activator of transcription 1.. Endocrinology 2003 Dec;144(12):5203-14.
    pubmed: 12960022doi: 10.1210/en.2003-0665google scholar: lookup
  40. Kiewisz J, Kczmarek MM, Blitek A, Bodek G, Ziecik AJ. Expression profile of the Wnt5a, Wnt7a, beta-catenin and E-cadherin genes in the endometrium during peri-implantation period in pigs.. Reprod Dom Anim 43: 92.
  41. Mericskay M, Kitajewski J, Sassoon D. Wnt5a is required for proper epithelial-mesenchymal interactions in the uterus.. Development 2004 May;131(9):2061-72.
    pubmed: 15073149doi: 10.1242/dev.01090google scholar: lookup
  42. Hess AP, Nayak NR, Giudice LC. Oviduct and endometrium: cyclic changes in the primate oviduct and endometrium.. In: Knobil and Neill's Physiology of Reproduction, 3rd edition. Elsevier Academic Press Inc., United States of America Neill JM, (Ed.), 9: 337–382.
  43. Paria BC, Lim H, Das SK, Reese J, Dey SK. Molecular signaling in uterine receptivity for implantation.. Semin Cell Dev Biol 2000 Apr;11(2):67-76.
    pubmed: 10873704doi: 10.1006/scdb.2000.0153google scholar: lookup
  44. de Ziegler D, Fanchin R, de Moustier B, Bulletti C. The hormonal control of endometrial receptivity: estrogen (E2) and progesterone.. J Reprod Immunol 1998 Aug;39(1-2):149-66.
    pubmed: 9786459doi: 10.1016/s0165-0378(98)00019-9google scholar: lookup
  45. Beato M. Gene regulation by steroid hormones.. Cell 1989 Feb 10;56(3):335-44.
    pubmed: 2644044doi: 10.1016/0092-8674(89)90237-7google scholar: lookup
  46. Tuohimaa P, Bläuer M, Pasanen S, Passinen S, Pekki A, Punnonen R, Syvälä H, Valkila J, Wallén M, Väliaho J, Zhuang YH, Ylikomi T. Mechanisms of action of sex steroid hormones: basic concepts and clinical correlations.. Maturitas 1996 May;23 Suppl:S3-12.
    pubmed: 8865132doi: 10.1016/0378-5122(96)01004-3google scholar: lookup
  47. Suzuki T, Schirra F, Richards SM, Jensen RV, Sullivan DA. Estrogen and progesterone control of gene expression in the mouse meibomian gland.. Invest Ophthalmol Vis Sci 2008 May;49(5):1797-808.
    pubmed: 18436814doi: 10.1167/iovs.07-1458google scholar: lookup
  48. Falkenstein E, Norman AW, Wehling M. Mannheim classification of nongenomically initiated (rapid) steroid action(s).. J Clin Endocrinol Metab 2000 May;85(5):2072-5.
    pubmed: 10843198doi: 10.1210/jcem.85.5.6516google scholar: lookup
  49. Rambags BP, van Tol HT, van den Eng MM, Colenbrander B, Stout TA. Expression of progesterone and oestrogen receptors by early intrauterine equine conceptuses.. Theriogenology 2008 Feb;69(3):366-75.
    pubmed: 18037481doi: 10.1016/j.theriogenology.2007.10.011google scholar: lookup
  50. Han K, Lee JE, Kwon SJ, Park SY, Shim SH, Kim H, Moon JH, Suh CS, Lim HJ. Human amnion-derived mesenchymal stem cells are a potential source for uterine stem cell therapy.. Cell Prolif 2008 Oct;41(5):709-25.
    pmc: PMC6496450pubmed: 18823496doi: 10.1111/j.1365-2184.2008.00553.xgoogle scholar: lookup
  51. Fischer C, Drillich M, Odau S, Heuwieser W, Einspanier R, Gabler C. Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis.. Reprod Fertil Dev 2010;22(5):818-29.
    pubmed: 20450834doi: 10.1071/rd09120google scholar: lookup
  52. Diedrich K, Fauser BC, Devroey P, Griesinger G. The role of the endometrium and embryo in human implantation.. Hum Reprod Update 2007 Jul-Aug;13(4):365-77.
    pubmed: 17548368doi: 10.1093/humupd/dmm011google scholar: lookup
  53. Chong AK, Chang J, Go JC. Mesenchymal stem cells and tendon healing.. Front Biosci (Landmark Ed) 2009 Jan 1;14(12):4598-605.
    pubmed: 19273374doi: 10.2741/3552google scholar: lookup
  54. Yagi H, Soto-Gutierrez A, Parekkadan B, Kitagawa Y, Tompkins RG, Kobayashi N, Yarmush ML. Mesenchymal stem cells: Mechanisms of immunomodulation and homing.. Cell Transplant 2010;19(6):667-79.
    pmc: PMC2957533pubmed: 20525442doi: 10.3727/096368910x508762google scholar: lookup

Citations

This article has been cited 18 times.
  1. Lange-Consiglio A, Gaspari G, Funghi F, Capra E, Cretich M, Frigerio R, Bosi G, Cremonesi F. Amniotic Mesenchymal-Derived Extracellular Vesicles and Their Role in the Prevention of Persistent Post-Breeding Induced Endometritis. Int J Mol Sci 2023 Mar 8;24(6).
    doi: 10.3390/ijms24065166pubmed: 36982240google scholar: lookup
  2. Iacono E, Merlo B. Stem Cells in Domestic Animals: Applications in Health and Production. Animals (Basel) 2022 Oct 13;12(20).
    doi: 10.3390/ani12202753pubmed: 36290139google scholar: lookup
  3. Iacono E, Lanci A, Gugole P, Merlo B. Shipping Temperature, Time and Media Effects on Equine Wharton's Jelly and Adipose Tissue Derived Mesenchymal Stromal Cells Characteristics. Animals (Basel) 2022 Aug 3;12(15).
    doi: 10.3390/ani12151967pubmed: 35953956google scholar: lookup
  4. Cequier A, Sanz C, Rodellar C, Barrachina L. The Usefulness of Mesenchymal Stem Cells beyond the Musculoskeletal System in Horses. Animals (Basel) 2021 Mar 25;11(4).
    doi: 10.3390/ani11040931pubmed: 33805967google scholar: lookup
  5. Rungsiwiwut R, Virutamasen P, Pruksananonda K. Mesenchymal stem cells for restoring endometrial function: An infertility perspective. Reprod Med Biol 2021 Jan;20(1):13-19.
    doi: 10.1002/rmb2.12339pubmed: 33488279google scholar: lookup
  6. Ambrósio CE, Orlandin JR, Oliveira VC, Motta LCB, Pinto PAF, Pereira VM, Padoveze LR, Karam RG, Pinheiro AO. Potential application of aminiotic stem cells in veterinary medicine. Anim Reprod 2020 May 22;16(1):24-30.
    doi: 10.21451/1984-3143-AR2018-00124pubmed: 33299475google scholar: lookup
  7. Lange-Consiglio A, Romele P, Magatti M, Silini A, Idda A, Martino NA, Cremonesi F, Parolini O. Priming with inflammatory cytokines is not a prerequisite to increase immune-suppressive effects and responsiveness of equine amniotic mesenchymal stromal cells. Stem Cell Res Ther 2020 Mar 4;11(1):99.
    doi: 10.1186/s13287-020-01611-zpubmed: 32131892google scholar: lookup
  8. Kim JH, Park M, Paek JY, Lee WS, Song H, Lyu SW. Intrauterine Infusion of Human Platelet-Rich Plasma Improves Endometrial Regeneration and Pregnancy Outcomes in a Murine Model of Asherman's Syndrome. Front Physiol 2020;11:105.
    doi: 10.3389/fphys.2020.00105pubmed: 32116803google scholar: lookup
  9. Pavathuparambil Abdul Manaph N, Sivanathan KN, Nitschke J, Zhou XF, Coates PT, Drogemuller CJ. An overview on small molecule-induced differentiation of mesenchymal stem cells into beta cells for diabetic therapy. Stem Cell Res Ther 2019 Sep 23;10(1):293.
    doi: 10.1186/s13287-019-1396-5pubmed: 31547868google scholar: lookup
  10. Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance. Cell Transplant 2018 Jan;27(1):93-116.
    doi: 10.1177/0963689717724797pubmed: 29562773google scholar: lookup
  11. Corradetti B, Taraballi F, Giretti I, Bauza G, Pistillo RS, Banche Niclot F, Pandolfi L, Demarchi D, Tasciotti E. Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes. Front Bioeng Biotechnol 2017;5:54.
    doi: 10.3389/fbioe.2017.00054pubmed: 28983481google scholar: lookup
  12. Corradetti B, Taraballi F, Martinez JO, Minardi S, Basu N, Bauza G, Evangelopoulos M, Powell S, Corbo C, Tasciotti E. Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation. Sci Rep 2017 Aug 11;7(1):7991.
    doi: 10.1038/s41598-017-08687-3pubmed: 28801676google scholar: lookup
  13. Hampton KK, Stewart R, Napier D, Claudio PP, Craven RJ. PGRMC1 Elevation in Multiple Cancers and Essential Role in Stem Cell Survival. Adv Lung Cancer (Irvine) 2015 Sep;4(3):37-51.
    doi: 10.4236/alc.2015.43006pubmed: 27867772google scholar: lookup
  14. Perrini C, Strillacci MG, Bagnato A, Esposti P, Marini MG, Corradetti B, Bizzaro D, Idda A, Ledda S, Capra E, Pizzi F, Lange-Consiglio A, Cremonesi F. Microvesicles secreted from equine amniotic-derived cells and their potential role in reducing inflammation in endometrial cells in an in-vitro model. Stem Cell Res Ther 2016 Nov 18;7(1):169.
    doi: 10.1186/s13287-016-0429-6pubmed: 27863532google scholar: lookup
  15. Fouad H, Sabry D, Elsetohy K, Fathy N. Therapeutic efficacy of amniotic membrane stem cells and adipose tissue stem cells in rats with chemically induced ovarian failure. J Adv Res 2016 Mar;7(2):233-41.
    doi: 10.1016/j.jare.2015.05.002pubmed: 26966564google scholar: lookup
  16. Fernandez-Moure JS, Corradetti B, Chan P, Van Eps JL, Janecek T, Rameshwar P, Weiner BK, Tasciotti E. Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis. Stem Cell Res Ther 2015 Oct 26;6:203.
    doi: 10.1186/s13287-015-0193-zpubmed: 26503337google scholar: lookup
  17. Vaiasicca S, James DW, Melone G, Saeed O, Francis LW, Corradetti B. Amniotic fluid-derived mesenchymal stem cells as a therapeutic tool against cytokine storm: a comparison with umbilical cord counterparts. Stem Cell Res Ther 2025 Mar 28;16(1):151.
    doi: 10.1186/s13287-025-04262-0pubmed: 40156072google scholar: lookup
  18. Maraldi T, Russo V. Amniotic Fluid and Placental Membranes as Sources of Stem Cells: Progress and Challenges 2.0. Int J Mol Sci 2023 Nov 7;24(22).
    doi: 10.3390/ijms242216020pubmed: 38003210google scholar: lookup
Subscribe to our newsletter
Join 99,911 horse owners like you who receive our email updates on horse health and nutrition.