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The Journal of antimicrobial chemotherapy2008; 62(3); 469-473; doi: 10.1093/jac/dkn233

Analysis and distribution of class 1 and class 2 integrons and associated gene cassettes among Escherichia coli isolates from swine, horses, cats and dogs collected in the BfT-GermVet monitoring study.

Abstract: In the BfT-GermVet monitoring study, 417 Escherichia coli isolates collected during 2004-06 in Germany from various disease conditions of pigs (n = 87), horses (n = 102) or cats/dogs (n = 228) were investigated for their susceptibility to 24 antimicrobial agents. This study dealt with the identification of integron-associated resistance genes among these isolates. Methods: Class 1 and class 2 integrons were detected by PCR. The variable parts of the integrons were cloned and sequenced. Transformation and conjugation experiments were conducted to confirm a plasmid location of the integrons. Results: Class 1 and/or class 2 integrons, alone or in different combinations, were detected in 79 of the 417 E. coli isolates. Four trimethoprim resistance genes (dfrA1/12/14/17), five streptomycin/spectinomycin resistance genes (aadA1/2/4/5/6), two streptothricin resistance genes (estX, sat2), one gentamicin/tobramycin/kanamycin resistance gene (aadB) and one chloramphenicol resistance gene (catB3) were detected. Seven different cassette arrangements were identified within class 1 integrons: aadA1 (21 isolates), dfrA1 + aadA1 (18 isolates), dfrA17 + aadA5 (9 isolates), dfrA12 + orfF + aadA2 (8 isolates), aadB + aadA1 (1 isolate), dfrA14 + recombined aadA6 (1 isolate) and dfrA1 + catB3 + aadA4 (1 isolate). Three different cassette arrangements in class 2 integrons, dfrA1 + sat2 + aadA1 (24 isolates), estX + sat2 + aadA1 (6 isolates) and estX + sat2 + DeltaaadA1 (1 isolate), were identified. The plasmid location of class 1 and/or class 2 integrons was confirmed in 37 isolates. Conclusions: Class 1 and/or class 2 integrons carrying resistance gene cassettes were detected in 18.9% of the isolates tested. This molecular analysis complements the phenotypic susceptibility testing conducted in the BfT-GermVet monitoring study and helps to explain the persistence of resistance genes even without direct selective pressure.
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Publication Date: 2008-06-11 PubMed ID: 18550679DOI: 10.1093/jac/dkn233Google Scholar: Lookup
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Summary

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The research article investigates the distribution and analysis of class 1 and class 2 integrons and associated gene cassettes among Escherichia coli isolates from swine, horses, cats and dogs collected during the BfT-GermVet monitoring study in Germany (2004-06). It uncovers how integrons – genetic components that contribute to antibiotic resistance – are distributed among these bacteria.

Objective and Methodology

  • The research primarily aimed to identify integron-associated resistance genes among 417 E. coli isolates obtained from diverse disease conditions of pigs, horses, cats, and dogs.
  • The researchers detected class 1 and class 2 integrons using Polymerase Chain Reaction (PCR). They cloned and sequenced the variable parts of these integrons.
  • To confirm a plasmid location of the integrons – genetic structures within a cell that can replicate independently – they conducted transformation and conjugation experiments.

Results

  • Among the 417 E. coli isolates, class 1 and/or class 2 integrons were detected in 79 cases, either alone or in different combinations.
  • Four trimethoprim resistance genes, five streptomycin/spectinomycin resistance genes, two streptothricin resistance genes, one gentamicin/tobramycin/kanamycin resistance gene, and one chloramphenicol resistance gene were identified.
  • Seven different cassette arrangements were found within class 1 integrons while three different cassette arrangements were discovered in class 2 integrons.
  • The plasmid location of class 1 and/or class 2 integrons was confirmed in 37 of the isolates.

Conclusions

  • The research revealed that 18.9% of the E. coli isolates carried class 1 and/or class 2 integrons housing resistance gene cassettes.
  • This molecular level analysis augments the phenotypic susceptibility testing conducted during the BfT-GermVet monitoring study.
  • It offered insights into how resistance genes persist, even without direct selective pressure, a vital finding in comprehending the dynamics of antibiotic resistance.

Cite This Article

APA
Kadlec K, Schwarz S. (2008). Analysis and distribution of class 1 and class 2 integrons and associated gene cassettes among Escherichia coli isolates from swine, horses, cats and dogs collected in the BfT-GermVet monitoring study. J Antimicrob Chemother, 62(3), 469-473. https://doi.org/10.1093/jac/dkn233

Publication

The Journal of antimicrobial chemotherapy
ISSN: 1460-2091
NlmUniqueID: 7513617
Country: England
Language: English
Volume: 62
Issue: 3
Pages: 469-473

Researcher Affiliations

Kadlec, Kristina
  • Institute of Farm Animal Genetics, Friedrich-Loeffler-Institute, Höltystr. 10, 31535 Neustadt-Mariensee, Germany.
Schwarz, Stefan

    MeSH Terms

    • Animals
    • Base Sequence
    • Cats
    • Chromosomes, Bacterial / genetics
    • Cloning, Molecular
    • DNA, Bacterial / genetics
    • Dogs
    • Drug Resistance, Bacterial
    • Escherichia coli / genetics
    • Escherichia coli / isolation & purification
    • Escherichia coli Infections / microbiology
    • Escherichia coli Infections / veterinary
    • Escherichia coli Proteins / genetics
    • Gene Order
    • Germany
    • Horses
    • Integrons
    • Molecular Sequence Data
    • Polymerase Chain Reaction
    • Sequence Alignment
    • Sequence Analysis, DNA
    • Swine

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