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Home / Equine Research Bank / J Virol / Analysis of equid herpesvirus 1 strain variation reveals a p...
Journal of virology2006; 80(8); 4047-4060; doi: 10.1128/JVI.80.8.4047-4060.2006

Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks.

Abstract: Equid herpesvirus 1 (EHV-1) can cause a wide spectrum of diseases ranging from inapparent respiratory infection to the induction of abortion and, in extreme cases, neurological disease resulting in paralysis and ultimately death. It has been suggested that distinct strains of EHV-1 that differ in pathogenic capacity circulate in the field. In order to investigate this hypothesis, it was necessary to identify genetic markers that allow subgroups of related strains to be identified. We have determined all of the genetic differences between a neuropathogenic strain (Ab4) and a nonneuropathogenic strain (V592) of EHV-1 and developed PCR/sequencing procedures enabling differentiation of EHV-1 strains circulating in the field. The results indicate the occurrence of several major genetic subgroups of EHV-1 among isolates recovered from outbreaks over the course of 30 years, consistent with the proposal that distinct strains of EHV-1 circulate in the field. Moreover, there is evidence that certain strain groups are geographically restricted, being recovered predominantly from outbreaks occurring in either North America or Europe. Significantly, variation of a single amino acid of the DNA polymerase is strongly associated with neurological versus nonneurological disease outbreaks. Strikingly, this variant amino acid occurs at a highly conserved position for herpesvirus DNA polymerases, suggesting an important functional role.
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Publication Date: 2006-03-31 PubMed ID: 16571821PubMed Central: PMC1440451DOI: 10.1128/JVI.80.8.4047-4060.2006Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the genetic differences in strains of equid herpesvirus 1 (EHV-1) and discovered a single DNA polymerase variation strongly linked to outbreaks of neurological versus nonneurological diseases.

Background and Objectives

  • EQUID herpesvirus 1 (EHV-1) is known for causing various diseases, including respiratory infections, abortion, paralysis and sometimes death in extreme conditions. The study sought to understand whether specific EHV-1 strains with different pathogenic capacities were present continuously.
  • The need to identify genetic indicators that could help identify related strain groups was crucial to this investigation.

Methodology

  • The study focused on the genetic differences between a neuropathogenic strain (Ab4) and a nonneuropathogenic strain (V592) of EHV-1.
  • PCR/sequencing procedures were developed to differentiate circulating EHV-1 strains.

Results and Discussion

  • The study found several major genetic subgroups of EHV-1 among isolates collected from outbreaks over a 30-year span. This finding was consistent with the hypothesis that distinct EHV-1 strains are continually present in the field.
  • It was also discovered that certain strain groups were geographically bound, being predominantly found in North America or Europe.
  • Notably, the single amino acid variation in the DNA polymerase was firmly linked to neurological versus nonneurological disease outbreaks. The role of this variant was emphasized due to its position at a highly conserved location for herpesvirus DNA polymerases.

Implications

  • The study has significant implications for understanding the nature of EHV-1 strains and their distinct impacts, potentially paving the way for more effective and precise responses to different EHV-1 outbreak types in the future.
  • The specific DNA polymerase variation identified could serve as a vital marker for predicting the nature of disease outbreaks, whether neurological or nonneurological.

Cite This Article

APA
Nugent J, Birch-Machin I, Smith KC, Mumford JA, Swann Z, Newton JR, Bowden RJ, Allen GP, Davis-Poynter N. (2006). Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks. J Virol, 80(8), 4047-4060. https://doi.org/10.1128/JVI.80.8.4047-4060.2006

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 80
Issue: 8
Pages: 4047-4060

Researcher Affiliations

Nugent, J
  • Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, United Kingdom.
Birch-Machin, I
    Smith, K C
      Mumford, J A
        Swann, Z
          Newton, J R
            Bowden, R J
              Allen, G P
                Davis-Poynter, N

                  MeSH Terms

                  • Amino Acid Sequence
                  • Animals
                  • Base Sequence
                  • DNA-Directed DNA Polymerase / chemistry
                  • DNA-Directed DNA Polymerase / genetics
                  • Disease Outbreaks
                  • Herpesvirus 1, Equid / classification
                  • Herpesvirus 1, Equid / genetics
                  • Herpesvirus 1, Equid / pathogenicity
                  • Horse Diseases / epidemiology
                  • Horses
                  • Molecular Sequence Data
                  • Open Reading Frames
                  • Point Mutation
                  • Polymorphism, Single Nucleotide

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