Antibacterial effect of acidic ionized water on horse wounds bacterial isolates.
Abstract: Horse wounds can be easily infected with bacteria depending on the nature of its cause such as laceration, abrasion, or puncture as well as the nature of its environment. Various treatments are available in managing open wounds, including the usage of topical antibiotics and antiseptics. However, antibiotic resistance has been a major concern attributed with chronic wound infection. The aim of this study was to test the efficacy of ionized water at different pH against the growth of common bacteria from horse wounds. Methods: Ten swab samples from equine infected wounds were collected and bacteria isolation and identification were performed. The antibacterial effect of the ionized water of pH 2.5, 4.5, 7.0, and 11.5 was tested on Staphylococcus aureus, Staphylococcus pseudintermedius, Staphylococcus intermedius, Escherichia coli, Pantoea agglomerans, and Klebsiella pneumoniae. The time-kill profiles of the ionized waters were determined at time 0, 2, 4, 6, and 8 h. Results: Ionized water of pH 2.5 and 4.5 showed antibacterial activity against S. aureus, S. pseudintermedius, and S. intermedius with significant (p>0.05) reduction in colony-forming unit/mL within 2-8 h. The degree of bactericidal effect of the acidic ionized water differs between the species with S. intermedius more susceptible. However, there was no antibacterial effect at pH 2.5, 4.5, 7.0, and 11.5 on the Gram-negative bacteria tested. Conclusions: Ionized water of pH 2.5 and 4.5 is effective in minimizing the growth of Gram-positive bacteria; thus it could be of clinical importance as an antiseptic for surface wound lavage in horses.
Copyright: © Rahman, et al.
Publication Date: 2021-05-10 PubMed ID: 34220113PubMed Central: PMC8243685DOI: 10.14202/vetworld.2021.1128-1132Google Scholar: Lookup
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- Journal Article
Summary
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The study examines the effectiveness of acidic ionized water in preventing bacterial growth in horse wounds. The research suggests that ionized water of certain pH levels can inhibit growth of specific bacteria, thus potentially serving as an important antiseptic in treating horse wounds.
Study Methods and Procedures
- The research methodology involved the collection of ten swab samples from infected equine wounds. Following this, isolation and identification of bacteria present on these samples were performed.
- The researchers tested the antibacterial effects of ionized water at four different pH levels — 2.5, 4.5, 7.0, and 11.5. The experiment was focused on detecting any bactericidal effect on common bacteria found in horse wounds
- To assess the bacterial behavior in contact with ionized water, time-kill profiles were determined at various intervals — 0, 2, 4, 6, and 8 hours.
Study Results
- The results indicated that ionized water with a pH of 2.5 and 4.5 demonstrated antimicrobial activity against certain types of Gram-positive bacteria. A significant reduction in colony-forming units per milliliter was observed within 2 to 8 hours of exposure.
- The degree of bactericidal effect varied among species, with one particular bacteria found to be more susceptible.
- However, the study showed that none of the ionized water pH levels had an antibacterial effect on any of the Gram-negative bacteria tested.
Study Conclusions
- The study concluded that ionized water with a pH of 2.5 and 4.5 showed effectiveness in inhibiting the growth of Gram-positive bacteria. Considering these findings, it is suggested that such water could potentially be utilized as an antiseptic for surface wound cleaning in horses.
- However, the research found no significant impact on the growth of Gram-negative bacteria indicating that this method might not be universally applicable for all types of bacterial infections in horse wounds.
- This study lays the groundwork for further exploration and development of new antiseptic solutions to combat infections in horse wounds, especially considering the increasing problem of antibiotic resistance.
Cite This Article
APA
Rahman AZA, Adzahan NM, Zakaria Z, Mayaki AM.
(2021).
Antibacterial effect of acidic ionized water on horse wounds bacterial isolates.
Vet World, 14(5), 1128-1132.
https://doi.org/10.14202/vetworld.2021.1128-1132 Publication
Researcher Affiliations
- Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
- Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
- Department of Veterinary Medicine, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, PMB2346, City Campus, Sokoto, Nigeria.
- Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
- Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
- Department of Veterinary Medicine, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, PMB2346, City Campus, Sokoto, Nigeria.
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Citations
This article has been cited 1 times.- Darwish RM, Salama AH. Developing antibacterial peptides as a promising therapy for combating antibiotic-resistant Pseudomonas aeruginosa infections. Vet World 2024 Jun;17(6):1259-1264.
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