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Home / Equine Research Bank / Vet Surg / Antibacterial properties of a silver chloride-coated nylon w...
Veterinary surgery : VS1999; 28(4); 219-225; doi: 10.1053/jvet.1999.0219

Antibacterial properties of a silver chloride-coated nylon wound dressing.

Abstract: A silver chloride-coated nylon wound dressing (Ag-WD) was evaluated in vitro for antimicrobial activity against five common equine wound pathogens. Methods: Bacterial susceptibility study. Methods: Equine wound pathogens: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus equi subspecies zooepidemicus, and Staphylococcus aureus. Methods: An inoculum of each pathogen was incubated directly with Ag-WD and quantitated after 24 to 48 hours of incubation. To determine if bactericidal activity of Ag-WD was contact dependent, an inoculum of E. coli and Staphylococcus aureus was incubated separately from Ag-WD by a filter and quantitated after 18 hours of incubation. Inductively coupled plasma emission spectrometry (ICP) determined the silver concentration of Mueller-Hinton broth containing Ag-WD after 24 hours of incubation. To establish if the rate of bacterial killing by Ag-WD differed from a constant silver concentration, pathogens were exposed to a silver concentration of 6.45 microg/mL and quantitated after 18 hours. Results: Direct exposure to Ag-WD significantly reduced bacterial numbers after 15 minutes for K. pneumoniae, 30 minutes for E. coli, 1 hour for P. aeruginosa, and 2 hours for S. equi subspecies zooepidemicus and Staphylococcus aureus. Indirect exposure to Ag-WD resulted in > or =99.9% and > or =90% kill of the inoculum doses of E. coli at 2 hours and Staphylococcus aureus at 18 hours, respectively. Incubation of the pathogens at the constant silver concentration resulted in bacterial killing rates similar to those obtained by incubation with Ag-WD. Conclusions: In vitro, equine pathogens are effectively killed when exposed to Ag-WD, and the rate of bacterial killing by Ag-WD is similar to a constant silver concentration of 6.45 microg/mL. Conclusions: The in vitro antimicrobial properties of this silver-coated nylon wound dressing are promising for future prevention of equine wound infections.
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Publication Date: 1999-07-29 PubMed ID: 10424701DOI: 10.1053/jvet.1999.0219Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the antibacterial properties of a silver-coated nylon wound dressing. The study reveals that this dressing effectively kills equine pathogens, showing promise for preventing infections in horse wounds.

Research Methods

  • The research primarily focused on testing the antimicrobial activity of a silver chloride-coated nylon wound dressing (Ag-WD) against five common equine wound pathogens – Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus equi subspecies zooepidemicus, and Staphylococcus aureus.
  • An inoculum of each of these pathogens was directly incubated with the Ag-WD and later quantified after a period of 24 to 48 hours.
  • To verify if the bactericidal activity of Ag-WD was contact dependent, separate tests were run with E. coli and Staphylococcus aureus where they were kept apart from the Ag-WD through a filter. The bacterial quantities were again monitored after an 18 hour incubation.
  • Inductively coupled plasma emission spectrometry (ICP) was employed to establish the silver concentration in the broth containing Ag-WD after 24 hours of incubation.
  • Finally, to understand if the Ag-WD kills bacteria differently from a constant silver concentration, the pathogens were exposed to a silver concentration of 6.45 microg/mL and their numbers were quantified after 18 hours.

Research Results

  • The results revealed that the pathogens’ direct exposure to Ag-WD diminished bacterial counts significantly – after 15 minutes for K. pneumoniae, 30 minutes for E. coli, 1 hour for P. aeruginosa, and 2 hours for S. equi subspecies zooepidemicus and Staphylococcus aureus.
  • Even with indirect exposure, the Ag-WD had considerable lethal effects, causing a drop of 99.9% and 90% of the initial E. coli and Staphylococcus aureus quantities respectively after the designated time periods.
  • Exposing pathogens to a constant silver concentration resulted in bacterial killing rates similar to those obtained by incubation with Ag-WD.

Conclusion

  • This study demonstrates that the Ag-WD effectively kills equine pathogens in vitro. Note that the bacterial killing rate by Ag-WD is comparable to a constant silver concentration of 6.45 microg/mL.
  • Given these results, the research concludes that the antimicrobial properties of the silver-coated nylon wound dressing are promising for future use in preventing infections in equine wounds.

Cite This Article

APA
Adams AP, Santschi EM, Mellencamp MA. (1999). Antibacterial properties of a silver chloride-coated nylon wound dressing. Vet Surg, 28(4), 219-225. https://doi.org/10.1053/jvet.1999.0219

Publication

Veterinary surgery : VS
ISSN: 0161-3499
NlmUniqueID: 8113214
Country: United States
Language: English
Volume: 28
Issue: 4
Pages: 219-225

Researcher Affiliations

Adams, A P
  • Department of Clinical and Population Sciences, University of Minnesota, College of Veterinary Medicine, St. Paul, USA.
Santschi, E M
    Mellencamp, M A

      MeSH Terms

      • Animals
      • Anti-Bacterial Agents / pharmacology
      • Bacteria / drug effects
      • Bandages / veterinary
      • Horses / injuries
      • Horses / surgery
      • Microbial Sensitivity Tests / veterinary
      • Nylons
      • Silver Compounds / pharmacology
      • Surgical Wound Infection / prevention & control
      • Surgical Wound Infection / veterinary
      • Wound Healing

      Citations

      This article has been cited 13 times.
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