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PloS one2018; 13(8); e0201691; doi: 10.1371/journal.pone.0201691

Anticipatory response before competition in Standardbred racehorses.

Abstract: It is generally accepted that besides cortisol concentrations, parameters of heart rate variability (HRV) are appropriate indicators of stress in horses. The aim of this study was to determine anticipatory stress in eight Standardbred stallions participating in harness race. Cortisol and HRV responses to a mild exercise performed in training circumstances were compared to a maximal effort exercise performed in real trotting race conditions. Parameters of HRV reflecting vagal (root mean square of the successive differences, RMSSD) and sympathetic nervous system activity (ratio of the low and high frequency component, LF/HF) were recorded before warming up (baseline) and during exercise. Plasma cortisol concentrations were obtained for the following stages of the exercise: before warming up (baseline), after warming up, after the exercise has finished and after a 30-min recovery. Baseline LF/HF ratio was higher before the race compared to the pre-training values (12.0 ± 6.6 vs. 5.9 ± 4.5, P = 0.009), while RMSSD did not show such difference (34.8 ± 15.9 ms vs. 48.0 ± 30.5 ms, P = 0.96). Cortisol level was higher in the case of race for all samples compared to training (P = 0.012). There were no significant differences between plasma cortisol levels obtained for the subsequent stages of race. Horses in the present study showed anticipatory response before race as shown by differences in pre-training (97.3 ± 16.4 nmol/L) and pre-race cortisol levels (171.8 ± 18.7 nmol/L), respectively (P < 0.001). Pre-race HRV only partly confirmed this phenomenon.
Publication Date: 2018-08-02 PubMed ID: 30071079PubMed Central: PMC6072081DOI: 10.1371/journal.pone.0201691Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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This research investigates potential stress responses in Standardbred stallions that are being prepared for a harness race. The study’s objective was to measure concentrations of cortisol and the parameters of heart rate variability (HRV) before and during the race, and compare them to those found in the horses during training. The findings reveal that there is a significant anticipation response, evidenced by varying cortisol and HRV levels in the horses.

Research Background and Aim

  • The study is established on the premise that parameters of heart rate variability (HRV) and cortisol concentrations are appropriate stress indicators in horses.
  • The research sought to determine if there are anticipatory stress responses in eight Standardbred stallions that were preparing for harness races.

Research Methodology

  • During training and during the actual horse races, the researchers measured cortisol and HRV responses.
  • The HRV measurements recorded both sympathetic nervous system activity (using the ratio of low to high frequency component, abbreviated as LF/HF) and vagal activity (measured using the root mean square of successive differences, abbreviated as RMSSD).
  • They compared different stages including before warm up (baseline), after warming up, after the exercise, and after a 30-minutes recovery.
  • The researchers therefore sought to establish if there were significant differences between the races and the training sessions.
  • The research also aimed at determining if there were differences between subsequent stages of the races.

Research Findings

  • It was observed that both the LF/HF ratio and cortisol levels were higher before the race compared to the pre-training values.
  • However, the RMSSD did not show a significant difference before the race and during training.
  • Interestingly, there were no significant differences between the levels of plasma cortisol recorded at the various stages of the race.
  • Despite these findings, it is concluded that the horses did show signs of anticipatory response before the race because the pre-race cortisol levels were significantly higher than the pre-training levels.
  • This demonstrates that racing conditions create physiological stress responses in horses that are not seen during regular training, thus emphasizing the importance of understanding stress responses for maintaining optimal horse health and performance.

Cite This Article

APA
Bohák Z, Harnos A, Joó K, Szenci O, Kovács L. (2018). Anticipatory response before competition in Standardbred racehorses. PLoS One, 13(8), e0201691. https://doi.org/10.1371/journal.pone.0201691

Publication

ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 13
Issue: 8
Pages: e0201691
PII: e0201691

Researcher Affiliations

Bohák, Zsófia
  • MTA-SZIE Large Animal Clinical Research Group, Üllő, Dóra major, Hungary.
Harnos, Andrea
  • Department of Biomathematics and Informatics, University of Veterinary Medicine, Budapest, Hungary.
Joó, Kinga
  • MTA-SZIE Large Animal Clinical Research Group, Üllő, Dóra major, Hungary.
Szenci, Ottó
  • MTA-SZIE Large Animal Clinical Research Group, Üllő, Dóra major, Hungary.
Kovács, Levente
  • MTA-SZIE Large Animal Clinical Research Group, Üllő, Dóra major, Hungary.
  • Institute of Animal Husbandry, Faculty of Agricultural and Environmental Science, Szent István University, Gödöllő, Hungary.

MeSH Terms

  • Animals
  • Anticipation, Psychological
  • Heart Rate
  • Horses
  • Hydrocortisone / blood
  • Sports / physiology
  • Stress, Psychological / blood
  • Stress, Psychological / physiopathology
  • Stress, Psychological / psychology

Conflict of Interest Statement

The authors have declared that no competing interests exist.

References

This article includes 26 references

Citations

This article has been cited 6 times.
  1. Olvera-Maneu S, Carbajal A, Serres-Corral P, López-Béjar M. Cortisol Variations to Estimate the Physiological Stress Response in Horses at a Traditional Equestrian Event.. Animals (Basel) 2023 Jan 24;13(3).
    doi: 10.3390/ani13030396pubmed: 36766285google scholar: lookup
  2. Schrurs C, Dubois G, Van Erck-Westergren E, Gardner DS. Does sex of the jockey influence racehorse physiology and performance.. PLoS One 2022;17(8):e0273310.
    doi: 10.1371/journal.pone.0273310pubmed: 36044425google scholar: lookup
  3. Nyerges-Bohák Z, Nagy K, Rózsa L, Póti P, Kovács L. Heart rate variability before and after 14 weeks of training in Thoroughbred horses and Standardbred trotters with different training experience.. PLoS One 2021;16(12):e0259933.
    doi: 10.1371/journal.pone.0259933pubmed: 34882704google scholar: lookup
  4. Tuomola K, Mäki-Kihniä N, Valros A, Mykkänen A, Kujala-Wirth M. Risk factors for bit-related lesions in Finnish trotting horses.. Equine Vet J 2021 Nov;53(6):1132-1140.
    doi: 10.1111/evj.13401pubmed: 33336423google scholar: lookup
  5. Lopedote M, Valentini S, Musella V, Vilar JM, Spinella G. Changes in Pulse Rate, Respiratory Rate and Rectal Temperature in Working Dogs before and after Three Different Field Trials.. Animals (Basel) 2020 Apr 23;10(4).
    doi: 10.3390/ani10040733pubmed: 32340191google scholar: lookup
  6. Bohák Z, Harnos A, Joó K, Szenci O, Kovács L. Correction: Anticipatory response before competition in Standardbred racehorses.. PLoS One 2018;13(11):e0208521.
    doi: 10.1371/journal.pone.0208521pubmed: 30496277google scholar: lookup