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Home / Equine Research Bank / Vet Microbiol / Antigenic and genetic variations in European and North Ameri...
Veterinary microbiology2009; 138(1-2); 41-52; doi: 10.1016/j.vetmic.2009.03.004

Antigenic and genetic variations in European and North American equine influenza virus strains (H3N8) isolated from 2006 to 2007.

Abstract: Equine influenza virus (EIV) surveillance is important in the management of equine influenza. It provides data on circulating and newly emerging strains for vaccine strain selection. To this end, antigenic characterisation by haemaggluttination inhibition (HI) assay and phylogenetic analysis was carried out on 28 EIV strains isolated in North America and Europe during 2006 and 2007. In the UK, 20 viruses were isolated from 28 nasopharyngeal swabs that tested positive by enzyme-linked immunosorbent assay. All except two of the UK viruses were characterised as members of the Florida sublineage with similarity to A/eq/Newmarket/5/03 (clade 2). One isolate, A/eq/Cheshire/1/06, was characterised as an American lineage strain similar to viruses isolated up to 10 years earlier. A second isolate, A/eq/Lincolnshire/1/07 was characterised as a member of the Florida sublineage (clade 1) with similarity to A/eq/Wisconsin/03. Furthermore, A/eq/Lincolnshire/1/06 was a member of the Florida sublineage (clade 2) by haemagglutinin (HA) gene sequence, but appeared to be a member of the Eurasian lineage by the non-structural gene (NS) sequence suggesting that reassortment had occurred. A/eq/Switzerland/P112/07 was characterised as a member of the Eurasian lineage, the first time since 2005 that isolation of a virus from this lineage has been reported. Seven viruses from North America were classified as members of the Florida sublineage (clade 1), similar to A/eq/Wisconsin/03. In conclusion, a variety of antigenically distinct EIVs continue to circulate worldwide. Florida sublineage clade 1 viruses appear to predominate in North America, clade 2 viruses in Europe.
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Publication Date: 2009-03-13 PubMed ID: 19346084DOI: 10.1016/j.vetmic.2009.03.004Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study analyzes the differences in strains of equine influenza virus (EIV) found in Europe and North America between 2006 and 2007. The result indicates that a variety of distinct EIVs circulate globally, with specific strains predominant in North America and Europe.

About the Research

This research was conducted as a part of equine influenza virus (EIV) surveillance, crucial for managing equine influenza. It helps gather data regarding circulating and newly emerging strains for effective selection of vaccine strains.

  • The researchers conducted antigenic characterization and phylogenetic analysis on 28 EIV strains that were isolated in North America and Europe during 2006 and 2007.
  • They used haemagglutination inhibition (HI) assay for the antigenic characterization, which is a primary method used to identify and classify serotypes of virus.
  • The phylogenetic analysis was essential for tracing the evolution of these strains.

Findings

Based on the analysis, researchers made several conclusions regarding the origins and characteristics of the EIV strains isolated during the study.

  • In the UK, 20 viruses out of 28 nasopharyngeal swabs that were positive in an enzyme-linked immunosorbent assay were isolated.
  • Except for two, all UK viruses were characterized as members of the Florida sublineage. They had similarities to A/eq/Newmarket/5/03, a strain classified under clade 2.
  • An isolate named A/eq/Cheshire/1/06 came out as an American lineage strain, akin to viruses isolated up to a decade earlier.
  • A second isolate named A/eq/Lincolnshire/1/07 was featured as a member of the Florida sublineage (clade 1) with a similarity to A/eq/Wisconsin/03.
  • Another A/eq/Lincolnshire/1/06 was a member of the Florida sublineage (clade 2) by the haemagglutinin (HA) gene sequence. However, revealed a connection to the Eurasian lineage by the non-structural gene (NS) sequence. This suggests that reassortment had occurred.
  • Virus A/eq/Switzerland/P112/07 was characterized as a member of the Eurasian lineage. This was significant as it represented the first time since 2005 that the isolation of a virus from this lineage was reported.
  • Seven viruses from North America were classified as members of the Florida sublineage (clade 1), akin to A/eq/Wisconsin/03.

Conclusion

The research concludes that a wide variety of antigenically distinct EIVs continue to circulate worldwide. Florida sublineage clade 1 viruses appear to be more common in North America, and clade 2 viruses more common in Europe, thus showing a difference in the prevalence of EIV strains between the two regions.

Cite This Article

APA
Bryant NA, Rash AS, Russell CA, Ross J, Cooke A, Bowman S, MacRae S, Lewis NS, Paillot R, Zanoni R, Meier H, Griffiths LA, Daly JM, Tiwari A, Chambers TM, Newton JR, Elton DM. (2009). Antigenic and genetic variations in European and North American equine influenza virus strains (H3N8) isolated from 2006 to 2007. Vet Microbiol, 138(1-2), 41-52. https://doi.org/10.1016/j.vetmic.2009.03.004

Publication

Veterinary microbiology
ISSN: 1873-2542
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 138
Issue: 1-2
Pages: 41-52

Researcher Affiliations

Bryant, Neil A
  • Animal Health Trust, Centre for Preventive Medicine, Lanwades Park, Kentford, Newmarket CB8 7UU, UK. neil.bryant@aht.org.uk
Rash, Adam S
    Russell, Colin A
      Ross, Julie
        Cooke, Annie
          Bowman, Samantha
            MacRae, Shona
              Lewis, Nicola S
                Paillot, Romain
                  Zanoni, Reto
                    Meier, Hanspeter
                      Griffiths, Lowri A
                        Daly, Janet M
                          Tiwari, Ashish
                            Chambers, Thomas M
                              Newton, J Richard
                                Elton, Debra M

                                  MeSH Terms

                                  • Amino Acid Sequence
                                  • Animals
                                  • Antibodies, Viral / immunology
                                  • Chickens / virology
                                  • Enzyme-Linked Immunosorbent Assay
                                  • Erythrocytes / virology
                                  • Europe
                                  • Genes, Viral
                                  • Genetic Variation
                                  • Horse Diseases / virology
                                  • Horses
                                  • Influenza A Virus, H3N8 Subtype / classification
                                  • Influenza A Virus, H3N8 Subtype / genetics
                                  • Influenza A Virus, H3N8 Subtype / isolation & purification
                                  • Lung Diseases / veterinary
                                  • Lung Diseases / virology
                                  • Nasopharynx / virology
                                  • North America
                                  • Orthomyxoviridae Infections / veterinary
                                  • Polymerase Chain Reaction
                                  • Sequence Alignment
                                  • Viral Nonstructural Proteins / genetics

                                  Grant Funding

                                  • DP1-OD000490-01 / NIH HHS

                                  Citations

                                  This article has been cited 52 times.
                                  1. Wasik BR, Rothschild E, Voorhees IEH, Reedy SE, Murcia PR, Pusterla N, Chambers TM, Goodman LB, Holmes EC, Kile JC, Parrish CR. Understanding the divergent evolution and epidemiology of H3N8 influenza viruses in dogs and horses.. Virus Evol 2023;9(2):vead052.
                                    doi: 10.1093/ve/vead052pubmed: 37692894google scholar: lookup
                                  2. Pellegrini F, Buonavoglia A, Omar AH, Diakoudi G, Lucente MS, Odigie AE, Sposato A, Augelli R, Camero M, Decaro N, Elia G, Bányai K, Martella V, Lanave G. A Cold Case of Equine Influenza Disentangled with Nanopore Sequencing.. Animals (Basel) 2023 Mar 24;13(7).
                                    doi: 10.3390/ani13071153pubmed: 37048408google scholar: lookup
                                  3. Olufemi OT, Edeh ER, Isyaku MS, Haliru M, Samaila S, Mshelia PW, Owolodun OA, Newton JR, Daly JM. Seroprevalence of Equine Influenza and Its Associated Risk Factors in Northwest Nigeria.. Pathogens 2022 Nov 17;11(11).
                                    doi: 10.3390/pathogens11111372pubmed: 36422623google scholar: lookup
                                  4. Ahmed BM, Bayoumi MM, Farrag MA, Elgamal MA, Daly JM, Amer HM. Emergence of equine influenza virus H3Nx Florida clade 2 in Arabian racehorses in Egypt.. Virol J 2022 Nov 12;19(1):185.
                                    doi: 10.1186/s12985-022-01917-9pubmed: 36371185google scholar: lookup
                                  5. Alaql FA, Alhafufi AN, Kasem S, Alhammad YMO, Albaqshi H, Alyousaf A, Alsubaie FM, Alghamdi AN, Abdel-Moneim AS, Alharbi SA. Full-Length Genome of the Equine Influenza A Virus Subtype H3N8 from 2019 Outbreak in Saudi Arabia.. Animals (Basel) 2022 Oct 10;12(19).
                                    doi: 10.3390/ani12192720pubmed: 36230462google scholar: lookup
                                  6. Whitlock F, Grewar J, Newton R. An epidemiological overview of the equine influenza epidemic in Great Britain during 2019.. Equine Vet J 2023 Jan;55(1):153-164.
                                    doi: 10.1111/evj.13874pubmed: 36054725google scholar: lookup
                                  7. Whitlock F, Murcia PR, Newton JR. A Review on Equine Influenza from a Human Influenza Perspective.. Viruses 2022 Jun 15;14(6).
                                    doi: 10.3390/v14061312pubmed: 35746783google scholar: lookup
                                  8. El-Hage C, Hartley C, Savage C, Watson J, Gilkerson J, Paillot R. Assessment of Humoral and Long-Term Cell-Mediated Immune Responses to Recombinant Canarypox-Vectored Equine Influenza Virus Vaccination in Horses Using Conventional and Accelerated Regimens Respectively.. Vaccines (Basel) 2022 May 26;10(6).
                                    doi: 10.3390/vaccines10060855pubmed: 35746463google scholar: lookup
                                  9. Oladunni FS, Oseni SO, Martinez-Sobrido L, Chambers TM. Equine Influenza Virus and Vaccines.. Viruses 2021 Aug 20;13(8).
                                    doi: 10.3390/v13081657pubmed: 34452521google scholar: lookup
                                  10. Alnaeem A, Shawaf T, Ali AM, Hemida MG. Clinical observations and molecular detection of Type-A influenza virus in some of the family Equidae in eastern Saudi Arabia winter-2019.. Vet Res Commun 2021 Dec;45(4):423-430.
                                    doi: 10.1007/s11259-021-09822-2pubmed: 34435308google scholar: lookup
                                  11. Daly JM, Megid J, Langoni H, de Nardi Júnior G, Ribeiro MG. Retrospective serological survey for influenza in horses from Brazil.. Braz J Microbiol 2021 Mar;52(1):461-466.
                                    doi: 10.1007/s42770-020-00398-8pubmed: 33175343google scholar: lookup
                                  12. Blanco-Lobo P, Rodriguez L, Reedy S, Oladunni FS, Nogales A, Murcia PR, Chambers TM, Martinez-Sobrido L. A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza Virus.. Viruses 2019 Oct 11;11(10).
                                    doi: 10.3390/v11100933pubmed: 31614538google scholar: lookup
                                  13. Gahan J, Garvey M, Asmah Abd Samad R, Cullinane A. Whole Genome Sequencing of the First H3N8 Equine Influenza Virus Identified in Malaysia.. Pathogens 2019 May 10;8(2).
                                    doi: 10.3390/pathogens8020062pubmed: 31083430google scholar: lookup
                                  14. Toh X, Soh ML, Ng MK, Yap SC, Harith N, Fernandez CJ, Huangfu T. Isolation and characterization of equine influenza virus (H3N8) from an equine influenza outbreak in Malaysia in 2015.. Transbound Emerg Dis 2019 Sep;66(5):1884-1893.
                                    doi: 10.1111/tbed.13218pubmed: 31059176google scholar: lookup
                                  15. Kwaśnik M, Góra IM, Żmudziński JF, Rola J, Polak MP, Rożek W. Genetic Analysis of the M Gene of Equine Influenza Virus Strains Isolated in Poland, in the Context of the Asian-like Group Formation.. J Vet Res 2018 Dec;62(4):405-412.
                                    doi: 10.2478/jvetres-2018-0057pubmed: 30729195google scholar: lookup
                                  16. Singh RK, Dhama K, Karthik K, Khandia R, Munjal A, Khurana SK, Chakraborty S, Malik YS, Virmani N, Singh R, Tripathi BN, Munir M, van der Kolk JH. A Comprehensive Review on Equine Influenza Virus: Etiology, Epidemiology, Pathobiology, Advances in Developing Diagnostics, Vaccines, and Control Strategies.. Front Microbiol 2018;9:1941.
                                    doi: 10.3389/fmicb.2018.01941pubmed: 30237788google scholar: lookup
                                  17. Gildea S, Garvey M, Lyons P, Lyons R, Gahan J, Walsh C, Cullinane A. Multifocal Equine Influenza Outbreak with Vaccination Breakdown in Thoroughbred Racehorses.. Pathogens 2018 Apr 17;7(2).
                                    doi: 10.3390/pathogens7020043pubmed: 29673169google scholar: lookup
                                  18. Rodriguez L, Reedy S, Nogales A, Murcia PR, Chambers TM, Martinez-Sobrido L. Development of a novel equine influenza virus live-attenuated vaccine.. Virology 2018 Mar;516:76-85.
                                    doi: 10.1016/j.virol.2018.01.005pubmed: 29331866google scholar: lookup
                                  19. Sreenivasan CC, Jandhyala SS, Luo S, Hause BM, Thomas M, Knudsen DEB, Leslie-Steen P, Clement T, Reedy SE, Chambers TM, Christopher-Hennings J, Nelson E, Wang D, Kaushik RS, Li F. Phylogenetic Analysis and Characterization of a Sporadic Isolate of Equine Influenza A H3N8 from an Unvaccinated Horse in 2015.. Viruses 2018 Jan 11;10(1).
                                    doi: 10.3390/v10010031pubmed: 29324680google scholar: lookup
                                  20. Favaro PF, Fernandes WR, Reischak D, Brandão PE, Silva SOS, Richtzenhain LJ. Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015.. Braz J Microbiol 2018 Apr-Jun;49(2):336-346.
                                    doi: 10.1016/j.bjm.2017.07.003pubmed: 29100932google scholar: lookup
                                  21. Gahan J, Garvey M, Gildea S, Gür E, Kagankaya A, Cullinane A. Whole-genome sequencing and antigenic analysis of the first equine influenza virus identified in Turkey.. Influenza Other Respir Viruses 2018 May;12(3):374-382.
                                    doi: 10.1111/irv.12485pubmed: 28940727google scholar: lookup
                                  22. Bera BC, Virmani N, Kumar N, Anand T, Pavulraj S, Rash A, Elton D, Rash N, Bhatia S, Sood R, Singh RK, Tripathi BN. Genetic and codon usage bias analyses of polymerase genes of equine influenza virus and its relation to evolution.. BMC Genomics 2017 Aug 23;18(1):652.
                                    doi: 10.1186/s12864-017-4063-1pubmed: 28830350google scholar: lookup
                                  23. Rash A, Morton R, Woodward A, Maes O, McCauley J, Bryant N, Elton D. Evolution and Divergence of H3N8 Equine Influenza Viruses Circulating in the United Kingdom from 2013 to 2015.. Pathogens 2017 Feb 8;6(1).
                                    doi: 10.3390/pathogens6010006pubmed: 28208721google scholar: lookup
                                  24. Olguin Perglione C, Golemba MD, Torres C, Barrandeguy M. Molecular Epidemiology and Spatio-Temporal Dynamics of the H3N8 Equine Influenza Virus in South America.. Pathogens 2016 Oct 16;5(4).
                                    doi: 10.3390/pathogens5040061pubmed: 27754468google scholar: lookup
                                  25. Barba M, Daly JM. The Influenza NS1 Protein: What Do We Know in Equine Influenza Virus Pathogenesis?. Pathogens 2016 Aug 31;5(3).
                                    doi: 10.3390/pathogens5030057pubmed: 27589809google scholar: lookup
                                  26. Alves Beuttemmüller E, Woodward A, Rash A, Dos Santos Ferraz LE, Fernandes Alfieri A, Alfieri AA, Elton D. Characterisation of the epidemic strain of H3N8 equine influenza virus responsible for outbreaks in South America in 2012.. Virol J 2016 Mar 19;13:45.
                                    doi: 10.1186/s12985-016-0503-9pubmed: 26993620google scholar: lookup
                                  27. Perglione CO, Gildea S, Rimondi A, Miño S, Vissani A, Carossino M, Cullinane A, Barrandeguy M. Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012.. Influenza Other Respir Viruses 2016 Jan;10(1):37-46.
                                    doi: 10.1111/irv.12349pubmed: 26406274google scholar: lookup
                                  28. Paillot R. A Systematic Review of Recent Advances in Equine Influenza Vaccination.. Vaccines (Basel) 2014 Nov 14;2(4):797-831.
                                    doi: 10.3390/vaccines2040797pubmed: 26344892google scholar: lookup
                                  29. Kim EJ, Kim BH, Yang S, Choi EJ, Shin YJ, Song JY, Shin YK. Antibody responses after vaccination against equine influenza in the Republic of Korea in 2013.. J Vet Med Sci 2015 Nov;77(11):1517-21.
                                    doi: 10.1292/jvms.15-0192pubmed: 26062436google scholar: lookup
                                  30. Su S, Wang L, Fu X, He S, Hong M, Zhou P, Lai A, Gray G, Li S. Equine influenza A(H3N8) virus infection in cats.. Emerg Infect Dis 2014 Dec;20(12):2096-9.
                                    doi: 10.3201/eid2012.140867pubmed: 25417790google scholar: lookup
                                  31. Rash A, Woodward A, Bryant N, McCauley J, Elton D. An efficient genome sequencing method for equine influenza [H3N8] virus reveals a new polymorphism in the PA-X protein.. Virol J 2014 Sep 2;11:159.
                                    doi: 10.1186/1743-422X-11-159pubmed: 25183201google scholar: lookup
                                  32. Collins PJ, Vachieri SG, Haire LF, Ogrodowicz RW, Martin SR, Walker PA, Xiong X, Gamblin SJ, Skehel JJ. Recent evolution of equine influenza and the origin of canine influenza.. Proc Natl Acad Sci U S A 2014 Jul 29;111(30):11175-80.
                                    doi: 10.1073/pnas.1406606111pubmed: 25024224google scholar: lookup
                                  33. Pouwels HG, Van de Zande SM, Horspool LJ, Hoeijmakers MJ. Efficacy of a non-updated, Matrix-C-based equine influenza subunit-tetanus vaccine following Florida sublineage clade 2 challenge.. Vet Rec 2014 Jun 21;174(25):633.
                                    doi: 10.1136/vr.101993pubmed: 24795071google scholar: lookup
                                  34. Bera BC, Virmani N, Shanmugasundaram K, Vaid RK, Singh BK, Gulati BR, Anand T, Barua S, Malik P, Singh RK. Genetic Analysis of the Neuraminidase (NA) Gene of Equine Influenza Virus (H3N8) from Epizootic of 2008-2009 in India.. Indian J Virol 2013 Sep;24(2):256-64.
                                    doi: 10.1007/s13337-013-0137-0pubmed: 24426284google scholar: lookup
                                  35. Gildea S, Fitzpatrick DA, Cullinane A. Epidemiological and virological investigations of equine influenza outbreaks in Ireland (2010-2012).. Influenza Other Respir Viruses 2013 Dec;7 Suppl 4(Suppl 4):61-72.
                                    doi: 10.1111/irv.12192pubmed: 24224821google scholar: lookup
                                  36. Zhu C, Li Q, Guo W, Lu G, Yin X, Qi T, Xiang W, Ran D, Qu J. Complete Genomic Sequences of an H3N8 Equine Influenza Virus Strain Isolated in China.. Genome Announc 2013 Aug 22;1(4).
                                    doi: 10.1128/genomeA.00654-13pubmed: 23969056google scholar: lookup
                                  37. Lu G, Guo W, Qi T, Ma J, Zhao S, Tian Z, Pan J, Zhu C, Wang X, Xiang W. Genetic analysis of the PB1-F2 gene of equine influenza virus.. Virus Genes 2013 Oct;47(2):250-8.
                                    doi: 10.1007/s11262-013-0935-xpubmed: 23780220google scholar: lookup
                                  38. Daly JM, Newton JR, Wood JL, Park AW. What can mathematical models bring to the control of equine influenza?. Equine Vet J 2013 Nov;45(6):784-8.
                                    doi: 10.1111/evj.12104pubmed: 23679041google scholar: lookup
                                  39. Hughes J, Allen RC, Baguelin M, Hampson K, Baillie GJ, Elton D, Newton JR, Kellam P, Wood JL, Holmes EC, Murcia PR. Transmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.. PLoS Pathog 2012 Dec;8(12):e1003081.
                                    doi: 10.1371/journal.ppat.1003081pubmed: 23308065google scholar: lookup
                                  40. Yondon M, Heil GL, Burks JP, Zayat B, Waltzek TB, Jamiyan BO, McKenzie PP, Krueger WS, Friary JA, Gray GC. Isolation and characterization of H3N8 equine influenza A virus associated with the 2011 epizootic in Mongolia.. Influenza Other Respir Viruses 2013 Sep;7(5):659-65.
                                    doi: 10.1111/irv.12069pubmed: 23289427google scholar: lookup
                                  41. Ault A, Zajac AM, Kong WP, Gorres JP, Royals M, Wei CJ, Bao S, Yang ZY, Reedy SE, Sturgill TL, Page AE, Donofrio-Newman J, Adams AA, Balasuriya UB, Horohov DW, Chambers TM, Nabel GJ, Rao SS. Immunogenicity and clinical protection against equine influenza by DNA vaccination of ponies.. Vaccine 2012 Jun 6;30(26):3965-74.
                                    doi: 10.1016/j.vaccine.2012.03.026pubmed: 22449425google scholar: lookup
                                  42. Bountouri M, Fragkiadaki E, Ntafis V, Kanellos T, Xylouri E. Phylogenetic and molecular characterization of equine H3N8 influenza viruses from Greece (2003 and 2007): evidence for reassortment between evolutionary lineages.. Virol J 2011 Jul 14;8:350.
                                    doi: 10.1186/1743-422X-8-350pubmed: 21756308google scholar: lookup
                                  43. Murcia PR, Wood JL, Holmes EC. Genome-scale evolution and phylodynamics of equine H3N8 influenza A virus.. J Virol 2011 Jun;85(11):5312-22.
                                    doi: 10.1128/JVI.02619-10pubmed: 21430049google scholar: lookup
                                  44. Yamanaka T, Tsujimura K, Kondo T, Matsumura T, Ishida H, Kiso M, Hidari KI, Suzuki T. Infectivity and pathogenicity of canine H3N8 influenza A virus in horses.. Influenza Other Respir Viruses 2010 Nov;4(6):345-51.
                                    doi: 10.1111/j.1750-2659.2010.00157.xpubmed: 20958928google scholar: lookup
                                  45. Cullinane A, Elton D, Mumford J. Equine influenza - surveillance and control.. Influenza Other Respir Viruses 2010 Nov;4(6):339-44.
                                    doi: 10.1111/j.1750-2659.2010.00176.xpubmed: 20958927google scholar: lookup
                                  46. Hayward JJ, Dubovi EJ, Scarlett JM, Janeczko S, Holmes EC, Parrish CR. Microevolution of canine influenza virus in shelters and its molecular epidemiology in the United States.. J Virol 2010 Dec;84(24):12636-45.
                                    doi: 10.1128/JVI.01350-10pubmed: 20943966google scholar: lookup
                                  47. Keiner B, Maenz B, Wagner R, Cattoli G, Capua I, Klenk HD. Intracellular distribution of NS1 correlates with the infectivity and interferon antagonism of an avian influenza virus (H7N1).. J Virol 2010 Nov;84(22):11858-65.
                                    doi: 10.1128/JVI.01011-10pubmed: 20844052google scholar: lookup
                                  48. Murcia PR, Baillie GJ, Daly J, Elton D, Jervis C, Mumford JA, Newton R, Parrish CR, Hoelzer K, Dougan G, Parkhill J, Lennard N, Ormond D, Moule S, Whitwham A, McCauley JW, McKinley TJ, Holmes EC, Grenfell BT, Wood JL. Intra- and interhost evolutionary dynamics of equine influenza virus.. J Virol 2010 Jul;84(14):6943-54.
                                    doi: 10.1128/JVI.00112-10pubmed: 20444896google scholar: lookup
                                  49. Koelle K, Khatri P, Kamradt M, Kepler TB. A two-tiered model for simulating the ecological and evolutionary dynamics of rapidly evolving viruses, with an application to influenza.. J R Soc Interface 2010 Sep 6;7(50):1257-74.
                                    doi: 10.1098/rsif.2010.0007pubmed: 20335193google scholar: lookup
                                  50. Bryant NA, Paillot R, Rash AS, Medcalf E, Montesso F, Ross J, Watson J, Jeggo M, Lewis NS, Newton JR, Elton DM. Comparison of two modern vaccines and previous influenza infection against challenge with an equine influenza virus from the Australian 2007 outbreak.. Vet Res 2010 Mar-Apr;41(2):19.
                                    doi: 10.1051/vetres/2009067pubmed: 19863903google scholar: lookup
                                  51. Lu Z, Chambers TM, Boliar S, Branscum AJ, Sturgill TL, Timoney PJ, Reedy SE, Tudor LR, Dubovi EJ, Vickers ML, Sells S, Balasuriya UB. Development and evaluation of one-step TaqMan real-time reverse transcription-PCR assays targeting nucleoprotein, matrix, and hemagglutinin genes of equine influenza virus.. J Clin Microbiol 2009 Dec;47(12):3907-13.
                                    doi: 10.1128/JCM.00598-09pubmed: 19846644google scholar: lookup
                                  52. Chen JM, Sun YX, Chen JW, Liu S, Yu JM, Shen CJ, Sun XD, Peng D. Panorama phylogenetic diversity and distribution of type A influenza viruses based on their six internal gene sequences.. Virol J 2009 Sep 8;6:137.
                                    doi: 10.1186/1743-422X-6-137pubmed: 19737421google scholar: lookup
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