Antimicrobial resistance and genetic characteristics of Salmonella Typhimurium isolated from horses in Hokkaido, Japan.
Abstract: In this study, we examined the antimicrobial susceptibility of 16 Salmonella Typhimurium isolates obtained from horses, and applied several genetic methods, namely polymerase chain reaction (PCR) for detecting class 1 integrons, multiplex PCR for detecting multidrug resistant S. Typhimurium definitive phage type 104 (MR-DT104), and fluorescent amplified-fragment length polymorphism (FAFLP). Seven isolates with an ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline (ACSSuT) type resistance pattern, harbored two class 1 integrons with sizes of 1.2 and 1.0 kb, and were identified as DT104 by bacteriophage typing. These isolates also showed a typical MR-DT104 amplification pattern, which was positive for flo(st), spvC, invA and int, in multiplex PCR. In the FAFLP analysis, the equine DT104 isolates and the previously reported ACSSuT-type resistant bovine isolates, which were also isolated in Hokkaido were included in the same genetic cluster. Our results retrospectively indicate that MR-DT104 infection has existed in horses in Japan at least since 1996, and it was suggested that there is a highly epidemiological relationship between the equine MR-DT104 isolates and certain multidrug resistant bovine isolates in the same area.
Publication Date: 2009-09-02 PubMed ID: 19721370DOI: 10.1292/jvms.71.1115Google Scholar: Lookup
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- Journal Article
Summary
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The researchers investigated the antimicrobial resistance and genetic properties of a bacteria strain named Salmonella Typhimurium which was obtained from horses in Hokkaido, Japan. Their findings provide evidence of a particular multi-drug resistant phage type of the bacteria found in horses dated back to 1996, pointing towards a larger epidemiological relationship between this type found in horses and similar multi-drug resistant strains in bovines within the same area.
Exploration of Antimicrobial Resistance
- The team analyzed 16 isolates of Salmonella Typhimurium from horses for antimicrobial susceptibility. This aimed to find out whether common antibiotic treatments would still be effective against this strain of bacteria.
Genetic Methods Applied
- The researchers used several genetic analysis techniques including polymerase chain reaction (PCR) for detecting so-called ‘class 1 integrons’, a type of genetic component that can transfer antibiotic resistance among bacteria.
- They utilized multiplex PCR to detect a multidrug-resistant variant of S. Typhimurium known as definitive phage type 104 (MR-DT104) – these types of bacteria are well-known for their resistance to many commonly used antibiotics.
- They also used fluorescent amplified-fragment length polymorphism (FAFLP), a genetic technique that helps to identify the exact ‘genetic fingerprint’ of specific bacterial isolates, allowing for comparison and identification of similar strains.
Findings and Implications
- Through these techniques, seven isolates exhibiting resistance to multiple types of antibiotics (ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline – ACSSuT type) and containing class 1 integrons were identified as type DT104 through bacteriophage typing.
- These isolates shared a common amplification pattern indicating the presence of multiple genes associated with resistances including flo(st), spvC, invA, and int.
- A comparison through FAFLP analysis revealed that the genetic profiles of the horse DT104 isolates were similar to some multi-drug resistant isolates from bovines, also isolated in Hokkaido.
- The findings suggest the presence of this multi-drug resistant DT104 type not just in bovines but also in horses dating back to at least 1996, implying a prevalent epidemiological link between the strains found in horses and bovines in Hokkaido, Japan.
Cite This Article
APA
Niwa H, Anzai T, Izumiya H, Morita-Ishihara T, Watanabe H, Uchida I, Tozaki T, Hobo S.
(2009).
Antimicrobial resistance and genetic characteristics of Salmonella Typhimurium isolated from horses in Hokkaido, Japan.
J Vet Med Sci, 71(8), 1115-1119.
https://doi.org/10.1292/jvms.71.1115 Publication
Researcher Affiliations
- Equine Research Institute, Japan Racing Association, Tochigi, Japan. niwa@epizoo.equinst.go.jp
MeSH Terms
- Amplified Fragment Length Polymorphism Analysis
- Animals
- Anti-Bacterial Agents / pharmacology
- Bacterial Typing Techniques
- Bacteriophages / genetics
- Bacteriophages / isolation & purification
- Cattle
- DNA Primers
- DNA, Bacterial / genetics
- Drug Resistance, Multiple, Bacterial / drug effects
- Drug Resistance, Multiple, Bacterial / genetics
- Horse Diseases / microbiology
- Horses
- Japan
- Microbial Sensitivity Tests
- Polymerase Chain Reaction
- Restriction Mapping
- Salmonella Infections, Animal / drug therapy
- Salmonella Infections, Animal / genetics
- Salmonella typhimurium / drug effects
- Salmonella typhimurium / genetics
- Salmonella typhimurium / isolation & purification
Citations
This article has been cited 4 times.- Mitchell SW, Moran RA, Elbourne LDH, Chapman B, Bull M, Muscatello G, Coleman NV. Impacts of Domestication and Veterinary Treatment on Mobile Genetic Elements and Resistance Genes in Equine Fecal Bacteria. Appl Environ Microbiol 2023 Mar 29;89(3):e0159022.
- Sato W, Sukmawinata E, Uemura R, Kanda T, Kusano K, Kambayashi Y, Sato T, Ishikawa Y, Toya R, Sueyoshi M. Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan. J Equine Sci 2020;31(4):85-91.
- Sukmawinata E, Sato W, Mitoma S, Kanda T, Kusano K, Kambayashi Y, Sato T, Ishikawa Y, Goto Y, Uemura R, Sueyoshi M. Extended-spectrum β-lactamase-producing Escherichia coli isolated from healthy Thoroughbred racehorses in Japan. J Equine Sci 2019 Sep;30(3):47-53.
- Sasaki Y, Suzuki S, Kusaba N, Rahman N, Aikawa C, Okamura M. Antimicrobial resistance of Salmonella isolates from cattle and horses with salmonellosis in Hokkaido, Japan. J Vet Med Sci 2024 Dec 1;86(12):1227-1232.
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