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Pathogens (Basel, Switzerland)2022; 12(1); 64; doi: 10.3390/pathogens12010064

Antimicrobial Resistance and Genetic Diversity of Pseudomonas aeruginosa Strains Isolated from Equine and Other Veterinary Samples.

Abstract: is one of the leading causes of healthcare-associated infections in humans. This bacterium is less represented in veterinary medicine, despite causing difficult-to-treat infections due to its capacity to acquire antimicrobial resistance, produce biofilms, and persist in the environment, along with its limited number of veterinary antibiotic therapies. Here, we explored susceptibility profiles to antibiotics and to didecyldimethylammonium chloride (DDAC), a quaternary ammonium widely used as a disinfectant, in 168 strains isolated from animals, mainly Equidae. A genomic study was performed on 41 of these strains to determine their serotype, sequence type (ST), relatedness, and resistome. Overall, 7.7% of animal strains were resistant to carbapenems, 10.1% presented a multidrug-resistant (MDR) profile, and 11.3% showed decreased susceptibility (DS) to DDAC. Genomic analyses revealed that the study population was diverse, and 4.9% were ST235, which is considered the most relevant human high-risk clone worldwide. This study found populations with carbapenem resistance, multidrug resistance, and DS to DDAC in equine and canine isolates. These strains, which are not susceptible to antibiotics used in veterinary and human medicine, warrant close the setting up of a clone monitoring, based on that already in place in human medicine, in a one-health approach.
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Publication Date: 2022-12-30 PubMed ID: 36678412PubMed Central: PMC9867525DOI: 10.3390/pathogens12010064Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the antimicrobial resistance and genetic diversity of the bacterium Pseudomonas aeruginosa in different types of veterinary samples, with a focus on equine ones. Importantly, the potential threat posed by this bacterium and the need for a surveillance system for clones are highlighted.

Overview of the Research and Methodology

  • This study aimed to understand the antibiotic resistance profiles of Pseudomonas aeruginosa strains, their ability to persist in diverse environments, and their capacity to form biofilms.
  • The research focused on 168 strains of P. aeruginosa mainly derived from animals of the equidae family. It studied their susceptibility profiles to various antibiotics as well as to didecyldimethylammonium chloride (DDAC), a common disinfectant.
  • Furthermore, a detailed genomic analysis was conducted on 41 of these strains to understand their serotype, sequence type, genetic similarity and resistance profiles – the collection of antibiotic resistance genes they carry.

Major Findings of the Research

  • The research provides important insights into the spread of drug resistance among P. aeruginosa strains in veterinary settings.
  • It was found that 7.7% of the animal strains were resistant to carbapenems, a class of antibiotics used to treat severe or high-risk bacterial infections. Additionally, 10.1% of the strains demonstrated a multidrug-resistant (MDR) profile, and 11.3% showed reduced susceptibility to the disinfectant DDAC.
  • Genomic analyses revealed a diverse study population, with 4.9% of the strains being ST235, a clone considered to be a significant high-risk strain in human medical contexts.

Implications of the Research

  • This study highlights the presence of P. aeruginosa strains in animal populations that have developed resistance to antibiotics commonly used in both human and veterinary medicine.
  • It suggests the need for establishing monitoring systems for tracking these potentially dangerous bacterial strains, following similar practices already in place in human medicine.
  • The findings underscore the importance of the one-health approach that recognizes the interconnectedness of human health, animal health, and environmental health.

Cite This Article

APA
Pottier M, Castagnet S, Gravey F, Leduc G, Sévin C, Petry S, Giard JC, Le Hello S, Léon A. (2022). Antimicrobial Resistance and Genetic Diversity of Pseudomonas aeruginosa Strains Isolated from Equine and Other Veterinary Samples. Pathogens, 12(1), 64. https://doi.org/10.3390/pathogens12010064

Publication

Pathogens (Basel, Switzerland)
ISSN: 2076-0817
NlmUniqueID: 101596317
Country: Switzerland
Language: English
Volume: 12
Issue: 1
PII: 64

Researcher Affiliations

Pottier, Marine
  • Research Department, LABÉO, 14053 Caen, France.
  • Inserm UMR 1311, Dynamicure, Normandie University, UNICAEN, UNIROUEN, 14000 Caen, France.
Castagnet, Sophie
  • Research Department, LABÉO, 14053 Caen, France.
  • Inserm UMR 1311, Dynamicure, Normandie University, UNICAEN, UNIROUEN, 14000 Caen, France.
Gravey, François
  • Inserm UMR 1311, Dynamicure, Normandie University, UNICAEN, UNIROUEN, 14000 Caen, France.
  • CHU de Caen, Service de Microbiologie, Avenue de la Côte de Nacre, 14033 Caen, France.
Leduc, Guillaume
  • CHU de Caen, Service de Microbiologie, Avenue de la Côte de Nacre, 14033 Caen, France.
Sévin, Corinne
  • Anses, Normandy Laboratory for Animal Health, Physiopathology and Epidemiology of Equine Diseases Unit, 14430 Goustranville, France.
Petry, Sandrine
  • Anses, Normandy Laboratory for Animal Health, Physiopathology and Epidemiology of Equine Diseases Unit, 14430 Goustranville, France.
Giard, Jean-Christophe
  • Inserm UMR 1311, Dynamicure, Normandie University, UNICAEN, UNIROUEN, 14000 Caen, France.
Le Hello, Simon
  • Inserm UMR 1311, Dynamicure, Normandie University, UNICAEN, UNIROUEN, 14000 Caen, France.
  • CHU de Caen, Service de Microbiologie, Avenue de la Côte de Nacre, 14033 Caen, France.
  • CHU de Caen, Service d'Hygiène Hospitalière, Avenue de la Côte de Nacre, 14033 Caen, France.
Léon, Albertine
  • Research Department, LABÉO, 14053 Caen, France.
  • Inserm UMR 1311, Dynamicure, Normandie University, UNICAEN, UNIROUEN, 14000 Caen, France.

Grant Funding

  • AMI2019RIN50_MPOTTIER / Normandy Regional Council
  • 2020-206-29-062 / Ministu00e8re de l'agriculture et de l'alimentation

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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