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Home / Equine Research Bank / PLoS One / Antimicrobial resistance in coagulase-positive staphylococci...
PloS one2017; 12(4); e0176379; doi: 10.1371/journal.pone.0176379

Antimicrobial resistance in coagulase-positive staphylococci isolated from companion animals in Australia: A one year study.

Abstract: Methicillin-resistant coagulase-positive staphylococci (CoPS) have become increasingly recognised as opportunistic pathogens that limit therapeutic options in companion animals. The frequency of methicillin resistance amongst clinical isolates on an Australia-wide level is unknown. This study determined antimicrobial susceptibility patterns for CoPS isolated from clinical infections in companion animals (dogs, cats and horses) as part of the first nation-wide survey on antimicrobial resistance in animal pathogens in Australia for a one-year period (January 2013 to January 2014). Clinical Staphylococcus spp. isolates (n = 888) obtained from 22 veterinary diagnostic laboratories were identified by MALDI-TOF mass spectrometry and subjected to antimicrobial susceptibility testing for 16 antimicrobials, representing 12 antimicrobial classes. Potential risk factors associated with methicillin resistance in Staphylococcus pseudintermedius isolates from dogs were analysed based on demographic factors and clinical history, including gender, age, previous antimicrobial treatment, chronic and/or recurrent diseases and site of infections. The most commonly identified CoPS were S. pseudintermedius (70.8%; dogs n = 616, cats n = 13) and S. aureus (13.2%, horses n = 53, dogs n = 47 and cats n = 17). Overall, the frequency of methicillin resistance among S. pseudintermedius (MRSP) and S. aureus (MRSA) was 11.8% and 12.8%, respectively. MRSP isolates were strongly associated with resistance to fluoroquinolones (OR 287; 95%CI 91.2-1144.8) and clindamycin (OR 105.2, 95%CI 48.5-231.9). MRSA isolates from dogs and cats were also more likely to be resistant to fluoroquinolones (OR 5.4, 95%CI 0.6-252.1), whereas MRSA from horses were more likely to be resistant to rifampicin. In multivariate analysis, MRSP-positive status was significantly associated with particular infection sites, including surgical (OR 8.8; 95%CI 3.74-20.7), and skin and soft tissue (OR 3.9; 95%CI 1.97-7.51). S. pseudintermedius isolated from dogs with surgical site infections were three times more likely to be methicillin-resistant if cases had received prior antimicrobial treatment. Whilst the survey results indicate the proportion of CoPS obtained from Australian companion animals that are methicillin-resistant is currently moderate, the identified risk factors suggest that it could rapidly increase without adequate biosecurity and infection control procedures in veterinary practice.
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Publication Date: 2017-04-21 PubMed ID: 28430811PubMed Central: PMC5400250DOI: 10.1371/journal.pone.0176379Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research is a one-year study evaluating the frequency and risk factors of methicillin-resistant bacteria in common pets in Australia, which might reduce available treatment options.

Research Objectives

  • The core aim of this research was to uncover the frequency of methicillin resistance in coagulase-positive staphylococci (CoPS), a type of bacteria isolated from clinical infections in companion animals, namely dogs, cats, and horses.
  • This stemmed from a larger concern that these animals could become reservoirs for methicillin-resistant strains, limiting potential treatment routes.
  • The research also sought to ascertain risk factors associated with methicillin resistance in Staphylococcus pseudintermedius, a common dog skin pathogen.

Methodology

  • Staphylococcus spp. isolates (888 of them) from 22 veterinary diagnostic laboratories were used for testing and identified using a technique known as MALDI-TOF mass spectrometry.
  • The susceptibility patterns of the isolates for 16 antimicrobials from 12 antimicrobial classes were determined.
  • Demographic factors, clinical history, and site of infections were studied. These data elements included gender, age, previous antimicrobial treatment, chronic and/or recurrent diseases.

Findings and Implications

  • The types of CoPS most commonly found were S. pseudintermedius (mainly in dogs and cats), and S. aureus (in dogs, cats, and horses).
  • Resistance to methicillin was found to be present at a rate of 11.8% in S. pseudintermedius, and 12.8% in S. aureus.
  • Methicillin-resistant strains showed a strong association with resistance to fluoroquinolones and clindamycin in the case of S. pseudintermedius, and to fluoroquinolones and rifampicin in the case of S. aureus from dogs, cats, and horses.
  • In multivariate analysis, there was a significant association between methicillin-resistant S. pseudintermedius-positive status and specific infection sites, including surgical and skin and soft tissue.
  • Furthermore, S. pseudintermedius from dogs with surgical site infections was found to be thrice as likely to be methicillin-resistant if the animal had received prior antimicrobial treatment.
  • Despite the currently moderate percentage of methicillin-resistant CoPS in Australian pets, the identified risk factors suggest the possibility of a swift increase if veterinary practices are not modified to incorporate better biosecurity and infection control procedures.

Cite This Article

APA
Saputra S, Jordan D, Worthing KA, Norris JM, Wong HS, Abraham R, Trott DJ, Abraham S. (2017). Antimicrobial resistance in coagulase-positive staphylococci isolated from companion animals in Australia: A one year study. PLoS One, 12(4), e0176379. https://doi.org/10.1371/journal.pone.0176379

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 12
Issue: 4
Pages: e0176379

Researcher Affiliations

Saputra, Sugiyono
  • Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA, Australia.
  • Research Center for Biology, Indonesian Institute of Sciences, Cibinong, West Java, Indonesia.
Jordan, David
  • New South Wales Department of Primary Industries, Wollongbar, NSW, Australia.
Worthing, Kate A
  • Faculty of Veterinary Science, The University of Sydney, Camperdown, NSW, Australia.
Norris, Jacqueline M
  • Faculty of Veterinary Science, The University of Sydney, Camperdown, NSW, Australia.
Wong, Hui S
  • Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA, Australia.
Abraham, Rebecca
  • Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA, Australia.
  • School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia.
Trott, Darren J
  • Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA, Australia.
Abraham, Sam
  • Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA, Australia.
  • School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia.

MeSH Terms

  • Animals
  • Australia
  • Cats / microbiology
  • Coagulase / metabolism
  • Dogs / microbiology
  • Microbial Sensitivity Tests
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / enzymology

Conflict of Interest Statement

Competing Interests: DT, SA and JN have received contract research grants and consulting funds from Bayer, Zoetis, Virbac, and Elanco. This does not alter our adherence to PLOS ONE policies on sharing data and materials.

References

This article includes 43 references
  1. Savini V, Passeri C, Mancini G, Iuliani O, Marrollo R, Argentieri AV. Coagulase-positive staphylococci: my pet's two faces.. Res Microbiol 2013;164(5):371–4.
    doi: 10.1016/j.resmic.2013.02.004pubmed: 23481095google scholar: lookup
  2. Ballhausen B, Kriegeskorte A, Schleimer N, Peters G, Becker K. The mecA homolog mecC confers resistance against beta-lactams in Staphylococcus aureus irrespective of the genetic strain background.. Antimicrob Agents Chemother 2014;58(7):3791–8.
    doi: 10.1128/AAC.02731-13pmc: PMC4068569pubmed: 24752255google scholar: lookup
  3. Johnson AP. Methicillin-resistant Staphylococcus aureus: the European landscape.. J Antimicrob Chemother 2011;66 Suppl 4:iv43–iv8.
    pubmed: 21521706
  4. Ruscher C, Lubke-Becker A, Semmler T, Wleklinski CG, Paasch A, Soba A. Widespread rapid emergence of a distinct methicillin- and multidrug-resistant Staphylococcus pseudintermedius (MRSP) genetic lineage in Europe.. Veterinary Microbio 2010;144(3–4):340–6.
    pubmed: 20181441
  5. Smith TC. Livestock-associated Staphylococcus aureus: the United States experience.. PLoS Pathog 2015;11(2):e1004564.
    doi: 10.1371/journal.ppat.1004564pmc: PMC4412291pubmed: 25654425google scholar: lookup
  6. Gingrich EN, Kurt T, Hyatt DR, Lappin MR, Ruch-Gallie R. Prevalence of methicillin-resistant staphylococci in Northern Colorado shelter animals.. J Vet Diagn Invest 2011;23(5):947–50.
    doi: 10.1177/1040638711407301pubmed: 21908352google scholar: lookup
  7. Jordan D, Simon J, Fury S, Moss S, Giffard P, Maiwald M. Carriage of methicillin-resistant Staphylococcus aureus by veterinarians in Australia.. Aust Vet Jo 2011;89(5):152–9.
    pubmed: 21495985
  8. Walther B, Monecke S, Ruscher C, Friedrich AW, Ehricht R, Slickers P. Comparative molecular analysis substantiates zoonotic potential of equine methicillin-resistant Staphylococcus aureus.. J Clin Microbiol 2009;47(3):704–10.
    doi: 10.1128/JCM.01626-08pmc: PMC2650932pubmed: 19109463google scholar: lookup
  9. Harrison EM, Weinert LA, Holden MT, Welch JJ, Wilson K, Morgan FJ. A shared population of epidemic methicillin-resistant Staphylococcus aureus 15 circulates in humans and companion animals.. mBio 2014;5(3):e00985–13.
    pmc: PMC4030480pubmed: 24825010
  10. van Duijkeren E, Kamphuis M, van der Mije IC, Laarhoven LM, Duim B, Wagenaar JA. Transmission of methicillin-resistant Staphylococcus pseudintermedius between infected dogs and cats and contact pets, humans and the environment in households and veterinary clinics.. Vet Microbiol 2011;150(3–4):338–43.
    doi: 10.1016/j.vetmic.2011.02.012pubmed: 21420256google scholar: lookup
  11. Siak M, Burrows AK, Coombs GW, Khazandi M, Abraham S, Norris JM. Characterization of meticillin-resistant and meticillin-susceptible isolates of Staphylococcus pseudintermedius from cases of canine pyoderma in Australia.. J Med Microbiol 2014;63(Pt 9):1228–33.
    doi: 10.1099/jmm.0.076117-0pubmed: 24994658google scholar: lookup
  12. Guardabassi L, Schwarz S, Lloyd DH. Pet animals as reservoirs of antimicrobial-resistant bacteria.. J Antimicrob Chemother 2004;54(2):321–32.
    doi: 10.1093/jac/dkh332pubmed: 15254022google scholar: lookup
  13. Youn J-H, Yoon JW, Koo Hye Cheong, Lim S-K, Park YH. Prevalence and antimicrogram of Staphylococcus intermedius group isolates from veterinary staff, companion animals, and the environment in veterinary hospitals in Korea.. J Vet Diagn Invest 2011;23:268–74.
    doi: 10.1177/104063871102300211pubmed: 21398446google scholar: lookup
  14. Feng Y, Tian W, Lin D, Luo Q, Zhou Y, Yang T. Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius in pets from South China.. Vet Microbiol 2012;160(3–4):517–24.
    doi: 10.1016/j.vetmic.2012.06.015pubmed: 22770517google scholar: lookup
  15. Youn JH, Park YH, Hang'ombe B, Sugimoto C. Prevalence and characterization of Staphylococcus aureus and Staphylococcus pseudintermedius isolated from companion animals and environment in the veterinary teaching hospital in Zambia, Africa.. Comp Immunol Microbiol Infect Dis 2014;37(2):123–30.
    doi: 10.1016/j.cimid.2014.01.003pubmed: 24480623google scholar: lookup
  16. Perreten V, Kadlec K, Schwarz S, Gronlund Andersson U, Finn M, Greko C. Clonal spread of methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an international multicentre study.. J Antimicrob Chemother 2010;65(6):1145–54.
    doi: 10.1093/jac/dkq078pubmed: 20348087google scholar: lookup
  17. . Use of antimicrobials and occurrence of antimicrobial resistance in Sweden 2014.. Upsala: 2015.
  18. . Usage of antimicrobial agents and occurrence of antimicrobial resistance in Norway.. Oslo, Norway: 2013.
  19. Groves MD, Crouch B, Coombs GW, Jordan D, Pang S, Barton MD. Molecular Epidemiology of methicillin-resistant Staphylococcus aureus isolated from Australian veterinarians.. PloSOne 2016;11(1):e0146034.
    pmc: PMC4703204pubmed: 26735694
  20. Malik S, Coombs GW, O'Brien FG, Peng H, Barton MD. Molecular typing of methicillin-resistant staphylococci isolated from cats and dogs.. J Antimicrob Chemother 2006;58(2):428–31.
    doi: 10.1093/jac/dkl253pubmed: 16782740google scholar: lookup
  21. Worthing KA, Coombs GW, Pang S, Abraham S, Saputra S, Trott DJ. Isolation of mecC MRSA in Australia.. J Antimicrob Chemother 2016:dkw138.
    pubmed: 27118772
  22. Trott D, Jordan D, Barton M, Abraham S, Groves M. Vets versus pets: methicillin-resistant Staphylococcus aureus in Australian animals and their doctors.. Microbiol Aust 2013;10:25–7.
  23. Abraham S, Jordan D, Wong HS, Johnson JR, Toleman MA, Wakeham DL. First detection of extended-spectrum cephalosporin- and fluoroquinolone-resistant Escherichia coli in Australian food-producing animals.. Journal of Global Antimicrobial Resistance 2015;3(4):273–7.
    doi: 10.1016/j.jgar.2015.08.002pubmed: 27842872google scholar: lookup
  24. . Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; Approved Standard-Fourth Edition.. CLSI document VET01-A4. Wayne, PA, USA: CLSI; 2013.
  25. . Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals- Third Edition.. CLSI document VET01S. Wayne, PA, USA: CLSI; 2015.
  26. Turnidge J, Kahlmeter G, Kronvall G. Statistical characterisation of bacterial wild-type MIC value distributions and the determination of epidemiological cut-off values.. Clin Microbiol Infect 2006;12(5):418–25.
    doi: 10.1111/j.1469-0691.2006.01377.xpubmed: 16643517google scholar: lookup
  27. . ECOFFinder [Internet].. 2016 [cited 10 June 2016]. Available from: http://clsi.org/standards/micro/ecoffinder/..
  28. . Antimicrobial wild type distributions of microorganisms 2016 [cited 10 June 2016].. Available from: http://mic.eucast.org/Eucast2/SearchController/search.jsp?action=init..
  29. Magiorakos A, Srinivasan A, Carey R, Carmeli Y, Falagas M, Giske C. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance.. Clin Microbiol Infect 2012;18:268–81.
    doi: 10.1111/j.1469-0691.2011.03570.xpubmed: 21793988google scholar: lookup
  30. EFSA ECDC. EU Summary Report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2013.. EFSA Journal 2015;13(2):1–178.
  31. Merlino J, Watson J, Rose B, Beard-Pegler M, Gottlieb T, Bradbury R. Detection and expression of methicillin/oxacillin resistance in multidrug-resistant and non-multidrug-resistant Staphylococcus aureus in Central Sydney, Australia.. J Antimicrob Chemother 2002;49(5):793–801.
    pubmed: 12003973
  32. Gold RM, Cohen ND, Lawhon SD. Amikacin resistance in Staphylococcus pseudintermedius isolated from dogs.. J Clin Microbiol 2014;52(10):3641–6.
    doi: 10.1128/JCM.01253-14pmc: PMC4187778pubmed: 25078911google scholar: lookup
  33. Shaban R, Simon G, Trott D, Turnidge J, Jordan D. Surveillance and reporting of antimicrobial resistance and antibiotic usage in animals and agriculture in Australia.. Canberra, Australia: Department of Agriculture, The Australian Government, 2014.
  34. Malik S, Peng H, Barton MD. Partial nucleotide sequencing of the mecA genes of Staphylococcus aureus isolates from cats and dogs.. J Clin Microbiol 2006;44(2):413–6.
    doi: 10.1128/JCM.44.2.413-416.2006pmc: PMC1392638pubmed: 16455893google scholar: lookup
  35. Axon JE, Carrick JB, Barton MD, Collins NM, Russell CM, Kiehne J. Methicillin-resistant Staphylococcus aureus in a population of horses in Australia.. Aust Vet J 2011;89(6):221–5.
    doi: 10.1111/j.1751-0813.2011.00711.xpubmed: 21595643google scholar: lookup
  36. Crowley J, Po E, Celi P, Muscatello G. Systemic and respiratory oxidative stress in the pathogenesis and diagnosis of Rhodococcus equi pneumonia.. Equine Vet J Suppl 2013;(45):20–5.
    doi: 10.1111/evj.12166pubmed: 24304399google scholar: lookup
  37. Solyman SM, Black CC, Duim B, Perreten V, van Duijkeren E, Wagenaar JA. Multilocus sequence typing for characterization of Staphylococcus pseudintermedius.. J Clin Microbiol 2013;51(1):306–10.
    doi: 10.1128/JCM.02421-12pmc: PMC3536184pubmed: 23115265google scholar: lookup
  38. Paul NC, Moodley A, Ghibaudo G, Guardabassi L. Carriage of methicillin-resistant Staphylococcus pseudintermedius in small animal veterinarians: indirect evidence of zoonotic transmission.. Zoonoses Public Health 2011;58(8):533–9.
    doi: 10.1111/j.1863-2378.2011.01398.xpubmed: 21824350google scholar: lookup
  39. Enrright MC, Day NPJ, Davies CE, Peacock SJ, Sprati BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus.. J Clin Microbiol 2000;38(3):1008–15.
    pmc: PMC86325pubmed: 10698988
  40. Soares Magalhaes RJ, Loeffler A, Lindsay J, Rich M, Roberts L, Smith H. Risk factors for methicillin-resistant Staphylococcus aureus (MRSA) infection in dogs and cats: a case-control study.. Vet Res 2010;41(5):55.
    doi: 10.1051/vetres/2010028pmc: PMC2879574pubmed: 20423695google scholar: lookup
  41. Lehner G, Linek M, Bond R, Lloyd DH, Prenger-Berninghoff E, Thom N. Case-control risk factor study of methicillin-resistant Staphylococcus pseudintermedius (MRSP) infection in dogs and cats in Germany.. Vet Microbiol 2014;168(1):154–60.
    doi: 10.1016/j.vetmic.2013.10.023pubmed: 24290489google scholar: lookup
  42. . Developing a national antimicrobial resistance strategy for Australia [Internet].. Australian Veterinary Association; 2014 [Cited 10 June 2016]; [1–5]. Available from: http://www.ava.com.au/sites/default/files/AVA_website/pdfs/AVA%20AMR%20strategy%20discussion%20paper%20submission%20FINAL.pdf.
  43. Gandolfi-Decristophoris P, Regula G, Petrini O, Zinsstag J, Schelling E. Prevalence and risk factors for carriage of multi-drug resistant Staphylococci in healthy cats and dogs.. J Vet Sci 2013;14(4):449.
    doi: 10.4142/jvs.2013.14.4.449pmc: PMC3885739pubmed: 23820161google scholar: lookup
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