Area under the curve calculations as a tool to compare the efficacy of equine influenza vaccines–a retrospective analysis of three independent field trials.
Abstract: Using the area under the curve (AUC) concept as is commonly used in pharmaceutical bioequivalence studies, the bioequivalence of three equine influenza vaccines was demonstrated. A retrospective analysis was performed using this technique on data generated in three trials in which each of the three vaccines had been used. In total, data from 63 pony and horse foals were used. The AUC of the single radial hemolysis (SRH) titres against Influenza A/equi-1/Prague/56 (Pr/56), A/equi-2/Newmarket-1/93, and A/equi-2/Suffolk/89 (Suf/89) were calculated for each horse. It was concluded that calculation of the AUC from four time-points permitted a suitable estimate for vaccine potency. Using pooled data, it appeared that the AUC permitted better evaluation of vaccine potency than simply considering the highest post vaccinal titre (Titremax). In two studies, a minimal value for the AUC was associated with protection against Influenza (H3N8) challenge 50-153 days later.
Publication Date: 2002-08-23 PubMed ID: 12191504DOI: 10.1016/s0022-1759(01)00571-3Google Scholar: Lookup
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- Clinical Trial
- Comparative Study
- Journal Article
Summary
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The research article is about a retrospective analysis of three independent field trials, using the area under the curve (AUC) calculation tool to compare the effectiveness of three different equine influenza vaccines.
Objective of the Research
- The main objective of this research was to utilize the concept of AUC, commonly used in pharmaceutical bioequivalence studies, to assess the bioequivalence of three different equine influenza vaccines. The idea was to see if utilizing AUC calculations would provide a more detailed understanding of the vaccine’s potency as compared to merely considering the highest post-vaccination titre (Titremax).
Methodology Involved
- Researchers undertook a retrospective analysis of data from three different trials where each of these vaccines had been utilized. The trials involved a total of 63 pony and horse foals.
- The researchers calculated the AUC of the single radial hemolysis (SRH) titres against Influenza A/equi-1/Prague/56 (Pr/56), A/equi-2/Newmarket-1/93, and A/equi-2/Suffolk/89 (Suf/89) for each horse.
- Four time-points were selected for calculating the AUC for each horse to provide a good estimate of the potency of the vaccines.
Results and Findings
- The results from the analysis showed that calculating the AUC offered a more accurate estimate of the potency of the vaccine, as compared to simply considering the highest post-vaccination titre (Titremax). This conclusion was based on data pooled from all studies.
- In two of the studies, results demonstrated that a low value for the AUC was linked to successful protection against Influenza (H3N8) challenge 50-153 days after the initial vaccination. This suggests that the AUC method is effective in predicting the long-term efficacy of equine influenza vaccines.
Cite This Article
APA
Heldens JG, Weststrate MW, van den Hoven R.
(2002).
Area under the curve calculations as a tool to compare the efficacy of equine influenza vaccines–a retrospective analysis of three independent field trials.
J Immunol Methods, 264(1-2), 11-17.
https://doi.org/10.1016/s0022-1759(01)00571-3 Publication
Researcher Affiliations
- Department of Biological Research and Development, Fort Dodge Animal Health Holland, Post Box 36, 1381 AA Weesp, The Netherlands.
MeSH Terms
- Animals
- Antibodies, Viral / biosynthesis
- Antibodies, Viral / blood
- Area Under Curve
- Blood Specimen Collection / statistics & numerical data
- Blood Specimen Collection / veterinary
- Horse Diseases / immunology
- Horse Diseases / prevention & control
- Horses
- Immunization, Secondary / statistics & numerical data
- Immunization, Secondary / veterinary
- Immunodiffusion / statistics & numerical data
- Immunodiffusion / veterinary
- Influenza A virus / immunology
- Influenza Vaccines / administration & dosage
- Influenza Vaccines / therapeutic use
- Injections, Intramuscular
- Orthomyxoviridae Infections / immunology
- Orthomyxoviridae Infections / prevention & control
- Orthomyxoviridae Infections / veterinary
- Retrospective Studies
- Therapeutic Equivalency
- Vaccination / statistics & numerical data
- Vaccination / veterinary
Citations
This article has been cited 6 times.- Allkofer A, Garvey M, Ryan E, Lyons R, Ryan M, Lukaseviciute G, Walsh C, Venner M, Cullinane A. Primary vaccination in foals: a comparison of the serological response to equine influenza and equine herpesvirus vaccines administered concurrently or 2 weeks apart. Arch Virol 2021 Feb;166(2):571-579.
- Entenfellner J, Gahan J, Garvey M, Walsh C, Venner M, Cullinane A. Response of Sport Horses to Different Formulations of Equine Influenza Vaccine. Vaccines (Basel) 2020 Jul 10;8(3).
- Melville K, Rodriguez T, Dobrovolny HM. Investigating Different Mechanisms of Action in Combination Therapy for Influenza. Front Pharmacol 2018;9:1207.
- Coughlan L, Sridhar S, Payne R, Edmans M, Milicic A, Venkatraman N, Lugonja B, Clifton L, Qi C, Folegatti PM, Lawrie AM, Roberts R, de Graaf H, Sukhtankar P, Faust SN, Lewis DJM, Lambe T, Hill A, Gilbert SC. Heterologous Two-Dose Vaccination with Simian Adenovirus and Poxvirus Vectors Elicits Long-Lasting Cellular Immunity to Influenza Virus A in Healthy Adults. EBioMedicine 2018 Mar;29:146-154.
- Gildea S, Sanchez Higgins MJ, Johnson G, Walsh C, Cullinane A. Concurrent vaccination against equine influenza and equine herpesvirus - a practical approach. Influenza Other Respir Viruses 2016 Sep;10(5):433-7.
- Kelley ME, Franco AR, Mayberg HS, Holtzheimer PE. The Illness Density Index (IDI): A longitudinal measure of treatment efficacy. Clin Trials 2012 Oct;9(5):596-604.
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