Assessing the variability of Brazilian Vaccinia virus isolates from a horse exanthematic lesion: coinfection with distinct viruses.
Abstract: During the last bovine vaccinia (BV) outbreaks, several Vaccinia virus (VACV) strains were isolated and characterised, revealing significant polymorphisms between strains, even within conserved genes. Although the epidemiology of VACV has been studied in BV outbreaks, there is little data about the circulation of the Brazilian VACV isolates. This study describes the genetic and biological characterisation of two VACV isolates, Pelotas 1 virus (P1V) and Pelotas 2 virus (P2V), which were obtained concomitantly from a horse affected by severe cutaneous disease. Despite being isolated from the same exanthematic clinical sample, P1V and P2V showed differences in their plaque phenotype and in one-step growth curves. Moreover, P1V and P2V presented distinct virulence profiles in a BALB/c mouse model, as observed with other Brazilian VACV isolates. Sequencing and phylogenetic analysis of four different genes demonstrated that the isolates are segregated in different VACV clusters. Our results raise interesting questions about the diversity of VACV isolates in Brazil.
Publication Date: 2010-11-16 PubMed ID: 21080203DOI: 10.1007/s00705-010-0857-zGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article explores the variability and characteristics of two Vaccinia virus strains, Pelotas 1 virus and Pelotas 2 virus, extracted from a horse affected by severe skin disease. The study finds noticeable differences between the two strains’ growth patterns, virulence profiles, and gene sequencing, suggesting a greater diversity among Vaccinia virus strains in Brazil than previously known.
Exploring the Variability of Vaccinia Virus Strains
- The paper notes that researchers have isolated and examined numerous Vaccinia virus (VACV) strains during recent bovine vaccinia outbreaks. These studies revealed substantial polymorphisms—or genetic variations—between the strains, even concerning genes typically regarded as conserved or unchanging.
- However, the study points to a lack of data about the circulation of Brazilian VACV isolates, which this research aims to address by studying two VACV strains. These strains, dubbed Pelotas 1 virus (P1V) and Pelotas 2 virus (P2V), were isolated concurrently from a horse suffering from a severe cutaneous disease.
Differences between P1V and P2V Strains
- Despite both strains being isolated from the same clinical sample, P1V and P2V exhibited differences in their plaque phenotype, which is a term used to describe the visible effects of a virus on infected cells observed in laboratory experiments.
- The researchers also observed differences in one-step growth curves, suggesting the two virus strains multiply at different rates.
- The virulence profiles—or the extent of the disease-causing capability—of P1V and P2V were distinct, mirroring the diversity found in other Brazilian VACV isolates.
Genetic Divergences
- Sequencing and subsequent phylogenetic analysis of four different genes demonstrated that these isolates fall into different VACV clusters. This segregation suggests a more extensive genetic divergence between the strains.
Significance of the Study
- This research reveals that there is a greater diversity among VACV isolates in Brazil than previously understood, posing interesting questions that need further investigation. It could potentially lead to more effective outbreak control strategies and nuanced vaccine development, given the considerable genetic variation and differing virulence found even among viruses isolated from the same sample.
Cite This Article
APA
Campos RK, Brum MC, Nogueira CE, Drumond BP, Alves PA, Siqueira-Lima L, Assis FL, Trindade GS, Bonjardim CA, Ferreira PC, Weiblen R, Flores EF, Kroon EG, Abrahu00e3o JS.
(2010).
Assessing the variability of Brazilian Vaccinia virus isolates from a horse exanthematic lesion: coinfection with distinct viruses.
Arch Virol, 156(2), 275-283.
https://doi.org/10.1007/s00705-010-0857-z Publication
Researcher Affiliations
- Departamento de Microbiologia, Instituto de Ciu00eancias Biolu00f3gicas, Laboratu00f3rio de Vu00edrus, Universidade Federal de Minas Gerais, Av. Antu00f4nio Carlos 6627, Pampulha, Belo Horizonte, Minas Gerais, Brazil.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Brazil
- Cattle
- DNA, Viral / genetics
- Exanthema / veterinary
- Exanthema / virology
- Genes, Viral
- Hemagglutinins, Viral / genetics
- Horse Diseases / virology
- Horses
- Male
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Phylogeny
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Species Specificity
- Vaccinia / veterinary
- Vaccinia / virology
- Vaccinia virus / classification
- Vaccinia virus / genetics
- Vaccinia virus / isolation & purification
- Vaccinia virus / pathogenicity
- Virulence / genetics
Citations
This article has been cited 20 times.- Silva LF, de Oliveira SAM, de Alencar Mota ALA, Gonu00e7alves VSP, de Oliveira Freitas C, Cargnelutti JF, Flores EF, de Sant'Ana FJF. Seroprevalence of bovine vaccinia in cows and its correlation with the productive profile of affected farms in Distrito Federal, Brazil.. Braz J Microbiol 2022 Mar;53(1):411-419.
- Josu00e9 da Silva Domingos I, Silva de Oliveira J, Lorene Soares Rocha K, Bretas de Oliveira D, Geessien Kroon E, Barbosa Costa G, de Souza Trindade G. Twenty Years after Bovine Vaccinia in Brazil: Where We Are and Where Are We Going?. Pathogens 2021 Mar 31;10(4).
- Silva NIO, de Oliveira JS, Kroon EG, Trindade GS, Drumond BP. Here, There, and Everywhere: The Wide Host Range and Geographic Distribution of Zoonotic Orthopoxviruses.. Viruses 2020 Dec 30;13(1).
- Ehmann R, Brandes K, Antwerpen M, Walter M, V Schlippenbach K, Stegmaier E, Essbauer S, Bugert J, Teifke JP, Meyer H. Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus.. J Gen Virol 2021 Mar;102(3).
- Lima MT, Oliveira GP, Afonso JAB, Souto RJC, de Mendonu00e7a CL, Dantas AFM, Abrahao JS, Kroon EG. An Update on the Known Host Range of the Brazilian Vaccinia Virus: An Outbreak in Buffalo Calves.. Front Microbiol 2018;9:3327.
- Kumar N, Sharma S, Barua S, Tripathi BN, Rouse BT. Virological and Immunological Outcomes of Coinfections.. Clin Microbiol Rev 2018 Oct;31(4).
- Borges IA, Reynolds MG, McCollum AM, Figueiredo PO, Ambrosio LLD, Vieira FN, Costa GB, Matos ACD, de Andrade Almeida VM, Ferreira PCP, Lobato ZIP, Dos Reis JKP, Kroon EG, Trindade GS. Serological Evidence of Orthopoxvirus Circulation Among Equids, Southeast Brazil.. Front Microbiol 2018;9:402.
- Matos ACD, Rehfeld IS, Guedes MIMC, Lobato ZIP. Bovine Vaccinia: Insights into the Disease in Cattle.. Viruses 2018 Mar 9;10(3).
- Albarnaz JD, Torres AA, Smith GL. Modulating Vaccinia Virus Immunomodulators to Improve Immunological Memory.. Viruses 2018 Feb 28;10(3).
- Calixto R, Oliveira G, Lima M, Andrade AC, Trindade GS, de Oliveira DB, Kroon EG. A Model to Detect Autochthonous Group 1 and 2 Brazilian Vaccinia virus Coinfections: Development of a qPCR Tool for Diagnosis and Pathogenesis Studies.. Viruses 2017 Dec 30;10(1).
- Lima MT, Oliveira GP, Assis FL, Bretas de Oliveira D, Vaz SM, Trindade GS, Abrahu00e3o JS, Kroon EG. Ocular Vaccinia Infection in Dairy Worker, Brazil.. Emerg Infect Dis 2018 Jan;24(1):161-162.
- Oliveira JS, Figueiredo PO, Costa GB, Assis FL, Drumond BP, da Fonseca FG, Nogueira ML, Kroon EG, Trindade GS. Vaccinia Virus Natural Infections in Brazil: The Good, the Bad, and the Ugly.. Viruses 2017 Nov 15;9(11).
- Quiner CA, Nakazawa Y. Ecological niche modeling to determine potential niche of Vaccinia virus: a case only study.. Int J Health Geogr 2017 Aug 7;16(1):28.
- Miranda JB, Borges IA, Campos SPS, Vieira FN, de u00c1zara TMF, Marques FA, Costa GB, Luis APMF, de Oliveira JS, Ferreira PCP, Bonjardim CA, da Silva SLM, Eiras u00c1E, Abrahu00e3o JS, Kroon EG, Drumond BP, Paglia AP, Trindade GS. Serologic and Molecular Evidence of Vaccinia Virus Circulation among Small Mammals from Different Biomes, Brazil.. Emerg Infect Dis 2017 Jun;23(6):931-938.
- Oliveira G, Assis F, Almeida G, Albarnaz J, Lima M, Andrade AC, Calixto R, Oliveira C, Diomedes Neto J, Trindade G, Ferreira PC, Kroon EG, Abrahu00e3o J. From lesions to viral clones: biological and molecular diversity amongst autochthonous Brazilian vaccinia virus.. Viruses 2015 Mar 16;7(3):1218-37.
- Franco-Luiz AP, Fagundes-Pereira A, Costa GB, Alves PA, Oliveira DB, Bonjardim CA, Ferreira PC, Trindade Gde S, Panei CJ, Galosi CM, Abrahu00e3o JS, Kroon EG. Spread of vaccinia virus to cattle herds, Argentina, 2011.. Emerg Infect Dis 2014 Sep;20(9):1576-8.
- Shchelkunov SN. An increasing danger of zoonotic orthopoxvirus infections.. PLoS Pathog 2013;9(12):e1003756.
- Assis FL, Borges IA, Mesquita VS, Ferreira PC, Trindade GS, Kroon EG, Abrahu00e3o JS. Vaccinia virus in household environment during bovine vaccinia outbreak, Brazil.. Emerg Infect Dis 2013 Dec;19(12):2045-7.
- Assis FL, Almeida GM, Oliveira DB, Franco-Luiz AP, Campos RK, Guedes MI, Fonseca FG, Trindade GS, Drumond BP, Kroon EG, Abrahu00e3o JS. Characterization of a new Vaccinia virus isolate reveals the C23L gene as a putative genetic marker for autochthonous Group 1 Brazilian Vaccinia virus.. PLoS One 2012;7(11):e50413.
- Assis FL, Borges IA, Ferreira PC, Bonjardim CA, Trindade Gde S, Lobato ZI, Guedes MI, Mesquita V, Kroon EG, Abrahu00e3o JS. Group 2 vaccinia virus, Brazil.. Emerg Infect Dis 2012 Dec;18(12):2035-8.