FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Am J Vet Res / Assessment of regeneration in meniscal lesions by use of mes...
American journal of veterinary research2016; 77(7); 779-788; doi: 10.2460/ajvr.77.7.779

Assessment of regeneration in meniscal lesions by use of mesenchymal stem cells derived from equine bone marrow and adipose tissue.

Abstract: OBJECTIVE To assess the ability to regenerate an equine meniscus by use of a collagen repair patch (scaffold) seeded with mesenchymal stem cells (MSCs) derived from bone marrow (BM) or adipose tissue (AT). SAMPLE 6 female Hispano-Breton horses between 4 and 7 years of age; MSCs from BM and AT were obtained for the in vitro experiment, and the horses were subsequently used for the in vivo experiment. PROCEDURES Similarities and differences between MSCs derived from BM or AT were investigated in vitro by use of cell culture. In vivo assessment involved use of a meniscus defect and implantation on a scaffold. Horses were allocated into 2 groups. In one group, defects in the medial meniscus were treated with MSCs derived from BM, whereas in the other group, defects were treated with MSCs derived from AT. Defects were created in the contralateral stifle joint but were not treated (control samples). RESULTS Both types of MSCs had universal stem cell characteristics. For in vivo testing, at 12 months after treatment, treated defects were regenerated with fibrocartilaginous tissue, whereas untreated defects were partially repaired or not repaired. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that MSCs derived from AT could be a good alternative to MSCs derived from BM for use in regenerative treatments. Results also were promising for a stem cell-based implant for use in regeneration in meniscal lesions. IMPACT FOR HUMAN MEDICINE Because of similarities in joint disease between horses and humans, these results could have applications in humans.
Get Full Text
Publication Date: 2016-06-28 PubMed ID: 27347833DOI: 10.2460/ajvr.77.7.779Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored the use of mesenchymal stem cells from equine bone marrow and adipose tissue in the regeneration of meniscus lesions in horses. The study concluded that both types of stem cells are effective in repairing damaged meniscus and that adipose tissue could be a viable alternative to bone marrow for this kind of regenerative treatment.

Objective

  • The main objective of the study was to evaluate the potential of regenerating an equine meniscus using a collagen repair patch seeded with mesenchal stem cells derived from bone marrow and adipose tissue. This was accomplished through both in vitro and in vivo experiments.

Sample and Procedures

  • The research was conducted on 6 female Hispano-Breton horses aged between 4 and 7 years. The researchers took samples of MSCs from the bone marrow and adipose tissue for in vitro experiments, before utilizing the horses in the in vivo experiment.
  • The in vitro experiment looked into the similarities and differences of MSCs derived from bone marrow and adipose tissue through cell culture.
  • For the in vivo assessment, the researchers created a meniscus defect and implanted it on a scaffold. The horses were split into two groups. One group’s defects were treated with MSCs sourced from the bone marrow, whilst the other group’s defects were treated with MSCs sourced from adipose tissue. Defects were also created in the contralateral stifle joint, which were left untreated to serve as control samples.

Results

  • Findings showed that both types of MSCs shared universal stem cell characteristics. The study found that, a year after treatment, the treated defects had regenerated with fibrocartilaginous tissue. In contrast, the untreated defects were either partially repaired or not repaired at all.
  • The study concluded that MSCs derived from adipose tissue could be an effective alternative to MSCs derived from bone marrow for regenerative treatment practices, opening up a new potential avenue for stem cell therapies.

Impact for Human Medicine

  • Given that joint disease in horses and humans share common features, the findings from this study could have potential applications in human medicine, specifically in the field of regenerative medicine and therapies associated with meniscus lesions.

Cite This Article

APA
González-Fernández ML, Pérez-Castrillo S, Sánchez-Lázaro JA, Prieto-Fernández JG, López-González ME, Lobato-Pérez S, Colaço BJ, Olivera ER, Villar-Suárez V. (2016). Assessment of regeneration in meniscal lesions by use of mesenchymal stem cells derived from equine bone marrow and adipose tissue. Am J Vet Res, 77(7), 779-788. https://doi.org/10.2460/ajvr.77.7.779

Publication

American journal of veterinary research
ISSN: 1943-5681
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 77
Issue: 7
Pages: 779-788

Researcher Affiliations

González-Fernández, Maria L
    Pérez-Castrillo, Saúl
      Sánchez-Lázaro, Jaime A
        Prieto-Fernández, Julio G
          López-González, Maria E
            Lobato-Pérez, Sandra
              Colaço, Bruno J
                Olivera, Elías R
                  Villar-Suárez, Vega

                    MeSH Terms

                    • Adipose Tissue
                    • Animals
                    • Bone Marrow Cells / cytology
                    • Bone Marrow Transplantation / veterinary
                    • Cells, Cultured
                    • Female
                    • Horses / surgery
                    • Meniscus / cytology
                    • Meniscus / surgery
                    • Mesenchymal Stem Cell Transplantation / veterinary
                    • Mesenchymal Stem Cells / cytology
                    • Regeneration
                    • Stifle

                    Citations

                    This article has been cited 19 times.
                    1. Ferreira LVO, Kamura BDC, Oliveira JPM, Chimenes ND, Carvalho M, Santos LAD, Dias-Melicio LA, Amorim RL, Amorim RM. In Vitro Transdifferentiation Potential of Equine Mesenchymal Stem Cells into Schwann-Like Cells. Stem Cells Dev 2023 Jul;32(13-14):422-432.
                      doi: 10.1089/scd.2022.0274pubmed: 37071193google scholar: lookup
                    2. Ding G, Du J, Hu X, Ao Y. Mesenchymal Stem Cells From Different Sources in Meniscus Repair and Regeneration. Front Bioeng Biotechnol 2022;10:796367.
                      doi: 10.3389/fbioe.2022.796367pubmed: 35573249google scholar: lookup
                    3. Patel JM. Impediments to Meniscal Repair: Factors at Play Beyond Vascularity. Front Bioeng Biotechnol 2022;10:843166.
                      doi: 10.3389/fbioe.2022.843166pubmed: 35299635google scholar: lookup
                    4. Rhim HC, Jeon OH, Han SB, Bae JH, Suh DW, Jang KM. Mesenchymal stem cells for enhancing biological healing after meniscal injuries. World J Stem Cells 2021 Aug 26;13(8):1005-1029.
                      doi: 10.4252/wjsc.v13.i8.1005pubmed: 34567422google scholar: lookup
                    5. Ganiev I, Alexandrova N, Aimaletdinov A, Rutland C, Malanyeva A, Rizvanov A, Zakirova E. The treatment of articular cartilage injuries with mesenchymal stem cells in different animal species. Open Vet J 2021 Jan-Mar;11(1):128-134.
                      doi: 10.4314/ovj.v11i1.19pubmed: 33898294google scholar: lookup
                    6. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals (Basel) 2021 Jan 19;11(1).
                      doi: 10.3390/ani11010234pubmed: 33477808google scholar: lookup
                    7. Lyons LP, Weinberg JB, Wittstein JR, McNulty AL. Blood in the joint: effects of hemarthrosis on meniscus health and repair techniques. Osteoarthritis Cartilage 2021 Apr;29(4):471-479.
                      doi: 10.1016/j.joca.2020.11.008pubmed: 33307179google scholar: lookup
                    8. Ribitsch I, Baptista PM, Lange-Consiglio A, Melotti L, Patruno M, Jenner F, Schnabl-Feichter E, Dutton LC, Connolly DJ, van Steenbeek FG, Dudhia J, Penning LC. Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do. Front Bioeng Biotechnol 2020;8:972.
                      doi: 10.3389/fbioe.2020.00972pubmed: 32903631google scholar: lookup
                    9. Twomey-Kozak J, Jayasuriya CT. Meniscus Repair and Regeneration: A Systematic Review from a Basic and Translational Science Perspective. Clin Sports Med 2020 Jan;39(1):125-163.
                      doi: 10.1016/j.csm.2019.08.003pubmed: 31767102google scholar: lookup
                    10. Hidalgo Perea S, Lyons LP, Nishimuta JF, Weinberg JB, McNulty AL. Evaluation of culture conditions for in vitro meniscus repair model systems using bone marrow-derived mesenchymal stem cells. Connect Tissue Res 2020 May-Jul;61(3-4):322-337.
                      doi: 10.1080/03008207.2019.1680656pubmed: 31661326google scholar: lookup
                    11. Gugjoo MB, Fazili MR, Gayas MA, Ahmad RA, Dhama K. Animal mesenchymal stem cell research in cartilage regenerative medicine - a review. Vet Q 2019 Dec;39(1):95-120.
                      doi: 10.1080/01652176.2019.1643051pubmed: 31291836google scholar: lookup
                    12. Pereira H, Fatih Cengiz I, Gomes S, Espregueira-Mendes J, Ripoll PL, Monllau JC, Reis RL, Oliveira JM. Meniscal allograft transplants and new scaffolding techniques. EFORT Open Rev 2019 Jun;4(6):279-295.
                      doi: 10.1302/2058-5241.4.180103pubmed: 31210969google scholar: lookup
                    13. Li X, Yang H, Zhang Z, Yan Z, Lv H, Zhang Y, Wu B. Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro. Mol Med Rep 2018 Nov;18(5):4477-4485.
                      doi: 10.3892/mmr.2018.9472pubmed: 30221718google scholar: lookup
                    14. Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know. Front Vet Sci 2018;5:70.
                      doi: 10.3389/fvets.2018.00070pubmed: 29713634google scholar: lookup
                    15. Zellner J, Pattappa G, Koch M, Lang S, Weber J, Pfeifer CG, Mueller MB, Kujat R, Nerlich M, Angele P. Autologous mesenchymal stem cells or meniscal cells: what is the best cell source for regenerative meniscus treatment in an early osteoarthritis situation?. Stem Cell Res Ther 2017 Oct 10;8(1):225.
                      doi: 10.1186/s13287-017-0678-zpubmed: 29017608google scholar: lookup
                    16. Yang J, Guan K, Wang JZ. Clinical study on the arthroscopic refreshing treatment of anterior cruciate ligament injury combined with stable medial meniscus ramp injury. J Musculoskelet Neuronal Interact 2017 Jun 1;17(2):108-113.
                      pubmed: 28574418
                    17. Peng X, Wu F, Hu Y, Chen Y, Wei Y, Xu W. Current advances in animal model of meniscal injury: From meniscal injury to osteoarthritis. J Orthop Translat 2025 Jan;50:388-402.
                      doi: 10.1016/j.jot.2024.11.005pubmed: 40171109google scholar: lookup
                    18. Pérez Fraile A, González-Cubero E, Martínez-Flórez S, Olivera ER, Villar-Suárez V. Regenerative Medicine Applied to Musculoskeletal Diseases in Equines: A Systematic Review. Vet Sci 2023 Nov 23;10(12).
                      doi: 10.3390/vetsci10120666pubmed: 38133217google scholar: lookup
                    19. Li H, Xiong S, Masieri FF, Monika S, Lethaus B, Savkovic V. Mesenchymal Stem Cells Isolated from Equine Hair Follicles Using a Method of Air-Liquid Interface. Stem Cell Rev Rep 2023 Nov;19(8):2943-2956.
                      doi: 10.1007/s12015-023-10619-wpubmed: 37733199google scholar: lookup
                    Subscribe to our newsletter
                    Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.