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Molecular biology reports2024; 51(1); 911; doi: 10.1007/s11033-024-09828-4

Biodiversity and probiotic potential of yeasts isolated from sumbawa horse milk.

Abstract: The microbial composition of Sumbawa Horse Milk is influenced by various factors, including environmental elements that encompass geographical location, climate, and conditions specific to Sumbawa. This study aimed to determine the biodiversity and genetic diversity of the microbiome of Sumbawa Horse Milk, with an emphasis on yeast. Methods: The diversity and group of yeast isolates were evaluated by the sequence-related amplified polymorphism (SRAP) method using ME2F-EM15R (1) and ME2F-EM12R (2) primers. Molecular identification using 18 S rRNA primers was then carried out on nine selected isolates (K_21, K_31, K_42, K_45, K_1, K_6, K_8, K_17, and K_19) to determine the type of yeast. Probiotic candidate tests were carried out on three isolates, namely K_1, K_6 and K_8. Results: Analysis with NTSYS software on the SRAP results using Primer 1 revealed the presence of two major groups, where Group I was exclusively comprised of K_45 isolate, whereas the other isolates belonged to Group II. On the other hand, analysis with NTSYS software on the SRAP analysis with Primer 2 also showed two major groups with different compositions. Group I consisted of isolates K_39, 38, 37, 36, 35, 34, 33, 31, 29, 28, 27, 26, 25, 24, 23, 22, and 21, while the remaining isolates belonged to Group II. Results of 18 S rRNA analysis demonstrated that K_17 and K_19 had 99.8 and 100% similarity, respectively, and identified as Candida humilis. K_21, K_31, and K_45 were identified as having a 100% similarity to Clavispora lusitaniae, while K_42 had a 99.8% similarity to Candida parapsilosis. Three isolates were identified as belonging to the genus Ogataea, namely Ogataea polymorpha (K_6 and K_8) and Ogataea siamensis (K_1) with similarity of 100% and 99.8%, respectively. Conclusions: These findings suggest that the three yeast have potential as probiotics.
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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Publication Date: 2024-08-16 PubMed ID: 39150593PubMed Central: 8820344DOI: 10.1007/s11033-024-09828-4Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Overview

  • This study investigates the types and genetic diversity of yeasts found in Sumbawa horse milk, focusing particularly on their diversity and potential as probiotics.

Introduction

  • The microbial makeup of Sumbawa horse milk is shaped by environmental factors such as:
    • Geographical location
    • Climate
    • Local conditions in Sumbawa
  • The goal of the research was to explore the biodiversity and genetic diversity of yeast species within the milk microbiome.

Methods

  • Yeast diversity and grouping were analyzed using the Sequence-Related Amplified Polymorphism (SRAP) technique with two primer pairs:
    • ME2F-EM15R (Primer 1)
    • ME2F-EM12R (Primer 2)
  • Molecular identification was performed on nine selected yeast isolates (K_1, K_6, K_8, K_17, K_19, K_21, K_31, K_42, K_45) using 18S rRNA gene analysis to determine yeast species.
  • Probiotic potential was evaluated in three isolates (K_1, K_6, K_8), testing their ability to serve as beneficial microbes.

Results

  • SRAP analysis with Primer 1 revealed two distinct groups:
    • Group I contained only isolate K_45.
    • Group II included all other isolates.
  • SRAP analysis with Primer 2 also divided isolates into two groups but with different composition:
    • Group I consisted of isolates K_21, K_22, K_23,… up to K_39.
    • Group II contained remaining isolates not in Group I.
  • 18S rRNA gene sequencing identified yeast species:
    • Candida humilis (K_17 and K_19) with high similarity (99.8% and 100%).
    • Clavispora lusitaniae (K_21, K_31, K_45) at 100% similarity.
    • Candida parapsilosis (K_42) with 99.8% similarity.
    • Genus Ogataea identified in three isolates:
      • Ogataea polymorpha (K_6, K_8) at 100% similarity.
      • Ogataea siamensis (K_1) at 99.8% similarity.
  • Of these, isolates K_1, K_6, and K_8 were tested for probiotic properties, showing potential for beneficial use.

Conclusions

  • The study demonstrates diverse yeast species inhabit Sumbawa horse milk, grouped distinctly by genetic markers.
  • Several species identified belong to genera known for possible probiotic traits.
  • The three isolates belonging to Ogataea genus appear promising probiotic candidates, which could be explored for health or food applications.

Cite This Article

APA
Ekawati N, Mutiara I, Hertati A, Kusdianawati , Mustopa AZ, Fatimah , Manguntungi B, Elviantari A. (2024). Biodiversity and probiotic potential of yeasts isolated from sumbawa horse milk. Mol Biol Rep, 51(1), 911. https://doi.org/10.1007/s11033-024-09828-4

Publication

Molecular biology reports
ISSN: 1573-4978
NlmUniqueID: 0403234
Country: Netherlands
Language: English
Volume: 51
Issue: 1
Pages: 911

Researcher Affiliations

Ekawati, Nurlaili
  • Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), KST Soekarno Jl Raya Bogor KM 46, Bogor, 16911, West Java, Indonesia.
Mutiara, Ilma
  • Department of Biotechnology, Faculty of Life Sciences and Technology, Sumbawa University of Technology, Sumbawa, Indonesia.
Hertati, Ai
  • Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), KST Soekarno Jl Raya Bogor KM 46, Bogor, 16911, West Java, Indonesia.
Kusdianawati,
  • Department of Biology, Faculty of Mathematics and Natural Science, Makassar State University, Makassar, Indonesia.
Mustopa, Apon Zaenal
  • Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), KST Soekarno Jl Raya Bogor KM 46, Bogor, 16911, West Java, Indonesia. azae001@brin.go.id.
Fatimah,
  • Research Center for Applied Botany, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia.
Manguntungi, Baso
  • Department of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Sulawesi Barat, Majene, Indonesia.
Elviantari, Adelia
  • Department of Biotechnology, Faculty of Life Sciences and Technology, Sumbawa University of Technology, Sumbawa, Indonesia.

MeSH Terms

  • Animals
  • Probiotics
  • Horses / microbiology
  • Yeasts / isolation & purification
  • Yeasts / genetics
  • Yeasts / classification
  • Milk / microbiology
  • Biodiversity
  • Phylogeny
  • RNA, Ribosomal, 18S / genetics
  • Genetic Variation / genetics

Grant Funding

  • RP1_Tema3_45 / DIPA PN Rumah Program Pengungkapan dan Pemanfaatan Biodiversitas Nusantara OR Hayati dan Lingkungan BRIN 2023

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Citations

This article has been cited 1 times.
  1. Zhadyra S, Tao F, Xu P. Exploring the Microbiome and Functional Metabolism of Fermented Camel Milk (Shubat) Using Metagenomics.. Foods 2025 Mar 22;14(7).
    doi: 10.3390/foods14071102pubmed: 40238218google scholar: lookup
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