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Home / Equine Research Bank / Oxid Med Cell Longev / Biomarkers of antioxidant status, inflammation, and cartilag...
Oxidative medicine and cellular longevity2012; 2012; 920932; doi: 10.1155/2012/920932

Biomarkers of antioxidant status, inflammation, and cartilage metabolism are affected by acute intense exercise but not superoxide dismutase supplementation in horses.

Abstract: Objectives were to evaluate effects of (1) repetitive arthrocentesis on biomarkers of inflammation (prostaglandin E(2), PGE(2)) and aggrecan synthesis (chondroitin sulfate-846; CS) in synovial fluid (SF); (2) exercise and superoxide dismutase (SOD) supplementation on biomarkers of inflammation, antioxidant status, and aggrecan synthesis, in horses. Preliminary trial. Standardbreds underwent four arthrocentesis procedures within 48 h and exhibited elevated CS and no changes in PGE(2). Exercise trial. this randomized crossover design used twelve Standardbred mares which received either treatment (3000 IU d(-1) oral SOD powder) or placebo (cellulose powder) for 6 wks which culminated with them running a repeated sprint exercise test (RSET). Samples were collected before (PRE), during (PEAK), and following exercise (POST). Exercise resulted in increased (P < 0.05) antioxidant defenses including erythrocyte SOD, total glutathione, glutathione peroxidase, gene transcripts for interferon-gamma, interleukin-10, and interleukin-1β in blood, and decreased plasma nitric oxide. Exercise increased (P < 0.05) SF CS and adjusted-PGE(2), and higher (P < 0.05) CS and PGE(2) were found in hock versus carpus joints. No treatment effects were detected. Results suggest normal adaptive responses likely due to exercise-induced tissue microdamage and oxidative stress. Additional research is needed to identify benefit(s) of SOD supplementation in horses.
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Publication Date: 2012-08-08 PubMed ID: 22919442PubMed Central: PMC3423952DOI: 10.1155/2012/920932Google Scholar: Lookup
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  • Journal Article
  • Randomized Controlled Trial
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study conducted on horses to understand the influence of intense exercise and superoxide dismutase (SOD) supplementation on biomarkers related to inflammation, antioxidant status, and cartilage metabolism. The study did not find any significant effects due to SOD supplementation.

Research Methodology

  • The study was divided into two subsets – a preliminary trial and an exercise trial. The preliminary trial involved four arthrocentesis procedures within 48 hours to establish baseline biomarker levels. The exercise trial, using a randomised crossover design, involved twelve Standardbred mares receiving either a treatment (3000 IU d(-1) oral SOD powder) or a placebo (cellulose powder). The six-week trial ended with the horses undertaking a repeated sprint exercise test (RSET).
  • Blood and synovial fluid samples were collected before (PRE), during (PEAK), and after the exercise (POST) to examine for changes in biomarkers of inflammation, antioxidant status, and aggrecan synthesis.

Results

  • After the RSET, the horses showed an increase in antioxidant defenses including erythrocyte SOD, total glutathione, glutathione peroxidase, and gene transcripts for interferon-gamma, interleukin-10, and interleukin-1β in blood. There was also a decrease in plasma nitric oxide levels.
  • The horses also demonstrated a rise in synovial fluid (SF) Chondroitin sulfate-846 (CS) and prostaglandin E(2), with higher levels of CS and PGE(2) in the hock joints compared to the carpus joints. These biomarkers indicate cartilage metabolism and inflammation, respectively.
  • No significant treatment effects were detected, implying that the SOD supplementation did not lead to noticeable changes in these biomarkers.

Conclusion

  • The influence seen on biomarkers was due to the intense exercise, believed to cause tissue microdamage and oxidative stress, leading to adaptive responses in the body.
  • The study concluded that more research is needed to identify any benefits of SOD supplementation in horses as it did not show any marked influence on the biomarkers studied.

Cite This Article

APA
Lamprecht ED, Williams CA. (2012). Biomarkers of antioxidant status, inflammation, and cartilage metabolism are affected by acute intense exercise but not superoxide dismutase supplementation in horses. Oxid Med Cell Longev, 2012, 920932. https://doi.org/10.1155/2012/920932

Publication

Oxidative medicine and cellular longevity
ISSN: 1942-0994
NlmUniqueID: 101479826
Country: United States
Language: English
Volume: 2012
Pages: 920932
PII: 920932

Researcher Affiliations

Lamprecht, Emily D
  • Department of Animal Sciences, Rutgers the State University, New Brunswick, NJ 08901-8525, USA. emily lamprecht@cargill.com
Williams, Carey A

    MeSH Terms

    • Animals
    • Antioxidants / metabolism
    • Biomarkers / blood
    • Cartilage / drug effects
    • Cartilage / metabolism
    • Dietary Supplements
    • Horses / blood
    • Inflammation / blood
    • Inflammation / veterinary
    • Joints / metabolism
    • Joints / pathology
    • Physical Conditioning, Animal
    • Superoxide Dismutase / administration & dosage
    • Superoxide Dismutase / pharmacology

    References

    This article includes 118 references
    1. Auer DE, Ng JC, Hrdlicka J, Seawright AA. The elimination of injected superoxide dismutase from synovial fluid of the horse.. Aust Vet J 1989 Apr;66(4):117-9.
      pubmed: 2730476doi: 10.1111/j.1751-0813.1989.tb09763.xgoogle scholar: lookup
    2. Bertone AL, Palmer JL, Jones J. Synovial fluid cytokines and eicosanoids as markers of joint disease in horses.. Vet Surg 2001 Nov-Dec;30(6):528-38.
      pubmed: 11704948doi: 10.1053/jvet.2001.28430google scholar: lookup
    3. Petersen HH, Nielsen JP, Heegaard PM. Application of acute phase protein measurements in veterinary clinical chemistry.. Vet Res 2004 Mar-Apr;35(2):163-87.
      pubmed: 15099494doi: 10.1051/vetres:2004002google scholar: lookup
    4. Firth EC. The response of bone, articular cartilage and tendon to exercise in the horse.. J Anat 2006 Apr;208(4):513-26.
      pmc: PMC2100207pubmed: 16637875doi: 10.1111/j.1469-7580.2006.00547.xgoogle scholar: lookup
    5. Streltsova JM, McKeever KH, Liburt NR. Effect of orange peel and black tea extracts on markers of performance and cytokine markers of inflammation.. Equine and Comparative Exercise Physiology 2006;2006:121–130.
    6. Lamprecht ED, Bagnell CA, Williams CA. Inflammatory responses to three modes of intense exercise in Standardbred mares-A pilot study.. Comparative Exercise Physiology 2009;5:115–125.
    7. Lang JD, McArdle PJ, O'Reilly PJ, Matalon S. Oxidant-antioxidant balance in acute lung injury.. Chest 2002 Dec;122(6 Suppl):314S-320S.
      pubmed: 12475808doi: 10.1378/chest.122.6_suppl.314sgoogle scholar: lookup
    8. Ishida N, Hobo S, Takahashi T, Nanbo Y, Sato F, Hasegawa T, Mukoyama H. Chronological changes in superoxide-scavenging ability and lipid peroxide concentration of equine serum due to stress from exercise and transport.. Equine Vet J Suppl 1999 Jul;(30):430-3.
      pubmed: 10659294doi: 10.1111/j.2042-3306.1999.tb05260.xgoogle scholar: lookup
    9. White A, Estrada M, Walker K, Wisnia P, Filgueira G, Valdés F, Araneda O, Behn C, Martínez R. Role of exercise and ascorbate on plasma antioxidant capacity in thoroughbred race horses.. Comp Biochem Physiol A Mol Integr Physiol 2001 Jan;128(1):99-104.
      pubmed: 11137442doi: 10.1016/s1095-6433(00)00286-5google scholar: lookup
    10. Marlin DJ, Fenn K, Smith N, Deaton CD, Roberts CA, Harris PA, Dunster C, Kelly FJ. Changes in circulatory antioxidant status in horses during prolonged exercise.. J Nutr 2002 Jun;132(6 Suppl 2):1622S-7S.
      pubmed: 12042474doi: 10.1093/jn/132.6.1622sgoogle scholar: lookup
    11. Williams CA, Kronfeld DS, Hess TM, Saker KE, Harris PA. Lipoic acid and vitamin E supplementation to horses diminishes endurance exercise induced oxidative stress, muscle enzyme leakage, and apoptosis.. Page 105 in The Elite Race and Endurance Horse. In: Lindner A, editor. Proceedings of the Equine Sports Medicine and Science; 2004; Oslo, Norway.
    12. Kinnunen S, Hyyppä S, Lehmuskero A, Oksala N, Mäenpää P, Hänninen O, Atalay M. Oxygen radical absorbance capacity (ORAC) and exercise-induced oxidative stress in trotters.. Eur J Appl Physiol 2005 Dec;95(5-6):550-6.
      pubmed: 16136323doi: 10.1007/s00421-005-0034-3google scholar: lookup
    13. Shek PN, Sabiston BH, Buguet A, Radomski MW. Strenuous exercise and immunological changes: a multiple-time-point analysis of leukocyte subsets, CD4/CD8 ratio, immunoglobulin production and NK cell response.. Int J Sports Med 1995 Oct;16(7):466-74.
      pubmed: 8550256doi: 10.1055/s-2007-973039google scholar: lookup
    14. Brenner IK, Natale VM, Vasiliou P, Moldoveanu AI, Shek PN, Shephard RJ. Impact of three different types of exercise on components of the inflammatory response.. Eur J Appl Physiol Occup Physiol 1999 Oct;80(5):452-60.
      pubmed: 10502079doi: 10.1007/s004210050617google scholar: lookup
    15. Rossdale PD, Hopes R, Digby NJ, offord K. Epidemiological study of wastage among racehorses 1982 and 1983.. Vet Rec 1985 Jan 19;116(3):66-9.
      pubmed: 3976145doi: 10.1136/vr.116.3.66google scholar: lookup
    16. Kidd JA, Fuller C, Barr ARS. Osteoarthritis in the horse.. Equine Veterinary Education 2001;13(3):160–168.
    17. USDA. Lameness and Laminitis in U.S. Horses.. USDA:APHIS:VS, CEAH, National Animal Health Monitoring System. #N348.1001 USDA, Fort Collins, Colo, USA, 2001.
    18. Dyson PK, Jackson BF, Pfeiffer DU, Price JS. Days lost from training by two- and three-year-old Thoroughbred horses: a survey of seven UK training yards.. Equine Vet J 2008 Nov;40(7):650-7.
      pubmed: 19165934doi: 10.2746/042516408x363242google scholar: lookup
    19. von Rechenberg B, McIlwraith CW, Akens MK, Frisbie DD, Leutenegger C, Auer JA. Spontaneous production of nitric oxide (NO), prostaglandin (PGE2) and neutral metalloproteinases (NMPs) in media of explant cultures of equine synovial membrane and articular cartilage from normal and osteoarthritic joints.. Equine Vet J 2000 Mar;32(2):140-50.
      pubmed: 10743970doi: 10.2746/042516400777591598google scholar: lookup
    20. Tchetina EV, Di Battista JA, Zukor DJ, Antoniou J, Poole AR. Prostaglandin PGE2 at very low concentrations suppresses collagen cleavage in cultured human osteoarthritic articular cartilage: this involves a decrease in expression of proinflammatory genes, collagenases and COL10A1, a gene linked to chondrocyte hypertrophy.. Arthritis Res Ther 2007;9(4):R75.
      pmc: PMC2206385pubmed: 17683641doi: 10.1186/ar2273google scholar: lookup
    21. McIlwraith CW. Use of synovial fluid and serum biomarkers in equine bone and joint disease: a review.. Equine Vet J 2005 Sep;37(5):473-82.
      pubmed: 16163952doi: 10.2746/042516405774480102google scholar: lookup
    22. Frisbie DD, Al-Sobayil F, Billinghurst RC, Kawcak CE, McIlwraith CW. Changes in synovial fluid and serum biomarkers with exercise and early osteoarthritis in horses.. Osteoarthritis Cartilage 2008 Oct;16(10):1196-204.
      pubmed: 18442931doi: 10.1016/j.joca.2008.03.008google scholar: lookup
    23. Poole AR, Ionescu M, Swan A, Dieppe PA. Changes in cartilage metabolism in arthritis are reflected by altered serum and synovial fluid levels of the cartilage proteoglycan aggrecan. Implications for pathogenesis.. J Clin Invest 1994 Jul;94(1):25-33.
      pmc: PMC296278pubmed: 7518830doi: 10.1172/jci117314google scholar: lookup
    24. Frisbie DD, Ray CS, Ionescu M, Poole AR, Chapman PL, McIlwraith CW. Measurement of synovial fluid and serum concentrations of the 846 epitope of chondroitin sulfate and of carboxy propeptides of type II procollagen for diagnosis of osteochondral fragmentation in horses.. Am J Vet Res 1999 Mar;60(3):306-9.
      pubmed: 10188811
    25. Fortier LA. Systemic therapies for joint disease in horses.. Vet Clin North Am Equine Pract 2005 Dec;21(3):547-57, v.
      pubmed: 16297720doi: 10.1016/j.cveq.2005.07.002google scholar: lookup
    26. Nelson SK, Bose SK, Grunwald GK, Myhill P, McCord JM. The induction of human superoxide dismutase and catalase in vivo: a fundamentally new approach to antioxidant therapy.. Free Radic Biol Med 2006 Jan 15;40(2):341-7.
      pubmed: 16413416doi: 10.1016/j.freeradbiomed.2005.08.043google scholar: lookup
    27. Cuzzocrea S, Mazzon E, Dugo L, Caputi AP, Riley DP, Salvemini D. Protective effects of M40403, a superoxide dismutase mimetic, in a rodent model of colitis.. Eur J Pharmacol 2001 Nov 30;432(1):79-89.
      pubmed: 11734191doi: 10.1016/s0014-2999(01)01427-3google scholar: lookup
    28. Masini E, Cuzzocrea S, Mazzon E, Marzocca C, Mannaioni PF, Salvemini D. Protective effects of M40403, a selective superoxide dismutase mimetic, in myocardial ischaemia and reperfusion injury in vivo.. Br J Pharmacol 2002 Jul;136(6):905-17.
      pmc: PMC1573411pubmed: 12110615doi: 10.1038/sj.bjp.0704774google scholar: lookup
    29. Vouldoukis I, Conti M, Krauss P, Kamaté C, Blazquez S, Tefit M, Mazier D, Calenda A, Dugas B. Supplementation with gliadin-combined plant superoxide dismutase extract promotes antioxidant defences and protects against oxidative stress.. Phytother Res 2004 Dec;18(12):957-62.
      pubmed: 15742357doi: 10.1002/ptr.1542google scholar: lookup
    30. Arent SM, Davitt P, Golem DL, Williams CA, McKeever KH, Jaouhari C. The effects of a post-workout nutraceutical drink on body composition, performance and hormonal and biochemical responses in Division I college football players.. Comparative Exercise Physiology 2009;6:73–80.
    31. Arent SM, Pellegrino JK, Williams CA, Difabio DA, Greenwood JC. Nutritional supplementation, performance, and oxidative stress in college soccer players.. J Strength Cond Res 2010 Apr;24(4):1117-24.
      pubmed: 20300015doi: 10.1519/jsc.0b013e3181cb70b8google scholar: lookup
    32. Petelin M, Pavlica Z, Ivanusa T, Sentjurc M, Skaleric U. Local delivery of liposome-encapsulated superoxide dismutase and catalase suppress periodontal inflammation in beagles.. J Clin Periodontol 2000 Dec;27(12):918-25.
      pubmed: 11140559doi: 10.1034/j.1600-051x.2000.027012918.xgoogle scholar: lookup
    33. Huber W. Orgotein--(bovine Cu-Zn superoxide dismutase), an anti-inflammatory protein drug: discovery, toxicology and pharmacology.. Eur J Rheumatol Inflamm 1981;4(2):173-82.
      pubmed: 7343319
    34. Auer DE, Ng JC, Seawright AA. Effect of palosein (superoxide dismutase) and catalase upon oxygen derived free radical induced degradation of equine synovial fluid.. Equine Vet J 1990 Jan;22(1):13-7.
      pubmed: 2298185doi: 10.1111/j.2042-3306.1990.tb04195.xgoogle scholar: lookup
    35. American Association of Equine Practitioners. Guide for Veterinary Service and Judging of Equestrian Events.. 4th edition 1991.
    36. Henneke DR, Potter GD, Kreider JL, Yeates BF. Relationship between condition score, physical measurements and body fat percentage in mares.. Equine Vet J 1983 Oct;15(4):371-2.
      pubmed: 6641685doi: 10.1111/j.2042-3306.1983.tb01826.xgoogle scholar: lookup
    37. Westervelt RG, Stouffer JR, Hintz HF, Schryver HF. Estimating fatness in horses and ponies.. Journal of Animal Science 1976;43:781–785.
    38. Au RY, Al-Talib TK, Au AY, Phan PV, Frondoza CG. Avocado soybean unsaponifiables (ASU) suppress TNF-alpha, IL-1beta, COX-2, iNOS gene expression, and prostaglandin E2 and nitric oxide production in articular chondrocytes and monocyte/macrophages.. Osteoarthritis Cartilage 2007 Nov;15(11):1249-55.
      pubmed: 17845860doi: 10.1016/j.joca.2007.07.009google scholar: lookup
    39. Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.. Anal Biochem 1971 Nov;44(1):276-87.
      pubmed: 4943714doi: 10.1016/0003-2697(71)90370-8google scholar: lookup
    40. Graham-Thiers PM, Kronfeld DS, Kline KA, Sklan DJ, Harris PA. Dietary protein and fat effects on protein status in arabian horses during interval training and repeated sprints.. Journal of Equine Veterinary Science 2003;23(12):554–559.
    41. Wilson JA, Kronfeld DS, Gay LS, Williams JH, Wilson TM, Lindinger MI. Sarcoplasmic reticulum responses to repeated sprints are affected by conditioning of horses.. J Anim Sci 1998 Dec;76(12):3065-71.
      pubmed: 9928611doi: 10.2527/1998.76123065xgoogle scholar: lookup
    42. Hasegawa K, Wakino S, Tatematsu S, Yoshioka K, Homma K, Sugano N, Kimoto M, Hayashi K, Itoh H. Role of asymmetric dimethylarginine in vascular injury in transgenic mice overexpressing dimethylarginie dimethylaminohydrolase 2.. Circ Res 2007 Jul 20;101(2):e2-10.
      pubmed: 17601800doi: 10.1161/circresaha.107.156901google scholar: lookup
    43. Cybulski RJ Jr, Sanz P, Alem F, Stibitz S, Bull RL, O'Brien AD. Four superoxide dismutases contribute to Bacillus anthracis virulence and provide spores with redundant protection from oxidative stress.. Infect Immun 2009 Jan;77(1):274-85.
      pmc: PMC2612278pubmed: 18955476doi: 10.1128/iai.00515-08google scholar: lookup
    44. Qin Z, Karabiyikoglu M, Hua Y, Silbergleit R, He Y, Keep RF, Xi G. Hyperbaric oxygen-induced attenuation of hemorrhagic transformation after experimental focal transient cerebral ischemia.. Stroke 2007 Apr;38(4):1362-7.
      pubmed: 17322079doi: 10.1161/01.str.0000259660.62865.ebgoogle scholar: lookup
    45. Ursini F, Maiorino M, Brigelius-Flohé R, Aumann KD, Roveri A, Schomburg D, Flohé L. Diversity of glutathione peroxidases.. Methods Enzymol 1995;252:38-53.
      pubmed: 7476373doi: 10.1016/0076-6879(95)52007-4google scholar: lookup
    46. Richie JP Jr, Skowronski L, Abraham P, Leutzinger Y. Blood glutathione concentrations in a large-scale human study.. Clin Chem 1996 Jan;42(1):64-70.
      pubmed: 8565235
    47. Vick MM, Adams AA, Murphy BA, Sessions DR, Horohov DW, Cook RF, Shelton BJ, Fitzgerald BP. Relationships among inflammatory cytokines, obesity, and insulin sensitivity in the horse.. J Anim Sci 2007 May;85(5):1144-55.
      pubmed: 17264235doi: 10.2527/jas.2006-673google scholar: lookup
    48. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.. Methods 2001 Dec;25(4):402-8.
      pubmed: 11846609doi: 10.1006/meth.2001.1262google scholar: lookup
    49. Ainsworth DM, Appleton JA, Eicker SW, Luce R, Julia Flaminio M, Antczak DF. The effect of strenuous exercise on mRNA concentrations of interleukin-12, interferon-gamma and interleukin-4 in equine pulmonary and peripheral blood mononuclear cells.. Vet Immunol Immunopathol 2003 Jan 10;91(1):61-71.
      pubmed: 12507851doi: 10.1016/s0165-2427(02)00274-xgoogle scholar: lookup
    50. Ramakers C, Ruijter JM, Deprez RH, Moorman AF. Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data.. Neurosci Lett 2003 Mar 13;339(1):62-6.
      pubmed: 12618301doi: 10.1016/s0304-3940(02)01423-4google scholar: lookup
    51. van den Boom R, van de Lest CH, Bull S, Brama RA, van Weeren PR, Barneveld A. Influence of repeated arthrocentesis and exercise on synovial fluid concentrations of nitric oxide, prostaglandin E2 and glycosaminoglycans in healthy equine joints.. Equine Vet J 2005 May;37(3):250-6.
      pubmed: 15892235doi: 10.2746/0425164054530740google scholar: lookup
    52. Brama PA, van den Boom R, DeGroott J, Kiers GH, van Weeren PR. Collagenase-1 (MMP-1) activity in equine synovial fluid: influence of age, joint pathology, exercise and repeated arthrocentesis.. Equine Vet J 2004 Jan;36(1):34-40.
      pubmed: 14756369doi: 10.2746/0425164044864705google scholar: lookup
    53. van den Boom R, Brama PA, Kiers GH, DeGroot J, Barneveld A, van Weeren RR. The influence of repeated arthrocentesis and exercise on matrix metalloproteinase and tumour necrosis factor alpha activities in normal equine joints.. Equine Vet J 2004 Mar;36(2):155-9.
      pubmed: 15038439doi: 10.2746/0425164044868602google scholar: lookup
    54. Robion FC, Doizé B, Bouré L, Marcoux M, Ionescu M, Reiner A, Poole AR, Laverty S. Use of synovial fluid markers of cartilage synthesis and turnover to study effects of repeated intra-articular administration of methylprednisolone acetate on articular cartilage in vivo.. J Orthop Res 2001 Mar;19(2):250-8.
      pubmed: 11347698doi: 10.1016/s0736-0266(00)90008-1google scholar: lookup
    55. Szucsik A, Baliskonis V, McKeever KH. Effect of seven common supplements on plasma electrolyte and total carbon dioxide concentration and strong ion difference in Standardbred horses subjected to a simulated race test.. Equine and Comparative Exercise Physiology 2006;3:37–44.
    56. Williams CA, Gordon ME, Betros CL, McKeever KH. Apoptosis and antioxidant status are influenced by age and exercise training in horses.. J Anim Sci 2008 Mar;86(3):576-83.
      pubmed: 18156356doi: 10.2527/jas.2007-0585google scholar: lookup
    57. Padalino B, Rubino G, Centoducati P, Petazzi F. Training versus overtraining: evaluation of two protocols.. Journal of Equine Veterinary Science 2007;27(1):28–31.
    58. Harris PA. Musculoskeletal disease.. In: Reed SM, Bayly WM, editors. Equine Internal Medicine. WB Saunders; 1998. pp. 375–397.
    59. Frankiewicz-Jóźko A, Szarska E. Anti-oxidant level to exercise in the blood of endurance horses.. Biology of Sport 2000;17(3):217–227.
    60. Williams CA, Kronfeld DS, Hess TM. Comparison of oxidative stress and antioxidant status in endurance horses in three 80-km races.. Equine and Comparative Exercise Physiology 2004;2:153–157.
    61. McBride JM, Kraemer WJ. Effect of vitamin E status on lipid peroxidation in exercised horses.. The Journal of Strength & Conditioning Research 1999;13:175–183.
    62. Moldoveanu AI, Shephard RJ, Shek PN. The cytokine response to physical activity and training.. Sports Med 2001 Feb;31(2):115-44.
      pmc: PMC7101891pubmed: 11227979doi: 10.2165/00007256-200131020-00004google scholar: lookup
    63. Haddad JJ, Fahlman CS. Redox- and oxidant-mediated regulation of interleukin-10: an anti-inflammatory, antioxidant cytokine?. Biochem Biophys Res Commun 2002 Sep 20;297(2):163-76.
      pubmed: 12237098doi: 10.1016/s0006-291x(02)02094-6google scholar: lookup
    64. Oberholzer A, Oberholzer C, Moldawer LL. Interleukin-10: A complex role in the pathogenesis of sepsis syndromes and its potential as an anti-inflammatory drug.. Crit Care Med 2002 Jan;30(1 Supp):S58-S63.
      pubmed: 11839946
    65. Pezzilli R, Billi P, Miniero R, Barakat B. Serum interleukin-10 in human acute pancreatitis.. Dig Dis Sci 1997 Jul;42(7):1469-72.
      pubmed: 9246048doi: 10.1023/a:1018814710291google scholar: lookup
    66. Gunnett CA, Heistad DD, Berg DJ, Faraci FM. IL-10 deficiency increases superoxide and endothelial dysfunction during inflammation.. Am J Physiol Heart Circ Physiol 2000 Oct;279(4):H1555-62.
      pubmed: 11009441doi: 10.1152/ajpheart.2000.279.4.h1555google scholar: lookup
    67. Gougerot-Podicalo MA, Elbim C, Chollet-Martin S. [Modulation of the oxidative burst of human neutrophils by pro- and anti-inflammatory cytokines].. Pathol Biol (Paris) 1996 Jan;44(1):36-41.
      pubmed: 8734298
    68. Chen S, Gong J, Liu F, Mohammed U. Naturally occurring polyphenolic antioxidants modulate IgE-mediated mast cell activation.. Immunology 2000 Aug;100(4):471-80.
      pmc: PMC2327042pubmed: 10929074doi: 10.1046/j.1365-2567.2000.00045.xgoogle scholar: lookup
    69. Hong Y, Hong S, Chang YH, Cho SH. Influence of an orally effective superoxide dismutase (Glisodin) on strenuous exercise-induced changes of blood antioxidant enzymes and plasma lactate.. Proceedings of the American Association for Clinical Chemistry. National Meeting-Poster 2004; Los Angeles, Calif, USA.
    70. Bloomer RJ. Effect of exercise on oxidative stress biomarkers.. Adv Clin Chem 2008;46:1-50.
      pubmed: 19004186doi: 10.1016/s0065-2423(08)00401-0google scholar: lookup
    71. Andersson H, Karlsen A, Blomhoff R, Raastad T, Kadi F. Plasma antioxidant responses and oxidative stress following a soccer game in elite female players.. Scand J Med Sci Sports 2010 Aug;20(4):600-8.
      pubmed: 19706000doi: 10.1111/j.1600-0838.2009.00987.xgoogle scholar: lookup
    72. Mills PC, Smith NC, Casas I, Harris P, Harris RC, Marlin DJ. Effects of exercise intensity and environmental stress on indices of oxidative stress and iron homeostasis during exercise in the horse.. Eur J Appl Physiol Occup Physiol 1996;74(1-2):60-6.
      pubmed: 8891501doi: 10.1007/bf00376495google scholar: lookup
    73. Chiaradia E, Avellini L, Rueca F, Spaterna A, Porciello F, Antonioni MT, Gaiti A. Physical exercise, oxidative stress and muscle damage in racehorses.. Comp Biochem Physiol B Biochem Mol Biol 1998 Apr;119(4):833-6.
      pubmed: 9787774doi: 10.1016/s0305-0491(98)10001-9google scholar: lookup
    74. Williams CA, Carlucci SA. Oral vitamin E supplementation on oxidative stress, vitamin and antioxidant status in intensely exercised horses.. Equine Vet J Suppl 2006 Aug;(36):617-21.
      pubmed: 17402493doi: 10.1111/j.2042-3306.2006.tb05614.xgoogle scholar: lookup
    75. Donovan DC, Jackson CA, Colahan PT, Norton NN, Clapper JL, Moore JN, Hurley DJ. Assessment of exercise-induced alterations in neutrophil function in horses.. Am J Vet Res 2007 Nov;68(11):1198-204.
      pubmed: 17975974doi: 10.2460/ajvr.68.11.1198google scholar: lookup
    76. Ji LL, Fu R. Responses of glutathione system and antioxidant enzymes to exhaustive exercise and hydroperoxide.. J Appl Physiol (1985) 1992 Feb;72(2):549-54.
      pubmed: 1559931doi: 10.1152/jappl.1992.72.2.549google scholar: lookup
    77. Sahlin K, Ekberg K, Cizinsky S. Changes in plasma hypoxanthine and free radical markers during exercise in man.. Acta Physiol Scand 1991 Jun;142(2):275-81.
      pubmed: 1877376doi: 10.1111/j.1748-1716.1991.tb09157.xgoogle scholar: lookup
    78. Ono K, Inui K, Hasegawa T, Matsuki N, Watanabe H, Takagi S, Hasegawa A, Tomoda I. The changes of antioxidative enzyme activities in equine erythrocytes following exercise.. Nihon Juigaku Zasshi 1990 Aug;52(4):759-65.
      pubmed: 2391775doi: 10.1292/jvms1939.52.759google scholar: lookup
    79. Hargreaves BJ, Kronfeld DS, Waldron JN, Lopes MA, Gay LS, Saker KE, Cooper WL, Sklan DJ, Harris PA. Antioxidant status of horses during two 80-km endurance races.. J Nutr 2002 Jun;132(6 Suppl 2):1781S-3S.
      pubmed: 12042527doi: 10.1093/jn/132.6.1781sgoogle scholar: lookup
    80. Niess AM, Dickhuth HH, Northoff H, Fehrenbach E. Free radicals and oxidative stress in exercise--immunological aspects.. Exerc Immunol Rev 1999;5:22-56.
      pubmed: 10519061
    81. Donovan DC, Jackson CA, Colahan PT, Norton N, Hurley DJ. Exercise-induced alterations in pro-inflammatory cytokines and prostaglandin F2alpha in horses.. Vet Immunol Immunopathol 2007 Aug 15;118(3-4):263-9.
      pubmed: 17617470doi: 10.1016/j.vetimm.2007.05.015google scholar: lookup
    82. Sureda A, Tauler P, Aguiló A, Fuentespina E, Córdova A, Tur JA, Pons A. Blood cell NO synthesis in response to exercise.. Nitric Oxide 2006 Aug;15(1):5-12.
      pubmed: 16376593doi: 10.1016/j.niox.2005.11.004google scholar: lookup
    83. Lane Smith R, Trindade MC, Ikenoue T, Mohtai M, Das P, Carter DR, Goodman SB, Schurman DJ. Effects of shear stress on articular chondrocyte metabolism.. Biorheology 2000;37(1-2):95-107.
      pubmed: 10912182
    84. Smith RL, Donlon BS, Gupta MK, Mohtai M, Das P, Carter DR, Cooke J, Gibbons G, Hutchinson N, Schurman DJ. Effects of fluid-induced shear on articular chondrocyte morphology and metabolism in vitro.. J Orthop Res 1995 Nov;13(6):824-31.
      pubmed: 8544017doi: 10.1002/jor.1100130604google scholar: lookup
    85. Dimock AN, Siciliano PD, McIlwraith CW. Evidence supporting an increased presence of reactive oxygen species in the diseased equine joint.. Equine Vet J 2000 Sep;32(5):439-43.
      pubmed: 11037267doi: 10.2746/042516400777591129google scholar: lookup
    86. Greenwald RA, Moy WW. Inhibition of collagen gelation by action of the superoxide radical.. Arthritis Rheum 1979 Mar;22(3):251-9.
      pubmed: 217393doi: 10.1002/art.1780220307google scholar: lookup
    87. May S, Hooke R, Peremans K. Prostaglandin E2 in equine joint disease.. Vlaams Diergeneeskundig Tijdschrift 1994;63(6):187–191.
    88. Chan PS, Caron JP, Rosa GJ, Orth MW. Glucosamine and chondroitin sulfate regulate gene expression and synthesis of nitric oxide and prostaglandin E(2) in articular cartilage explants.. Osteoarthritis Cartilage 2005 May;13(5):387-94.
      pubmed: 15882562doi: 10.1016/j.joca.2005.01.003google scholar: lookup
    89. Teitz CC, Chrisman OD. The effect of salicylate and chloroquine on prostaglandin-induced articular damage in the rabbit knee.. Clin Orthop Relat Res 1975 May;(108):264-74.
      pubmed: 49235doi: 10.1097/00003086-197505000-00039google scholar: lookup
    90. Lippiello L, Yamamoto K, Robinson D, Mankin HJ. Involvement of prostaglandins from rheumatoid synovium in inhibition of articular cartilage metabolism.. Arthritis Rheum 1978 Nov-Dec;21(8):909-17.
      pubmed: 737014doi: 10.1002/art.1780210807google scholar: lookup
    91. Palmoski MJ, Brandt KD. Effects of static and cyclic compressive loading on articular cartilage plugs in vitro.. Arthritis Rheum 1984 Jun;27(6):675-81.
      pubmed: 6732884doi: 10.1002/art.1780270611google scholar: lookup
    92. Sah RL, Kim YJ, Doong JY, Grodzinsky AJ, Plaas AH, Sandy JD. Biosynthetic response of cartilage explants to dynamic compression.. J Orthop Res 1989;7(5):619-36.
      pubmed: 2760736doi: 10.1002/jor.1100070502google scholar: lookup
    93. Parkkinen JJ, Ikonen J, Lammi MJ, Laakkonen J, Tammi M, Helminen HJ. Effects of cyclic hydrostatic pressure on proteoglycan synthesis in cultured chondrocytes and articular cartilage explants.. Arch Biochem Biophys 1993 Jan;300(1):458-65.
      pubmed: 8424680doi: 10.1006/abbi.1993.1062google scholar: lookup
    94. Buschmann MD, Kim YJ, Wong M, Frank E, Hunziker EB, Grodzinsky AJ. Stimulation of aggrecan synthesis in cartilage explants by cyclic loading is localized to regions of high interstitial fluid flow.. Arch Biochem Biophys 1999 Jun 1;366(1):1-7.
      pubmed: 10334856doi: 10.1006/abbi.1999.1197google scholar: lookup
    95. Frank EH, Jin M, Loening AM, Levenston ME, Grodzinsky AJ. A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants.. J Biomech 2000 Nov;33(11):1523-7.
      pubmed: 10940414doi: 10.1016/s0021-9290(00)00100-7google scholar: lookup
    96. Palmer JL, Bertone AL, Malemud CJ, Carter BG, Papay RS, Mansour J. Site-specific proteoglycan characteristics of third carpal articular cartilage in exercised and nonexercised horses.. Am J Vet Res 1995 Dec;56(12):1570-6.
      pubmed: 8599516
    97. Little CB, Ghosh P, Rose R. The effect of strenuous versus moderate exercise on the metabolism of proteoglycans in articular cartilage from different weight-bearing regions of the equine third carpal bone.. Osteoarthritis Cartilage 1997 May;5(3):161-72.
      pubmed: 9219679doi: 10.1016/s1063-4584(97)80011-8google scholar: lookup
    98. Okumura M, Kim GH, Tagami M, Haramaki S, Fujinaga T. Serum keratan sulphate as a cartilage metabolic marker in horses: the effect of exercise.. J Vet Med A Physiol Pathol Clin Med 2002 May;49(4):195-7.
      pubmed: 12069261doi: 10.1046/j.1439-0442.2002.00434.xgoogle scholar: lookup
    99. Das P, Schurman DJ, Smith RL. Nitric oxide and G proteins mediate the response of bovine articular chondrocytes to fluid-induced shear.. J Orthop Res 1997 Jan;15(1):87-93.
      pubmed: 9066531doi: 10.1002/jor.1100150113google scholar: lookup
    100. Grøndahl AM, Dolvik NI. Heritability estimations of osteochondrosis in the tibiotarsal joint and of bony fragments in the palmar/plantar portion of the metacarpo- and metatarsophalangeal joints of horses.. J Am Vet Med Assoc 1993 Jul 1;203(1):101-4.
      pubmed: 8407439
    101. Webb CB, Lehman TL, McCord KW. Effects of an oral superoxide dismutase enzyme supplementation on indices of oxidative stress, proviral load, and CD4:CD8 ratios in asymptomatic FIV-infected cats.. J Feline Med Surg 2008 Oct;10(5):423-30.
      pubmed: 18387839doi: 10.1016/j.jfms.2008.01.008google scholar: lookup
    102. Vouldoukis I, Lacan D, Kamate C, Coste P, Calenda A, Mazier D, Conti M, Dugas B. Antioxidant and anti-inflammatory properties of a Cucumis melo LC. extract rich in superoxide dismutase activity.. J Ethnopharmacol 2004 Sep;94(1):67-75.
      pubmed: 15261965doi: 10.1016/j.jep.2004.04.023google scholar: lookup
    103. Arent SM, Pellegrino J, McKeever KH. Nutritional supplementation, body composition, performance, and hormonal responses in division I college football players.. Medicine and Science in Sports and Exercise. Supplement 2007;5:p. S361.
    104. Chenal H, Davit-Spraul A, Brevet J. The effects of an orally effective SOD on AIDS West African patients in a randomized double-blinded clinical study.. Proceedings of the XVI International AIDS Conference 2006; Toronto, Canada.
    105. Kick J, Hauser B, Bracht H, Albicini M, Oter S, Simon F, Ehrmann U, Garrel C, Sträter J, Brückner UB, Leverve XM, Schelzig H, Speit G, Radermacher P, Muth CM. Effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping.. Intensive Care Med 2007 Apr;33(4):694-702.
      pubmed: 17237934doi: 10.1007/s00134-006-0518-6google scholar: lookup
    106. McCord JM. Free radicals and inflammation: protection of synovial fluid by superoxide dismutase.. Science 1974 Aug 9;185(4150):529-31.
      pubmed: 4841157doi: 10.1126/science.185.4150.529google scholar: lookup
    107. Betts WH, Cleland LG. Effect of metal chelators and antiinflammatory drugs on the degradation of hyaluronic acid.. Arthritis Rheum 1982 Dec;25(12):1469-76.
      pubmed: 6816249doi: 10.1002/art.1780251213google scholar: lookup
    108. Muth CM, Glenz Y, Klaus M, Radermacher P, Speit G, Leverve X. Influence of an orally effective SOD on hyperbaric oxygen-related cell damage.. Free Radic Res 2004 Sep;38(9):927-32.
      pubmed: 15621710doi: 10.1080/10715760412331273197google scholar: lookup
    109. Baret A, Jadot G, Michelson AM. Pharmacokinetic and anti-inflammatory properties in the rat of superoxide dismutases (Cu SODs and Mn SOD) from various species.. Biochem Pharmacol 1984 Sep 1;33(17):2755-60.
      pubmed: 6466383doi: 10.1016/0006-2952(84)90692-0google scholar: lookup
    110. Arangoa MA, Campanero MA, Renedo MJ, Ponchel G, Irache JM. Gliadin nanoparticles as carriers for the oral administration of lipophilic drugs. Relationships between bioadhesion and pharmacokinetics.. Pharm Res 2001 Nov;18(11):1521-7.
      pubmed: 11758758doi: 10.1023/a:1013018111829google scholar: lookup
    111. Dugas B. Glisodin: a nutraceutical product that promotes the oral delivery of superoxide dismutase.. Free Radical Biology and Medicine 2002;33:p. S64.
    112. Clemente MG, De Virgiliis S, Kang JS, Macatagney R, Musu MP, Di Pierro MR, Drago S, Congia M, Fasano A. Early effects of gliadin on enterocyte intracellular signalling involved in intestinal barrier function.. Gut 2003 Feb;52(2):218-23.
      pmc: PMC1774976pubmed: 12524403doi: 10.1136/gut.52.2.218google scholar: lookup
    113. Drago S, El Asmar R, Di Pierro M, Grazia Clemente M, Tripathi A, Sapone A, Thakar M, Iacono G, Carroccio A, D'Agate C, Not T, Zampini L, Catassi C, Fasano A. Gliadin, zonulin and gut permeability: Effects on celiac and non-celiac intestinal mucosa and intestinal cell lines.. Scand J Gastroenterol 2006 Apr;41(4):408-19.
      pubmed: 16635908doi: 10.1080/00365520500235334google scholar: lookup
    114. Stella V, Vallée P, Albrecht P, Postaire E. Gliadin films. I: preparation and in vitro evaluation as a carrier for controlled drug release.. International Journal of Pharmaceutics 1995;121(1):117–121.
    115. Arangoa MA, Ponchel G, Orecchioni AM, Renedo MJ, Duchêne D, Irache JM. Bioadhesive potential of gliadin nanoparticulate systems.. Eur J Pharm Sci 2000 Oct;11(4):333-41.
      pubmed: 11033077doi: 10.1016/s0928-0987(00)00121-4google scholar: lookup
    116. Personal Communication. Dr. David Griffin. Edgewood, Md, USA: Nutramax Laboratories; 2012.
    117. Powers SK, Ji LL, Leeuwenburgh C. Exercise training-induced alterations in skeletal muscle antioxidant capacity: a brief review.. Med Sci Sports Exerc 1999 Jul;31(7):987-97.
      pubmed: 10416560doi: 10.1097/00005768-199907000-00011google scholar: lookup
    118. Kasapis C, Thompson PD. The effects of physical activity on serum C-reactive protein and inflammatory markers: a systematic review.. J Am Coll Cardiol 2005 May 17;45(10):1563-9.
      pubmed: 15893167doi: 10.1016/j.jacc.2004.12.077google scholar: lookup

    Citations

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      doi: 10.1007/s10439-022-02991-4pubmed: 35754073google scholar: lookup
    2. Ott EC, Cavinder CA, Wang S, Smith T, Lemley CO, Dinh TTN. Oxidative stress biomarkers and free amino acid concentrations in the blood plasma of moderately exercised horses indicate adaptive response to prolonged exercise training. J Anim Sci 2022 Apr 1;100(4).
      doi: 10.1093/jas/skac086pubmed: 35298640google scholar: lookup
    3. Nemec Svete A, Vovk T, Bohar Topolovec M, Kruljc P. Effects of Vitamin E and Coenzyme Q(10) Supplementation on Oxidative Stress Parameters in Untrained Leisure Horses Subjected to Acute Moderate Exercise. Antioxidants (Basel) 2021 Jun 3;10(6).
      doi: 10.3390/antiox10060908pubmed: 34205129google scholar: lookup
    4. Te Moller NCR, Mohammadi A, Plomp S, Serra Bragança FM, Beukers M, Pouran B, Afara IO, Nippolainen E, Mäkelä JTA, Korhonen RK, Töyräs J, Brommer H, van Weeren PR. Structural, compositional, and functional effects of blunt and sharp cartilage damage on the joint: A 9-month equine groove model study. J Orthop Res 2021 Nov;39(11):2363-2375.
      doi: 10.1002/jor.24971pubmed: 33368588google scholar: lookup
    5. Pourmohammad R, Mohri M, Seifi HA, Sardari K. Evaluation of cardiac troponin I, atrial natriuretic peptide and some oxidative/antioxidative biomarkers in the serum and hemolysate of trained Arabian horses after exercise. Iran J Vet Res 2020 Summer;21(3):211-215.
      pubmed: 33178299
    6. Vendruscolo CDP, Moreira JJ, Seidel SRT, Fülber J, Neuenschwander HM, Bonagura G, Agreste FR, Baccarin RYA. Effects of medical ozone upon healthy equine joints: Clinical and laboratorial aspects. PLoS One 2018;13(5):e0197736.
      doi: 10.1371/journal.pone.0197736pubmed: 29813093google scholar: lookup
    7. Kaya O. Effect of a four-week exercise program on the secretion of IFN-γ, TNF-α, IL-2 and IL-6 cytokines in elite Taekwondo athletes. Biomed Rep 2016 Sep;5(3):367-370.
      doi: 10.3892/br.2016.730pubmed: 27588179google scholar: lookup
    8. Duesterdieck-Zellmer KF, Moneta L, Ott JF, Larson MK, Gorman EM, Hunter B, Löhr CV, Payton ME, Morré JT, Maier CS. Effects of low and high dose intraarticular tiludronate on synovial fluid and clinical variables in healthy horses-a preliminary investigation. PeerJ 2014;2:e534.
      doi: 10.7717/peerj.534pubmed: 25237596google scholar: lookup
    9. Wang SZ, He H, Han R, Zhu JL, Kou JQ, Ding XL, Qin ZH. The Protective Effects of Cobra Venom from Naja naja atra on Acute and Chronic Nephropathy. Evid Based Complement Alternat Med 2013;2013:478049.
      doi: 10.1155/2013/478049pubmed: 23983784google scholar: lookup
    10. Carvalho JRG, Sales NAA, Littiere TO, Costa GB, Castro CM, Polisel EEC, Orsi JB, Ramos GV, Santos IFC, Gobatto CA, Manchado-Gobatto FB, Ferraz GC. Acute whole-body vibration as a recovery strategy did not alter the content of gluteus medius monocarboxylate-transporters, lactatemia, and acidosis induced by intense exercise in horses. Front Vet Sci 2025;12:1538195.
      doi: 10.3389/fvets.2025.1538195pubmed: 40115828google scholar: lookup
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