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Home / Equine Research Bank / Int J Mol Sci / Bone Marrow MSC Secretome Increases Equine Articular Chondro...
International journal of molecular sciences2022; 23(10); 5795; doi: 10.3390/ijms23105795

Bone Marrow MSC Secretome Increases Equine Articular Chondrocyte Collagen Accumulation and Their Migratory Capacities.

Abstract: Equine osteoarthritis (OA) leads to cartilage degradation with impaired animal well-being, premature cessation of sport activity, and financial losses. Mesenchymal stem cell (MSC)-based therapies are promising for cartilage repair, but face limitations inherent to the cell itself. Soluble mediators and extracellular vesicles (EVs) secreted by MSCs are the alternatives to overcome those limitations while preserving MSC restorative properties. The effect of equine bone marrow MSC secretome on equine articular chondrocytes (eACs) was analyzed with indirect co-culture and/or MSC-conditioned media (CM). The expression of healthy cartilage/OA and proliferation markers was evaluated in eACs (monolayers or organoids). In vitro repair experiments with MSC-CM were made to evaluate the proliferation and migration of eACs. The presence of nanosized EVs in MSC-CM was appraised with nanoparticle tracking assay and transmission electron microscopy. Our results demonstrated that the MSC secretome influences eAC phenotype by increasing cartilage functionality markers and cell migration in a greater way than MSCs, which could delay OA final outcomes. This study makes acellular therapy an appealing strategy to improve equine OA treatments. However, the MSC secretome contains a wide variety of soluble mediators and small EVs, such as exosomes, and further investigation must be performed to understand the mechanisms occurring behind these promising effects.
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Publication Date: 2022-05-21 PubMed ID: 35628604PubMed Central: PMC9146805DOI: 10.3390/ijms23105795Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the effects of certain equine bone marrow stem cell (MSC) secretions on the chondrocyte cells in horse’s joint cartilage, and suggests potential benefits in treating equine osteoarthritis (OA).

Objective of the Research

  • The primary objective of the research is to evaluate the effect of the secretome (the total of the substances secreted by a cell) from equine bone marrow MSCs on the chondrocyte cells in horse cartilage. These chondrocyte cells are responsible for maintaining the functionality and integrity of cartilage, the degradation of which is a primary factor in equine OA.

Methodology

  • The research used indirect co-culture and cell conditioned media to analyze the effects of the MSC secretome on equine articular chondrocytes (eACs).
  • Various markers of healthy cartilage, osteoarthritis, and cell proliferation were evaluated in eACs, which were grown both as single-layer cells and in organoid (3D cell cluster) form.
  • In vitro (outside the living body) experiments were conducted to assess the effect of MSC conditioned media on the proliferation and migration (movement) of eACs.
  • Techniques like nanoparticle tracking assay and transmission electron microscopy were employed to verify the presence of nano-sized extracellular vesicles in the MSC conditioned media.

Findings

  • The study found that the MSC secretome had a significant impact on the phenotype of eACs by increasing the markers of cartilage functionality and enhancing cell migration. This suggests that the MSC secretome may be more effective in improving cartilage health than MSCs alone.
  • Consequently, the use of the MSC secretome could potentially delay the progression of OA.

Implications and Future Research

  • The research findings make the use of the secretome from MSCs a promising avenue for developing non-cellular, or ‘acellular’, therapies to improve treatments for equine OA. Harnessing the beneficial effects of cellular secretion, rather than the cells themselves, could help overcome some of the current limitations of stem cell therapies.
  • However, the MSC secretome contains a variety of substances, including soluble mediators and small extracellular vesicles like exosomes. Therefore, further research is needed to fully understand the underlying mechanisms that generate the observed beneficial effects.

Cite This Article

APA
Contentin R, Jammes M, Bourdon B, Cassé F, Bianchi A, Audigié F, Branly T, Velot É, Galéra P. (2022). Bone Marrow MSC Secretome Increases Equine Articular Chondrocyte Collagen Accumulation and Their Migratory Capacities. Int J Mol Sci, 23(10), 5795. https://doi.org/10.3390/ijms23105795

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 23
Issue: 10
PII: 5795

Researcher Affiliations

Contentin, Romain
  • Normandie University, Unicaen, Biotargen, F-14000 Caen, France.
Jammes, Manon
  • Normandie University, Unicaen, Biotargen, F-14000 Caen, France.
Bourdon, Bastien
  • Normandie University, Unicaen, Biotargen, F-14000 Caen, France.
Cassé, Frédéric
  • Normandie University, Unicaen, Biotargen, F-14000 Caen, France.
Bianchi, Arnaud
  • Molecular Engineering and Articular Physiopathology (IMoPA), French National Center for Scientific Research (CNRS), Université de Lorraine, F-54000 Nancy, France.
Audigié, Fabrice
  • Center of Imaging and Research on Locomotor Affections on Equines (CIRALE), Unit Under Contract 957 Equine Biomechanics and Locomotor Disorders (USC 957 BPLC), French National Research Institute for Agriculture Food and Environment (INRAE), École Nationale Vétérinaire d'Alfort, F-94700 Maisons-Alfort, France.
Branly, Thomas
  • Normandie University, Unicaen, Biotargen, F-14000 Caen, France.
Velot, Émilie
  • Molecular Engineering and Articular Physiopathology (IMoPA), French National Center for Scientific Research (CNRS), Université de Lorraine, F-54000 Nancy, France.
Galéra, Philippe
  • Normandie University, Unicaen, Biotargen, F-14000 Caen, France.

MeSH Terms

  • Animals
  • Bone Marrow / metabolism
  • Chondrocytes / metabolism
  • Collagen / metabolism
  • Culture Media, Conditioned / metabolism
  • Culture Media, Conditioned / pharmacology
  • Horses
  • Mesenchymal Stem Cells / metabolism
  • Osteoarthritis / metabolism
  • Osteoarthritis / therapy
  • Secretome

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

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