Changes in blood gas, acid-base and metabolic parameters in horses during three-day event competition.

This research investigates changes in blood gas, acid-base, and metabolic parameters in horses while engaged in a three-day competitive event. The study analyzes blood samples to examine changes in these parameters across different stages of the competition.

Examined Parameters

• The research focuses on several critical parameters in venous blood samples from the competing horses. These parameters include:
  • Blood gas
  • Acid-base balance
  • Concentrations of several metabolites: lactate, pyruvate, beta-hydroxybutyrate, acetoacetate, and alpha-ketoglutarate
  • Cortisol levels, which provide insights into the stressful impact of the competition on the horses

Research Methodology and Phases

• The study was conducted during a three-day event and involved different stages of exertion. Blood samples were collected during the speed and endurance test, which took place on day two.
• The study tracked changes across distinct phases of the competition: the roads and tracks phase, the steeplechase phase, and the cross country section.

Results and Findings

• During the roads and tracks and steeplechase phases, the study found significant metabolic and respiratory alkalosis in the horses, despite an increase in lactate levels. Alkalosis indicates a shift in the body’s pH above its normal range, suggesting possible systemic changes in the horses from the exertion.
• At the conclusion of the cross country section, there was a significant drop in total base, a blood parameter influencing pH. However, the horses’ pH did not significantly change from pre-event levels despite this decrease in total base.
• The consistent pH levels despite the drop in base were attributed to a simultaneous fall in carbon dioxide levels. Directly related to respiration, a fall in carbon dioxide can balance the pH change created by a decrease in the total base, explaining the steady pH values.