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Home / Equine Research Bank / Animals (Basel) / Changes in Calprotectin (S100A8-A9) and Aldolase in the Sali...
Animals : an open access journal from MDPI2023; 13(8); doi: 10.3390/ani13081367

Changes in Calprotectin (S100A8-A9) and Aldolase in the Saliva of Horses with Equine Gastric Ulcer Syndrome.

Abstract: Equine gastric ulcer syndrome (EGUS) is a highly prevalent disease that affects horses worldwide. Within EGUS, two different forms have been described: equine squamous gastric disease (ESGD) and equine glandular gastric disease (EGGD). The associated clinical signs cause detrimental activity performance, reducing the quality of life of animals. Saliva can contain biomarkers for EGUS that could be potentially used as a complementary tool for diagnosis. The objective of this work was to evaluate the measurements of calprotectin (CALP) and aldolase in the saliva of horses as potential biomarkers of EGUS. For this purpose, automated assays for the quantification of these two proteins were analytically validated and applied for detecting EGUS in a total of 131 horses divided into 5 groups: healthy horses, ESGD, EGGD, combined ESGD and EGGD, and horses with other intestinal pathologies. The assays showed good precision and accuracy in analytical validation, and they were able to discriminate between horses with EGUS and healthy horses, especially in the case of CALP, although they did not show significant differences between horses with EGUS and horses with other diseases. In conclusion, salivary CALP and aldolase can be determined in the saliva of horses and further studies are warranted to elucidate the potential of these analytes as biomarkers in EGUS.
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Publication Date: 2023-04-16 PubMed ID: 37106929PubMed Central: PMC10135270DOI: 10.3390/ani13081367Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper explores the possibility of using salivary levels of two proteins, calprotectin (CALP) and aldolase, as indicative markers for equine gastric ulcer syndrome (EGUS), a common disease in horses. The study found that measuring these biomarkers could help to distinguish between healthy horses and those affected by EGUS, particularly using CALP, but further research is required to confirm their potential role in diagnosing this condition.

Understanding the Research

  • The study focuses on Equine Gastric Ulcer Syndrome (EGUS), a widespread disease affecting horses globally. The illness comes in two forms, equine squamous gastric disease (ESGD) and equine glandular gastric disease (EGGD). Its symptoms can severely impact an animal’s performance and degrade its life quality.
  • The researchers hypothesized that the saliva of horses could hold biomarkers for EGUS that might aid or supplement traditional diagnostic methods. They decided to evaluate two specific proteins found in saliva, known as calprotectin (CALP) and aldolase, for their potential as EGUS biomarkers.

Methodology and Results

  • Automated assays were used to quantify the levels of CALP and aldolase in the saliva of a total of 131 horses. These animals were categorized into five groups: healthy horses, those with ESGD, those with EGGD, those with both ESGD and EGGD, and horses with other intestinal diseases.
  • The assays demonstrated good accuracy and precision in their analytical validation process. They managed to distinguish between healthy horses and those with EGUS, with calprotectin proving particularly useful in making this distinction.
  • However, the assays didn’t show significant differences between horses with EGUS and those with other types of intestinal diseases. This suggests more specific or sensitive biomarkers might be needed to accurately diagnose EGUS.

Conclusions

  • The study concludes that it is possible to measure the salivary levels of CALP and aldolase in horses, and these proteins might serve as potential EGUS biomarkers.
  • However, further studies are needed to fully understand the potential of these two proteins as diagnostic tools for EGUS. Particularly, more research is required to differentiate between EGUS and other digestive system diseases in horses.

Cite This Article

APA
Muñoz-Prieto A, Contreras-Aguilar MD, Cerón JJ, Ayala de la Peña I, Martín-Cuervo M, Eckersall PD, Holm Henriksen IM, Tecles F, Hansen S. (2023). Changes in Calprotectin (S100A8-A9) and Aldolase in the Saliva of Horses with Equine Gastric Ulcer Syndrome. Animals (Basel), 13(8). https://doi.org/10.3390/ani13081367

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 8

Researcher Affiliations

Muñoz-Prieto, Alberto
  • Interdisciplinary Laboratory of Clinical Analysis (INTERLAB-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, 30100 Murcia, Spain.
Contreras-Aguilar, María Dolores
  • Interdisciplinary Laboratory of Clinical Analysis (INTERLAB-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, 30100 Murcia, Spain.
Cerón, José Joaquín
  • Interdisciplinary Laboratory of Clinical Analysis (INTERLAB-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, 30100 Murcia, Spain.
Ayala de la Peña, Ignacio
  • Interdisciplinary Laboratory of Clinical Analysis (INTERLAB-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, 30100 Murcia, Spain.
Martín-Cuervo, María
  • Animal Medicine, Faculty of Veterinary Medicine of Cáceres, University of Extremadura, Av. de la Universidad S-N, 10005 Cáceres, Spain.
Eckersall, Peter David
  • Interdisciplinary Laboratory of Clinical Analysis (INTERLAB-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, 30100 Murcia, Spain.
  • School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Bearsden Rd, Glasgow G61 1QH, UK.
Holm Henriksen, Ida-Marie
  • Department of Veterinary Clinical Sciences, Veterinary School of Medicine, Sektion Medicine and Surgery, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Høje-Taastrup, Denmark.
Tecles, Fernando
  • Interdisciplinary Laboratory of Clinical Analysis (INTERLAB-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, 30100 Murcia, Spain.
Hansen, Sanni
  • Animal Medicine, Faculty of Veterinary Medicine of Cáceres, University of Extremadura, Av. de la Universidad S-N, 10005 Cáceres, Spain.

Grant Funding

  • 19894/GERM/15 / Fundación Séneca - Agencia de Ciencia y Tecnología de la Región de Murcia
  • RYC2021-033660-I / Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 6 times.
  1. Bishop RC, Arrington JV, Wilkins PA, McCoy AM. Alterations in the Peritoneal Fluid Proteome of Horses with Colic Attributed to Ischemic and Non-Ischemic Intestinal Disease. Animals (Basel) 2025 May 30;15(11).
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