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Computational and structural biotechnology journal2022; 21; 418-424; doi: 10.1016/j.csbj.2022.12.016

Changes in novel anti-infalmmatory cytokine concetration in the bood of endurance and race horses at different levels of training.

Abstract: Several anti-inflammatory cytokines have been proposed as markers for exercise monitoring in humans such as the interleukin 1 receptor agonist (IL-ra), or interleukin 13 (IL-13). Equine athletes may be considered a model for human exercise physiology research, however there is a lack of such studies of this species. Thus, we decided to examine the changes of IL-1ra and IL-13 in serum concentration during aerobic (endurance) and anaerobic (race) exercise in horses of different fitness levels in comparison with the well-known anti-inflammatory cytokine interleukin 10 (IL-10). The group of endurance horses (n = 13) consisted of animals competing over 100 (n = 7) and 120 km (n = 6) rides. The group of racehorses (n = 18) consisted of trained (n = 9) and untrained (n = 9) animals. The blood samples were obtained before and after the exercise. The ELISA test was performed to evaluate the changes of IL-1ra, IL-13 and IL-10 during different types of exercise. In endurance horses there was an increase in IL-13 (p = 0.0012) after the 100 km ride and in IL-1ra (p = 0.0411) after the 120 km ride. In race horses there was a higher IL-13 basal serum concentration in the untrained group, as well as a decrease of IL-13 after exercise (p = 0.0188). In trained racehorses there was an increase in IL-1ra (p < 0.0001) and IL-13 after exercise (p = 0.0028). In conclusion, the reaction of IL-1ra and IL-13 to different types of exercise differ from each other. Thus, in future, they may be helpful in monitoring the fitness of horses, however more research is needed.
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Publication Date: 2022-12-13 PubMed ID: 36618977PubMed Central: PMC9798135DOI: 10.1016/j.csbj.2022.12.016Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper focuses on understanding the changes in anti-inflammatory cytokines in horses participating in aerobic and anaerobic activities. It specifically studies how Interleukin 1 receptor agonist (IL-1ra) and interleukin 13 (IL-13) concentrations in the blood vary in endurance and race horses of different fitness levels.

Research Methodology

  • The study divided horses into two main groups: endurance horses, and racing horses. The endurance group contained 13 horses, while the racing group had 18 horses.
  • The endurance horses competed over 100km (7 horses) and 120km (6 horses) rides. The racing horses were further split into trained (9 horses) and untrained (9 horses) animals.
  • Blood samples were collected from these horses both before and after each exercise.
  • The research used the ELISA test, a popular technique for measuring antibodies in the blood.

Research Findings

  • Results showed an increase in IL-13 in endurance horses after the 100km ride, and an increase in IL-1ra after the 120km ride.
  • In racehorses, untrained horses showed higher baseline IL-13 serum concentrations, and decreased IL-13 levels after exercise.
  • Trained racehorses, on the other hand, showed an increase in both IL-1ra and IL-13 levels after exercise.

Conclusion

  • Results concluded that the response of IL-1ra and IL-13 to different types of exercises in horses differed from each other. Such findings may aid in monitoring horses’ fitness in the future.
  • However, the researchers acknowledged the need for more research in this area to further understand the importance and implications of these cytokines in equine fitness and exercise physiology.

Cite This Article

APA
Plisak U, Szczepaniak J, Żmigrodzka M, Giercuszkiewicz-Hecold B, Witkowska-Piłaszewicz O. (2022). Changes in novel anti-infalmmatory cytokine concetration in the bood of endurance and race horses at different levels of training. Comput Struct Biotechnol J, 21, 418-424. https://doi.org/10.1016/j.csbj.2022.12.016

Publication

Computational and structural biotechnology journal
ISSN: 2001-0370
NlmUniqueID: 101585369
Country: Netherlands
Language: English
Volume: 21
Pages: 418-424

Researcher Affiliations

Plisak, Urszula
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
  • Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
Szczepaniak, Jarosław
  • Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
Żmigrodzka, Magdalena
  • Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
Giercuszkiewicz-Hecold, Beata
  • Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
Witkowska-Piłaszewicz, Olga
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
  • Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 9 times.
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