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Home / Equine Research Bank / Animals (Basel) / Changes in Oxidative Status Biomarkers in Saliva and Serum i...
Animals : an open access journal from MDPI2022; 12(5); doi: 10.3390/ani12050667

Changes in Oxidative Status Biomarkers in Saliva and Serum in the Equine Gastric Ulcer Syndrome and Colic of Intestinal Aetiology: A Pilot Study.

Abstract: Changes in the oxidative status of the blood of horses suffering from gastric ulcers and colic of intestinal aetiology (CIE) have been reported. However, saliva can also be a source of biomarkers of oxidative status. Therefore, this study aims to validate automated assays for the measurement of oxidative status biomarkers (ferric reducing ability of saliva/serum-FRAS/FRAP, cupric reducing antioxidant capacity-CUPRAC, the Trolox equivalent antioxidant capacity-TEAC, uric acid, and advanced oxidation protein products-AOPP) in the saliva and serum of horses, to assess their changes in the different ulcer gastric diseases (squamous-ESGD and glandular-EGGD) and CIE, and to evaluate their relationship with serum amyloid A (SAA), adenosine deaminase (ADA), and the systemic inflammatory response syndrome (SIRS) status. The assays showed a low imprecision and good linearity with enough sensitivity in both fluids. In EGGD, higher levels of FRAS, uric acid, and AOPP in saliva were observed compared to the healthy group, correlating with the salivary ADA levels. Horses with CIE showed increases in uric acid concentrations in serum associated with their SIRS status and outcome of the disease. In conclusion, analytes related to the oxidative status can be measured in the saliva and serum from horses by automated assays, and some of them can potentially be assessed as biomarkers in horses with gastric ulcers and CIE.
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Publication Date: 2022-03-07 PubMed ID: 35268236PubMed Central: PMC8909870DOI: 10.3390/ani12050667Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article discusses a study that was conducted to validate assays for measuring oxidative status biomarkers in the saliva and serum of horses suffering from gastric ulcers and intestinal colic. The study showed that these biomarkers can potentially help in assessing these conditions in horses.

Objective of the Study

  • The primary goal of this research was to validate the effectiveness of automated assays in measuring biomarkers of oxidative status in the saliva and serum in horses. This was evaluated on horses suffering from gastric ulcers and colic of intestinal aetiology with the intention of using these biomarkers as a possible means of assessing such medical conditions.

Methodology

  • The researchers used established tests for measuring oxidative status biomarkers, including FRAS (ferric reducing ability of saliva/serum), FRAP (Ferric Reducing Ability of Plasma), CUPRAC (cupric reducing antioxidant capacity), TEAC (Trolox equivalent antioxidant capacity), uric acid, and AOPP (advanced oxidation protein products).
  • These measurements were conducted on horses with different types of gastric ulcers and colic of intestinal aetiology, comparing results against a healthy group of horses.

Results

  • The study found that these assays presented low imprecision and good linearity with sufficient sensitivity levels to be potentially useful for veterinary medical purposes.
  • In horses with glandular gastric diseases (EGGD), there were higher levels of FRAS, uric acid, and AOPP in their saliva as compared to the healthy group. These findings were correlated with the salivary ADA (adenosine deaminase) levels.
  • In horses afflicted with colic of intestinal aetiology, the researchers found increases in serum uric acid concentrations, correlated with their SIRS (systemic inflammatory response syndrome) status and the disease outcome.

Conclusion

  • The results indicated that oxidative status, as measured by the above-mentioned assays, could be effectively determined in saliva and serum samples from horses.
  • Some of the biomarkers measured by these assays could potentially be useful diagnostic tools for horses with gastric ulcers and intestinal colic.

Cite This Article

APA
Contreras-Aguilar MD, Rubio CP, González-Arostegui LG, Martín-Cuervo M, Cerón JJ, Ayala I, Henriksen IH, Jacobsen S, Hansen S. (2022). Changes in Oxidative Status Biomarkers in Saliva and Serum in the Equine Gastric Ulcer Syndrome and Colic of Intestinal Aetiology: A Pilot Study. Animals (Basel), 12(5). https://doi.org/10.3390/ani12050667

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 5

Researcher Affiliations

Contreras-Aguilar, María Dolores
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Rubio, Camila Peres
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
  • Department of Animal and Food Science, School of Veterinary Science, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain.
González-Arostegui, Luis Guillermo
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Martín-Cuervo, María
  • Medicine Animal, Faculty of Veterinary Medicine of Cáceres, University of Extremadura, Avenida de la Universidad S-N, 10002 Cáceres, Spain.
Cerón, Jose J
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Ayala, Ignacio
  • Department of Animal Medicine & Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Henriksen, Ida-Marie Holm
  • Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, DK-2630 Taastrup, Denmark.
Jacobsen, Stine
  • Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, DK-2630 Taastrup, Denmark.
Hansen, Sanni
  • Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, DK-2630 Taastrup, Denmark.

Grant Funding

  • 19894/GERM/15 / Fundación Seneca
  • FJC2019-042475-I / Juan de la Cierva Formación, Ministerio de Economía y Competitividad

Conflict of Interest Statement

The authors declare no conflict of interest.

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