Changes in skeletal muscle GLUT4 content and muscle membrane glucose transport following 6 weeks of exercise training.
Abstract: This study examined changes in skeletal muscle GLUT4 content and glucose transport in isolated muscle membranes (GT) from horses before and 2 min after standardised submaximal exercise tests (SET) prior to and after completion of 6 weeks of training. Seven horses, age 3-9 years, body mass mean +/- s.e. 530 +/- 19 kg, and sedentary for at least 4 months, completed 6 weeks of training on a treadmill. An initial SET (UT) was performed on a 4 degree incline at a speed equivalent to 55% of pretraining VO2max and was repeated post-training at the same absolute workload (ABS). A third SET (REL) was performed at 55% of post-training VO2max. There was no significant pre- to postexercise change in GLUT4 content before or after training. Following training, total GLUT4 content was increased 2- or 3-fold in pre-exercise biopsies (pre UT: 0.30 +/- 0.05; pre ABS: 1.05 +/- 0.32; pre REL: 1.34 +/- 0.28 arbitrary units) (P<0.05) with similar increases in postexercise GLUT4 content (P<0.05) (post UT: 033 +/- 0.06; post ABS: 1.19 +/- 0.44; post REL: 1.43 +/- 0.31). GT increased 2.5- to 6-fold in postexercise muscle membrane vesicles in UT over a range of glucose concentrations. After training (ABS and REL), there was a 25-50% attenuation (P<0.05) in membrane GT in response to exercise in ABS and REL. These finding indicate that moderate intensity exercise training increased middle gluteal muscle GLUT4 content, but this change was not reflected in an increase in muscle membrane glucose transport activity in postexercise muscle samples.
Publication Date: 2002-10-31 PubMed ID: 12405686DOI: 10.1111/j.2042-3306.2002.tb05418.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research explored how six weeks of exercise training affected the levels of GLUT4, a glucose transporter, in skeletal muscles of horses. In particular, it examined GLUT4 content and the glucose transport activity of muscle membranes before and after training.
Study Design
- Seven horses aged between 3-9 years, and inactive for at least four months, were selected. The body mass averaged 530 kg.
- These horses underwent six weeks of training on a treadmill.
- A standardized submaximal exercise test (SET) was performed on a 4-degree incline at a speed equivalent to 55% of the horse’s pre-training maximum oxygen uptake (VO2max).
- This SET was repeated after the training, once at the same absolute workload (ABS), and another relative to the post-training VO2max (REL).
- Changes in the GLUT4 content and the glucose transport (GT) activity of isolated muscle membranes were assessed before and two minutes after the exercise tests.
Results
- When it comes to GLUT4 content, no significant changes were observed immediately following the exercise test before or after training.
- However, after the training, total GLUT4 content in pre-exercise biopsies had increased 2- or 3-fold. There were similar increases in post-exercise GLUT4 content, suggesting that the training affected GLUT4 levels.
- The GT increased 2.5- to 6-fold in post-exercise muscle membrane vesicles in the untrained state over a range of glucose concentrations.
- After training, there was a 25-50% reduction in membrane GT in response to exercise in ABS and REL.
Conclusions
- The findings demonstrate that moderate intensity exercise training increased GLUT4 content in middle gluteal muscle.
- Despite the increase in GLUT4, this change didn’t result in an increase in muscle membrane glucose transport activity in the post-exercise muscle samples. This suggests that while training can increase GLUT4 levels, it does not necessarily elevate the ability of muscle membranes to transport glucose as might be expected.
Cite This Article
APA
McCutcheon LJ, Geor RJ, Hinchcliff KW.
(2002).
Changes in skeletal muscle GLUT4 content and muscle membrane glucose transport following 6 weeks of exercise training.
Equine Vet J Suppl(34), 199-204.
https://doi.org/10.1111/j.2042-3306.2002.tb05418.x Publication
Researcher Affiliations
- Department of Pathobiology, Ontario Veterinary College, University of Guelph, Canada.
MeSH Terms
- Analysis of Variance
- Animals
- Biological Transport / physiology
- Biopsy / veterinary
- Exercise Test / veterinary
- Glucose / metabolism
- Glucose Transporter Type 4
- Horses / metabolism
- Horses / physiology
- Longitudinal Studies
- Male
- Monosaccharide Transport Proteins / analysis
- Monosaccharide Transport Proteins / metabolism
- Muscle Proteins
- Muscle, Skeletal / chemistry
- Muscle, Skeletal / metabolism
- Oxygen Consumption
- Physical Conditioning, Animal / physiology
- Pulmonary Gas Exchange
- Time Factors
Citations
This article has been cited 8 times.- Vidal Moreno de Vega C, Lemmens D, de Meeûs d'Argenteuil C, Boshuizen B, de Maré L, Leybaert L, Goethals K, de Oliveira JE, Hosotani G, Deforce D, Van Nieuwerburgh F, Devisscher L, Delesalle C. Dynamics of training and acute exercise-induced shifts in muscular glucose transporter (GLUT) 4, 8, and 12 expression in locomotion versus posture muscles in healthy horses. Front Physiol 2023;14:1256217.
- Torres-Aguilera I, Pinto-Hernandez P, Iglesias-Gutierrez E, Terrados N, Fernandez-Sanjurjo M. Circulating plasma levels of miR-106b-5p predicts maximal performance in female and male elite kayakers. Front Sports Act Living 2023;5:1040955.
- Box JR, McGowan CM, Raekallio MR, Mykkänen AK, Carslake H, Karikoski NP. Insulin dysregulation in a population of Finnhorses and associated phenotypic markers of obesity. J Vet Intern Med 2020 Jul;34(4):1599-1605.
- Dunkel B, Knowles EJ, Chang YM, Menzies-Gow NJ. Correlation between l-lactate and glucose concentrations and body condition score in healthy horses and ponies. J Vet Intern Med 2019 Sep;33(5):2267-2271.
- Durham AE, Frank N, McGowan CM, Menzies-Gow NJ, Roelfsema E, Vervuert I, Feige K, Fey K. ECEIM consensus statement on equine metabolic syndrome. J Vet Intern Med 2019 Mar;33(2):335-349.
- Davis MS, Bonen A, Snook LA, Jain SS, Bartels K, Geor R, Hr K. Conditioning increases the gain of contraction-induced sarcolemmal substrate transport in ultra-endurance racing sled dogs. PLoS One 2014;9(7):e103087.
- Boshuizen B, De Maré L, Oosterlinck M, Van Immerseel F, Eeckhaut V, De Meeus C, Devisscher L, Vidal Moreno de Vega C, Willems M, De Oliveira JE, Hosotani G, Gansemans Y, Meese T, Van Nieuwerburgh F, Deforce D, Vanderperren K, Verdegaal EL, Delesalle C. Aleurone supplementation enhances the metabolic benefits of training in Standardbred mares: impacts on glucose-insulin dynamics and gut microbiome composition. Front Physiol 2025;16:1565005.
- Valberg SJ, Velez-Irizarry D, Williams ZJ, Pagan JD, Mesquita V, Waldridge B, Maresca-Fichter H. Novel Expression of GLUT3, GLUT6 and GLUT10 in Equine Gluteal Muscle Following Glycogen-Depleting Exercise: Impact of Dietary Starch and Fat. Metabolites 2023 Jun 1;13(6).
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