Characteristics and epidemiological investigation of equid herpesvirus 8 in donkeys in Shandong, China.
Abstract: Equid herpesvirus 8 (EHV-8), also known as asinine herpesvirus type 3 (AHV-3), can cause severe respiratory disease, abortion in mares, and neurological disorders. There is limited information on the prevalence of EHV-8 in donkeys in China. In this study, we investigated EHV-8 infection in donkeys using PCR, resulting in the identification of a field strain, termed EHV-8 SD2020113, which was isolated using RK-13 cells and characterized by high-throughput sequencing and transmission electron microscopy. Our data indicated that 38.7% (457/1180) of donkeys showed the presence of EHV-8 in blood samples. Analysis of the ORF70 gene showed the highest similarity (99.8-99.9% identity) to EHV-8 IR/2015/40 (MF431614.1) and SDLC66 (MW816102), and, in phylogenetic analysis, it clustered with EHV-8 SDLC66 from China. The findings of this study indicate that EHV-8 is likely to represent a threat to the donkey industry, and breeders and veterinarians who care for donkey farms should be aware of this.
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
Publication Date: 2023-03-04 PubMed ID: 36871102PubMed Central: 5129218DOI: 10.1007/s00705-023-05704-xGoogle Scholar: Lookup
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Summary
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This research investigates the prevalence and characteristics of Equid herpesvirus 8 (EHV-8), a disease that can cause serious health problems in donkeys, in Shandong, China. The researchers found that almost 39% of sampled donkeys had the virus.
Objective and Methodology
- The primary object of this research was to study the prevalence, characteristics, and epidemiological aspects of Equid Herpesvirus 8 (EHV-8), also known as Asinine Herpesvirus type 3 (AHV-3), in donkeys in Shandong, China. This virus is known to cause severe respiratory disease, abortions in mares, and neurological disorders.
- The researchers used PCR (Polymerase Chain Reaction), a technique to amplify microscopic amounts of DNA, to detect the presence of EHV-8.
- A strain was detected, termed EHV-8 SD2020113, and was isolated for further study using RK-13 cells. The characteristics of this strain were analyzed using high-throughput sequencing methods and transmission electron microscopy.
Results and Findings
- The data indicates that a significant proportion, about 38.7% (457/1180), of the donkeys carry EHV-8 in their blood samples, which suggests that the prevalence of the virus is high.
- Further analysis of the structure of the virus, focusing on ORF70 gene, demonstrated a high degree of similarity (99.8-99.9% identity) to previously known strains EHV-8 IR/2015/40 (MF431614.1) and SDLC66 (MW816102).
- The phylogenetic analysis, which provides a snapshot of the relationship between different species based on their genetic traits, showed that the new strain clustered closely with EHV-8 SDLC66, a strain previously identified from China.
Implications
- The high prevalence of EHV-8 amongst the sampled donkeys indicates that this virus is likely to be a significant threat to the donkey industry.
- There is a need for breeders and veterinarians who interact with donkey farms to be aware of this finding and take preventative measures to control the spread of this virus.
Cite This Article
APA
Wang T, Xi C, Yu Y, Liu W, Akhtar MF, Li Y, Wang C, Li L.
(2023).
Characteristics and epidemiological investigation of equid herpesvirus 8 in donkeys in Shandong, China.
Arch Virol, 168(3), 99.
https://doi.org/10.1007/s00705-023-05704-x Publication
Researcher Affiliations
- College of Agronomy, Liaocheng University, Liaocheng, China.
- College of Agronomy, Liaocheng University, Liaocheng, China.
- College of Agronomy, Liaocheng University, Liaocheng, China.
- College of Agronomy, Liaocheng University, Liaocheng, China.
- College of Agronomy, Liaocheng University, Liaocheng, China.
- College of Agronomy, Liaocheng University, Liaocheng, China. liyubao@lcu.edu.cn.
- College of Agronomy, Liaocheng University, Liaocheng, China. wangchangfa@lcu.edu.cn.
- College of Agronomy, Liaocheng University, Liaocheng, China. lifeiyang2017@163.com.
MeSH Terms
- Pregnancy
- Horses
- Animals
- Female
- Equidae
- Phylogeny
- China
- Farms
- High-Throughput Nucleotide Sequencing
- Varicellovirus
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