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Home / Equine Research Bank / Vet Microbiol / Characterization of an equine macrophage cell line: applicat...
Veterinary microbiology2008; 136(1-2); 8-19; doi: 10.1016/j.vetmic.2008.10.010

Characterization of an equine macrophage cell line: application to studies of EIAV infection.

Abstract: EIAV is a monocyte/macrophage tropic virus. To date, even though EIAV has been under investigation for numerous years, very few details have been elucidated about EIAV/macrophage interactions. This is largely due to the absence of an equine macrophage cell line that would support viral replication. Herein we describe the spontaneous immortalization and generation of a clonal equine macrophage-like (EML) cell line with the functional and immunophenotype characteristics of differentiated equine monocyte derived macrophage(s) (eMDM(s)). These cells possess strong non-specific esterase (NSE) activity, are able to phagocytose fluorescent bioparticles, and produce nitrites in response to LPS. The EML-3C cell line expresses the EIAV receptor for cellular entry (ELR1) and supports replication of the virulent EIAV(PV) biological clone. Thus, EML-3C cells provide a useful cell line possessing equine macrophage related properties for the growth and study of EIAV infection as well as of other equine macrophage tropic viruses.
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Publication Date: 2008-11-01 PubMed ID: 19038510PubMed Central: PMC2689946DOI: 10.1016/j.vetmic.2008.10.010Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper talks about characterizing an equine macrophage cell line that can help in the study of how the Equine Infectious Anemia Virus (EIAV) infects its host. This was done in order to understand the EIAV infection better and further research on potential cures or preventative measures.

Background

  • EIAV or Equine Infectious Anemia Virus is a virus that affects horses and similar mammals, and is known to be attracted to and infect monocyte/macrophage cells.
  • Despite many years of study, researchers still don’t know a lot about how EIAV interacts with macrophages. This lack of data is primarily because there has been no stable equine macrophage cell line that supports viral replication, which is essential for the study of the virus’s lifecycle and interactions.

Research Procedures

  • In this study, the researchers describe the creation of an equine macrophage-like cell line (EML). This was achieved through spontaneous immortalization and establishing a clonal line.
  • The new cell line was then studied to validate its likeness in function and immunophenotype characteristics with natural equine monocyte-derived macrophage(s) (eMDM(s)). The validation showed the cells have strong non-specific esterase (NSE) activity, and they are capable of phagocytosis and nitrite production in response to lipopolysaccharides (LPS).

Results and Conclusion

  • The research proves that the resultant EML-3C cell line effectively expresses the EIAV receptor used for cellular entry (ELR1). The cell line also supports the replication of the EIAV(PV) biological clone, a common stark strain of the virus.
  • These characteristics make the EML-3C cells a highly useful cell line for further understanding and studying EIAV infections, as well as other viruses that also primarily affect equine macrophages.

Cite This Article

APA
Fidalgo-Carvalho I, Craigo JK, Barnes S, Costa-Ramos C, Montelaro RC. (2008). Characterization of an equine macrophage cell line: application to studies of EIAV infection. Vet Microbiol, 136(1-2), 8-19. https://doi.org/10.1016/j.vetmic.2008.10.010

Publication

Veterinary microbiology
ISSN: 0378-1135
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 136
Issue: 1-2
Pages: 8-19

Researcher Affiliations

Fidalgo-Carvalho, Isabel
  • Iron Genes and the Immune System, Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, Oporto, Portugal.
Craigo, Jodi K
    Barnes, Shannon
      Costa-Ramos, Carolina
        Montelaro, Ronald C

          MeSH Terms

          • Animals
          • Carboxylesterase / blood
          • Cell Line
          • Equine Infectious Anemia / blood
          • Equine Infectious Anemia / virology
          • Flow Cytometry / veterinary
          • Horses / blood
          • Immunophenotyping / veterinary
          • Infectious Anemia Virus, Equine / physiology
          • Macrophages / cytology
          • Macrophages / immunology
          • Macrophages / virology
          • Male
          • Mice
          • Microscopy, Fluorescence / veterinary
          • Microscopy, Phase-Contrast / veterinary
          • NIH 3T3 Cells
          • Nitrites / analysis
          • Nitrites / blood
          • Phagocytosis
          • Virus Replication

          Grant Funding

          • R01 AI025850 / NIAID NIH HHS
          • R01 AI025850-20 / NIAID NIH HHS
          • R56AI07326 / NIAID NIH HHS
          • R01AI02580 / NIAID NIH HHS

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          Citations

          This article has been cited 4 times.
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