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American journal of veterinary research2015; 76(4); 358-366; doi: 10.2460/ajvr.76.4.358

Characterization of discrete equine intestinal epithelial cell lineages.

Abstract: To characterize epithelial cells of the small intestine and colon in horses without clinical gastrointestinal abnormalities with an emphasis on the stem cell niche constituents. Methods: Mucosal biopsy specimens from small and large intestines obtained from 12 horses euthanized for reasons unrelated to gastrointestinal disease or systemic disease. Methods: Intestinal biopsy specimens were collected by sharp dissection immediately following euthanasia. Specimens were prepared for immunohistochemical, immunofluorescence, and transmission electron microscopic imaging to detect and characterize each epithelial cell type. Antibodies against protein biomarkers for cellular identification were selected on the basis of expression in other mammalian species. Results: Intestinal epithelial cell types were identified by means of immunostaining and morphological characterization with transmission electron microscopy. Some differences in biomarker expression and antibody cross-reactivity were identified in equine tissue, compared with other species. However, each known type of mucosal epithelial cell was identified in equine tissue. Conclusions: The methodology used can enhance detection of stem cells and progenitor cells as well as postmitotic cell lineages in equine intestinal tissues. Results may have relevance to regenerative potential of intestinal mucosa and survival in horses with colic.
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Publication Date: 2015-03-31 PubMed ID: 25815577PubMed Central: PMC4517574DOI: 10.2460/ajvr.76.4.358Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the types of epithelial cells in the small intestine and colon of healthy horses, especially focusing on the composition of stem cells. The researchers used a variety of microscopy and antibody staining techniques for accurate identification.

Methods of the Research

  • The research focused on the investigation of small and large intestinal cells of horses. Twelve horses, euthanized for reasons unrelated to any gastrointestinal disease or systemic disease, were chosen for the study.
  • The team of researchers collected intestinal biopsy specimens through sharp dissection immediately following the euthanasia of the horses.
  • The specimens were subjected to immunohistochemical, immunofluorescence, and transmission electron microscopic imaging to detect and characterize the different types of epithelial cells present in them.
  • The team chose antibodies for cellular identification based on their expression in other mammalian species. These antibodies were intended to help them identify biomarkers for the various cell types.

Results of the Research

  • Immunostaining and morphological characterization via transmission electron microscopy were used to identify the types of intestinal epithelial cells.
  • The results indicated some differences in biomarker expression and antibody cross-reactivity in equine tissue when compared with other species.
  • Despite these differences, the team was able to identify each known type of mucosal epithelial cell in equine tissue.

Conclusions Drawn from the Research

  • The researchers concluded that the methodology implemented could potentially enhance the detection of stem cells and progenitor cells, as well as postmitotic cell lineages in equine intestinal tissues.
  • The research findings might have significant implications for the regenerative potential of intestinal mucosa and the survival of horses dealing with colic.

Cite This Article

APA
Gonzalez LM, Kinnin LA, Blikslager AT. (2015). Characterization of discrete equine intestinal epithelial cell lineages. Am J Vet Res, 76(4), 358-366. https://doi.org/10.2460/ajvr.76.4.358

Publication

American journal of veterinary research
ISSN: 1943-5681
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 76
Issue: 4
Pages: 358-366

Researcher Affiliations

Gonzalez, Liara M
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27502.
Kinnin, Leslie A
    Blikslager, Anthony T

      MeSH Terms

      • Animals
      • Cell Lineage
      • Colic / pathology
      • Colic / veterinary
      • Epithelial Cells / cytology
      • Female
      • Horse Diseases / pathology
      • Horses
      • Intestinal Mucosa / cytology

      Grant Funding

      • P30 DK034987 / NIDDK NIH HHS
      • T32 OD011130 / NIH HHS
      • T32OD011130 / NIH HHS

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      Citations

      This article has been cited 9 times.
      1. Cui C, Li L, Wu L, Wang X, Zheng Y, Wang F, Wei H, Peng J. Paneth cells in farm animals: current status and future direction. J Anim Sci Biotechnol 2023 Aug 15;14(1):118.
        doi: 10.1186/s40104-023-00905-5pubmed: 37582766google scholar: lookup
      2. Veerasammy B, Gonzalez G, Báez-Ramos P, Schaaf CR, Stewart AS, Ludwig EK, McKinney-Aguirre C, Freund J, Robertson J, Gonzalez LM. Changes in equine intestinal stem/progenitor cell number at resection margins in cases of small intestinal strangulation. Equine Vet J 2023 Jan 30;.
        doi: 10.1111/evj.13927pubmed: 36716291google scholar: lookup
      3. Coleman MC, Whitfield-Cargile C, Cohen ND, Goldsby JL, Davidson L, Chamoun-Emanuelli AM, Ivanov I, Eades S, Ing N, Chapkin RS. Non-invasive evaluation of the equine gastrointestinal mucosal transcriptome. PLoS One 2020;15(3):e0229797.
        doi: 10.1371/journal.pone.0229797pubmed: 32176710google scholar: lookup
      4. Fletcher OJ, Mansell R, Martin MP, Borst LB, Barnes HJ, Gonzalez LM. Gross Morphometry, Histomorphometry, and Immunohistochemistry Confirm Early and Persistent Jejunal Crypt Hyperplasia in Poults with Enteritis and Depressed Growth. Avian Dis 2018 Jun;62(2):163-170.
        doi: 10.1637/11759-101717-Reg.1pubmed: 29944394google scholar: lookup
      5. Blikslager A, Gonzalez L. Equine Intestinal Mucosal Pathobiology. Annu Rev Anim Biosci 2018 Feb 15;6:157-175.
        doi: 10.1146/annurev-animal-030117-014748pubmed: 29144770google scholar: lookup
      6. Kucera CR, Stranahan LW, Hughes F, Blikslager AT, Gonzalez LM. Protein biomarker of cell proliferation determines survival to discharge in cases of equine large colon volvulus. Equine Vet J 2018 Jul;50(4):452-456.
        doi: 10.1111/evj.12767pubmed: 29032573google scholar: lookup
      7. Stewart AS, Freund JM, Gonzalez LM. Advanced three-dimensional culture of equine intestinal epithelial stem cells. Equine Vet J 2018 Mar;50(2):241-248.
        doi: 10.1111/evj.12734pubmed: 28792626google scholar: lookup
      8. Stewart AS, Kopper JJ, McKinney-Aguirre C, Veerasamy B, Sahoo DK, Freund JM, Gonzalez LM. Assessment of equine intestinal epithelial junctional complexes and barrier permeability using a monolayer culture system. Front Vet Sci 2024;11:1455262.
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      9. Windhaber C, Heckl A, Csukovich G, Pratscher B, Burgener IA, Biermann N, Dengler F. A matter of differentiation: equine enteroids as a model for the in vivo intestinal epithelium. Vet Res 2024 Mar 16;55(1):30.
        doi: 10.1186/s13567-024-01283-0pubmed: 38493107google scholar: lookup
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