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Home / Equine Research Bank / J Vet Med Sci / Characterization of equine hyalocytes: their immunohistochem...
The Journal of veterinary medical science2016; 78(6); 937-942; doi: 10.1292/jvms.15-0511

Characterization of equine hyalocytes: their immunohistochemical properties, morphologies and distribution.

Abstract: In horse, the characterizations of hyalocytes under the steady state are still unclear. Therefore, we investigated characterizations of hyalocytes in normal equine eyes by their immunohistochemical phenotype, histomorphology and distribution. Thirty-one eyes from 18 horses, divided into 4 groups (G) by age, were used: early (G1) and late gestation (G2) fetuses, 1- to 3-year-old (G3) and 8- to 24-year-old (G4) horses. Equine hyalocytes were histologically classified into 4 types, and they immunohistochemically expressed MHC II and CD163. Hyalocytes were detected on and/or around ciliary processes and pars plana in G2, G3 and G4, but were not located on retina and optic papilla. A significant increase in distribution was found between G2 and both G3 and G4, and the largest distribution was found at ciliary processes in these groups. Equine hyalocytes were characterized as residential ocular macrophage and MHC II antigen-bearing cell, accompanied by a pleomorphic appearance and located in the contiguous ciliary body. Our data provided characterizations of hyalocytes in normal equine eyes and may well contribute to improving the understanding of pathogenesis of equine ocular disease.
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Publication Date: 2016-02-18 PubMed ID: 26888584PubMed Central: PMC4937152DOI: 10.1292/jvms.15-0511Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examined the properties, forms, and distribution of hyalocytes (a type of cell) in healthy horse eyes relating to their immunological signature, morphology, and placement, with an aim to better understand equine eye diseases.

Methodology

  • This study used a sample size of 31 eyes from 18 horses, which were divided into four age-based groups: early and late-stage fetuses (G1 and G2), one to three years old (G3), and eight to 24 years old (G4).
  • The researchers conducted an immunohistochemical analysis – a process used to detect antigens (proteins that stimulate an immune response) in cells of a tissue section – and a morphological examination on these samples, particularly focusing on the eyes’ hyalocytes.

Findings

  • The hyalocytes in horse eyes were categorized into four distinct types based on their morphology.
  • These cells expressed Major Histocompatibility Complex II (MHC II) and CD163, both of these are proteins significant in immune response and often used as markers to identify certain types of cells.
  • Hyalocytes were found around or on the ciliary processes and pars plana (parts of the eye) in G2, G3, and G4 horse groups, but they weren’t located on the retina and optic papilla (the part where the optic nerve enters the eyeball).
  • There was a significant increase in hyalocyte distribution between late-stage fetuses and the two groups of older horses, with the largest distribution found at the ciliary processes.
  • Hyalocytes were classified as residential ocular macrophages, meaning they reside permanently in the eye and work as part of the immune defence system. They appeared pleomorphic, signifying they had various forms, and were located mainly in the ciliary body (the part of the eye that produces the transparent liquid within the eye).

Implications

  • The findings provide valuable insight into the properties and distribution of hyalocytes in normal horse eyes.
  • Understanding the normal behavior and placement of these cells could contribute to improving the comprehension of the pathogenesis―the biological mechanism that leads to a disease―of equine ocular diseases, perhaps eventually leading to better diagnostic and therapeutic approaches.

Cite This Article

APA
Sano Y, Matsuda K, Okamoto M, Takehana K, Hirayama K, Taniyama H. (2016). Characterization of equine hyalocytes: their immunohistochemical properties, morphologies and distribution. J Vet Med Sci, 78(6), 937-942. https://doi.org/10.1292/jvms.15-0511

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 78
Issue: 6
Pages: 937-942

Researcher Affiliations

Sano, Yuto
  • Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Matsuda, Kazuya
    Okamoto, Minoru
      Takehana, Kazushige
        Hirayama, Kazuko
          Taniyama, Hiroyuki

            MeSH Terms

            • Age Factors
            • Animals
            • Female
            • Fluorescent Antibody Technique, Indirect / veterinary
            • Horses / anatomy & histology
            • Horses / embryology
            • Major Histocompatibility Complex / immunology
            • Male
            • Vitreous Body / cytology
            • Vitreous Body / ultrastructure

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            Citations

            This article has been cited 2 times.
            1. Nuzzi R, Bergandi L, Zabetta LC, D'Errico L, Riscaldino F, Menegon S, Silvagno F. In vitro generation of primary cultures of human hyalocytes. Mol Vis 2020;26:818-829.
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