Characterization of immunogenic proteins of Trypanosoma evansi isolated from three different Indian hosts using hyperimmune sera and immune sera.
Abstract: The western blot analysis for identification of immunogenic proteins in whole cell lysate (WCL) antigens (Ags) prepared from the Trypanosoma evansi of buffalo, horse and cattle origins using hyperimmune sera (HIS) showed 11 immunogenic proteins and naturally T. evansi infected immune sera (IS) of horse detected 19 immunogenic proteins. HIS and IS of horse recognized five common immunogenic proteins of relative molecular weight (M(r)) ranges 61-64, 44-47, 33-34, 25-26 and 14-16 kilo Dalton (kDa). HIS rose against WCL Ags of T. evansi of buffalo origin and immune sera of horse cross reacted with WCL Ags of T. evansi of different host origin. It can be concluded that in comparison to HIS, IS of horse could able to detect more numbers of immunogenic proteins and five common immunogenic proteins in WCL Ages of T. evansi of different hosts origin. The evidence of higher reactivity of IS in comparison to HIS against T. evansi is being reported for the first time.
Publication Date: 2008-03-26 PubMed ID: 18371998DOI: 10.1016/j.rvsc.2008.02.011Google Scholar: Lookup
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- Journal Article
Summary
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This research article presents the identification of immune-response triggering proteins in Trypanosoma evansi, a parasite causing disease in livestock, by using different types of immune sera from three different animal hosts (buffalo, horse, and cattle). The study contributes to the understanding of host-parasite interaction and it is the first to report a higher reactivity of naturally infected horse immune sera compared to hyperimmune sera against the parasite.
Objectives and Methods
- The main goal of the study was to identify and characterize specific proteins (immunogenic proteins) in Trypanosoma evansi that induce an immune response in different hosts: buffalo, horse, and cattle.
- The identification was done using western blot analysis, a common technique in molecular biology used to detect specific proteins in a sample.
- The whole cell lysate (WCL) antigens of T. evansi from the three different hosts were prepared, and then exposed to both hyperimmune sera (HIS) and immune sera (IS) from a horse.
Findings
- The researchers identified 11 immunogenic proteins when using hyperimmune sera (HIS), and 19 immunogenic proteins when using naturally infected immune sera (IS) from the horse.
- Within these, five common immunogenic proteins were recognized in both HIS and IS of the horse with molecular weights ranging between 14-16, 25-26, 33-34, 44-47, and 61-64 kilo Dalton (kDa).
- A cross-reactivity was noted between the immune sera of the horse and the antigens of T. evansi from different hosts. This means that the horse’s immune system responded to the same proteins in parasites from different animal hosts.
Conclusion and Significance
- The study concluded that naturally infected horse immune sera (IS) could detect more immunogenic proteins than the hyperimmune sera (HIS). This shows higher reactivity of the IS against T. evansi and it is reported for the first time in this research.
- This could be significant in developing better diagnostic tools and vaccines against T. evansi, since understanding which proteins trigger immune response are crucial for these advancements.
Cite This Article
APA
Laha R, Sasmal NK.
(2008).
Characterization of immunogenic proteins of Trypanosoma evansi isolated from three different Indian hosts using hyperimmune sera and immune sera.
Res Vet Sci, 85(3), 534-539.
https://doi.org/10.1016/j.rvsc.2008.02.011 Publication
Researcher Affiliations
- Indian Veterinary Research Institute, Kolkata 700 037, West Bengal, India.
MeSH Terms
- Animals
- Antigens, Protozoan / blood
- Antigens, Protozoan / immunology
- Antigens, Protozoan / isolation & purification
- Buffaloes / parasitology
- Cattle
- Cattle Diseases / immunology
- Cattle Diseases / parasitology
- Cross Reactions
- Electrophoresis, Polyacrylamide Gel
- Flagella / immunology
- Horse Diseases / immunology
- Horse Diseases / parasitology
- Horses
- India
- Species Specificity
- Trypanosoma / immunology
- Trypanosomiasis / diagnosis
- Trypanosomiasis / immunology
- Trypanosomiasis / veterinary
Citations
This article has been cited 2 times.- Reck C, Menin Á, Batista F, Meira Santos PO, Miletti LC. Evaluation of buffered Trypanosoma evansi antigen and rapid serum agglutination test (BA/Te) for the detection of anti-T. evansi antibodies in horses in Brazil. Curr Res Parasitol Vector Borne Dis 2021;1:100024.
- Sharma D, Gupta S, Sethi K, Kumar S, Kumar R. Seroprevalence and immunological characterization of Trypanosoma evansi infection in livestock of four agro-climatic zones of Himachal Pradesh, India. Trop Anim Health Prod 2022 Jan 15;54(1):60.
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