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Veterinary research2009; 41(2); 18; doi: 10.1051/vetres/2009066

Characterization of pentraxin 3 in the horse and its expression in airways.

Abstract: The long pentraxin 3 (PTX3) plays an important role in host defence and its over-expression may contribute to airway injury. The aim of the present study was therefore to characterize in more detail PTX3 and its expression in the horses' airway. Six healthy horses and six horses affected by recurrent airway obstruction (R.A.O.) were submitted to a dusty environment challenge. PTX3 DNA and cDNA were cloned and sequenced. PTX3 expression was evaluated by RT-qPCR, Western blotting and immunohistochemistry in bronchoalveolar lavage fluid (BALF) cells, BALF supernatant and bronchial epithelial cells. An alternative splicing of the second exon of PTX3 occurred, resulting in two forms of the protein: "spliced" (32 kDa) and "full length" (42 kDa). PTX3 was detected in BALF macrophages, neutrophils and bronchial epithelial cells. It was over-expressed in the BALF supernatant from R.A.O.-affected horses in crisis. However, dust was unable to induce PTX3 in BALF cells ex vivo, indicating that dust is an indirect inducer of PTX3. Dust exposure in-vivo induced PTX3 in BALF macrophages but there was no significant difference between healthy and R.A.O.-affected horses. Conversely, PTX3 was over-expressed in the bronchial epithelial cells from R.A.O.-affected horses in crisis. These data indicate a differential regulatory mechanism in inflammatory and bronchial epithelial cells and offer therapeutically interesting perspectives.
INRA, EDP Sciences, 2009
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Publication Date: 2009-10-29 PubMed ID: 19863902PubMed Central: PMC2789332DOI: 10.1051/vetres/2009066Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research was carried out to gain a better understanding of a protein called pentraxin 3 (PTX3) and its role in horses’ airways, particularly in relation to lung diseases like recurrent airway obstruction (R.A.O.) The study found that this protein occurred in two forms and was found in certain key cells in horses’ airways. Interestingly, it was over-produced in horses with R.A.O during flare-ups of the condition, suggesting it might have a role in this illness.

Study Objective and Methodology

  • The research primarily set out to analyze PTX3, its different forms and its presence in horses’ lungs. The study was initiated based on the understanding that PTX3 plays a crucial role in defending the host and its over-production could potentially cause harm to the airways.
  • The subjects of this research were six healthy horses and six horses diagnosed with recurrent airway obstruction (R.A.O.), an equine respiratory disease. All the horses were exposed to a dusty environment, intended to create a challenging condition.
  • Different scientific techniques, including cloning and sequencing of PTX3 DNA and cDNA as well as RT-qPCR, Western blotting, and immunohistochemistry, were utilized to observe PTX3 expression in different cell types found in the bronchoalveolar lavage fluid (BALF) and bronchial epithelial cells in the horses’ lungs.

Findings

  • The researchers discovered that an alternative splicing of the second exon of PTX3 gave rise to two forms of the protein: a “spliced” form, and a “full length” form.
  • The protein was detected in macrophages, neutrophils and bronchial epithelial cells, from the samples obtained from the horses. These cell types are essential to the immune response and lung health in mammals.
  • PTX3 was found to be over-expressed in the BALF supernatant from R.A.O.-affected horses during a crisis, indicating a possible association with the disease.
  • The in-vivo dust exposure led to increased PTX3 production in BALF macrophages, but there was not a significant difference between healthy and R.A.O.-affected horses. This suggests that dust alone was not the trigger for PTX3 production.
  • In contrast, PTX3 was over-expressed in the bronchial epithelial cells from R.A.O.-affected horses in crisis, suggesting a possible disease-specific response in these cells.

Conclusion

  • The findings present a potential differential regulatory mechanism in inflammatory and bronchial epithelial cells. They suggest that PTX3 may play an important role in the response to airway disease in horses, particularly in recurrent airway obstruction (R.A.O.). This could pave the way for future therapeutic strategies based on manipulation of PTX3 expression.

Cite This Article

APA
Ramery E, Fievez L, Fraipont A, Bureau F, Lekeux P. (2009). Characterization of pentraxin 3 in the horse and its expression in airways. Vet Res, 41(2), 18. https://doi.org/10.1051/vetres/2009066

Publication

Veterinary research
ISSN: 0928-4249
NlmUniqueID: 9309551
Country: England
Language: English
Volume: 41
Issue: 2
Pages: 18
PII: 18

Researcher Affiliations

Ramery, Eve
  • Department for Functional Sciences, Faculty of Veterinary Medicine, University of Liege, Bvd de Colonster, 20, B-4000 Liege, Belgium. eve.ramery@ulg.ac.be
Fievez, Laurence
    Fraipont, Audrey
      Bureau, Fabrice
        Lekeux, Pierre

          MeSH Terms

          • Airway Obstruction / metabolism
          • Airway Obstruction / veterinary
          • Amino Acid Sequence
          • Animals
          • Bronchoalveolar Lavage Fluid / cytology
          • C-Reactive Protein / genetics
          • C-Reactive Protein / metabolism
          • Gene Expression Regulation / physiology
          • Horse Diseases / genetics
          • Horse Diseases / metabolism
          • Horses
          • Molecular Sequence Data
          • Serum Amyloid P-Component / genetics
          • Serum Amyloid P-Component / metabolism

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          Citations

          This article has been cited 2 times.
          1. Townsend M, Fowler B, Aulakh GK, Singh B. Expression of pentraxin 3 in equine lungs and neutrophils. Can J Vet Res 2023 Jan;87(1):9-16.
            pubmed: 36606044
          2. Korn A, Miller D, Dong L, Buckles EL, Wagner B, Ainsworth DM. Differential Gene Expression Profiles and Selected Cytokine Protein Analysis of Mediastinal Lymph Nodes of Horses with Chronic Recurrent Airway Obstruction (RAO) Support an Interleukin-17 Immune Response. PLoS One 2015;10(11):e0142622.
            doi: 10.1371/journal.pone.0142622pubmed: 26561853google scholar: lookup
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