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Home / Equine Research Bank / Molecules / Chemical Analysis of Lepidium meyenii (Maca) and Its Effects...
Molecules (Basel, Switzerland)2019; 24(10); 1981; doi: 10.3390/molecules24101981

Chemical Analysis of Lepidium meyenii (Maca) and Its Effects on Redox Status and on Reproductive Biology in Stallions †.

Abstract: The present study was conducted to assess the chemical composition of Yellow Maca () and its biological activity on stallions following oral administration of hypocotyl powder. Maca was subjected to methanolic extraction and the chemical analysis was carried out by LC-MS-QTOF (liquid chromatography-mass spectrometry). Our results showed that Maca contains some effective antioxidants, a high percentage of glucosinolates, and other important components with a high antioxidant capacity. To evaluate the plant biological activity in stallions fed with Maca powder for 60 days, the redox status and some reproductive parameters were investigated. Blood and semen samples were collected at 0, 30, 60, and 90 days from the beginning of this study. Blood samples showed a decrease of the reactive oxygen metabolites, evaluated by d-ROMs test, and an increase of the antioxidant barrier in terms of biological antioxidant potential (BAP test), powerful oxidant capacity (OXY-Adsorbent test), and thiols evaluation (-SHp test). Furthermore, semen samples showed a positive trend during Maca administration in the following parameters: ejaculate volumes and sperm concentrations, total and progressive motility, and acrosome integrity.
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Publication Date: 2019-05-23 PubMed ID: 31126050PubMed Central: PMC6571579DOI: 10.3390/molecules24101981Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the chemical composition of a plant called Yellow Maca and its influence on the reproductive biology and oxidative stress in stallions after they consume the plant’s ground-up part called the hypocotyl for 60 days.

Chemical Analysis of Yellow Maca

  • The study began with an analysis of Yellow Maca’s chemical composition using methanolic extraction and LC-MS-QTOF, a method that combines liquid chromatography and mass spectrometry.
  • The analysis discovered that Maca is rich in powerful antioxidants along with a high percentage of glucosinolates. Antioxidants prevent oxidative damage in the body, and glucosinolates are natural components found in many pungent plants like mustard, cabbage, and horseradish, which may have cancer-preventative properties.

Biological Activity of Yellow Maca in Stallions

  • The biological activity of Maca was evaluated on stallions that were fed with Maca powder for a period of 60 days. This analysis aimed to understand the plant’s effect on the animals’ redox status and various reproductive parameters.
  • The redox status reflects the balance between oxidants and antioxidants in the body. A change in this balance towards more oxidants can lead to a condition known as oxidative stress, damaging cells and tissues.
  • Blood and semen samples were collected from the stallions at the beginning of the experiment and then again after 30, 60, and 90 days.

Effects on Redox Status

  • The results confirmed that consuming Maca led to a decrease in reactive oxygen metabolites as per the d-ROMs test, suggesting a decrease in oxidative stress.
  • Simultaneously, the antioxidant barrier, as evaluated by the biological antioxidant potential (BAP) test, the OXY-Adsorbent test (which validates the body’s capacity to counteract oxidants), and the thiols (-SHp) test (which measures antioxidant molecules containing sulfur), increased, showing an improvement in the body’s antioxidant defenses.

Effects on Reproductive Biology

  • The study also found that semen samples demonstrated a positive trend during the administration of Maca.
  • The parameters that showed improvement included ejaculate volumes and sperm concentrations, total and progressive motility of sperm, and acrosome integrity. The acrosome is a cap-like structure over the anterior part of a spermatozoon’s head, whose rupture or reaction is necessary for the sperm to penetrate the egg and achieve fertilization.

Cite This Article

APA
Tafuri S, Cocchia N, Carotenuto D, Vassetti A, Staropoli A, Mastellone V, Peretti V, Ciotola F, Albarella S, Del Prete C, Palumbo V, Esposito L, Vinale F, Ciani F. (2019). Chemical Analysis of Lepidium meyenii (Maca) and Its Effects on Redox Status and on Reproductive Biology in Stallions †. Molecules, 24(10), 1981. https://doi.org/10.3390/molecules24101981

Publication

Molecules (Basel, Switzerland)
ISSN: 1420-3049
NlmUniqueID: 100964009
Country: Switzerland
Language: English
Volume: 24
Issue: 10
PII: 1981

Researcher Affiliations

Tafuri, Simona
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy. stafuri@unina.it.
Cocchia, Natascia
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy. ncocchia@unina.it.
Carotenuto, Domenico
  • UNMSM, Universidad Nacional Mayor San Marcos, Lima 11-0058, Peru. domenicar@alice.it.
Vassetti, Anastasia
  • Institute for Sustainable Plant Protection, National Research Council, 80055 Portici (Na), Italy. anastasia.vassetti@ipsp.cnr.it.
Staropoli, Alessia
  • Institute for Sustainable Plant Protection, National Research Council, 80055 Portici (Na), Italy. alessia.staropoli@unina.it.
  • Department of Agricultural Sciences, University of Naples Federico II, 80055 Naples, Italy. alessia.staropoli@unina.it.
Mastellone, Vincenzo
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy. vincenzo.mastellone@unina.it.
Peretti, Vincenzo
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy. vincenzo.peretti@unina.it.
Ciotola, Francesca
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy. francesca.ciotola@unina.it.
Albarella, Sara
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy. sara.albarella@unina.it.
Del Prete, Chiara
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy. chiara.delprete@unina.it.
Palumbo, Veronica
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy. veronica.palumbo@unina.it.
Esposito, Luigi
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy. luigespo@unina.it.
Vinale, Francesco
  • Institute for Sustainable Plant Protection, National Research Council, 80055 Portici (Na), Italy. francesco.vinale@ipsp.cnr.it.
  • Department of Agricultural Sciences, University of Naples Federico II, 80055 Naples, Italy. francesco.vinale@ipsp.cnr.it.
Ciani, Francesca
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy. ciani@unina.it.

MeSH Terms

  • Administration, Oral
  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / chemistry
  • Antioxidants / pharmacology
  • Gas Chromatography-Mass Spectrometry
  • Glucosinolates / administration & dosage
  • Glucosinolates / chemistry
  • Glucosinolates / pharmacology
  • Horses
  • Hypocotyl / chemistry
  • Lepidium / chemistry
  • Male
  • Oxidation-Reduction / drug effects
  • Phytochemicals / administration & dosage
  • Phytochemicals / chemistry
  • Phytochemicals / pharmacology
  • Powders / administration & dosage
  • Reactive Oxygen Species / blood
  • Semen / drug effects
  • Semen / physiology
  • Sperm Motility / drug effects

Conflict of Interest Statement

None of the authors of this article has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the article.

References

This article includes 38 references
  1. Gonzales GF, Ruiz A, Gonzales C, Villegas L, Cordova A. Effect of Lepidium meyenii (maca) roots on spermatogenesis of male rats.. Asian J Androl 2001 Sep;3(3):231-3.
    pubmed: 11561196
  2. Canales M, Aguilar J, Prada A, Marcelo A, Huamán C, Carbajal L. [Nutritional evaluation of Lepidium meyenii (MACA) in albino mice and their descendants].. Arch Latinoam Nutr 2000 Jun;50(2):126-33.
    pubmed: 11048583
  3. Gonzales GF. Ethnobiology and Ethnopharmacology of Lepidium meyenii (Maca), a Plant from the Peruvian Highlands.. Evid Based Complement Alternat Med 2012;2012:193496.
    doi: 10.1155/2012/193496pmc: PMC3184420pubmed: 21977053google scholar: lookup
  4. Gonzales GF, Miranda S, Nieto J, Fernández G, Yucra S, Rubio J, Yi P, Gasco M. Red maca (Lepidium meyenii) reduced prostate size in rats.. Reprod Biol Endocrinol 2005 Jan 20;3:5.
    pmc: PMC548136pubmed: 15661081doi: 10.1186/1477-7827-3-5google scholar: lookup
  5. Oshima M, Gu Y, Tsukada S. Effects of Lepidium meyenii Walp and Jatropha macrantha on blood levels of estradiol-17 beta, progesterone, testosterone and the rate of embryo implantation in mice.. J Vet Med Sci 2003 Oct;65(10):1145-6.
    doi: 10.1292/jvms.65.1145pubmed: 14600359google scholar: lookup
  6. Valentová K, Buckiová D, Kren V, Peknicová J, Ulrichová J, Simánek V. The in vitro biological activity of Lepidium meyenii extracts.. Cell Biol Toxicol 2006 Mar;22(2):91-9.
    doi: 10.1007/s10565-006-0033-0pubmed: 16528448google scholar: lookup
  7. Gonzales GF, Cordova A, Gonzales C, Chung A, Vega K, Villena A. Lepidium meyenii (Maca) improved semen parameters in adult men.. Asian J Androl 2001 Dec;3(4):301-3.
    pubmed: 11753476
  8. Gonzales GF, Gasco M, Córdova A, Chung A, Rubio J, Villegas L. Effect of Lepidium meyenii (Maca) on spermatogenesis in male rats acutely exposed to high altitude (4340 m).. J Endocrinol 2004 Jan;180(1):87-95.
    doi: 10.1677/joe.0.1800087pubmed: 14709147google scholar: lookup
  9. Del Prete C, Cocchia N, Ciani F, Tafuri S, Carotenuto D, Napoleone G, Pasolini MP. Influence of a diet supplementation with Lepidium meyenii on quality of cooled equine semen.. Reprod. Domest. Anim. 2015;50:30.
    doi: 10.1111/rda.12582google scholar: lookup
  10. Del Prete C, Tafuri S, Ciani F, Pasolini MP, Ciotola F, Albarella S, Carotenuto D, Peretti V, Cocchia N. Influences of dietary supplementation with Lepidium meyenii (Maca) on stallion sperm production and on preservation of sperm quality during storage at 5 °C.. Andrology 2018 Mar;6(2):351-361.
    doi: 10.1111/andr.12463pubmed: 29359412google scholar: lookup
  11. Ciani F, Cocchia N, d’Angelo D, Tafuri S. Influence of ROS on Ovarian Functions.. In: Wu B., editor. New Discoveries in Embryology. Intechopen; London, UK: 2015. pp. 41–73.
    doi: 10.5772/61003google scholar: lookup
  12. Mathur PP, D'Cruz SC. The effect of environmental contaminants on testicular function.. Asian J Androl 2011 Jul;13(4):585-91.
    doi: 10.1038/aja.2011.40pmc: PMC3739630pubmed: 21706039google scholar: lookup
  13. Tafuri S, Ciani F, Iorio EL, Esposito L, Cocchia N. Reactive oxygen species (ROS) and male fertility.. In: Wu B., editor. New Discoveries in Embryology. Intechopen; London, UK: 2015. pp. 19–40.
    doi: 10.5772/60632google scholar: lookup
  14. Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility.. Hum Reprod Update 2003 Jul-Aug;9(4):331-45.
    doi: 10.1093/humupd/dmg027pubmed: 12926527google scholar: lookup
  15. Sies H, Mehlhorn R. Mutagenicity of nitroxide-free radicals.. Arch Biochem Biophys 1986 Nov 15;251(1):393-6.
    doi: 10.1016/0003-9861(86)90087-1pubmed: 3539021google scholar: lookup
  16. Costantino M, Giuberti G, Caraglia M, Lombardi A, Misso G, Abbruzzese A, Ciani F, Lampa E. Possible antioxidant role of SPA therapy with chlorine-sulphur-bicarbonate mineral water.. Amino Acids 2009 Feb;36(2):161-5.
    doi: 10.1007/s00726-008-0032-ypubmed: 18288460google scholar: lookup
  17. Prete CD, Ciani F, Tafuri S, Pasolini MP, Valle GD, Palumbo V, Abbondante L, Calamo A, Barbato V, Gualtieri R, Talevi R, Cocchia N. Effect of superoxide dismutase, catalase, and glutathione peroxidase supplementation in the extender on chilled semen of fertile and hypofertile dogs.. J Vet Sci 2018 Sep 30;19(5):667-675.
    doi: 10.4142/jvs.2018.19.5.667pmc: PMC6167334pubmed: 29649854google scholar: lookup
  18. Zhou Y, Li P, Brantner A, Wang H, Shu X, Yang J, Si N, Han L, Zhao H, Bian B. Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients.. Sci Rep 2017 Mar 17;7:44660.
    doi: 10.1038/srep44660pmc: PMC5356334pubmed: 28304399google scholar: lookup
  19. Vinale F, Strakowska J, Mazzei P, Piccolo A, Marra R, Lombardi N, Manganiello G, Pascale A, Woo SL, Lorito M. Cremenolide, a new antifungal, 10-member lactone from Trichoderma cremeum with plant growth promotion activity.. Nat Prod Res 2016 Nov;30(22):2575-2581.
    doi: 10.1080/14786419.2015.1131985pubmed: 26728227google scholar: lookup
  20. Vinale F, Nicoletti R, Lacatena F, Marra R, Sacco A, Lombardi N, d'Errico G, Digilio MC, Lorito M, Woo SL. Secondary metabolites from the endophytic fungus Talaromyces pinophilus.. Nat Prod Res 2017 Aug;31(15):1778-1785.
    doi: 10.1080/14786419.2017.1290624pubmed: 28278635google scholar: lookup
  21. Bennett RN, Mellon FA, Kroon PA. Screening crucifer seeds as sources of specific intact glucosinolates using ion-pair high-performance liquid chromatography negative ion electrospray mass spectrometry.. J Agric Food Chem 2004 Feb 11;52(3):428-38.
    doi: 10.1021/jf030530ppubmed: 14759128google scholar: lookup
  22. Feng J, Dou J, Wu Z, Yin D, Wu W. Controlled Release of Biological Control Agents for Preventing Aflatoxin Contamination from Starch⁻Alginate Beads.. Molecules 2019 May 14;24(10).
    doi: 10.3390/molecules24101858pmc: PMC6572238pubmed: 31091816google scholar: lookup
  23. Fahey JW, Zalcmann AT, Talalay P. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants.. Phytochemistry 2001 Jan;56(1):5-51.
    doi: 10.1016/S0031-9422(00)00316-2pubmed: 11198818google scholar: lookup
  24. Gonzales GF, Córdova A, Vega K, Chung A, Villena A, Góñez C, Castillo S. Effect of Lepidium meyenii (MACA) on sexual desire and its absent relationship with serum testosterone levels in adult healthy men.. Andrologia 2002 Dec;34(6):367-72.
    doi: 10.1046/j.1439-0272.2002.00519.xpubmed: 12472620google scholar: lookup
  25. Zheng BL, He K, Kim CH, Rogers L, Shao Y, Huang ZY, Lu Y, Yan SJ, Qien LC, Zheng QY. Effect of a lipidic extract from lepidium meyenii on sexual behavior in mice and rats.. Urology 2000 Apr;55(4):598-602.
    doi: 10.1016/S0090-4295(99)00549-Xpubmed: 10736519google scholar: lookup
  26. Cicero AF, Bandieri E, Arletti R. Lepidium meyenii Walp. improves sexual behaviour in male rats independently from its action on spontaneous locomotor activity.. J Ethnopharmacol 2001 May;75(2-3):225-9.
    doi: 10.1016/S0378-8741(01)00195-7pubmed: 11297856google scholar: lookup
  27. Cocchia N, Ciani F, El-Rass R, Russo M, Borzacchiello G, Esposito V, Montagnaro S, Avallone L, Tortora G, Lorizio R. Cryopreservation of feline epididymal spermatozoa from dead and alive animals and its use in assisted reproduction.. Zygote 2010 Feb;18(1):1-8.
    doi: 10.1017/S0967199409990256pubmed: 19939333google scholar: lookup
  28. Cocchia N, Corteggio A, Altamura G, Tafuri S, Rea S, Rosapane I, Sica A, Landolfi F, Ciani F. The effects of superoxide dismutase addition to the transport medium on cumulus-oocyte complex apoptosis and IVF outcome in cats (Felis catus).. Reprod Biol 2015 Mar;15(1):56-64.
    doi: 10.1016/j.repbio.2014.10.002pubmed: 25726378google scholar: lookup
  29. Tafuri S, Marullo A, Ciani F, Della Morte R, Montagnaro S, Fiorito F, De Martino L. Reactive oxygen metabolites in alpha-herpesvirus-seropositive Mediterranean buffaloes (Bubalus bubalis): a preliminary study.. Pol J Vet Sci 2018 Sep;21(3):639-642.
    pubmed: 30468352doi: 10.24425/124300google scholar: lookup
  30. Alberti A, Bolognini L, Macciantelli D, Carratelli M. The radical cation of N,N-diethylpara-phenylendiamine: A possible indicator of oxidative stress in biological samples.. Res. Chem. Intermediat. 2000;26:253–267.
    doi: 10.1163/156856700X00769google scholar: lookup
  31. Muhammad I, Zhao J, Khan IA. Maca (Lepidium meyenii). In: Coates P., Blackman M.R., Cragg G., Levine M., Moss J., White J., editors. Encyclopedia of Dietary Supplement. 2nd ed. Supplement 1. Markel Dekker Inc.; New York, NY, USA: 2010. pp. 522–531.
    doi: 10.1081/E-EDS-120022117google scholar: lookup
  32. Piacente S, Carbone V, Plaza A, Zampelli A, Pizza C. Investigation of the tuber constituents of maca (Lepidium meyenii Walp.).. J Agric Food Chem 2002 Sep 25;50(20):5621-5.
    doi: 10.1021/jf020280xpubmed: 12236688google scholar: lookup
  33. Esparza E, Hadzich A, Kofer W, Mithöfer A, Cosio EG. Bioactive maca (Lepidium meyenii) alkamides are a result of traditional Andean postharvest drying practices.. Phytochemistry 2015 Aug;116:138-148.
    doi: 10.1016/j.phytochem.2015.02.030pubmed: 25817836google scholar: lookup
  34. Zhao J, Muhammad I, Dunbar DC, Mustafa J, Khan IA. New alkamides from maca (Lepidium meyenii).. J Agric Food Chem 2005 Feb 9;53(3):690-3.
    doi: 10.1021/jf048529tpubmed: 15686421google scholar: lookup
  35. Chain FE, Grau A, Martins JC, Catalan CAN. Macamides from wild “Maca”, Lepidium meyenii Walpers (Brassicaceae). Phytochem. Lett. 2014;8:145–148.
    doi: 10.1016/j.phytol.2014.03.005google scholar: lookup
  36. Martínez-Soto JC, Landeras J, Gadea J. Spermatozoa and seminal plasma fatty acids as predictors of cryopreservation success.. Andrology 2013 May;1(3):365-75.
    doi: 10.1111/j.2047-2927.2012.00040.xpubmed: 23596043google scholar: lookup
  37. Fazio F, Casella S, Giannetto C, Caola G, Piccione G. Serum homocysteine and oxidative stress evaluation during exercise in horse.. Pol J Vet Sci 2009;12(2):169-74.
    pubmed: 19645345
  38. Melnikovova I, Fait T, Kolarova M, Fernandez EC, Milella L. Effect of Lepidium meyenii Walp. on Semen Parameters and Serum Hormone Levels in Healthy Adult Men: A Double-Blind, Randomized, Placebo-Controlled Pilot Study.. Evid Based Complement Alternat Med 2015;2015:324369.
    pmc: PMC4569766pubmed: 26421049doi: 10.1155/2015/324369google scholar: lookup

Citations

This article has been cited 24 times.
  1. Levano G, Quispe J, Vargas D, García M, López A, Aguila L, Valdivia M. Effect of Atomized Black Maca (Lepidium meyenii) Supplementation in the Cryopreservation of Alpaca (Vicugna pacos) Epididymal Spermatozoa. Animals (Basel) 2023 Jun 21;13(13).
    doi: 10.3390/ani13132054pubmed: 37443852google scholar: lookup
  2. Lee HW, Lee MS, Qu F, Lee JW, Kim E. Maca (Lepidium meyenii Walp.) on semen quality parameters: A systematic review and meta-analysis. Front Pharmacol 2022;13:934740.
    doi: 10.3389/fphar.2022.934740pubmed: 36110519google scholar: lookup
  3. Leiva-Revilla J, Rolón M, Siyadatpanah A, Pereira ML, Nissapatorn V. First study of in vitro protective effect of Lepidium meyenii (Maca) on frozen-thawed bovine spermatozoa. Vet World 2022 Jun;15(6):1481-1488.
    doi: 10.14202/vetworld.2022.1481-1488pubmed: 35993065google scholar: lookup
  4. Del Prete C, Calabria A, Longobardi V, Palumbo V, Merlo B, Iacono E, Tafuri S, Carotenuto D, Ciani F, Damiano S, Ciarcia R, Cocchia N. Effect of Aqueous Extract of Maca Addition to an Extender for Chilled Canine Semen. Animals (Basel) 2022 Jun 26;12(13).
    doi: 10.3390/ani12131638pubmed: 35804537google scholar: lookup
  5. Ibrahim RM, Elmasry GF, Refaey RH, El-Shiekh RA. Lepidium meyenii (Maca) Roots: UPLC-HRMS, Molecular Docking, and Molecular Dynamics. ACS Omega 2022 May 24;7(20):17339-17357.
    doi: 10.1021/acsomega.2c01342pubmed: 35647470google scholar: lookup
  6. Al-Khaldi K, Yimer N, Sadiq MB, Firdaus Jesse Bin Abdullah F, Salam Babji A, Al-Bulushi S. Edible bird's nest supplementation in chilled and cryopreserved Arabian stallion semen. Saudi J Biol Sci 2022 Mar;29(3):1577-1584.
    doi: 10.1016/j.sjbs.2021.11.009pubmed: 35280569google scholar: lookup
  7. Turgud FK, Narinç D. Influences of Dietary Supplementation with Maca (Lepidium meyenii) on Performance, Parameters of Growth Curve and Carcass Characteristics in Japanese Quail. Animals (Basel) 2022 Jan 28;12(3).
    doi: 10.3390/ani12030318pubmed: 35158642google scholar: lookup
  8. Greco A, Del Prete C, De Biase D, Palumbo V, Albanese S, Bruzzese D, Carotenuto D, Ciani F, Tafuri S, Meomartino L, Mancini M, Paciello O, Cocchia N. Effects of Oral Administration of Lepidium meyenii on Morphology of Mice Testis and Motility of Epididymal Sperm Cells After Tetrahydrocannabinol Exposure. Front Vet Sci 2021;8:692874.
    doi: 10.3389/fvets.2021.692874pubmed: 34957272google scholar: lookup
  9. Ciani F, Maruccio L, Cocchia N, d'Angelo D, Carotenuto D, Avallone L, Namagerdi AA, Tafuri S. Antioxidants in assisted reproductive technologies: An overview on dog, cat, and horse. J Adv Vet Anim Res 2021 Mar;8(1):173-184.
    doi: 10.5455/javar.2021.h500pubmed: 33860028google scholar: lookup
  10. Tirpák F, Greifová H, Lukáč N, Stawarz R, Massányi P. Exogenous Factors Affecting the Functional Integrity of Male Reproduction. Life (Basel) 2021 Mar 9;11(3).
    doi: 10.3390/life11030213pubmed: 33803103google scholar: lookup
  11. Bazzano M, Laus F, Spaterna A, Marchegiani A. Use of nutraceuticals in the stallion: Effects on semen quality and preservation. Reprod Domest Anim 2021 Jul;56(7):951-957.
    doi: 10.1111/rda.13934pubmed: 33772909google scholar: lookup
  12. Marra R, Coppola M, Pironti A, Grasso F, Lombardi N, d'Errico G, Sicari A, Bolletti Censi S, Woo SL, Rao R, Vinale F. The Application of Trichoderma Strains or Metabolites Alters the Olive Leaf Metabolome and the Expression of Defense-Related Genes. J Fungi (Basel) 2020 Dec 16;6(4).
    doi: 10.3390/jof6040369pubmed: 33339378google scholar: lookup
  13. Peretti V, Satué K, Ciotola F, Cristarella S, De Majo M, Biondi V, D'Anza E, Albarella S, Quartuccio M. An Unusual Case of Testicular Disorder in Sex Development of Arabian Mare (64,XX SRY-Negative). Animals (Basel) 2020 Oct 25;10(11).
    doi: 10.3390/ani10111963pubmed: 33113813google scholar: lookup
  14. Dini I, Graziani G, Fedele FL, Sicari A, Vinale F, Castaldo L, Ritieni A. An Environmentally Friendly Practice Used in Olive Cultivation Capable of Increasing Commercial Interest in Waste Products from Oil Processing. Antioxidants (Basel) 2020 Jun 1;9(6).
    doi: 10.3390/antiox9060466pubmed: 32492835google scholar: lookup
  15. Dini I, Graziani G, Gaspari A, Fedele FL, Sicari A, Vinale F, Cavallo P, Lorito M, Ritieni A. New Strategies in the Cultivation of Olive Trees and Repercussions on the Nutritional Value of the Extra Virgin Olive Oil. Molecules 2020 May 18;25(10).
    doi: 10.3390/molecules25102345pubmed: 32443449google scholar: lookup
  16. Quispe-Díaz IM, Ybañez-Julca RO, Asunción-Alvarez D, Enriquez-Lara C, Polo-Bardales JL, Jara-Aguilar R, Venegas-Casanova EA, de Albuquerque RDDG, Costilla-Sánchez N, Vásquez-Corales E, Buc Calderon P, Benites J. Antioxidant, Neuroprotective, and Antinociceptive Effects of Peruvian Black Maca (Lepidium meyenii Walp.). Antioxidants (Basel) 2025 Oct 8;14(10).
    doi: 10.3390/antiox14101214pubmed: 41154523google scholar: lookup
  17. Elsayed SA, Ibrahim RS, El Naggar EMB, Hassan YA, Shawky E. Serum metabolomics-driven network pharmacology elucidate the anti-rheumatoid arthritis potential of garden cress. Sci Rep 2025 Sep 1;15(1):32091.
    doi: 10.1038/s41598-025-13412-6pubmed: 40890195google scholar: lookup
  18. Biancani B, Carosi M, Capasso M, Rossi G, Tafuri S, Ciani F, Cotignoli C, Zinno F, Venturelli E, Galliani M, Spani F. Assessment of Oxidative Stress and Biometric Data in a Captive Colony of Hamadryas Baboons (Papio hamadryas Linnaeus, 1758) at the Ravenna Zoo Safari (Italy). Vet Sci 2025 May 13;12(5).
    doi: 10.3390/vetsci12050466pubmed: 40431559google scholar: lookup
  19. Gatta C, Iorio EL, Genovese C, Biancani B, Mores A, La Monaca D, Caterino C, Avallone L, Sanchez-Contreras GJ, De Vivo I, Ciani F, Tafuri S. Assessing Bottlenose Dolphins' (Tursiops truncatus) Health Status Through Functional Muscle Analysis, and Oxidative and Metabolic Stress Evaluation: A Preliminary Study. Animals (Basel) 2025 Apr 25;15(9).
    doi: 10.3390/ani15091215pubmed: 40362029google scholar: lookup
  20. Tóth S, Szlávik MF, Mandel R, Fekecs F, Tusnády G, Vajda F, Varga N, Apáti Á, Bényei A, Paczal A, Kotschy A, Szakács G. Synthesis and Systematic Investigation of Lepidiline A and Its Gold(I), Silver(I), and Copper(I) Complexes Using In Vitro Cancer Models and Multipotent Stem Cells. ACS Omega 2024 Jul 23;9(29):32226-32234.
    doi: 10.1021/acsomega.4c05020pubmed: 39072085google scholar: lookup
  21. Zaukuu JZ, Adams ZS, Donkor-Boateng NA, Mensah ET, Bimpong D, Amponsah LA. Non-invasive prediction of maca powder adulteration using a pocket-sized spectrophotometer and machine learning techniques. Sci Rep 2024 May 7;14(1):10426.
    doi: 10.1038/s41598-024-61220-1pubmed: 38714752google scholar: lookup
  22. Gattuso DT, Polisca A, Interlandi CD, Rizzo M, Tabbì M, Giudice E, Cristarella S, Rifici C, Quartuccio M, Zappone V. Influence of dietary supplementation with Lepidium meyenii (Maca) on sperm quality in dogs. Front Vet Sci 2024;11:1375146.
    doi: 10.3389/fvets.2024.1375146pubmed: 38487706google scholar: lookup
  23. Minich DM, Ross K, Frame J, Fahoum M, Warner W, Meissner HO. Not All Maca Is Created Equal: A Review of Colors, Nutrition, Phytochemicals, and Clinical Uses. Nutrients 2024 Feb 14;16(4).
    doi: 10.3390/nu16040530pubmed: 38398854google scholar: lookup
  24. Folashade OP, Boyenle ID, Oyedeji TA, Ojeniyi FD, Damilare AA, Ehigie LO, Ehigie AF. Jatonik polyherbal mixture induced rat liver MMPT pore opening in normal Wistar rat: In vitro and in vivo studies. Chin Herb Med 2024 Jan;16(1):113-120.
    doi: 10.1016/j.chmed.2023.06.002pubmed: 38375053google scholar: lookup
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