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Home / Equine Research Bank / Vet Comp Orthop Traumatol / Chondrogenic potential of mesenchymal stromal cells derived ...
Veterinary and comparative orthopaedics and traumatology : V.C.O.T2009; 22(5); 363-370; doi: 10.3415/VCOT-08-10-0107

Chondrogenic potential of mesenchymal stromal cells derived from equine bone marrow and umbilical cord blood.

Abstract: Orthopaedic injury is the most common cause of lost training days or premature retirement in the equine athlete. Cell-based therapies are a potential new treatment option in musculo-skeletal diseases. Mesenchymal stromal cells (MSC) have been derived from multiple sources in the horse including bone marrow and umbilical cord blood. The objective of this study was to provide an in vitro comparison of the chondrogenic potential in MSC derived from adult bone marrow (BM-MSC) and umbilical cord blood (CB-MSC). Results: MSC from both sources produced tissue with cartilage-like morphology that stained positive for proteoglycans and expressed cartilage markers. The CB-MSC pellets were larger and showed hyaline-like cartilage morphology as early as day six. Gene expression of collagen type 21, aggrecan and CD-RAP was higher in CB- than BM-MSC pellets. Expression of Sox9 mRNA was similar between CB- and BM-MSC pellets. Protein concentration of cartilage-derived retinoic acid sensitive protein was higher in culture medium from CB- than BM-MSC pellets. Conclusions: CB-MSC and BM-MSC were both capable of producing hyaline-like cartilage in vitro . However, in this study the MSC from umbilical cord blood appeared to have more chondrogenic potential than the BM-MSC based on the cells tested and parameters measured.
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Publication Date: 2009-08-28 PubMed ID: 19750290DOI: 10.3415/VCOT-08-10-0107Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the potential of using cells from horse bone marrow and umbilical cord blood to create cartilage-like tissue, which could be a new treatment for equine athletes with orthopaedic injuries. The study found that cells from both sources could produce this type of tissue, but the cells from umbilical cord blood seemed to have more potential based on the measurements taken.

Research Objective

  • The aim of this study was to compare the chondrogenic potential (i.e., the ability to generate cartilage) of mesenchymal stromal cells derived from adult bone marrow (BM-MSC) and umbilical cord blood (CB-MSC). These cells have been identified in horses and are the subject of study for their possible application in treating musculoskeletal diseases.

Research Methods

  • The researchers compared the chondrogenic potential of BM-MSC and CB-MSC through an in vitro study. They examined whether these cells could produce tissue with cartilage-like morphology, if they stained positive for proteoglycans, and if they expressed cartilage markers.

Results

  • Both the MSC derived from bone marrow and from umbilical cord blood were found to produce tissue with cartilage-like morphology and expressed cartilage markers, indicating their ability to generate cartilage. The study found that MSC derived from umbilical cord blood formed larger pellets and demonstrated hyaline-like cartilage morphology more rapidly than the bone marrow-derived cells.
  • The researchers also examined the gene expression in the cell pellets. They found that the expression of Sox9 mRNA, a transcription factor crucial for cartilage formation, was similar in both BM-MSC and CB-MSC derived cells. However, the expression of other genes critical for cartilage function, namely collagen type 21, aggrecan and CD-RAP, were higher in CB-MSC than BM-MSC pellets.
  • Moreover, concentrations of cartilage-derived retinoic acid sensitive protein were found to be higher in the culture medium from CB-MSC than from BM-MSC pellets.

Conclusion

  • Both types of MSC, those derived from bone marrow and umbilical cord blood, exhibited the ability to produce hyaline-like cartilage in vitro . However, in this experiment the stromal cells from umbilical cord blood demonstrated more chondrogenic potential than those from bone-marrow based on the measures tested, suggesting that these cells could be a more effective option for cell-based therapies in equine musculoskeletal disease.

Cite This Article

APA
Berg L, Koch T, Heerkens T, Bessonov K, Thomsen P, Betts D. (2009). Chondrogenic potential of mesenchymal stromal cells derived from equine bone marrow and umbilical cord blood. Vet Comp Orthop Traumatol, 22(5), 363-370. https://doi.org/10.3415/VCOT-08-10-0107

Publication

Veterinary and comparative orthopaedics and traumatology : V.C.O.T
ISSN: 0932-0814
NlmUniqueID: 8906319
Country: Germany
Language: English
Volume: 22
Issue: 5
Pages: 363-370

Researcher Affiliations

Berg, L
  • Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Denmark. lcb@life.ku.dk
Koch, T
    Heerkens, T
      Bessonov, K
        Thomsen, P
          Betts, D

            MeSH Terms

            • Animals
            • Bone Marrow Cells / physiology
            • Cells, Cultured
            • Chondrogenesis / physiology
            • Cryopreservation
            • Fetal Blood / cytology
            • Gene Expression Profiling
            • Gene Expression Regulation / physiology
            • Horses / physiology
            • Mesenchymal Stem Cells / cytology
            • Mesenchymal Stem Cells / metabolism

            Citations

            This article has been cited 21 times.
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