Chromatin configuration within the germinal vesicle of horse oocytes: changes post mortem and relationship to meiotic and developmental competence.
Abstract: We evaluated the relationship of initial chromatin configuration to time of oocyte recovery and to nuclear maturation after culture in horse oocytes having compact (Cp) and expanded (Ex) cumuli. In addition, we evaluated the effect of oocyte type, time of recovery, and duration of culture on blastocyst development after intracytoplasmic sperm injection. In oocytes collected within 1 h of slaughter, fibrillar and intermediate chromatin configurations were more prevalent in Cp than in Ex oocytes (68% and 12%, respectively). In Cp oocytes collected after a 5- to 9-h delay, the proportions in the fibrillar and intermediate configurations decreased significantly, and the proportions of degenerating and homogeneously fluorescent configurations increased. When cultured, 20% of oocytes classified as having fibrillar chromatin resumed meiosis, whereas 82% of intermediate and 81% to 86% of condensed chromatin oocytes did so. Meiotic resumption was higher in oocytes recovered immediately after slaughter, but these oocytes took longer to mature. Duration of maturation significantly affected blastocyst development rates in Cp oocytes recovered after a delay (13% and 38% for oocytes matured 24 and 36 h, respectively). Oocytes recovered after a delay had higher blastocyst development rates than did those collected immediately after slaughter. We conclude that the fibrillar and intermediate chromatin configurations may degenerate during ovary storage, resulting in decreased maturation rates, especially of Cp oocytes. Time of oocyte recovery and duration of maturation significantly affect the rate of blastocyst development. Oocytes with Cp and Ex cumuli have similar developmental competence to the blastocyst stage.
Publication Date: 2005-01-12 PubMed ID: 15647456DOI: 10.1095/biolreprod.104.036012Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research explores the relationship between the configuration of chromatin within horse oocytes (egg cells before maturation), the time of oocyte recovery post mortem, and the cell’s ability to mature and develop into a full-grown organism. It was found that different types of chromatin configurations have varying abilities to mature and that the duration of maturation significantly affects the rate of organism development.
Understanding Chromatin Configurations in Oocytes
- The study investigates the variations in chromatin configuration, the complex of DNA and proteins that make up chromosomes, in horse oocytes. These configurations are analyzed within two different types of oocytes, those with compact (Cp) and expanded (Ex) cumuli, structures that provide nourishment to the developing oocyte.
- It is identified that in oocytes collected within an hour of slaughter, fibrillar and intermediate chromatin configurations were more prevalent in Cp oocytes. In contrast, in Cp oocytes collected after a delay of 5-9 hours, the occurrence of these configurations significantly decreased.
- The researchers also studied the effects of the oocyte type, time of recovery, and duration of culture on the development of blastocysts, an early stage of embryonic development, after sperm injection into the oocyte.
Impact on Oocyte Maturation and Development
- The study revealed that oocytes exhibiting fibrillar chromatin had lower meiotic resumption rates, a crucial process for oocyte maturation, compared to intermediate and condensed chromatin oocytes.
- It was also found that immediate recovery after slaughter led to higher meiotic resumption rates, but these oocytes took longer to mature completely.
- The researchers found out that the time taken for oocyte maturation significantly impacts the blastocyst development rate in Cp oocytes, with longer maturation times resulting in higher development rates.
Implications of the Findings
- The study concludes that fibrillar and intermediate chromatin configurations might degenerate during ovary storage, leading to decreased maturation rates, especially in Cp oocytes.
- It also provides an important understanding that the timing of oocyte recovery and the duration of maturation significantly impact blastocyst development rates.
- The study contributes valuable knowledge to the field by demonstrating that oocytes with different cumuli types (Cp and Ex) have similar developmental competence to reach the blastocyst stage, an important observation for potential genetic and reproductive technologies.
Cite This Article
APA
Hinrichs K, Choi YH, Love LB, Varner DD, Love CC, Walckenaer BE.
(2005).
Chromatin configuration within the germinal vesicle of horse oocytes: changes post mortem and relationship to meiotic and developmental competence.
Biol Reprod, 72(5), 1142-1150.
https://doi.org/10.1095/biolreprod.104.036012 Publication
Researcher Affiliations
- Department of Veterinary, College of Veterinary Medicine, Texas A&M University, Texas 77843-4466, USA. khinrichs@cvm.tamu.edu
MeSH Terms
- Animals
- Blastocyst / cytology
- Chromatin / ultrastructure
- Embryonic Development
- Female
- Horses / anatomy & histology
- In Vitro Techniques
- Male
- Meiosis
- Oocytes / cytology
- Oocytes / growth & development
- Oocytes / ultrastructure
- Postmortem Changes
- Sperm Injections, Intracytoplasmic
- Time Factors
Citations
This article has been cited 18 times.- Angel-Velez D, Meese T, Hedia M, Fernandez-Montoro A, De Coster T, Pascottini OB, Van Nieuwerburgh F, Govaere J, Van Soom A, Pavani K, Smits K. Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation. Int J Mol Sci 2023 Apr 7;24(8).
- Zhu X, Zhao S, Xu S, Zhang D, Zhu M, Pan Q, Huang J. Granulosa Cells Improved Mare Oocyte Cytoplasmic Maturation by Providing Collagens. Front Cell Dev Biol 2022;10:914735.
- Martín-Maestro A, Sánchez-Ajofrín I, Maside C, Peris-Frau P, Medina-Chávez DA, Cardoso B, Navarro JC, Fernández-Santos MR, Garde JJ, Soler AJ. Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep. Animals (Basel) 2020 Dec 17;10(12).
- Muller B, Lewis N, Adeniyi T, Leese HJ, Brison DR, Sturmey RG. Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos. Sci Rep 2019 Nov 14;9(1):16778.
- Martinez de Andino EV, Brom-de-Luna JG, Canesin HS, Rader K, Resende HL, Ripley AM, Love CC, Hinrichs K. Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer. J Assist Reprod Genet 2019 Jun;36(6):1237-1250.
- Morohaku K. A way for in vitro/ex vivo egg production in mammals. J Reprod Dev 2019 Aug 9;65(4):281-287.
- Salgado RM, Brom-de-Luna JG, Resende HL, Canesin HS, Hinrichs K. Lower blastocyst quality after conventional vs. Piezo ICSI in the horse reflects delayed sperm component remodeling and oocyte activation. J Assist Reprod Genet 2018 May;35(5):825-840.
- Abdel-Salam Z, Harith MA. Laser researches on livestock semen and oocytes: A brief review. J Adv Res 2015 May;6(3):311-7.
- Martino NA, Dell'Aquila ME, Filioli Uranio M, Rutigliano L, Nicassio M, Lacalandra GM, Hinrichs K. Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential. Reprod Biol Endocrinol 2014 Oct 11;12:99.
- Abdoon AS, Abdel-Rahman HA, Shawki SM, Kandil OM, Fathalla SI. Influence of follicle size, methods of retrieval on oocytes yield and morphology in Egyptian Jennies ovaries with special reference to maturation rate in vitro. Vet Res Commun 2014 Dec;38(4):287-95.
- Zhang GL, Ma JY, Sun Q, Hu MW, Yang XY, Gao SH, Jiang GJ. Effects of postmortem interval on mouse ovary oocyte survival and maturation. PLoS One 2014;9(5):e98384.
- Ambruosi B, Uranio MF, Sardanelli AM, Pocar P, Martino NA, Paternoster MS, Amati F, Dell'Aquila ME. In vitro acute exposure to DEHP affects oocyte meiotic maturation, energy and oxidative stress parameters in a large animal model. PLoS One 2011;6(11):e27452.
- Cremonesi F, Anderson K, Lange-Consiglio A. Efficacy of tuohy needle in oocytes collection from excised mare ovaries. Vet Med Int 2010 Aug 5;2010.
- Lange Consiglio A, Dell'Aquila ME, Fiandanese N, Ambruosi B, Cho YS, Bosi G, Arrighi S, Lacalandra GM, Cremonesi F. Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse. Reprod Biol Endocrinol 2009 Oct 16;7:113.
- Sun X, Li Z, Yi Y, Ding W, Chen J, Engelhardt JF, Leno GH. Chromatin configurations in the ferret germinal vesicle that reflect developmental competence for in vitro maturation. Reprod Domest Anim 2009 Apr;44(2):320-5.
- Gong Z, Wang Y, Tang J, Xu Y, Wang H, Zhang Y, Xiong L, Sun C, Li Y, Yang Y, Yao M, Cai H, Man Z, Xuan S, Tang Y, Zhao Z, Sun J, Liu D, Su Y, Xu X, Luo M, Sui H. Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo. PLoS One 2025;20(2):e0312241.
- Walter J, Colleoni S, Lazzari G, Fortes C, Grossmann J, Roschitzki B, Laczko E, Naegeli H, Bleul U, Galli C. Maturational competence of equine oocytes is associated with alterations in their 'cumulome'. Mol Hum Reprod 2024 Sep 12;30(9).
- Teng M, Zhao M, Mu B, Lei A. Allogenic Follicular Fosterage Technology: Problems, Progress and Potential. Vet Sci 2024 Jun 17;11(6).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
