Classification of Actinobacillus spp isolates from horses involved in mare reproductive loss syndrome.
Abstract: To identify Actinobacillus spp isolates recovered from fetuses and pericardial fluid from horses affected with mare reproductive loss syndrome (MRLS) and determine whether these bacterial species are the same as those isolated from clinically normal horses. Methods: Isolates of actinobacilli recovered from 18 horses with pericarditis and 109 fetuses aborted by mares affected by MRLS. Procedures-Actinobacillus spp isolates were identified to the level of species or subspecies by use of conventional phenotypic tests and biochemical and enzyme test kits. The 16S rRNA gene from selected isolates was amplified, purified, and sequenced. Sequence data were compared with sequence data for actinobacilli in GenBank. Results: Of the 109 isolates obtained from fetuses, 14 were Actinobacillus equuli subsp equuli, 65 were A equuli subsp haemolyticus, 28 were Bisgaard taxon 10-like bacterium, and 2 were Actinobacillus genomospecies 1. Of the 18 isolates from horses with pericarditis, 4 were A equuli subsp equuli, 13 were A equuli subsp haemolyticus, and 1 was Bisgaard taxon 10-like bacterium. Comparisons with published data and GenBank data revealed that the isolates recovered from horses with MRLS were the same as those isolated from the oral cavity or alimentary tract of healthy horses. Conclusions: Actinobacillus spp isolates recovered from fetuses and pericardial fluid samples of horses affected by MRLS in 2001 to 2003 were identical to Actinobacillus spp found in the oral cavity and alimentary tracts of healthy horses.
Publication Date: 2006-08-03 PubMed ID: 16881857DOI: 10.2460/ajvr.67.8.1426Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Diagnosis
- Disease control
- Disease Diagnosis
- Disease Etiology
- Disease Management
- Disease Outbreaks
- Disease Prevention
- Disease Surveillance
- Disease Transmission
- Disease Treatment
- Equine Diseases
- Equine Health
- Equine Research
- Horses
- Infection
- Infectious Disease
- Mare's Milk
- Veterinary Care
- Veterinary Medicine
- Veterinary Research
Summary
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The research article focuses on identifying the Actinobacillus spp isolates found in fetuses and pericardial fluid from horses affected by mare reproductive loss syndrome (MRLS). The findings reveal that these isolates are identical to the bacteria species found in the oral cavity and alimentary tracts of healthy horses.
Methodology
- The article outlines that the bacteria investigated in this research are Actinobacillus spp, which were isolated from 18 horses with pericarditis and from 109 fetuses which were aborted by mares affected by MRLS.
- These isolates were then identified down to species or subspecies level. This was done using conventional phenotypic tests and a variety of biochemical and enzyme test kits.
- Furthermore, the 16S rRNA gene from chosen isolates was amplified, cleaned up, then sequenced.
- The data from this sequencing were compared with similar data from the GenBank database, which contains a wide range of information on many strains of bacteria, including actinobacilli.
Results
- The results section of the article indicates that out of the 109 isolates obtained from fetuses, 14 were Actinobacillus equuli subsp equuli, 65 were A equuli subsp haemolyticus, 28 were Bisgaard taxon 10-like bacterium, and 2 were Actinobacillus genomospecies 1.
- From the 18 isolates from horses with pericarditis, 4 were A equuli subsp equuli, 13 were A equuli subsp haemolyticus, and 1 was Bisgaard taxon 10-like bacterium.
- These results were then compared with other research data and prior GenBank data.
Conclusion
- In conclusion, the research determined that the Actinobacillus spp isolated from horses affected by MRLS are the same as those found in the oral cavity or alimentary tracts of healthy horses.
- This suggests that MRLS may not be exclusively caused by a novel pathogen but potentially by a bacteria already present in healthy horses under certain conditions.
Cite This Article
APA
Donahue JM, Sells SF, Bolin DC.
(2006).
Classification of Actinobacillus spp isolates from horses involved in mare reproductive loss syndrome.
Am J Vet Res, 67(8), 1426-1432.
https://doi.org/10.2460/ajvr.67.8.1426 Publication
Researcher Affiliations
- Livestock Disease Diagnostic Center, College of Agriculture, University of Kentucky, Lexington, 40511-4125, USA.
MeSH Terms
- Aborted Fetus / microbiology
- Abortion, Veterinary / microbiology
- Actinobacillus / classification
- Actinobacillus / genetics
- Animals
- Female
- Horse Diseases / microbiology
- Horses
- Pericardial Effusion / microbiology
- RNA, Ribosomal, 16S / genetics
Citations
This article has been cited 5 times.- Vereecke N, Vandekerckhove A, Theuns S, Haesebrouck F, Boyen F. Whole genome sequencing to study antimicrobial resistance and RTX virulence genes in equine Actinobacillus isolates.. Vet Res 2023 Apr 5;54(1):33.
- Kamali M, Carossino M, Del Piero F, Peak L, Mitchell MS, Willette J, Baker R, Li F, Kenéz Á, Balasuriya UBR, Go YY. Pathological Features and Genomic Characterization of an Actinobacillus equuli subsp. equuli Bearing Unique Virulence-Associated Genes from an Adult Horse with Pleuropneumonia.. Pathogens 2023 Jan 31;12(2).
- Chapuis RJJ, Ragno VM, Ariza CA, Movasseghi AR, Sayi S, Uehlinger FD, Montgomery JB. Septic fibrinous pericarditis in 4 horses in Saskatchewan following an outbreak of forest tent caterpillars in 2017.. Can Vet J 2020 Jul;61(7):724-730.
- Volpato G, Di Nardo A, Rossi D, Saleh SM, Broglia A. 'Everybody knows', but the rest of the world: the case of a caterpillar-borne reproductive loss syndrome in dromedary camels observed by Sahrawi pastoralists of Western Sahara.. J Ethnobiol Ethnomed 2013 Jan 10;9:5.
- Oz HS, Ebersole JL, de Villiers WJ. The macrophage pattern recognition scavenger receptors SR-A and CD36 protect against microbial induced pregnancy loss.. Inflamm Res 2011 Jan;60(1):93-7.
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