Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues.
Abstract: Claudins, which are known as transmembrane proteins play an essential role in tight junctions (TJs) to form physical barriers and regulate paracellular transportation. To understand equine diseases, it is helpful to measure the tissue-specific expression of TJs in horses. Major equine diseases such as colic and West Nile cause damage to TJs. In this study, the expression level and distribution of claudin-1, -2, -4, and -5 in eight tissues were assessed by Western blotting and immunohistochemistry methods. Claudin-1 was primarily identified in the lung, duodenum, and uterus, claudin-2 was evenly observed in equine tissues, claudin-4 was abundantly detected in the liver, kidney and uterus, and claudin-5 was strongly expressed in the lung, duodenum, ovary, and uterus, as determined by Western blotting method. The localization of equine claudins was observed by immunohistochemistry methods. These findings provide knowledge regarding the expression patterns and localization of equine claudins, as well as valuable information to understand tight junction-related diseases according to tissue specificity and function of claudins in horses.
Publication Date: 2016-04-01 PubMed ID: 27030194PubMed Central: PMC5204021DOI: 10.4142/jvs.2016.17.4.445Google Scholar: Lookup
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- Journal Article
Summary
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The research focuses on understanding the role and distribution of certain proteins called claudins in the tissues of horses. These proteins play a significant role in maintaining the health and functioning of cells and have implications in understanding diseases such as colic and West Nile.
The Role of Claudins
- Claudins are transmembrane proteins that form an essential component of tight junctions (TJs), which are part of the cells that form various tissues and organs in the body.
- These proteins not only help form physical barriers but also regulate the transfer of substances between cells, a process known as paracellular transportation.
- Knowing the distribution and levels of these proteins in horse tissues could provide valuable insights into how diseases that affect TJs, such as colic and West Nile, occur and progress.
The Study and its Methods
- The study focused on the expression and distribution of four types of claudins – claudin-1, claudin-2, claudin-4, and claudin-5 – in eight different types of tissues.
- The levels and distribution of these proteins were assessed using two methods: Western blotting, which is a technique used to detect specific proteins in a sample, and immunohistochemistry, which involves staining proteins to visualise their location in tissues.
Findings of the Study
- The study found that the distribution of these claudins varied across different tissues. Claudin-1 was primarily identified in the lung, duodenum, and uterus, claudin-2 was evenly observed in equine tissues, claudin-4 in the liver, kidney and uterus, and claudin-5 was most expressed in the lung, duodenum, ovary, and uterus.
- The localization of these claudins in horse tissues was also visualized using immunohistochemistry.
Importance of Findings
- The findings provide new knowledge on where these claudins are most expressed in horse tissues, information that was previously unknown.
- This information is critical to understanding the function of these proteins and how they may be implicated in TJ-related diseases in horses.
Cite This Article
APA
Lee B, Kang HY, Lee DO, Ahn C, Jeung EB.
(2016).
Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues.
J Vet Sci, 17(4), 445-451.
https://doi.org/10.4142/jvs.2016.17.4.445 Publication
Researcher Affiliations
- Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea.
- Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea.
- Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea.
- Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea.
- Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea.
MeSH Terms
- Animals
- Blotting, Western / veterinary
- Claudins / genetics
- Claudins / metabolism
- Female
- Gene Expression Regulation
- Horses / genetics
- Horses / metabolism
- Immunohistochemistry / veterinary
- Male
- Tissue Distribution
Conflict of Interest Statement
There is no conflict of interest.
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This article includes 28 references
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Citations
This article has been cited 4 times.- Kozieł MJ, Kowalska K, Piastowska-Ciesielska AW. Claudins: New Players in Human Fertility and Reproductive System Cancers. Cancers (Basel) 2020 Mar 18;12(3).
- Ahmad R, Rah B, Bastola D, Dhawan P, Singh AB. Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching. Sci Rep 2017 Jul 11;7(1):5125.
- Ahmad R, Sorrell MF, Batra SK, Dhawan P, Singh AB. Gut permeability and mucosal inflammation: bad, good or context dependent. Mucosal Immunol 2017 Mar;10(2):307-317.
- Kshersagar J, Damle MN, Sharma R, Joshi MG. Cell Communication in Endometrium: Understanding and Improving Endometrial Biomarkers. Adv Exp Med Biol 2025;1499:27-50.
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