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Home / Equine Research Bank / J S Afr Vet Assoc / Clinical findings, synovial fluid cytology and growth factor...
Journal of the South African Veterinary Association2019; 90; e1-e9; doi: 10.4102/jsava.v90i0.1721

Clinical findings, synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis.

Abstract: Osteoarthritis is a common cause of lameness in horses, resulting in poor performance. Intra-articular platelet-rich plasma can deliver a collection of bioactive molecules, such as autologous growth factors and proteins involved in the quality of tissue repair. Horses (n=5) with osteoarthritis affecting antebrachiocarpal, middle carpal or metacarpophalangeal joints, and horses (n=5) without osteoarthritis of the corresponding joints (radiographically free of osteoarthritis), were used for the production of platelet-rich plasma which was subsequently injected into selected joints. Clinical and synovial fluid changes after intra-articular injection of platelet-rich plasma as well as synovial platelet-derived growth factor-BB and transforming growth factor-beta 1 concentration changes were evaluated in these joints and compared between normal joints and joints with osteoarthritis. A gravity filtration system produced a moderately concentrated platelet-rich plasma, representing a 4.7-fold increase in baseline platelet concentration. The synovial effusion score was significantly different between the control joints and joints with osteoarthritis on Day 0 with a higher score in the group with osteoarthritis. Within the control group, the synovial effusion score was significantly higher on Days 1 and 2 compared to Day 0. For both groups, the synovial fluid nucleated cell count, predominantly intact neutrophils, was significantly increased on Days 1 and 2, with no significant difference between groups. The mean synovial platelet-derived growth factor-BB and transforming growth factor-beta 1 concentrations were increased for both groups but significantly lowered in the group with osteoarthritis on Day 1 compared to normal joints. Concentrations for platelet-derived growth factor-BB remained unchanged on Day 5, compared to Day 1, with no significant difference between groups. In conclusion, intra-articular treatment with platelet-rich plasma resulted in increased synovial growth factor concentrations in joints but with lower concentrations in joints with osteoarthritis. A transient inflammatory reaction was seen both clinically as an increase in synovial effusion and cytologically in both normal joints and joints with osteoarthritis.
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Publication Date: 2019-05-23 PubMed ID: 31170778PubMed Central: PMC6556911DOI: 10.4102/jsava.v90i0.1721Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study probes the effects of delivering platelet-rich plasma (PRP) intra-articularly in horses diagnosed with osteoarthritis, and compares the results to those in horses without the mentioned ailment. Results indicate an elevation in synovial growth factor concentrations following treatment with PRP, however, those with osteoarthritis showed lower concentrations.

Objective and Method

  • The objective of this study was to understand the influence of injecting platelet-rich plasma (PRP) into the joints of horses suffering from osteoarthritis. The method applied compared the results with a control group: horses without osteoarthritis.
  • In order to achieve this objective, PRP was sourced from affected horses and from the control group. The plasma was then injected back into specific joints of the donor horse, allowing the researchers to study the resulting changes in clinical and synovial fluid dynamics.

Key Findings

  • The findings of the study established that using a gravity filtration system yielded a moderate concentration of PRP, having a 4.7 times increase over the initial platelet concentration.
  • On Day 0 of the study, the synovial effusion score was high for horses with osteoarthritis compared to the control group. However, for the control group, this score elevated post the injection on Day 1 and 2, in comparison to Day 0.
  • The cell count of synovial fluid, mainly intact neutrophils, shot up on Day 1 and 2 for horses without exception, but without any significant difference between the two groups.
  • Two factors, the platelet-derived growth factor-BB and the transforming growth factor-beta 1, escalated in concentration for both groups, noticeably lower for those with osteoarthritis on Day 1.
  • By Day 5, the concentration of platelet-derived growth factor-BB showed no significant change compared to Day 1 without revealing difference among the two groups.

Conclusion

  • The application of PRP intra-articularly led to the rise of synovial growth factor concentrations in joints. However, the concentrations were found to be relatively lower in horses that had osteoarthritis.
  • Another point that surfaced was that a short-term inflammatory reaction – reflected in amplified synovial effusion and cytologic counts – was noted in both the control group and the horses diagnosed with osteoarthritis.

Cite This Article

APA
Smit Y, Marais HJ, Thompson PN, Mahne AT, Goddard A. (2019). Clinical findings, synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis. J S Afr Vet Assoc, 90, e1-e9. https://doi.org/10.4102/jsava.v90i0.1721

Publication

Journal of the South African Veterinary Association
ISSN: 2224-9435
NlmUniqueID: 7503122
Country: South Africa
Language: English
Volume: 90
Pages: e1-e9

Researcher Affiliations

Smit, Yolandi
  • Department of Companion Animal Clinical Studies, University of Pretoria, Onderstepoort. yolandi.smit@up.ac.za.
Marais, Hendrik J
    Thompson, Peter N
      Mahne, Arnold T
        Goddard, Amelia

          MeSH Terms

          • Animals
          • Female
          • Horse Diseases / diagnostic imaging
          • Horse Diseases / drug therapy
          • Horses
          • Injections, Intra-Articular / veterinary
          • Intercellular Signaling Peptides and Proteins / analysis
          • Lameness, Animal / complications
          • Lameness, Animal / diagnostic imaging
          • Male
          • Osteoarthritis / complications
          • Osteoarthritis / drug therapy
          • Osteoarthritis / veterinary
          • Platelet-Rich Plasma
          • Synovial Fluid / cytology
          • Treatment Outcome

          Conflict of Interest Statement

          The authors declare no other financial or personal conflicts of interest that may have inappropriately influenced them in writing this article. No employees or consultants have benefitted financially from Pall Corporation or its products.

          References

          This article includes 58 references
          1. Abellanet I, Prades M. Intra-articular platelet rich plasma (PRP) therapy: Evaluation in 42 sport horses with OA. 11th International Congress of World Equine Veterinary Association proceedings, Guarujá, SP, Brazil, September 24–27, 2009.
          2. Albro MB, Cigan AD, Nims RJ, Yeroushalmi KJ, Oungoulian SR, Hung CT. Shearing of synovial fluid activates latent TGF-B. Osteoarthritis and Cartilage 20, 1374–1382, 2012.
            doi: 10.1016/j.joca.2012.07.006pmc: PMC3448789pubmed: 22858668google scholar: lookup
          3. Alcaraz MJ, Megías J, García-Arnandis I, Clérigues V, Guillén MI. New molecular targets for the treatment of osteoarthritis. Biochemical Pharmacology 80, 13–21, 2010.
            doi: 10.1016/j.bcp.2010.02.017pubmed: 20206140google scholar: lookup
          4. Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M. Growth factors and cytokines in wound healing. Wound Repair and Regeneration 16, 585–601, 2008.
            doi: 10.1111/j.1524-475X.2008.00410.xpubmed: 19128254google scholar: lookup
          5. Carmona JU, Argüelles D, Climent F, Prades M. Autologous platelet concentrates as a treatment of horses with osteoarthritis: A preliminary pilot clinical study. Journal of Equine Veterinary Science 27, 167–170, 2007.
            doi: 10.1016/j.jevs.2007.02.007google scholar: lookup
          6. Carmona JU, Ríos DL, López C, Álvarez ME, Pérez JE, Bohórquez ME. In vitro effects of platelet-rich gel supernatants on histology and chondrocyte apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide. BMC Veterinary Research 12, 135, 2016.
            doi: 10.1186/s12917-016-0759-8pmc: PMC4929746pubmed: 27369779google scholar: lookup
          7. Castelijns G, Crawford A, Schaffer J, Ortolano GA, Beauregard T, Smith RK. Evaluation of a filter-prepared platelet concentrate for the treatment of suspensory branch injuries in horses. Veterinary and Comparative Orthopaedics and Traumatology 24, 363–369, 2011.
            doi: 10.3415/VCOT-11-01-0001pubmed: 21887455google scholar: lookup
          8. Castillo TN, Pouliot MA, Kim HJ, Dragoo JL. Comparison of growth factor and platelet concentration from commercial platelet-rich plasma separation systems. American Journal of Sports Medicine 39, 266–272, 2011.
            doi: 10.1177/0363546510387517pubmed: 21051428google scholar: lookup
          9. Chen YQ, Chou PL, Cheng CY, Chiang CC, Wei MT, Chuang CT. Microrheology of human synovial fluid of arthritis patients studied by diffusing wave spectroscopy. Journal of Biophotonics 5, 777–784, 2012.
            doi: 10.1002/jbio.201100128pubmed: 22415866google scholar: lookup
          10. Civinini R, Nistri L, Martini C, Redl B, Ristori G, Innocenti M. Growth factors in the treatment of early osteoarthritis. Clinical Cases Mineral Bone Metabolism 10, 26–29, 2013.
            doi: 10.11138/ccmbm/2013.10.1.026pmc: PMC3710006pubmed: 23858307google scholar: lookup
          11. Conrozier T, Mathieu P, Vignon E, Piperno M, Rinaudo M. Differences in the osteoarthritic synovial fluid composition and rheology between patients with or without flare: A pilot study. Clinical and Experimental Rheumatology 30, 729–734, 2012.
            doi: 10.1016/j.joca.2012.02.404pubmed: 22935334google scholar: lookup
          12. Dohan Ehrenfest MD, Bielecki T, Jimbo R, Barbe G, Del Corso M, Inchingolo F. Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a pure platelet-rich plasma (P-PRP) gel and a leukocyte- and platelet-rich fibrin (L-PRF). Current Pharmaceutical Biotechnology 13, 1145–1152, 2012.
            doi: 10.2174/138920112800624382pubmed: 21740377google scholar: lookup
          13. Filardo G, Kon E, Buda R, Timoncini A, Di Martino A, Cenacchi A. Platelet-rich plasma intra-articular knee injections for the treatment of degenerative cartilage lesions and osteoarthritis. Knee Surgery Sports Traumatology Arthroscopy 19, 528–535, 2011.
            doi: 10.1007/s00167-010-1238-6pubmed: 20740273google scholar: lookup
          14. Fortier LA. Current concepts in joint therapy. 11th International Congress of World Equine Veterinary Association proceedings, Guarujá, SP, Brazil, September 24–27, 2009.
          15. Fortier LA, Baker JU, Strauss EJ, McCarrel TM, Coly BJ. The role of growth factors in cartilage repair. Clinical Orthopaedics and Related Research 469, 2706–2715, 2011.
            doi: 10.1007/s11999-011-1857-3pmc: PMC3171543pubmed: 21403984google scholar: lookup
          16. Frisbie DD, Kawcak CE, Werpy NM, Park RD, Mcllwraith CW. Clinical, biochemical, and histological effects of intra-articular administration of autologous conditioned serum in horses with experimentally induced osteoarthritis. American Journal of Veterinary Research 68, 290–296, 2007.
            doi: 10.2460/ajvr.68.3.290pubmed: 17331019google scholar: lookup
          17. Giraldo CE, Álvarez ME, Carmona JU. Influence of calcium salts and bovine thrombin on growth factor release from equine platelet-rich gel supernatants. Veterinary and Comparative Orthopaedics and Traumatology 30, 1–7, 2017.
            doi: 10.3415/VCOT-16-02-0026pubmed: 27849108google scholar: lookup
          18. Guadilla J, Fiz N, Andia I, Sanchez M. Arthroscopic management and platelet-rich plasma therapy for avascular necrosis of the hip. Knee Surgery Sports Traumatology Arthroscopy 20, 393–398, 2011.
            doi: 10.1007/s00167-011-1587-9pubmed: 21695463google scholar: lookup
          19. Holzer N, Salvo D, Marijnissen ACA, Vincken KL, Ahmad AC, Serra E. Radiographic evaluation of posttraumatic osteoarthritis of the ankle: The Kellgren-Lawrence scale is reliable and correlates with clinical symptoms. Osteoarthritis and Cartilage 23, 363–369, 2015.
            doi: 10.1016/j.joca.2014.11.010pubmed: 25463444google scholar: lookup
          20. Iqbal J, Dudhia J, Bird JLE, Bayliss M. Age-related effects of TGF-β on proteoglycan synthesis in equine articular cartilage. Biochemical and Biophysical Research Communications 274, 467–471, 2000.
            doi: 10.1006/bbrc.2000.3167pubmed: 10913361google scholar: lookup
          21. Ishida K, Kuroda R, Miwa M, Tabata Y, Hokugo A, Kawamoto T. The regenerative effects of platelet-rich plasma on meniscal cells in vitro and it’s in vivo application with biodegradable gelatin hydrogel. Journal of Tissue Engineering 13, 1103–1112, 2007.
            doi: 10.1089/ten.2006.0193pubmed: 17348798google scholar: lookup
          22. Jacobsen S, Kjelgaard-Hansen M, Hagbard Petersen H, Jensen AL. Evaluation of a commercially available human serum amyloid A (SAA) turbidometric immunoassay for determination of equine SAA concentrations. The Veterinary Journal 172, 315–319, 2006.
            doi: 10.1016/j.tvjl.2005.04.021pubmed: 15950503google scholar: lookup
          23. Jacobsen S, Thomsen MH, Nanni S. Concentrations of serum amyloid A in serum and synovial fluid from healthy horses and horses with joint disease. American Journal of Veterinary Research 67, 1738–1742, 2006.
            doi: 10.2460/ajvr.67.10.1738pubmed: 17014325google scholar: lookup
          24. Judy CE, Galuppo LD. Evaluation of iatrogenic hemarthrosis of the metacarpophalangeal joint as a method of induction of temporary reversible lameness in horses. American Journal of Veterinary Research 66, 1084–1089, 2005.
            doi: 10.2460/ajvr.2005.66.1084pubmed: 16008235google scholar: lookup
          25. Kon E, Mandelbaum B, Buda R, Filardo G, Delcogliano M, Timoncini A. Platelet-rich plasma intra-articular injection versus hyaluronic acid viscosupplementation as treatments for cartilage pathology: From early degeneration to osteoarthritis. Arthroscopy 27, 1490–1501, 2011.
            doi: 10.1016/j.arthro.2011.05.011pubmed: 21831567google scholar: lookup
          26. Liu J, Yuan T, Zhang C. Effects of platelet-rich plasma on synovitis of rabbit knee. Zhongguo Xxiu Fu Chong Jian Wai Ke Za Zhi 25, 285–290, 2011.
            pubmed: 21500578
          27. May SA, Hooke RE, Lees P. Equine chondrocytes activation by a variety of stimuli. British Veterinary Journal 148, 389–397, 1992.
            doi: 10.1016/0007-1935(92)90026-Wpubmed: 1422781google scholar: lookup
          28. Mazzucco L, Balbo V, Cattana E, Guaschino R, Borzini P. Not every PRP-gel is born equal. Evaluation of growth factor availability for tissues through four PRP-gel preparations: Fibrinet, RegenPRP-Kit, Plateltex and one manual procedure. Vox Sang 97, 110–118, 2009.
            doi: 10.1111/j.1423-0410.2009.01188.xpubmed: 19392780google scholar: lookup
          29. Mcllwraith CW. Traumatic arthritis and posttraumatic osteoarthritis in the horse. In Mcllwraith CW, Frisbie DD, Kawcak CE & van Weeren PR (eds.), Joint disease in the horse, pp. 33–48, Elsevier, MI, 2016.
            doi: 10.1016/B978-1-4557-5969-9.00003-6google scholar: lookup
          30. Mcllwraith CW, Frisbie DD, Kawcak CE. The horse as a model of naturally occurring osteoarthritis. Bone & Joint Research 1, 297–309, 2012.
            doi: 10.1302/2046-3758.111.2000132pmc: PMC3626203pubmed: 23610661google scholar: lookup
          31. Mcllwraith CW, Frisbie DD, Rodkey WG, Kisiday JD, Werpy NM, Kawcak CE. Evaluation of intra-articular mesenchymal stem cells to augment healing of microfractured chondral defects. Arthroscopy 27, 1552–1561, 2011.
            doi: 10.1016/j.arthro.2011.06.002pubmed: 21862278google scholar: lookup
          32. Mei-Dan O, Carmont MR, Laver L, Mann G, Maffulli N, Nyska M. Platelet-rich plasma or hyaluronate in the management of osteochondral lesions of the talus. American Journal of Sports Medicine 40, 534–541, 2012.
            doi: 10.1177/0363546511431238pubmed: 22253252google scholar: lookup
          33. Monteiro SO, Bettencourt EV, Lepage OM. Biologic strategies for intra-articular treatment and cartilage repair. Journal of Equine Veterinary Science 35, 175–190, 2015.
            doi: 10.1016/j.jevs.2015.01.006google scholar: lookup
          34. Nganvongpanit K, Tanvisut S, Yano T, Kongtawelert P. Effect of swimming on clinical functional parameters and serum biomarkers in healthy and osteoarthritic dogs. ISRN Veterinary Science 2014, Article ID 459809.
            pmc: PMC4060742pubmed: 24977044
          35. Nixon AJ, Brower-Toland BD, Sandell LJ. Molecular cloning of equine transforming growth factor-beta 1 reveals equine specific amino acid substitutions in mature peptide sequence. Journal of Molecular Endocrinology 24, 261–272, 2000.
            doi: 10.1677/jme.0.0240261pubmed: 10750027google scholar: lookup
          36. Ovlisen K, Kristensen AT, Jensen AL, Tranholm M. IL-1 beta, IL-6, KC and MCP-1 are elevated in synovial fluid from haemophilic mice with experimentally induced haemarthrosis. Haemophilia 15, 802–810, 2009.
            doi: 10.1111/j.1365-2516.2008.01973.xpubmed: 19444976google scholar: lookup
          37. Pigott JH, Ishihara A, Wellman ML, Russell DS, Bertone AL. Inflammatory effects of autologous, genetically modified autologous, allogeneic, and xenogeneic mesenchymal stem cells after intra-articular injection in horses. Veterinary and Comparative Orthopaedics and Traumatology 26, 453–460, 2013.
            doi: 10.3415/VCOT-13-01-0008pubmed: 24080668google scholar: lookup
          38. Ríos DL, López C, Álvarez ME, Samudio IJ, Caromona JU. Effects over time of two platelet gel supernatants on growth factor, cytokine and hyaluronan concentrations in normal synovial membrane explants challenged with lipopolysaccharide. BMC Musculoskeletal Disorders 16, 153, 2015.
            doi: 10.1186/s12891-015-0605-3pmc: PMC4475292pubmed: 26092588google scholar: lookup
          39. Ríos DL, López C, Carmona JU. Platelet-rich gel supernatants stimulate the release of anti-inflammatory proteins on culture media of normal equine synovial membrane explants. Veterinary Medicine International Hindawi Publishing Corporation, London, United Kingdom, 2015.
            pmc: PMC4451761pubmed: 26090267
          40. Roh YH, Kim W, Park KU, Oh JH. Cytokine-release kinetics of platelet-rich plasma according to various activation protocols. Bone & Joint Research 5, 37–45, 2016.
            doi: 10.1302/2046-3758.52.2000540pmc: PMC4852788pubmed: 26862077google scholar: lookup
          41. Ross MW. Movement. In Ross MW & Dyson SJ (eds.), Diagnosis and management of lameness in the horse, pp. 64–80, Ross, St. Louis, MO, 2011.
          42. Saito M, Takahashi KA, Arai Y, Inoue A, Sakao K, Tonomura H. Intra-articular administration of platelet-rich plasma with biodegradable gelatin hydrogel microsphere prevents osteoarthritis progression in the rabbit knee. Clinical and Experimental Rheumatology 27, 201–207, 2009.
            pubmed: 19473558
          43. Sampson S, Reed M, Silvers H, Meng M, Mandelbaum B. Injection of platelet-rich plasma in patients with primary and secondary knee osteoarthritis: A pilot study. American Journal of Physical Medicine & Rehabilitation 89, 961–969, 2010.
            doi: 10.1097/PHM.0b013e3181fc7edfpubmed: 21403592google scholar: lookup
          44. Sanchez M, Anitua E, Azofra J, Aguirre JJ, Andia I. Intra-articular injection of an autologous preparation rich in growth factors for the treatment of knee OA: A retrospective cohort study. Clinical and Experimental Rheumatology 26, 910–913, 2008.
            pubmed: 19032827
          45. Sanchez Teran AF, Rubio-Martinez LM, Villarino NF, Sanz MG. Effects of repeated intra-articular administration of amikacin on serum amyloid A, total protein and nucleated cell count in synovial fluid from healthy horses. Equine Veterinary Journal 44, 12–16, 2012.
            doi: 10.1111/j.2042-3306.2012.00637.xpubmed: 23447871google scholar: lookup
          46. Sheth U, Simunovic N, Klein G, Fu F, Einhorn TA, Schemitsch E. Efficacy of autologous platelet-rich plasma use for orthopaedic indications: A meta-analysis. Journal of Bone & Joint Surgery 94, 298–307, 2012.
            doi: 10.2106/JBJS.K.00154pubmed: 22241606google scholar: lookup
          47. Steel CM. Equine synovial fluid analysis. Veterinary Clinics of North America. Equine Practice 24, 437–454, 2008.
            doi: 10.1016/j.cveq.2008.05.004pubmed: 18652964google scholar: lookup
          48. Sun Y, Feng Y, Zhang CQ, Chen SB, Cheng XG. The regenerative effect of platelet-rich plasma on healing in large osteochondral defects. International Orthopaedics 34, 589–597, 2010.
            doi: 10.1007/s00264-009-0793-2pmc: PMC2903145pubmed: 19434411google scholar: lookup
          49. Sutton S, Clutterbuck A, Harris P, Gent T, Freeman S, Foster N. The contribution of the synovium, synovial derived inflammatory cytokines and neuropeptides to the pathogenesis of osteoarthritis. The Veterinary Journal 179, 10–24, 2009.
            doi: 10.1016/j.tvjl.2007.08.013pubmed: 17911037google scholar: lookup
          50. Textor JA, Tablin F. Intra-articular use of platelet-rich product in normal horses: Clinical signs and cytological responses. Veterinary Surgery 42, 499–510, 2013.
            doi: 10.1111/j.1532-950X.2013.12015.xpubmed: 23631631google scholar: lookup
          51. Textor JA, Willits N, Tablin F. Synovial fluid growth factor and cytokine concentrations after intra-articular injection of a platelet-rich product in horses. The Veterinary Journal 198, 217–223, 2013.
            doi: 10.1016/j.tvjl.2013.07.020pubmed: 23992870google scholar: lookup
          52. Van Den Berg WB, Van Der Kraan PM, Scharstuhl A, Van Beuningen HM. Growth factors and cartilage repair. Clinical Orthopaedics and Related Research 391, 244–250, 2001.
            doi: 10.1097/00003086-200110001-00023pubmed: 11603708google scholar: lookup
          53. Van Der Kraan PM. The changing role of TGF-β in healthy, ageing and osteoarthritic joints. Nature Reviews Rheumatology 13, 155–163, 2017.
            doi: 10.1038/nrrheum.2016.219pubmed: 28148919google scholar: lookup
          54. Wei LC, Lei GH, Sheng PY, Gao SG, Xu M, Jiang W. Efficacy of platelet-rich plasma combined with allograft bone in the management of displaced intra-articular calcaneal fractures: A prospective cohort study. Journal of Orthopaedic Research 10, 1570–1576, 2012.
            doi: 10.1002/jor.22118pubmed: 22488880google scholar: lookup
          55. Wei X, Messner K. Age- and injury-dependent concentrations of transforming growth factor-β1 and proteoglycan fragments in rabbit knee joint fluid. Osteoarthritis Cartilage 6, 10–18, 1998.
            doi: 10.1053/joca.1997.0087pubmed: 9616434google scholar: lookup
          56. Xie X, Zhang C, Tuan R. Biology of platelets-rich plasma and its clinical application in cartilage repair. Arthritis Research & Therapy 16, 204, 2014.
            doi: 10.1186/ar4493pmc: PMC3978832pubmed: 25164150google scholar: lookup
          57. Yin W, Shanmugavelayudam SK, Rubenstein DA. The effect of physiologically relevant dynamic shear stress on platelet and endothelial cell activation. Thrombosis Research 127, 235–241, 2011.
            doi: 10.1016/j.thromres.2010.11.021pubmed: 21172720google scholar: lookup
          58. Zimmerman R, Jakubietz R, Jakubietz M. Different preparation methods to obtain components as a source of growth factors for local applications. Transfusion 41, 1217–1224, 2001.
            doi: 10.1046/j.1537-2995.2001.41101217pubmed: 11606819google scholar: lookup

          Citations

          This article has been cited 11 times.
          1. Carmona JU, López C, Argüelles D. Addressing Heterogeneity in Equine PRP Therapies: A Scoping Review of Methods, Evidence, and Commercial Validation. Animals (Basel) 2025 Dec 13;15(24).
            doi: 10.3390/ani15243586pubmed: 41463871google scholar: lookup
          2. Bungărdean D, Pall E, Daradics Z, Popescu M, Tripon MA, Lupșan AF, Crecan CM, Morar IA, Nicolescu A, Bora FD, Marcus I. In Vitro Effects of PRP, Ozonized PRP, Hyaluronic Acid, Paracetamol, and Polyacrylamide on Equine Synovial Fluid-Derived Mesenchymal Stem Cells. Life (Basel) 2025 Oct 4;15(10).
            doi: 10.3390/life15101558pubmed: 41157231google scholar: lookup
          3. Carmona JU, López C. Platelet-Rich Plasma in Equine Osteoarthritis: A Systematic Review of Clinical and Experimental Evidence. Animals (Basel) 2025 Sep 9;15(18).
            doi: 10.3390/ani15182647pubmed: 41007891google scholar: lookup
          4. Kooy S, Constant J, Cole R, Boone L. Safety study of leucoreduced allogeneic pooled freeze-dried platelet-rich plasma in healthy equine joints. Front Vet Sci 2025;12:1625431.
            doi: 10.3389/fvets.2025.1625431pubmed: 40727274google scholar: lookup
          5. Bernardini C, Romagnoli N, Casalini I, Turba ME, Spadari A, Forni M, Gentilini F. Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate. Int J Vet Sci Med 2024;12(1):71-80.
            doi: 10.1080/23144599.2024.2380586pubmed: 39119550google scholar: lookup
          6. Mayet A, Zablotski Y, Roth SP, Brehm W, Troillet A. Systematic review and meta-analysis of positive long-term effects after intra-articular administration of orthobiologic therapeutics in horses with naturally occurring osteoarthritis. Front Vet Sci 2023;10:1125695.
            doi: 10.3389/fvets.2023.1125695pubmed: 36908512google scholar: lookup
          7. Jacobsen S, Mortensen CD, Høj EA, Vinther AM, Berg LC, Adler DMT, Verwilghen D, van Galen G. Neutrophil Gelatinase-Associated Lipocalin in Synovial Fluid from Horses with and without Septic Arthritis. Animals (Basel) 2022 Dec 21;13(1).
            doi: 10.3390/ani13010029pubmed: 36611638google scholar: lookup
          8. Maldonado MD, Parkinson SD, Story MR, Haussler KK. The Effect of Chiropractic Treatment on Limb Lameness and Concurrent Axial Skeleton Pain and Dysfunction in Horses. Animals (Basel) 2022 Oct 19;12(20).
            doi: 10.3390/ani12202845pubmed: 36290230google scholar: lookup
          9. Garbin LC, Contino EK, Olver CS, Frisbie DD. A safety evaluation of allogeneic freeze-dried platelet-rich plasma or conditioned serum compared to autologous frozen products equivalents in equine healthy joints. BMC Vet Res 2022 Apr 18;18(1):141.
            doi: 10.1186/s12917-022-03225-4pubmed: 35436878google scholar: lookup
          10. Ragab GH, Halfaya FM, Ahmed OM, Abou El-Kheir W, Mahdi EA, Ali TM, Almehmadi MM, Hagag U. Platelet-Rich Plasma Ameliorates Monosodium Iodoacetate-Induced Ankle Osteoarthritis in the Rat Model via Suppression of Inflammation and Oxidative Stress. Evid Based Complement Alternat Med 2021;2021:6692432.
            doi: 10.1155/2021/6692432pubmed: 33531920google scholar: lookup
          11. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals (Basel) 2021 Jan 19;11(1).
            doi: 10.3390/ani11010234pubmed: 33477808google scholar: lookup
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