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Frontiers in microbiology2019; 10; 2288; doi: 10.3389/fmicb.2019.02288

Co-resistance to Amoxicillin and Tetracycline as an Indicator of Multidrug Resistance in Escherichia coli Isolates From Animals.

Abstract: To examine the relevance of co-resistance to amoxicillin and tetracycline as an indicator of multidrug resistance (MDR) in animal health. Methods: isolates collected between 2012 and 2016 by the French surveillance network for antimicrobial resistance in diseased animals (RESAPATH) were analyzed. The proportions of MDR isolates and the proportions of isolates presenting co-resistance to amoxicillin and tetracycline were calculated for seven animal species (cattle, horse, dog, swine, poultry, duck, and turkey). The degree of agreement between these two proportions was estimated by calculating the kappa value. Results: In total, 55,904 isolates were analyzed. MDR proportions were variable among animal species, ranging from 21.9% [20.2; 23.7] in horses to 56.0% [55.4; 56.7] in cattle. A similar situation was observed for proportions of isolates with co-resistance to amoxicillin and tetracycline, with the highest value for cattle 65.0% [64.3; 65.6]. This co-resistance was also most often associated with resistance to other antibiotics, regardless of the animal species considered. Comparative analysis showed substantial agreement between MDR and this co-resistance, with a kappa value of 0.75, all animal species considered. Conclusions: Given the widespread use of penicillins and tetracyclines in animal health, co-resistance to amoxicillin and tetracycline could be an efficient indicator of MDR in isolates. Based on a specific resistance profile and not an arbitrary number of resistances compared with MDR, this potential indicator is also precise, convenient and suitable for routine use.
Copyright © 2019 Bourély, Cazeau, Jarrige, Jouy, Haenni, Lupo, Madec, Leblond and Gay.
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Publication Date: 2019-10-09 PubMed ID: 31649635PubMed Central: PMC6794424DOI: 10.3389/fmicb.2019.02288Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article centers on using the co-resistance of Escherichia coli to common antibiotics Amoxicillin and Tetracycline, as a potential indicator of multi-drug resistance, across various animal species. By studying bacteria samples from French animal health surveillance network between 2012 to 2016, the researchers suggest that this form of resistance shows potential as a reliable, precise, convenient, and routinely usable indicator of multi-drug resistance.

Research Methodology

The following are the key steps employed in the research:

  • The research analyzed the bacterial isolates collected between 2012 and 2016 by the French surveillance network for antimicrobial resistance (RESAPATH).
  • A chief focus of the research was studying the extent of multidrug resistance (MDR) and the co-resistance to Amoxicillin and Tetracycline in these isolates.
  • The samples for the study were taken from seven different animal species, namely cattle, horse, dog, swine, poultry, duck, and turkey.
  • In order to effectively compare and understand the alignment between MDR and co-resistance, the researchers calculated the kappa value, a statistical measure of the degree of agreement between two proportions.

Research Findings

  • A total of 55,904 isolates were analyzed. The extent of multi-drug resistance (MDR) was found to largely vary across different animal species. The MDR proportions ranged from 21.9% in horses to 56% in cattle.
  • Parallelly, the study found the proportions of bacteria demonstrating co-resistance to Amoxicillin and Tetracycline to also exhibit variability across different species with highest in cattle (65%).
  • The co-resistance was seen to be frequently associated with resistance to other antibiotics, regardless of the animal species.
  • Further statistical analysis via kappa value calculation showcased a substantial agreement between MDR and the co-resistance to Amoxicillin and Tetracycline. This occurred across all animal species studied, indicating that the co-resistance could act as a reliable indicator of MDR.

Conclusion

  • The widespread usage of penicillins and tetracyclines in animal health, combined with the observed co-resistance to both drugs, suggests that observing this resistance could be a practical and efficient way to indicate MDR in E.coli isolates.
  • This potential indicator is viewed as being precise, convenient and suitable for routine use, especially given it relies on a specific resistance profile, rather than an arbitrary number of resistances when compared to MDR.

Cite This Article

APA
Bourély C, Cazeau G, Jarrige N, Jouy E, Haenni M, Lupo A, Madec JY, Leblond A, Gay E. (2019). Co-resistance to Amoxicillin and Tetracycline as an Indicator of Multidrug Resistance in Escherichia coli Isolates From Animals. Front Microbiol, 10, 2288. https://doi.org/10.3389/fmicb.2019.02288

Publication

Frontiers in microbiology
ISSN: 1664-302X
NlmUniqueID: 101548977
Country: Switzerland
Language: English
Volume: 10
Pages: 2288
PII: 2288

Researcher Affiliations

Bourély, Clémence
  • École Nationale des Services Vétérinaires, ENSV, VetAgro Sup, Marcy l'Étoile, France.
  • ANSES, Laboratoire de Lyon, Unité Épidémiologie et Appui à la Surveillance, Université de Lyon, Lyon, France.
  • EPIA, UMR 0346, Epidémiologie des Maladies Animales et Zoonotiques, INRA, VetAgro Sup, University of Lyon, Marcy l'Étoile, France.
Cazeau, Géraldine
  • ANSES, Laboratoire de Lyon, Unité Épidémiologie et Appui à la Surveillance, Université de Lyon, Lyon, France.
Jarrige, Nathalie
  • ANSES, Laboratoire de Lyon, Unité Épidémiologie et Appui à la Surveillance, Université de Lyon, Lyon, France.
Jouy, Eric
  • Laboratoire de Ploufragan-Plouzané-Niort, ANSES, Unité Mycoplasmologie Bactériologie Antibiorésistance, Université Bretagne Loire, Technopôle Saint-Brieuc Armor, Ploufragan, France.
Haenni, Marisa
  • ANSES, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactériennes, Université de Lyon, Lyon, France.
Lupo, Agnese
  • ANSES, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactériennes, Université de Lyon, Lyon, France.
Madec, Jean-Yves
  • ANSES, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactériennes, Université de Lyon, Lyon, France.
Leblond, Agnès
  • EPIA, UMR 0346, Epidémiologie des Maladies Animales et Zoonotiques, INRA, VetAgro Sup, University of Lyon, Marcy l'Étoile, France.
Gay, Emilie
  • ANSES, Laboratoire de Lyon, Unité Épidémiologie et Appui à la Surveillance, Université de Lyon, Lyon, France.

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