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Frontiers in veterinary science2020; 7; 631; doi: 10.3389/fvets.2020.00631

Collagen and Eosinophils in Jenny’s Endometrium: Do They Differ With Endometrial Classification?

Abstract: Collagen fibers and inflammatory cells are the basis for jenny endometrium Kenney and Doig's classification developed for the mare. The infiltration of a large number of eosinophils in the jenny endometrium is intriguing. Eosinophil and fibroblast produced IL33, which has been related to fibrosis development and chronicity. This work on the endometrium consisted of (i) quantification of collagen type I (COL1A2), type III (COL3A1), and IL33 transcripts; (ii) histological localization and quantification of COL1 and COL3 proteins; and (iii) eosinophil and neutrophil count and correlation with collagen area and IL33 transcripts. Localization of COL protein in the jenny endometrium was also compared to the mare endometrium. As fibrosis increased, eosinophil and neutrophil count decreased ( < 0.05). A 5-fold increase in IL33 transcripts was noted from categories IIA to III. There was a tendency toward a positive correlation between eosinophil count and IL33 transcripts in category IIA endometrium ( = 0.055). Neither transcripts of COL1A2 nor COL3A1 nor the areas of COL1 or COL3 differed with endometrial categories. Unlike for the mare, and regardless of the jenny endometrium classification, COL3 was always found to different extents in the stratum compactum, while COL1 was mainly present in deep stroma. As fibrosis progressed in the mare, an extensive increase in COL1 fibers was notorious under the surface epithelium. Correlations between neutrophil count and COL1 and COL3 areas were observed in the jenny endometrium, although no correlation was found for eosinophil count. Neutrophil count positive correlation with the COL1 area and negative correlation with the COL3 area in endometria with mild lesions suggest that neutrophils in the jenny endometrium may be involved in fibrogenesis. In addition, when eosinophilia subsides, the endometrium reacts with fibrosis establishment, which could be stimulated by the pro-fibrotic cytokine IL33, whose release might then be ascribed to fibroblasts. Further studies are needed to analyze the effect of the presence of COL3 next to the surface epithelium in the stratum compactum, or around the endometrial glands on jenny's endometrial function and fertility.
Copyright © 2020 Miró, Gutiérrez-Reinoso, Silva, Fernandes, Rebordão, Alexandre-Pires, Catalán and Ferreira-Dias.
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Publication Date: 2020-09-10 PubMed ID: 33134338PubMed Central: PMC7511575DOI: 10.3389/fvets.2020.00631Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the relationship between different types of collagen, inflammatory cells like eosinophils and neutrophils, and interleukin 33 (IL33) in the endometrium of jennies (female donkeys), by comparing it to mares (female horses). The finding suggests that neutrophils may contribute to fibrosis and eosinophils may stimulate the release of IL33, a pro-fibrotic cytokine.

Study objectives and method

  • The overarching goal of this study was to understand better the role of collagen, eosinophils, and IL33 in Jenny endometrium. Jenny endometrium refers to the lining of the uterus in female donkeys, called jennies.
  • The researchers used several methods to achieve this goal. They quantified the presence of collagen type I and type III (known as COL1A2 and COL3A1 respectively) and IL33, a protein associated with immune response and inflammation.
  • They also noted the number of eosinophils and neutrophils, types of white blood cells that are key to the body’s immune response, and looked for any correlations between these cell types and collagen or IL33.

Key findings

  • As fibrotic tissue (scar tissue caused by fibrosis) increased, the study observed a decrease in the count of both eosinophils and neutrophils.
  • Interestingly, a 5-fold increase in the protein IL33 was found between certain categories of fibrosis. There seemed to be a possible correlation between the presence of eosinophils and IL33 in category IIA endometrium.
  • The location of collagen proteins in jenny endometrium differed from mare endometrium (female horse endometrium). While collagen type III was present at different extents in all classifications of jenny endometrium, collagen type I was found mainly in the deeper layers.
  • And although no correlations were found with eosinophil count, correlations were observed between neutrophil count and COL1 and COL3 areas, suggesting that neutrophils could play a role in fibrogenesis (the formation of fibrous tissue).

Takeaway and further studies

  • The findings suggest that as eosinophilia (high eosinophil levels) subsides, the endometrium reacts by developing fibrosis. This action could be stimulated by IL33 and could mean that fibroblasts (a type of cell that makes the extracellular matrix and collagen) are responsible for its release.
  • There are still more studies needed to further understand the effects of collagen type III’s presence near the epithelium surface and the endometrial glands of jenny’s endometrium on fertility and overall endometrial function.

Cite This Article

APA
Miró J, Gutiérrez-Reinoso M, da Silva JA, Fernandes C, Rebordão MR, Alexandre-Pires G, Catalán J, Ferreira-Dias G. (2020). Collagen and Eosinophils in Jenny’s Endometrium: Do They Differ With Endometrial Classification? Front Vet Sci, 7, 631. https://doi.org/10.3389/fvets.2020.00631

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 7
Pages: 631
PII: 631

Researcher Affiliations

Miró, Jordi
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Cerdanyola del Vallès, Spain.
Gutiérrez-Reinoso, Miguel
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Cerdanyola del Vallès, Spain.
da Silva, Joana Aguiar
  • Faculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisbon, Portugal.
Fernandes, Carina
  • Faculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisbon, Portugal.
Rebordão, Maria Rosa
  • Faculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisbon, Portugal.
  • Coimbra College of Agriculture, Polytechnic Institute of Coimbra, Coimbra, Portugal.
Alexandre-Pires, Graça
  • Faculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisbon, Portugal.
Catalán, Jaime
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Cerdanyola del Vallès, Spain.
Ferreira-Dias, Graça
  • Faculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisbon, Portugal.

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This article has been cited 6 times.
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