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Home / Equine Research Bank / Trop Anim Health Prod / Comparative diagnosis of parasitological, serological, and m...
Tropical animal health and production2010; 42(8); 1649-1654; doi: 10.1007/s11250-010-9615-1

Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses.

Abstract: Study on comparative sensitivity of parasitological, serological, and molecular tests on 237 horses originating from two dourine-suspected districts of Arsi-Bale highlands of Ethiopia was conducted to determine the prevalence of the disease and degree of agreement of the diagnostic tests. Accordingly, the prevalence of the disease was found to be 4.6%, 36.7%, and 47.6% by parasitological Woo test, RoTat 1.2 and 18S PCR tests, respectively. The seroprevalence of the disease was 27.6% in CATT/Trypanosoma evansi test. In Ethiopia, it was for the first time that trypanosomes from dourine suspected horses were demonstrated in 4.6% of the animals using Woo test. The findings of the present study disclosed that dourine is highly prevalent and one of the major diseases of horses in the area. There was no statistically significant difference (P>0.05) in prevalence of the disease between districts, sexes, and age groups of the animals. However, there was a statistically significant difference (P<0.05) in the prevalence of the disease between emaciated and animals with good body condition. Assessment of the degree of agreement of the diagnostic tests employed revealed low to fair (k = 0·1 - 0·4) with significantly higher sensitivity by PCR than other tests.
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Publication Date: 2010-06-06 PubMed ID: 20526860DOI: 10.1007/s11250-010-9615-1Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper investigates how effective different kinds of diagnostic tests are at detecting a parasitic disease known as dourine in horses. The study was conducted on 237 horses in Ethiopia and found that the disease is highly prevalent in the region.

Study Design and Methodology

  • The research was conducted on 237 horses from Ethiopia’s Arsi-Bale highlands, which are considered dourine-suspected districts.
  • The diagnostic tools used for comparison included parasitological, serological, and molecular tests. Specifically, these were the Woo test, the RoTat 1.2 and 18S PCR tests, and the CATT/Trypanosoma evansi test.
  • The study aimed to find out the prevalence of dourine in the chosen districts and assess the agreement or consistency among the different diagnostic tests.

Key Findings

  • The prevalence of the disease was different depending on the testing method used; the Woo test identified a prevalence rate of 4.6%, while the RoTat 1.2 and 18S PCR tests identified rates of 36.7% and 47.6%, respectively. The CATT/Trypanosoma evansi test detected a seroprevalence, i.e., the level of the pathogen in the blood, of 27.6%.
  • There were no significant differences in disease prevalence based on district, sex, or age of the animals. However, animals with poor body conditions were significantly more likely to have the disease than those with good body conditions.
  • The study found that horses in the area are at high risk of contracting dourine.

Interpretation of Results and Conclusions

  • This research was the first to demonstrate the presence of trypanosomes, the parasites causing dourine, in dourine-suspecting horses in Ethiopia using the Woo test.
  • The varying prevalence rates suggest that the sensitivity of the diagnostic test used for detection plays a crucial role in assessing disease prevalence. The molecular test, specifically the PCR test, showed significantly higher sensitivity than the other tests.
  • The degree of agreement between the various diagnostic tests was low to fair, further emphasizing the importance of using a range of tests for comprehensive disease detection.

Cite This Article

APA
Gari FR, Ashenafi H, Tola A, Goddeeris BM, Claes F. (2010). Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses. Trop Anim Health Prod, 42(8), 1649-1654. https://doi.org/10.1007/s11250-010-9615-1

Publication

Tropical animal health and production
ISSN: 1573-7438
NlmUniqueID: 1277355
Country: United States
Language: English
Volume: 42
Issue: 8
Pages: 1649-1654

Researcher Affiliations

Gari, Fikru Regassa
  • Faculty of Veterinary Medicine, Addis Ababa University, P. O. Box 34, Debre Zeit, Ethiopia. fikruregassa@yahoo.com
Ashenafi, Hagos
    Tola, Alemu
      Goddeeris, Bruno M
        Claes, Filip

          MeSH Terms

          • Animals
          • Body Constitution
          • DNA Primers / genetics
          • Diagnostic Techniques and Procedures / veterinary
          • Dourine / diagnosis
          • Dourine / epidemiology
          • Ethiopia / epidemiology
          • Horse Diseases / diagnosis
          • Horse Diseases / epidemiology
          • Horses
          • Polymerase Chain Reaction / methods
          • Polymerase Chain Reaction / veterinary
          • Prevalence
          • Sensitivity and Specificity
          • Seroepidemiologic Studies
          • Serologic Tests / veterinary
          • Trypanosoma

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          Citations

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