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Equine veterinary journal2011; 44(1); 33-42; doi: 10.1111/j.2042-3306.2010.00353.x

Comparative study of equine bone marrow and adipose tissue-derived mesenchymal stromal cells.

Abstract: Mesenchymal stromal cells (MSCs) represent an attractive source for regenerative medicine. However, prior to their application, fundamental questions regarding molecular characterisation, growth and differentiation of MSCs must be resolved. Objective: To compare and better understand the behaviour of equine MSCs obtained from bone marrow (BM) and adipose tissue (AT) in culture. Methods: Five horses were included in this study. Proliferation rate was measured using MTT assay and cell viability; apoptosis, necrosis and late apoptosis and necrosis were evaluated by flow cytometry. The mRNA expression levels of 7 surface marker genes were quantified using RT-qPCR and CD90 was also analysed by flow cytometry. Differentiation was evaluated using specific staining, measurement of alkaline phosphatase activity and analysis of the mRNA expression. Results: High interindividual differences were observed in proliferation in both cell types, particularly during the final days. Statistically significant differences in viability and early apoptosis of cultured AT- and BM-MSCs were found. The highest values of early apoptosis were observed during the first days of culture, while the highest percentage of necrosis and late apoptosis and lowest viability was observed in the last days. Surface marker expression pattern observed is in accordance to other studies in horse and other species. Osteogenic differentiation was evident after 7 days, with an increasing of ALP activity and mRNA expression of osteogenic markers. Adipogenic differentiation was achieved in BM-MSCs from 2 donors with one of the 16 media tested. Chondrogenic differentiation was also observed. Conclusions: Proliferation ability is different in AT-MSCs and BM-MSCs. Differences in viability and early apoptosis were observed between both sources and CD34 was only found in AT-MSCs. Differences in their osteogenic and adipogenic potential were detected by staining and quantification of specific tissue markers. Conclusions: To provide data to better understand AT-MSCs and BM-MSCs behaviour in vitro.
© 2011 EVJ Ltd.
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Publication Date: 2011-06-13 PubMed ID: 21668489DOI: 10.1111/j.2042-3306.2010.00353.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on a comparative study between equine bone marrow and adipose tissue-derived mesenchymal stromal cells, aiming to discover potential differences in their proliferation, viability, apoptosis, and differentiation abilities, which could contribute to their utility across various applications in regenerative medicine.

Research Methodology

  • The study involved five horses from which bone marrow (BM) and adipose tissue (AT) were obtained for the isolation and cultivation of mesenchymal stromal cells (MSCs).
  • Various assays and tests were performed to evaluate cell proliferation, viability such as MTT assay and flow cytometry.
  • Cell marker expression was evaluated using RT-qPCR to quantify mRNA expression and flow cytometry to analyze CD90.
  • Cell differentiation abilities were also assessed by measuring alkaline phosphatase activity and analyzing mRNA expression.

Findings of the Study

  • The study revealed notable differences in the proliferation abilities of adipose tissue-derived MSCs (AT-MSCs) and bone marrow-derived MSCs (BM-MSCs).
  • Specific differences were also observed in terms of cell viability and early apoptosis between the two cell types.
  • Early apoptosis was significantly high during the initial days of cell culture, while the latter days observed increased necrosis and late apoptosis, along with decreased cell viability.
  • The patterns of surface marker expression observed in the study aligned with previous research conducted on horses and other species.
  • Furthermore, distinct differences were noticed in osteogenic and adipogenic differentiation potential between AT-MSCs and BM-MSCs.

Conclusion of the Study

  • The research determined that there are observable differences between AT-MSCs and BM-MSCs, especially related to their proliferation capacity, viability, early apoptosis, and tissue differentiation ability.
  • Interestingly, the study found CD34 expression only in the AT-MSCs and not in the BM-MSCs.
  • The results of this study contribute valuable data for future research, aiming to better understand the behavior of these cell types in vitro, which consequently could enhance their potential therapeutic applications in regenerative medicine.

Cite This Article

APA
Ranera B, Ordovás L, Lyahyai J, Bernal ML, Fernandes F, Remacha AR, Romero A, Vázquez FJ, Osta R, Cons C, Varona L, Zaragoza P, Martín-Burriel I, Rodellar C. (2011). Comparative study of equine bone marrow and adipose tissue-derived mesenchymal stromal cells. Equine Vet J, 44(1), 33-42. https://doi.org/10.1111/j.2042-3306.2010.00353.x

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 44
Issue: 1
Pages: 33-42

Researcher Affiliations

Ranera, B
  • Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, Spain.
Ordovás, L
    Lyahyai, J
      Bernal, M L
        Fernandes, F
          Remacha, A R
            Romero, A
              Vázquez, F J
                Osta, R
                  Cons, C
                    Varona, L
                      Zaragoza, P
                        Martín-Burriel, I
                          Rodellar, C

                            MeSH Terms

                            • Adipose Tissue / cytology
                            • Animals
                            • Apoptosis
                            • Bone Marrow Cells / physiology
                            • Cell Differentiation
                            • Cell Proliferation
                            • Cell Survival
                            • Cells, Cultured
                            • Flow Cytometry
                            • Gene Expression Regulation
                            • Horses / physiology
                            • Membrane Proteins / genetics
                            • Membrane Proteins / metabolism
                            • Mesenchymal Stem Cells / physiology
                            • Necrosis
                            • Osteogenesis
                            • RNA, Messenger / genetics
                            • RNA, Messenger / metabolism

                            Citations

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